• Title, Summary, Keyword: evodiamine

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Inhibition of Monoamine Oxidase by Evodiamine (Evodiamine의 Monoamine Oxidase 활성 저해작용)

  • Lee, Sang-Seon;Hwang, Bang-Yeon;Ro, Jai-Seup;Lee, Myung-Koo
    • Korean Journal of Pharmacognosy
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    • v.37 no.4
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    • pp.320-323
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    • 2006
  • The effects of evodiamine on monoamine oxidase (MAO) activity were investigated. MAO was purified from mouse brain and the $K_m\;and\;V_{max}$ values of MAO were $78.5{\pm}5.28{\mu}M$ and $0.68{\pm}0.07$ nmol/min/mg protein, respectively (n=4). Evodiamine at $30-120{\mu}M$ showed an inhibitory effect on MAO activity using a substrate kynuramine with an $IC_{50}$ value of $104.2{\mu}M$ (n=4). Evodiamine also exhibited a non-competitive inhibition on MAO. The $K_i$ value for evodiamine was $72.5{\pm}10.8{\mu}M$ (n=4). These results suggest that evodiamine partially contributes to the regulation of monoamine content.

Evodiamine Reduces Caffeine-Induced Sleep Disturbances and Excitation in Mice

  • Ko, Yong-Hyun;Shim, Kyu-Yeon;Lee, Seok-Yong;Jang, Choon-Gon
    • Biomolecules & Therapeutics
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    • v.26 no.5
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    • pp.432-438
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    • 2018
  • Worldwide, caffeine is among the most commonly used stimulatory substances. Unfortunately, significant caffeine consumption is associated with several adverse effects, ranging from sleep disturbances (including insomnia) to cardiovascular problems. This study investigates whether treatment with the Evodia rutaecarpa aqueous extract (ERAE) from berries and its major molecular component, evodiamine, can reduce the adverse caffeine-induced sleep-related and excitation effects. We combined measurements from the pentobarbital-induced sleep test, the open field test, and the locomotor activity test in mice that had been dosed with caffeine. We found that ERAE and evodiamine administration reduced the degree of caffeine-induced sleep disruption during the sleep test. Additionally, we found that evodiamine significantly inhibits caffeine-induced excitation during the open field test, as well as decreasing hyperlocomotion in the locomotor activity test. Additional in vitro experiments showed that caffeine administration decreased the expression of ${\gamma}$-aminobutyric acid $(GABA)_A$ receptor subunits in the mouse hypothalamus. However, evodiamine treatment significantly reversed this expression reduction. Taken together, our results demonstrate that ERAE and its major compound, evodiamine, provide an excellent candidate for the treatment or prevention of caffeine-induced sleep disturbances and excitatory states, and that the mechanism of these beneficial effects acts, at least in part, through the $GABA_A$-ergic system.

Isolation and Quantitative Analysis of Evodiamine from Evodiae Fructus (시판 오수유로부터 evodiamine의 분리 및 함량분석)

  • Hwang, Suk-Yeon;Hwang, Bang-Yeon;Ju, Hei-Kyoung;Park, Jeong-Hill;Son, Kun-Ho;Lee, Seung-Ho;Chang, Seung-Yeup;Kang, Shin-Jung;Ro, Jai-Seup;Lee, Kyong-Soon
    • Korean Journal of Pharmacognosy
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    • v.32 no.2
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    • pp.98-102
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    • 2001
  • Evodiae Fructus has been used in traditional medicine for the treatment of gastro- intestinal disorders, headache and migraine, and as a cardiotonic and analgesic. For the quality control of this crude drug, evodiamine was isolated from the methylene chloride extract of Evodia officinalis (Rutaceae) and identified by the spectroscopic evidences. A quantitative analysis of evodiamine using HPLC method exhibited that the average contents of evodiamine were $0.84{\pm}0.60%$ in 53 samples collected throughout the various regions of Korea.

