• Title, Summary, Keyword: erythrosine

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Interaction of Erythrosine with Several Pharmaceutical Gelatins (Erythrosine과 수종(數種) 젤라틴간의 상호작용(相互作用))

  • Min, Shin-Hong;Kwon, Jong-Won;Jheong, Yeoub
    • Journal of Pharmaceutical Investigation
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    • v.14 no.4
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    • pp.183-188
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    • 1984
  • The interactions of FD & C Red No.3 (erythrosine) in 1 or 2% concentrations with various types of pharmaceutical gelatins were studied. In visible region spectroscopy type A gelatin showed 6 nm bathochromic shift in ${\lambda}_{max}$ of erythrosine, but no shifts were observed in other gelatins. Various results were obtained in the decrease of absorbance from 0.06 to 0.25. In dissolution study, the gelatin that showed the greatest spectral change exhibited the worst profile. From above results, it is concluded that erythrosine interacted to the greatest extent with type A gelatin and in the case of type B gelatin the degree of the interaction in different according to their specifications.

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Postantibiotic Effects of Photodynamic Therapy Using Erythrosine and Light Emitting Diode on Streptococcus mutans

  • Yoo, Min Seok;Lee, Si Young
    • International Journal of Oral Biology
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    • v.41 no.1
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    • pp.39-43
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    • 2016
  • Dental caries, the most common oral disease, is a multifactorial disease caused by interactions among bacteria within the dental plaque, food, and saliva, resulting in tooth destruction. Streptococcus mutans has been strongly implicated as the causative organism in dental caries and is frequently isolated from human dental plaque. Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. Postantibiotic effect (PAE) is defined as the duration of suppressed bacterial growth following brief exposure to an antibiotic. In this study, the in vitro PAE of PDT using erythrosine and light emitting diode on S. mutans ATCC 25175 was investigated. The PAE of PDT for 1 s irradiation and 3 s irradiation were 1.65 h and 2.1 h, respectively. The present study thus confirmed PAE of PDT using erythrosine on S. mutans.

The effect of photodynamic therapy on Aggregatibacter actinomycetemcomitans attached to surface-modified titanium

  • Cho, Kyungwon;Lee, Si Young;Chang, Beom-Seok;Um, Heung-Sik;Lee, Jae-Kwan
    • Journal of Periodontal and Implant Science
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    • v.45 no.2
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    • pp.38-45
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    • 2015
  • Purpose: The purpose of this study was to evaluate the effect of photodynamic therapy (PDT) using erythrosine and a green light emitting diode (LED) light source on biofilms of Aggregatibacter actinomycetemcomitans attached to resorbable blasted media (RBM) and sandblasted, large-grit, acid-etched (SLA) titanium surfaces in vitro. Methods: RBM and SLA disks were subdivided into four groups, including one control group and three test groups (referred to as E0, E30, E60), in order to evaluate the effect of PDT on each surface. The E0 group was put into $500{\mu}L$ of $20{\mu}M$ erythrosine for 60 seconds without irradiation, the E30 group was put into erythrosine for 60 seconds and was then irradiated with a LED for 30 seconds, and the E60 group was put into erythrosine for 60 seconds and then irradiated with a LED for 60 seconds. After PDT, sonication was performed in order to detach the bacteria, the plates were incubated under anaerobic conditions on brucella blood agar plates for 72 hours at $37^{\circ}C$, and the number of colony-forming units (CFUs) was determined. Results: Significant differences were found between the control group and the E30 and E60 groups (P<0.05). A significantly lower quantity of CFU/mL was found in the E30 and E60 groups on both titanium disk surfaces. In confocal scanning laser microscopy images, increased bacterial death was observed when disks were irradiated for a longer period of time. Conclusions: These findings suggest that PDT using erythrosine and a green LED is effective in reducing the viability of A. actinomycetemcomitans attached to surface-modified titanium in vitro.

Susceptibility of Mutans streptococci in the Planktonic and Biofilm State to Erythrosine (부유 상태와 바이오필름 상태에서 Mutans streptococci의 Erythrosine에 대한 감수성 평가)

  • Gong, Jungeun;Seo, Hyunwoo;Lee, Siyoung;Park, Howon;Lee, Juhyun
    • THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY
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    • v.46 no.2
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    • pp.135-138
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    • 2019
  • The aim of this study was to investigate the susceptibility of Mutans streptococci in both planktonic and biofilm states to erythrosine. S. mutans was cultured in brain-heart infusion (BHI) broth. Erythrosine was diluted in BHI broth and prepared at a concentration range of $0.02-10000{\mu}g/L$. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured using the microdilution method. After forming biofilms on 96-well plates, the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were measured. S. mutans was susceptible to erythrosine in both planktonic and biofilm states. MIC and MBC values were both $19.5{\mu}g/L$ for the planktonic state, while MBIC and MBEC values were $313{\mu}g/L$ and $2500{\mu}g/L$, respectively, for the biofilm state. Erythrosine ($19.5{\mu}g/L$) exhibited a bactericidal effect on S. mutans (killing 99.9%) in the planktonic state. For biofilms, erythrosine inhibited biofilm growth and eradicated 99.9% of biofilm bacteria at higher concentrations than MIC and MBC. These MBIC and MBEC concentrations are much lower than known noxious doses, and the MIC, MBC, and MBIC values were even lower than clinical concentrations.

Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent (치면착색제를 이용한 광역동 치료의 항균 효과)

  • Kho, Junhee;Park, Howon;Lee, Juhyun;Seo, Hyunwoo;Lee, Siyoung
    • THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY
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    • v.47 no.2
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    • pp.120-127
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    • 2020
  • The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans, Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.

