• Title/Summary/Keyword: expression

Search Result 28,788, Processing Time 0.268 seconds

Different Prognostic Factors Correlate with Bcl-2 Expression among Triple Negative and Non-Triple Negative Breast Cancers

  • El-Mageed, Amal Abd El-Hafez Abd;Shawky Mohamed, Abd El-Aty;Elesawy, Basem Hasan
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.14 no.2
    • /
    • pp.1037-1041
    • /
    • 2013
  • Background: Prognostication of breast cancer using clinico-pathologic variables, although useful, remains imperfect. Recent research has focused on finding new markers of prognosis using gene expression profiling. Panels of proteins assessed by immunohistochemistry might also be useful in this regard. This study focused on Bcl-2 protein expression in triple-negative (TNBC) and non- triple-negative breast cancer (non-TNBC) with correlation to clinico-pathologic variables. Materials and methods: We analyzed Bcl-2 expression in 77 women with primary breast carcinoma divided into two groups; triple-negative and non- triple-negative according to expression of estrogen (ER), progesterone (PR) and human epidermal growth factor receptors (Her2/neu). Bcl-2 expression was assessed in relation to age, histo-pathological subtype, grade, nodal status and tumor size. Results: Bcl-2 was expressed in 74% of triple-negative breast cancers and 70% of non- triple-negative cancers. In TNBC, expression was significantly correlated with invasive ductal subtype, while in non-TNBC it was significantly correlated with age and negative nodal status. In both groups higher Bcl-2 expression associated with favourable prognostic factors in breast cancer, but no significant statistical correlations were found. Conclusions: Frequency of Bcl-2 expression does not differ between TNBC and non-TNBC, but different prognostic factors correlate with Bcl-2 in the two cases.

Ectopic Expression of Caveolin-1 Induces COX-2 Expression in Rabbit Articular Chondrocytes via MAP Kinase Pathway

  • Kim, Song-Ja
    • IMMUNE NETWORK
    • /
    • v.6 no.3
    • /
    • pp.123-127
    • /
    • 2006
  • Background: Caveolin-1 is a principal component of caveolae membranes in vivo. Although expression of caveolae structure and expression of caveolin family, caveolin-1, -2 and -3, was known in chondrocytes, the functional role of caveolae and caveolins in chondrocytes remains unknown. In this study, we investigated the role of caveolin-1 in articular chondrocytes. Methods: Rabbit articular chondrocytes were prepared from cartilage slices of 2-week-old New Zealand white rabbits by enzymatic digestion. Caveolin-1 cDNA was transfected to articular chondrocytes using LipofectaminePLUS. The cyclooxygenase-2 (COX-2) expression levels were determined by immunoblot analysis, immunostaining, immunohistochemistry, and prostaglandin $E_2\;(PGE_2)$ assay was used to measure the COX-2 activity. Results: Ectopic expression of caveolin-1 induced COX-2 expression and activity, as indicated by immunoblot analysis and $PGE_2$ assay. And also, overexpression of caveolin-1 stimulated activation of p38 kinase and ERK-1/-2. Inhibition of p38 kinase and ERK-1/-2 with SB203580 and PD98059, respectively, led to a dose-dependent decrease COX-2 expression and $PGE_2$ production in caveolin-1-transfected cells. Conclusion: Taken together, our data suggest that ectopic expression of caveolin-1 contributes to the expression and activity of COX-2 in articular chondrocytes through MAP kinase pathway.

A Study on the Organic Expression Principles of the 20C. Architecture. (20세기 건축의 유기적 표현원리에 관한 연구)

  • 김주연;우경덕
    • Korean Institute of Interior Design Journal
    • /
    • no.18
    • /
    • pp.51-58
    • /
    • 1999
  • The aim of this study is to discover the organic expression principles of the 20C. Architecture. As a result, first, the organic expression principles was organised in six categories, a. naturalism b. neo-naturalism, c. realism, d. abstractionism, e. regionalism, f. functionalism. Second, among the organic expression principles, the naturalism, the neo-naturalism and the abstractionism were the most dominant organic expression principles of the 20C. Architecture. Third, from 1960, the functionalism have become one of the most dominant organic expression of the 20C. Architecture.

  • PDF

How are Bayesian and Non-Parametric Methods Doing a Great Job in RNA-Seq Differential Expression Analysis? : A Review

  • Oh, Sunghee
    • Communications for Statistical Applications and Methods
    • /
    • v.22 no.2
    • /
    • pp.181-199
    • /
    • 2015
  • In a short history, RNA-seq data have established a revolutionary tool to directly decode various scenarios occurring on whole genome-wide expression profiles in regards with differential expression at gene, transcript, isoform, and exon specific quantification, genetic and genomic mutations, and etc. RNA-seq technique has been rapidly replacing arrays with seq-based platform experimental settings by revealing a couple of advantages such as identification of alternative splicing and allelic specific expression. The remarkable characteristics of high-throughput large-scale expression profile in RNA-seq are lied on expression levels of read counts, structure of correlated samples and genes, larger number of genes compared to sample size, different sampling rates, inevitable systematic RNA-seq biases, and etc. In this study, we will comprehensively review how robust Bayesian and non-parametric methods have a better performance than classical statistical approaches by explicitly incorporating such intrinsic RNA-seq specific features with flexible and more appropriate assumptions and distributions in practice.