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Anti-Inflammatory Principles from the Fruits of Evodia rutaecarpa and Their Cellular Action Mechanisms

  • Choi Yong-Hwan;Shin Eun-Myoung;Kim Yeong-Shik;Cai Xing-Fu;Lee Jung-Joon;Kim Hyun-Pyo
    • Archives of Pharmacal Research
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    • v.29 no.4
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    • pp.293-297
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    • 2006
  • The fruits of Evodia rutaecarpa Benth (Rutaceae) has long been used for inflammatory disorders and some anti-inflammatory actions of its constituents such as dehydroevodiamine, evodiamine and rutaecarpine were previously reported. Since the pharmacological data is not sufficient to clearly establish the scientific rationale of anti-inflammatory medicinal use of this plant material and the search for its active principles is limited so far, three major constituents (evodiamine, rutaecarpine, goshuyuamide II) were evaluated for their anti-inflammatory cellular action mechanisms in the present study. From the results, evodiamine and rutaecarpine were found to strongly inhibit prostaglandin $E_2$ synthesis from lipopolysaccharide-treated RAW 264.7 cells at $1-10{\mu}M$. Evodiamine inhibited cyclooxygenase-2 induction and NF-kB activation, while rutaecarpine did not. On the other hand, goshuyuamide II inhibited 5-lipoxygenase from RBL-1 cells $(IC_{50}=6.6{\mu}M)$, resulting in the reduced synthesis of leukotrienes. However, these three compounds were not inhibitory against inducible nitric oxide synthase-mediated nitric oxide production from RAW cells up to $50{\mu}M$. These pharmacological properties may provide the additional scientific rationale for anti-inflammatory use of the fruits of E. rutaecarpa.

Effect of Methanol Extract Concentration on the Anti-oxidative Activity and Toxicity of Evodiae Fructus to AGS Cells (오수유의 메탄올 추출 농도에 따른 항산화와 AGS세포에 대한 독성 효과)

  • Yang, Ji Yeong;Byeon, Hwiyong;Kim, Jin Woo;Kim, Sa Hyun;Lee, Pyeongjae
    • Journal of the Korean Society of Food Culture
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    • v.35 no.4
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    • pp.400-405
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    • 2020
  • Evodiae Fructus is the dried unripe fruit of Evodia rutaecarpa, and has traditionally been used for treating stomachache and diarrhea. Evodiamine and rutaecarpine, the major biologically active compounds of Evodiae Fructus, are reported to have anti-oxidative and anti-inflammatory effects, as well as inhibit proliferation and metastasis of various cancer cells. The current study investigates the anti-oxidative and anti-cancer effects of the Evodiae Fructus extract, considering varying concentrations of methanol extraction (40, 80, and 95%). High contents of total phenolic compounds were determined in the order of extracts 80, 95, and 40%. Evaluating contents of the 95, 80, and 40% extracts revealed 36.77, 7.29, and 1.86 ㎍/mg evodiamine, respectively, and 53.02, 17.16, and 3.79 ㎍/mg rutaecarpine, respectively, with the highest content of both compounds obtained in the 95% extract. DPPH radical scavenging activity was observed to be inversely proportional to the contents of total phenolic compounds, with decreasing SC50 values obtained in the order 80, 95, and 40% extract. The 95 and 80% extracts exerted toxicity to AGS gastric cancer cells, but the 40% extract was non-toxic. Evodiamine is a known anti-cancer agent, and could be responsible for the observed toxicity. Cleavage of PARP, and Caspase-3, -7, -8 and -9 was observed in the 95% extract-treated AGS cells, indicating that cell toxicity exerted by the 95% extract could be attributed to apoptosis.

Isolation of Modulators for Multidrug Resistance from the Fruits of Evodia officinalis (약용식물 오수유로부터 다제약제 내성 조절물질의 분리)

  • Lee, Sung-Woo;Hwang, Bang-Yeon;Kim, Se-Eun;Kim, Hwan-Mook;Kim, Young-Ho;Lee, Kyong-Soon;Lee, Jung-Joon;Ro, Jai-Seup
    • Korean Journal of Pharmacognosy
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    • v.26 no.4
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    • pp.344-348
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    • 1995
  • Preliminary screening test of modulators for multidrug resistance with 400 medicinal plants was carried out by using human multidrug resistance cell line, KB-V1. Among active medicinal plants, the unripe fruits of Evodia officinalis showed a potent modulating activity of MDR. From MeOH extract of this plant, we isolated two indole alkaloids, rutaecarpine (1) and evodiamine (2), by repeated silicagel column chromatography. Rutaecarpine increased the cytotoxicities of vinblastine and taxol against multidrug resistance cells, but evodiamine showed no modulating activity in spite of its potent cytotoxic activities.

