• Title, Summary, Keyword: ethyl acetate fraction

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Anti-oxidative Effects of Ethyl acetate Fraction of Saururus chinensis in Caenorhabditis elegans (삼백초 Ethyl acetate 분획물의 예쁜꼬마선충 내의 항산화 효과)

  • Lee, Eun Byeol;Kim, Jun Hyeong;Lee, Jae-Gwang;Han, Sooncheon;Park, Hyun Mee;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.48 no.1
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    • pp.63-69
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    • 2017
  • Saururus chinensis (Lour.) Baill. (Saururaceae) has been used as a traditional medicine for the treatment of dysuria, leukorrhea, eczema, jaundice and ascites in Korea, China and Japan. Ethanol extract of S. chinensis was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities of ethyl acetate fraction, we checked the activities of superoxide dismutase (SOD) and catalase, and intracellular ROS level and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of ethyl acetate fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, ethyl acetate fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, ethyl acetate fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

Effect of Antioxidation and Inhibition of Melanogenesis from Ligularia stenocephala extract (곤달비추출물의 항산화와 멜라닌 생성 저해 효과)

  • Roh, Eon-Joo;Kim, Yoon-Shin;Kim, Beung-Gwan
    • Journal of the Korean Applied Science and Technology
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    • v.26 no.1
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    • pp.87-92
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    • 2009
  • In this study, we evaluated anti-oxidation and whitening effects of Ligularia stenocephala extract for use as the cosmeceuticals. L. stenocephala was extracted by three different solvents which was n-Hexane, ethyl acetate, $H_{2}O$. The free radical (1,l-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of extract of L. stenocephala was in the order: ethyl acetate fraction of leaf ($IC_{50}$ value of 10.512ug/mL) > ethyl acetate fraction of stem ($IC_{50}$ value of 31.877ug/mL) > $H_{2}O$ fraction of leaf ($IC_{50}$ value of 129.194ug/mL). Reactive oxygen species (ROS) scavenging activity of extract of L. stenocephala was in the order: ethyl acetate fraction of leaf ($IC_{50}$ value of 0.230mg/mL) > ethyl acetate fraction of stem ($IC_{50}$ value of 0.528mg/mL) > $H_{2}O$ fraction of leaf ($IC_{50}$ value of 0.799mg/mL). Tyrosinase inhibition activity of L. stenocephala extracts was reduced 29.477% on ethyl acetate fraction of leaf, 13.583% on ethyl acetate fraction of stems. Therefore, L. stenocephala extracts may be useful as a new antioxidant and whitening agent to inhibit melanogenesis.

Anti-diabetic and Anti-oxidative Effects of Opuntia humifusa Cladodes (천년초 선인장 줄기의 항당뇨 및 항산화 효과)

  • Lee, Ji-Na;Kim, Hyeong-Eun;Kim, Yong-Suk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.5
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    • pp.661-667
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    • 2014
  • To investigate the anti-diabetic and antioxidant effects of Opuntia humifusa cladodes, O. humifusa cladodes powder was extracted with 75% ethanol and fractionated with various solvents. Among the extracts fractionated with various solvents, ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibitory activities of O. humifusa cladodes were highest ($89.94{\pm}1.15%$ and $29.01{\pm}3.03%$, respectively) in the ethyl acetate fraction. Further, total phenolic and flavonoid contents of the ethyl acetate fraction were the highest ($196.02{\pm}5.26$ and $114.00{\pm}10.03{\mu}g/mg$, respectively). DPPH and ABTS radical scavenging activities increased according to the concentration of O. humifusa cladodes extract, and those of the ethyl acetate fraction were the highest. Ferric reducing antioxidant powers of chloroform and the ethyl acetate fraction were higher than those of other fractions. Overall, the ethyl acetate fraction of O. humifusa cladodes showed the highest anti-diabetic and antioxidant effects. Results indicate that O. humifusa cladodes powder has potential as a useful ingredient with anti-diabetic and antioxidant effects.

The Effects of Fractions of Houttuynia cordata THUNB on the Accumulation of Cadmium and Induction of Metallothionein in Rats(IV) (어성초 분획이 흰쥐 장기내 카드뮴 축적과 Metallothionein 생성에 미치는 영향(VI))