Colchicine Inhibits Integrin ${\alpha}_5{\beta}_1$ Gene Expression during PMA induced dDfferentiation of U937 Cells

  • Jang, Won-Hee;Rhee, In-Ja
    • Archives of Pharmacal Research
    • /
    • v.18 no.6
    • /
    • pp.376-380
    • /
    • 1995
  • Monocyte adhesion involves specific cell surface receptors, integrins and results in cell differentiation. We have studied expression and regulation of integrin .${\alpha}_5{\beta}_1$ during differntiation of U937 as in vitro model. To determine expression of integrin ${\alpha}_5{\beta}_1$ during differentiation of U937 as in vitro model. To determine expression of integrin ${\alpha}_5{\beta}_1$ genes by RT-PCR (reverse transcription and polymerase chain reaction) method. We determined expression of integrin ${\alpha}_5{\beta}_1$ genes by RT-PCE (reverse transcription and polymerase chain reaction) method. We found that expression of integrin .alpha.5.betha.1 was greatly increased during PMA-induced differentiation of U937 cells and also found that PMA-induced expression of integrin ${\alpha}_5{\beta}_1$ was inhibited by colchicine, microtubule depoly merizing agent. These results indicate that microtubular integrity is associated with expression of integrin. ${\alpha}_5{\beta}_1$ during PMA-induced differentiation of U937 cells.

  • PDF

A Study on the Introduction of the Regional Tendency into Korean Modern Interior Design (한국현대실내디자인에서 지역성의 구축에 관한 연구)

  • 남경숙
    • Korean Institute of Interior Design Journal
    • /
    • no.27
    • /
    • pp.96-103
    • /
    • 2001
  • The purpose of this thesis is to study on the source, the introductive process and the phenomenon of the regional tendency into Korean modern interior design. To achieve the purpose of this study, the methods and contents are as follows : 1. Explaining the regionalism in the architecture as the source of the regional tendency. 2. Explaining the introductive process of the regional tendency in Korean modern interior design.. For that, 1)Explaining the traditional expression, the leo-corbusian expression and the organic expression with nature in Korean contemporary architecture 2)Explaining the vernacular expression and the abstract expression in Korean modern art 3. Studying the regional tendency in Korean modern interior design For that, 1)Studying the expression of Korean traditionality 2)Studying the expression of the vernacular 3)Studying the expression of the environmental affinity By theme methods and contents, we will study the introduction of the regional tendency into Korean modern interior design.

  • PDF

A Study on the Application Traditional Design Elements for Contemporary Interior Space (현대실내공간에서의 전통의장요소 활용에 관한 연구)

  • Jun, Kyong-Hee;Nam, Kyung-Sook
    • Proceedings of the Korean Institute of Interior Design Conference
    • /
    • /
    • pp.116-121
    • /
    • 2005
  • This research against the method of applying a traditional design elements to modern interior space is classified and analyzed by direct expression method, a transfigure expression method, a metaphor expression method and a symbol expression method. The direct expression method means the modernizing the traditional design elements by expressing traditional material and structural formative style as they are, and the transfigure expression method is the design method of re-organizing the elements by simplifying and transfiguring the traditional design elements. Metaphor expression method includes the method of expressing visually the formative concept which does not known concretely, and the symbol expression method exchanges the rising concepts into concrete elements and expresses by new design.

  • PDF

Enhancing Gene Expression Classification of Support Vector Machines with Generative Adversarial Networks

  • Huynh, Phuoc-Hai;Nguyen, Van Hoa;Do, Thanh-Nghi
    • Journal of information and communication convergence engineering
    • /
    • v.17 no.1
    • /
    • pp.14-20
    • /
    • 2019
  • Currently, microarray gene expression data take advantage of the sufficient classification of cancers, which addresses the problems relating to cancer causes and treatment regimens. However, the sample size of gene expression data is often restricted, because the price of microarray technology on studies in humans is high. We propose enhancing the gene expression classification of support vector machines with generative adversarial networks (GAN-SVMs). A GAN that generates new data from original training datasets was implemented. The GAN was used in conjunction with nonlinear SVMs that efficiently classify gene expression data. Numerical test results on 20 low-sample-size and very high-dimensional microarray gene expression datasets from the Kent Ridge Biomedical and Array Expression repositories indicate that the model is more accurate than state-of-the-art classifying models.

Effects of Ginseng Saponin on the Cytokine Gene Expression in Human Immune System (인삼 사포닌이 인간면역계 사이토카인 유전자의 발현에 미치는 영향)

  • 박종욱;한인숙
    • Journal of Ginseng Research
    • /
    • v.20 no.1
    • /
    • pp.15-22
    • /
    • 1996
  • In order to investigate the Immunomodulatory effects of ginseng, we have studied the effects of ginseng saponin on the proliferation and cytosine gene expression of human pheripheral blood mononuclear cell (PBMC). In the PBMC proliferation assay, total saponin exhibited proliferation inhibition on the PBMC or phytohemagglutinin(PHA)-stimulated PBMC in a dose-dependent fashion. Immunomodulatory effects of ginseng were further investigated using the cytokine gene expression as the indicators. In the reverse transcription-polymerase chain reaction (RT-PCR) test, interleukin (IL)-1, IL-2, IL-3, IL-4, IL-6, IL-13, granulocyte macrophage-colony stimulating factor, tumor necrosis factor (TNF), migration inhibitory factor and transforming growth factor genes were expressed in the PHA-stimulated PBMC 48 hrs after cell culture. Among expressed cytokines, total saponin could increase the expression of IL-1 and TNF of PBMC without stimulation of PHA. All of ginsenosides, $Rb_1$, $Rb_2$, $Rg_1$, Rc, Re, incresed TNF gene expression. Especially, Rb2 (20 g/ml) showed most prominent effect on TNF gene expression and it also slightly increased IL-1 gene expression of PBMC.

  • PDF