• Title/Summary/Keyword: expression

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A Study the Elements of Technological Specific Expression in Research Institute Architecture -Focused on the Contemporary Research Institutes- (연구소 건축 계획의 테크놀로지 특성 표현 요소에 관한 연구 - 현대 연구소 건축을 중심으로 -)

  • Kim Hwan-Sik;Lee Jeong-Soo;Song Yong-Ho
    • Korean Institute of Interior Design Journal
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    • v.14 no.6
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    • pp.168-176
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    • 2005
  • This study is a understanding on technological specific expression to reflect of a research institute in modern architecture. For this study, it separately reduce space program, plan program, design elements of elevation, structural system, mechanical system, building material program to its expressional elements of research institute. Also, it's arranged about a specificity that is expressed for technology each plan elements of a research institute. As a result, elements what is expressive of technology on research institute are influenced an aesthetic expression, expression of an arrangement system, structural expression, expression of architectural organization, and it influences more getting feels technological specificity expressed an aesthetic, structural system, architectural composition, mechanical distribution system in order of their magnitude. This is offered suggestion what must be an achievable complex both a simply technological expression and aesthetic expression.

Intragenic Control of Expression of a Rice MADS Box Gene OsMADS1

  • Jeon, Jong-Seong;Lee, Sichul;An, Gynheung
    • Molecules and Cells
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    • v.26 no.5
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    • pp.474-480
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    • 2008
  • OsMADS1 is a rice MADS box gene necessary for floral development. To identify the key cis-regulatory regions for its expression, we utilized transgenic rice plants expressing GUS fusion constructs. Histochemical analysis revealed that the 5.7-kb OsMADS1 intragenic sequences, encompassing exon 1, intron 1, and a part of exon 2, together with the 1.9-kb 5' upstream promoter region, are required for the GUS expression pattern that coincides with flower-preferential expression of OsMADS1. In contrast, the 5' upstream promoter sequence lacking this intragenic region caused ectopic expression of the reporter gene in both vegetative and reproductive tissues. Notably, incorporation of the intragenic region into the CaMV35S promoter directed the GUS expression pattern similar to that of the endogenous spatial expression of OsMADS1 in flowers. In addition, our transient gene expression assay revealed that the large first intron following the CaMV35S minimal promoter enhances flower-preferential expression of GUS. These results suggest that the OsMADS1 intragenic sequence, largely intron 1, contains a key regulatory region(s) essential for expression.

A Study on Expression Method and Characteristic of Transparency of the Surface in Current Commercial Space (현대 상업건축공간에 있어서 표피의 투명성 표현방법과 이미지 특성에 관한 연구)

  • Park Chan-Il;Cho Mi-Na
    • Korean Institute of Interior Design Journal
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    • v.14 no.6
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    • pp.66-74
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    • 2005
  • The purpose of this study analyzes an expression method and a characteristic of transparency of a surface in commercial space, and it is to show an index of a surface design. We did image evaluation by SD method for the commercial space which directed transparency to a surface as the method. We used a factor analysis and cluster analysis to get the image characteristic and type of the expression methods. As a result, we got six following expression methods and design indexes. (1) Transparency expression of a surface by a multiple layer. (2) Transparency expression of a surface by unification with a structure. (3) Transparency expression of a surface by transparency of materials. (4) Transparency expression of a surface by a combination of various materials. (5) Transparency expression of a surface by unification of the image media. (6) Transparency expression of a surface by transparency transformation of materials. We think that it will be able to make use of these results as a design index for the surface design of a commercial space in the future.

Effect of Transcription Terminators on Expression of Human Lipocortin-1 in Recombinant Saccharomyces cerevisiae

  • Chung, Bong-Hyun;Kim, Byung-Moon;Nam, Soo-Wan;Park, Young-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.237-244
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    • 1994
  • The vector systems for the expression and secretion of human lipocortin-l (LC1) from Saccharomyces cerevisiae were constructed with GAL10 promoter and the prepro leader sequence of mating factor-$\alpha$1. They were further constructed to contain three different transcription terminators; GAL7 terminator, LCl terminator and a fused form of these two terminators. The expression and secretion levels of LCl were compared to investigate the effect of transcription terminators on the LCl gene expression. For the expression cassettes employing the GAL7 terminator or the terminator of fused form, the expression levels of LCl were measured by scanning the immunoreactive LCl protein bands, and were found to be 0.27 g/l and 0.32 g/l, respectively. The highest expression level of 0.54 g/l was obtained with the expression vector containing the LCl transcription terminator. In all expression cassettes, the majority of LCl proteins expressed were retained intracellularly, indicating a low secretion efficiency of about 5%. The high expression level of LCl was explained by the great content and stability of LCl mRNA transcribed from the LCl terminator-employing vector. The results of this study demonstrate that the LCl transcription terminator functions for the expression of LCl in S. cerevisiae better than the GAL7 terminator.