  • 이기남;정재열;송용선;이정호;유일수;김신기;백승화
    • The Journal of Korean Medicine
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    • v.22 no.1
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    • pp.22-32
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    • 2001
  • This study was conducted to investigate the antitoxic effects of Houttuynia cordata THUNB with chloroform and ethyl acetate fractions. The results were as follows: 1. Detoxication effects of chloroform and ethyl acetate fractions of Houttuynia cordata THUNB were increased in proportion to the dosages. Detoxication effects of ethyl acetate fraction of Houttuynia cordata THUNB were higher than chloroform fraction of Houttuynia cordata THUNB's results and detoxication effects in the kidneys were higher than results for the liver. 2. Metallothionein concentrations in the liver were higher than concentrations in the kidneys, and ethyl acetate fraction of Houttuynia cordata THUNB was higher than chloroform fraction of Houttuynia cordata THUNB in induction of metallothionein. 3. After the administration of chloroform and ethyl acetate fractions of Houttuynia cordata THUNB, body weights increased in proportion to chloroform and ethyl acetate fraction s dosage of Houttuynia cordata THUNB but changes of body weight dminished after 3 weeks. From the above results, this study suggests that chloroform and ethyl acetate fraction of Houttuynia cordata THUNB increased metallothionein induction to cadmium intoxication in rat s kidney and liver and decreased the toxicity of cadmium in rats.

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Antioxidant Activity of Ethyl acetate Fraction of Mallotus japonicus Twigs in Caenorhabditis elegans (예덕나무 가지의 Ethyl Acetate 분획물의 예쁜 꼬마선충 내의 항산화 효과)

  • Kim, Jun Hyeong;Choi, Hye young;Jung, Jung Eun;Kang, Yeon Ju;Kim, Sun Ju;Kwon, Kang Mu;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.51 no.2
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    • pp.115-121
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    • 2020
  • Caenorhabditis elegans model system was used to investigate the antioxidant activity of methanol extract of Mallotus japonicus twigs. The ethyl acetate soluble fraction of the M. japonicus methanol extract showed the best DPPH radical scavenging activity. The ethyl acetate fraction was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species (ROS) level. In addition, to confirm that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction, SOD-3 expression was measured using a transgenic strain. As a result, the ethyl acetate fraction increased SOD and catalase activity, and decreased ROS accumulation in a dose-dependent manner. In addition, the ethyl acetate fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.

Nitric Oxide Production and Elastase Inhibitory Activities of Extract and Its Fraction from Phellodendri Cortex (황백추출물 및 분획물의 Nitric Oxide 생성 억제 효과 및 Elastase 억제 효과)

  • Um, Ji Na;Min, Jin Woo;Joo, Kwang Sik;Kang, Hee Cheol
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.1
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    • pp.22-28
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    • 2017
  • Background: To date, the anti-wrinkle efficacy of phellodendri cortex has not been defined. In this study, we investigated the nitric oxide (NO) production and elastase inhibitory activities of 80% methanol extract of Phellodendri cortex and its ethyl acetate fraction. Methods and Results: We prepared 80% methanol extract, and its fractions from phellodendri cortex. The treatment of RAW 264.7 cell with $25{\mu}g/m{\ell}$ 80% methanol extract and ethyl acetate fraction resulted in no toxicity. We conducted assays of nitric oxide (NO) production and elastase inhibition. In the NO production assay, the ethyl acetate fraction showed an inhibitory effect approximately 17 times stronger than the 80% methanol extract. In elastase inhibitory assay, the ethyl acetate fraction also showed a stronger effect than the 80% methanol extract. In order to standardize the extract and fraction, we used TLC to separate the extract and observed the plate under UV light. We confirmed that the known pharmacological ingredients berberine, and palmatine in the 80% methanol extract and the ethyl acetate fraction. Conclusions: These results indicated that phellodendri cortex extract and its ethyl acetate fraction produced strong inhibitory effect on elastase and NO production.

Antimicrobial Activity of Extracts and Fractions of Green Tea Used for Coarse Tea (엽차용 녹차 추출물 및 분획물의 항균효과)

  • Chung, Sook-Hyun;Yoon, Kyo-Hie
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.11
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    • pp.1382-1388
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    • 2008
  • Antimicrobial activities of green tea extracts used for coarse tea were investigated by disc diffusion method using eight different bacteria. Among the green tea extracts, the 70% ethanol extract demonstrated the strongest antimicrobial activities against Vibrio parahemolyticus (V. parahemolyticus) and Staphylococcus aureus (S. aureus) and thus was further fractionated. Among these fractions, the ethyl acetate fraction showed the strongest antimicrobial activities against V. parahemolyticus, S. aureus, Bacillus subtilis (B. subtilis), and Streptococcus mutans (S. mutans). These activities exceeded that of all extracts and fractions tested in this study. Interestingly, although green tea extracts showed significant antimicrobial activity against Micrococcus luteus (M. luteus), once fractionated, the ethyl acetate fraction did not show any antimicrobial activity against M. luteus. MICs of the ethyl acetate fraction were $5\;\;{\mu}L$/disc against B. subtilis and $3\;{\mu}L$/disc against S. aureus, S. mutans and V. parahaemolyticus. 90% inhibition of B. subtilis was observed with 0.05% ethyl acetate fraction but S. mutans needed over 0.1% ethyl acetate fraction to exhibit the same inhibition as B. subtilis. Antimicrobial activities of ethyl acetate fractions were reduced around 10% by thermal treatment at $121^{\circ}C$ for 20 min. All the results suggest that the 70% ethanol extract as well as the ethyl acetate fraction from green tea used for coarse tea could be further developed into a natural antimicrobial agent.