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Cyclooxygenase-2 Inhibitor from Evodia rutaecarpa

  • Kang, Sam-Sik;Kim, Ju-Sun;Son, Kun-Ho;Kim, Hyun-Pyo;Chang, Hyeun-Wook
    • Natural Product Sciences
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    • v.5 no.2
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    • pp.65-69
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    • 1999
  • By bioassay guided fractionation followed by chromatographic separation of the MeOH extract from the fruit of Evodia rutaecarpa (Juss.) Benth. (Rutaceae), a new cyclooxygenase-2 inhibitor was isolated and identified as an alkaloid, rutaecarpine. Other alkaloids such as evodiamine and dehydroevodiamine together with limonoids were also isolated and characterized.

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Quantification of the 25 Components in Onkyung-Tang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (UPLC-ESI-MS/MS를 이용한 온경탕 중 25종 성분의 함량분석)

  • Seo, Chang-Seob;Shin, Hyeun-Kyoo
    • Korean Journal of Pharmacognosy
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    • v.47 no.1
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    • pp.92-101
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    • 2016
  • In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was established for simultaneous determination of the 25 marker components, including chlorogenic acid, gallic acid, oxypaeoniflorin, homogentisic acid, methyl gallate, caffeic acid, 3,4-dihydroxybenzaldehyde, paeoniflorin, albiflorin, liquiritin, nodakenin, ferulic acid, ginsenoside Rg1, liquiritigenin, coumarin, cinnamic acid, benzoylpaeoniflorin, ginsenoside Rb1, cinnamaldehyde, paeonol, glycyrrhizin, 6-gingerol, evodiamine, rutecarpine, and spicatoside A in traditional Korean formula, Onkyung-tang. All analytes were separated on a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) at $45^{\circ}C$ using a mobile phase of 0.1% (v/v) formic acid in water and acetonitrile with gradient elution. The MS analysis was carried out using a Waters ACQUITY TQD LC-MS/MS coupled with an electrospray ionization (ESI) source in the positive and negative modes. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}L$, respectively. The correlation coefficient of all analytes in the test ranges was greater than 0.98. The limits of detection and quantification values of the 25 marker compounds were in the ranges 0.03-19.43 and 0.09-58.29 ng/mL, respectively. As a result, methyl gallate, 3,4-dihydroxybenzaldehyde, evodiamine, and rutecarpine were not detected in this sample and the concentrations of the 21 compounds except for the above 4 compounds were $33.09-3,496.32{\mu}g/g$ in Onkyung-tang decoction. Among these compounds, paeonol was detected at the highest amount as a $3,496.32{\mu}g/g$.

Isolation and Determination of Alkaloids in Evodiae Fructus by forming Complex Compound (착 화합물 생성에 의한 오수유 중 알칼로이드의 분리와 정량)

  • Kim, Sung-Eun;Kim, Dae-Keun;Shin, Tae-Yong;Lim, Jong-Pil;Eom, Dong-Ok
    • Korean Journal of Pharmacognosy
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    • v.34 no.3
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    • pp.206-209
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    • 2003
  • The fruit of Evodia officinalis (Rutaceae) is known for containing a number of indoloquinazoline and quinoline type alkaloids. Evodiamine, evocarpine and rutaecarpine are the major constituents of Evodiae Fructus. These alkaloids were isolated and determined by forming complex compounds from Evodiea Fructus. For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate [II] complex ion. The absorbance of alkaloidal complex in 1.2-dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Evodia Fruit was linear over the concentration range of $1.0{\sim}6.0\;mg/ml$. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Evodiae Fructus.

Studies on the Processing of Herbal Medicines (II) -HPLC Analysis of Standard Compounds of Unprocessed and Processed Herbal Medicines- (한약재 수치에 관한 연구(II) -오수유,황기의 수치전.후 지표물질의 함량분석-)

  • Kim, Hyeun-Jeong;Ma, Jin-Yeul;Kim, Jong-Moon;Kim, Jin-Sook
    • Korean Journal of Pharmacognosy
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    • v.33 no.4
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    • pp.305-307
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    • 2002
  • Evodiae Fructus and Astragali Radix were processed according to Chinese pharmacopoeia and traditional literatures. The content of formononetin in processed Astragali Radix was significantly decreased (p<0.05) than that of unprocessed one.