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Considerations for BIBFRAME Acceptance of Expression and Representative Expression Attributes in LRM (BIBFRAME에서 LRM 표현형 및 대표표현형 속성 적용시 고려사항)

  • Lee, Mihwa
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.30 no.2
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    • pp.33-50
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    • 2019
  • Cataloging principles, cataloging rules, and encoding formats should considered LRM acceptance because LRM replaced FRBR as the conceptual model. This study identifies considerations for BIBFRAME acceptance of expression and representative expression attributes in LRM by using literature reviews and expert interviews. Primarily, work in BIBFRAME without expression as entity could map to work and expression of LRM and sustain expression by linking 2 works (work and expression). Second, BIBFRAME must consider association between representative expression attributes and specific expressions whose values can be transferred to the representative expression attributes. Third, representative expression attributes are different according to work types in LRM, and language, media, intended audience, and scale, that can be used as representative expression attributes in BIBFRAME, should be changed in class. Fourth, relation properties should be articulated for expanding networks between expressions originated from work in BIBFRAME. This study analyzes LRM and BIBFRAME by focusing on expression entity and representative expression attributes. More LRM study is needed on cataloging principles and cataloging rules.

Development of an RNA Expression Platform Controlled by Viral Internal Ribosome Entry Sites

  • Ko, Hae Li;Park, Hyo-Jung;Kim, Jihye;Kim, Ha;Youn, Hyewon;Nam, Jae-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.29 no.1
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    • pp.127-140
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    • 2019
  • Since 1990, many nucleic acid expression platforms consisting of DNA or RNA have been developed. However, although RNA expression platforms have been relatively neglected, several such platforms capped at the 5' end of RNA by an anti-reverse cap analog have now been developed. At the same time, the capping reaction is a bottleneck in the production of such platforms, with high cost and low efficiency. Here, we investigated several viral and eukaryotic internal ribosome entry sites (IRESs) to develop an optimal RNA expression platform, because IRES-dependent translation does not require a capping step. RNA expression platforms constructed with IRESs from the 5' untranslated regions of the encephalomyocarditis virus (EMCV) and the intergenic region of the cricket paralysis virus (CrPV) showed sufficient expression efficiency compared with cap-dependent RNA expression platforms. However, eukaryotic IRESs exhibited a lower viral IRES expression efficiency. Interestingly, the addition of a poly(A) sequence to the 5' end of the coxsackievirus B3 (CVB3) IRES (pMA-CVB3) increased the expression level compared with the CVB3 IRES without poly(A) (pCVB3). Therefore, we developed two multiexpression platforms (termed pMA-CVB3-EMCV and pCrPV-EMCV) by combining the IRESs of CVB3, CrPV, and EMCV in a single-RNA backbone. The pMA-CVB3-EMCV-derived RNA platform showed the highest expression level. Moreover, it clearly exhibited expression in mouse muscles in vivo. These RNA expression platforms prepared using viral IRESs will be useful in developing potential RNA-based prophylactic or therapeutic vaccines, because they have better expression efficiency and do not need a capping step.