Antioxidative Effects of Campanula takesimana Nakai Extract (섬초롱(Campanula takesimana Nakai) 용매 추출물의 항산화 효과)

  • Kim, Mi-Seon;Kim, Kyoung-Hee;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.10
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    • pp.1331-1337
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    • 2012
  • The study analyzed the total polyphenolic contents, DPPH radical scavenging activities, ABTS radical scavenging activities, reducing power levels, SOD-like activities, and tyrosinase inhibition activities of fractions from Campanula takesimana Nakai extract. Total polyphenolic content of the ethyl acetate fraction from Campanula takesimana Nakai extract was the highest. The ethyl acetate fraction of Campanula takesimana Nakai extract showed the lowest $IC_{50}$ value (0.13 mg/mL) as well as greater than 98% ABTS radical scavenging activity, which was similar to ascorbic acid. Further, reducing power was significantly higher in the ethyl acetate fraction. The SOD-like activity of edible plant was 70.56% (n-butanol fraction) and 62.27% (ethyl acetate fraction) at 1 mg/mL. Tyrosinase inhibition activity of Campanula takesimana Nakai extract was reduced to 34.77% in the n-butanol fraction and 30.85% in the ethyl acetate fraction at 5 mg/mL. These results suggest that the ethyl acetate and n-butanol fractions of Campanula takesimana Nakai extract could be applicable as food additives or cosmeceutical ingredients.

Antihyperlipidemic Activity of the Ethyl-acetate Fraction of Stereospermum Suaveolens in Streptozotocin-induced Diabetic Rats

  • Thirumalaisamy, Balasubramanian;Prabhakaran, Senthilkumar Gnanavadevel;Marimuthu, Karthikeyan;Chatterjee, Tapan Kumar
    • Journal of Pharmacopuncture
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    • v.16 no.3
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    • pp.23-29
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    • 2013
  • Objectives: Dyslipidemia in diabetes mellitus is a significant risk factor for the development of cardiovascular complications. The aim of this study was to evaluate the effect of the ethyl-acetate fraction of an ethanolic extract from Streospermum suaveolens on lipid metabolism in streptozotocin (STZ)-induced diabetic rats. Methods: Diabetes was induced by intraperitonial injection of STZ (50 mg/kg). Diabetic rats were treated with an ethyl-acetate fraction orally at doses of 200 and 400 mg/kg daily for 14 days. On the $15^{th}$ day, serum lipid profiles, such as total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), were estimated in experimental rats. The atherogenic (AI) and the coronary risk (CRI) indices were also evaluated. Results: The ethyl-acetate fraction at doses of 200 and 400 mg/kg significantly (P < 0.001) and dose-dependently reduced serum cholesterol, triglycerides and LDL, but increased HDL towards near normal levels as compared to diabetic control rats. The fraction also significantly (P < 0.001) lowered the atherogenic index (AI) and coronary risk index (CAI) in a dose-dependent manner. Conclusion: The present study demonstrated that the ethyl-acetate fraction of Stereospermum suaveolens exhibits a potent antihyperlipidemic activity in hyperglycemic rats and suggests that the plant may have therapeutic value in treating the diabetic complication of hyperlipidemia.

Antioxidative Activity of Ethanol Extract from Lotus (Nelumbo nucifera) Leaf (연잎 에탄올 추출물의 항산화 효과)

  • Lee, Kyung-Seok;Kim, Min-Gue;Lee, Ki-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.2
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    • pp.182-186
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    • 2006
  • This study was investigated on the antioxidative activity of $70\%$ ethanol extracts from lotus (Nelumbo nucifera) leaf. The extraction yields of $70\%$ ethanol extract was $8.16\%$. The extract was further fractionated subsequently by hexane, chloroform, ethyl acetate, butanol and water. Antioxidative activity was examined by electron donating ability (EDA) using DPPH, TBA value, acid value (AV), and peroxide value (POV), in comparison with commercial antioxidants. Butanol fraction showed the highest extraction yields but ethyl acetate fraction showed the highest phenol content. The ethyl acetate fraction showed the highest EDA. The antioxidative activity of ethyl acetate fraction determined by AV and POV in accelerated oxidation condition of lard was similar to that of BHA.