THE EFFECT OF PHOTODYNAMIC THERAPY ON THE VIABILITY OF STREPTOCOCCUS MUTANS ISOLATED FROM ORAL CAVITY (광역동 치료가 구강 내에서 분리한 수종의 Streptococcus mutans의 생존력에 미치는 영향)

  • Jung, Ji-Sook;Park, Ho-Won;Lee, Ju-Hyun;Seo, Hyun-Woo;Lee, Si-Young
    • THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY
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    • v.39 no.3
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    • pp.233-241
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    • 2012
  • Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizer by light in the presence of tissue oxygen, resulting in the production of reactive radicals capable of inducing cell death. The aim of this study was to evaluate the effect of PDT on Streptococcus mutans in planktonic conditions, previously treated with different photosensitive concentrations of erythrosine, using halogen and LED curing unit as a light source. And we compared the effects of PDT on six strains of S. mutans isolated from oral cavity and reference strain. As a result, S. mutans was susceptible to the combination of hand held photopolymerizer (HHP) and erythrosine. The higher concentration of erythrosine in the presence of light irradiation induced greater effects in reduction of viability of S. mutans. Isolated S. mutans showed a significant reduction in bacterial counts of the groups submitted to PDT compared to the control groups. And they appeared to be similar or slightly lower antimicrobial effect compared with reference strain. However, the difference was not significant (p < 0.05). In conclusion, PDT using erythrosine as a photosensitizing agent and HHP as a light source could be an efficient option for diseases caused by S. mutans.

Studies on the interaction of edible dyes with protein I

  • Jang, Seong-Ki;Kim, Bak-Kwang;Lee, Wang-Kyu
    • Archives of Pharmacal Research
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    • v.8 no.3
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    • pp.169-175
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    • 1985
  • The binding of bovine serum albumin (BSA)-edible dyes was studied by spectrophotometric method. The edible dyes used in this study were amaranth, erythrosine, tartrazine and sunset yellow. The binding free energies and binding sites were determined at pH 7.4. The ranges of edible dye concentration were from 0.3 to $7{\times}10^{-5}$M, and those of BSA were from 0.15 to $3{\times}10^{-5}$M. The binding free energies of BSA-edible dyes were from -6, 300 to -8, 100 cal/mole.

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Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach (치아우식 예방을 위한 치아미백기의 활용 : 광역동 치료로서의 접근)

  • Park, Choa;Park, Howon;Lee, Juhyun;Seo, Hyunwoo;Lee, Siyoung
    • THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY
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    • v.47 no.1
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    • pp.70-77
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    • 2020
  • The present study is aimed to assess the effect of antimicrobial photodynamic therapy (aPDT) on Streptococcus mutans biofilm through teeth whitening light emitting diode (LED). Planktonic and dynamic biofilm state cultures of S. mutans were used. Erythrosine 20 μM/L was used as the photosensitizer. Irradiation was performed by exposing cultures to clinic and homecare whitening LEDs for 15 minutes. The viability was measured through Colony Forming Unit counts and confocal laser scanning microscopy. aPDT using whitening LEDs and erythrosine significantly decreased the CFU count of S. mutans compared to that in the control group. Dynamic biofilm group showed more resistant features to aPDT compared with planktonic state. Clinic and homecare whitening LED device showed similar antimicrobial effect. The whitening LED, which could irradiate the entire oral arch, showed a significant photodynamic effect on cariogenic S. mutans biofilm. aPDT mediated by erythrosine and LEDs used for teeth whitening exhibited promising antimicrobial activity.

A Study on the Effects of the Dissociation Constant of Edible Tar Pigment. I (혼합용매중(混合溶媒中)에서의 식용(食用) Tar색소(色素)의 해리정수(解離定數)에 관(關)한 연구(硏究) (제 1 보)(第 1 報) -2',4',5',7'- Tetraiodofluorescein Sodium염(鹽)에 대(對)하여-)

  • Kim, Bak-Kwang;Lee, Min-Hwa;Cho, Young-Mee;Kim, Kil-Su;Moon, Dong-Chul
    • Journal of Pharmaceutical Investigation
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    • v.10 no.3
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    • pp.14-19
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    • 1980
  • The dissociation constant of erythrosine in the mixed solvent systems of water-alcohols and water-acetone at various pH and in concentration media were determined by spectrophotometric method. Methanol, ethanol, and acetone were employed as solvents and studied the changes of pK values of erythrosine according to the kind and change of concentration of organic solvent which is mixed into water solution. The effect of organic solvent on pK is negligible at 40% while in the case of above 40% mixing, it is not negligible.

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Studies on the Interaction of Edible Dyes with Protein (II). The effects of drug additions on protein binding of edible dyes

  • Kim, Bak-Kwang;Lah, Woon-Lyong;Jang, Seong-Ki;Lim, Bang-Ho;Jang, Jae-Yeon;Lee, Wang-Kyu
    • Archives of Pharmacal Research
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    • v.10 no.1
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    • pp.29-35
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    • 1987
  • The effect of drug addition on the bovine serum albumin (BSA)-edible dye complex was studied by spectrophotometric method. The edible dyes tested were amranth, erythrosine, tatrazine and sunset yellow. The moles of bound dye per protein mole and free energies for edible dyes bounded were determined at pH 7.4. The values of free energy change by the addition of drughs to BSA-edible dye were ranged fro -6, 260 to 08030 cal/mole. In the wide range of edible dye concentration (0.3-$7{\times}10^{-5}$$^{-5}$ M), acetylsalicylic acid (ASA) showed pattern of displacement different from that of dye. It was assumed that ASA has different binding mechanisms from edible dye.

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