Correlation between Expression Level of Gene and Codon Usage

  • Hwang, Da-Jung;Han, Joon-Hee;Raghava, G P S
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • pp.138-149
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    • 2004
  • In this study, we analyzed the gene expression data of Saccharomyces cerevisiae obtained from Holstege et al. 1998 to understand the relationship between expression level and nucleotide sequence of a gene. First, the correlation between gene expression and percent composition of each type of nucleotide was computed. It was observed that nucleotide 'G' and 'C' show positive correlation (r ${\geq}$ 0.15), 'A' shows negative correlation (r ${\approx}$ -0.21) and 'T' shows no correlation (r ${\approx}$ 0.00) with gene expression. It was also found that 'G+C' rich genes express more in comparison to 'A+T' rich genes. We observed the inverse correlation between composition of a nucleotide at genome level and level of gene expression. Then we computed the correlation between dinucleotides (e.g. AA, AT, GC) composition and gene expression and observed a wide variation in correlation (from r = -0.45 for AT to r = 0.35 for GT). The dinucleotides which contain 'T' have wide range of correlation with gene expression. For example, GT and CT have high positive correlation and AT have high negative correlation. We also computed the correlation between trinucleotides (or codon) composition and gene expression and again observed wide range of correlation (from r = -0.45 for ATA r = 0.45 for GGT). However, the major codons of a large number of amino acids show positive correlation with expression level, but there are a few amino acids whose major codons show negative correlation with expression level. These observations clearly indic ate the relationship between nucleotides composition and expression level. We also demonstrate that codon composition can be used to predict the expression of gene in a given condition. Software has been developed for calculating correlation between expression of gene and codon usage.

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Homeobox Gene (OSH1) Expression in Embryonic Mutants of Rice (Oryza sativa L.)

  • Hong, Soon-Kwan;Lee, Sang-Lyung;Shin, Young-Boum;Yoon, Kyung-Min;Kim, Nam-Soo
    • Animal cells and systems
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    • v.2 no.1
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    • pp.81-86
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    • 1998
  • Recent identification and characterization of plant homeobox genes suggest that they play important roles in morphogenetic events. OSH1, one of the rice homeobox genes, is thought to be related to organ development since the changes of OSH1 gene expression cause morphological abnormalities of leaves by the ectopic expression and is expressed during early embryogenesis. In this experiment, the expression pattern of OSH1 was analyzedinmutants by in situ hybridization, and OSH1's potential as a molecular marker was explored. Region-specific expression of OSH1 during early embryogenesis shows that OSH1 could be used as a molecular marker for characterizing embryo mutants. Although several organless and shootless mutants showed normal expression of OSM1, some mutants exhibited abnormal expression patterns. In a minute organless cle1-1 embryo whose epidermis resembled morphologically the epithelium of scutellum, OSH1 expression was limited to a small basal region. This expression pattern suggests the gross deletion of the basal part. In a radicleless mutant, odm115, OSH1 expression was detected in a basal region instead of subcentral region of the ventral side. Together with other characteristics (short embryo and normal adventitious roots), odm115 was estimated to be derived from the deletion of basal region. Among five shootless mutants, three showed normal expression of OSH1. In the shl2 embryo, no expression of OSH1 was observed. In the shl1 embryo, however, OSH1 expression was extended to a dorsal side, indicating that SHL2 might be related to dorsoventral patterning. The above results of in situ hybrydization clearly indicate that OSH1 can be utilized as a marker for characterizing gene functions of embryo mutants.

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Endo-sulfatase Sulf-1 Protein Expression is Down-regulated in Gastric Cancer

  • Gopal, Gopisetty;Shirley, Sundersingh;Raja, Uthandaraman Mahalinga;Rajkumar, Thangarajan
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.2
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    • pp.641-646
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    • 2012
  • In our recent report on gene expression in gastric cancer we identified the endo-sulfatase Sulf-1 gene to be up-regulated in gastric tumors relative to apparently normal (AN), and paired normal (PN) gastric tissue samples. In the present report we investigate the protein expression levels of Sulf-1 gene in gastric tumors, AN and PN samples using tissue microarray (TMA) and immunohistochemistry. Expression data was collected from two sets of TMA's containing replicate sections of tissue samples. Scoring data from TMA set-1 revealed a significant difference in Sulf-1 immunoreactivity between tumors and "normals" (PN and AN) (p-value = 0.001928). Also, Sulf-1 expression in tumors was also significantly different from either PN (p-value = 0.019) or AN (p-value = 0.006) samples. Similar results were obtained from analysis of scoring data from the second set of arrays. Comparison of mRNA expression and protein expression in gastric tumor tissues revealed that in 6/20 (30%) tumor samples showed up-regulated protein expression concordant with over-expression of mRNA. However, a discord with mRNA being over-expressed relative to down regulated protein expression was observed in majority 14/20 (70%) of tumor samples. Our study indicates down regulation of Sulf-1 protein expression in gastric tumors relative to PN and AN samples which is discordant with mRNA over-expression seen in tumors.