• Title/Summary/Keyword: enzymatic hydrolysis

Search Result 475, Processing Time 0.369 seconds

Effects of Enzymatic Hydrolysis and Concentrations of Sugar and Salt on Kimchi Juice Fermentation of Outer Leaves of Chinese Cabbage (배추 겉잎을 이용한 김치쥬스 제조시 효소분해, 당, 소금농도가 발효에 미치는 영향)

  • 전윤기;윤석권;김우정
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.26 no.5
    • /
    • pp.788-793
    • /
    • 1997
  • Addition of sugar, enzymatic hydrolysis and salt concentration were evaluated for their effects on the changes in some characteristics of Kimchi juice during fermentation. The Kimchi juice was prepared by brining and grinding of outer layer leaves of chinese cabbage, one of the wastes products of Kimchi processing, followed by fermentation proceeded significantly faster. Addition of sucrose or glucose at the ange of 0.5~2.0 % also improved the fermentation but the concentration effect was little. Enzymatic hydrolysis on the brined cabbage prior to fermentation with a commercial polysaccharides hydrolases also increased the fermentation. However the solid concentration in Kimchi juice was rather decreased by higher concentration of NaCl and enzymatic hydrolysis. The reducing sugar content showed a rapid decrease from 24 hours of fermentation and the effect of enzymatic hydrolysis was little.

  • PDF

Studies on the Enzymatic Hydrolysis of Lignocellulosic Materials for the Alternative Fuels (II) - The Effect of Delignification Treatment on the Enzymatic Hydrolysis of Steam - Exploded Woods - (대체연료(代替燃料) 생산(生産)을 위한 목질재료(木質材料)의 가수분해(加水分解)에 관한 연구(硏究)(II) - 탈(脫)리그닌처리가 폭쇄처리재(爆碎處理材)의 효소적(酵素的) 당화(糖化)에 미치는 영향(影響) -)

  • Cho, Nam-Seok
    • Journal of the Korean Wood Science and Technology
    • /
    • v.18 no.4
    • /
    • pp.18-25
    • /
    • 1990
  • As polysaccharides in lignocellulosic materials are encrusted with aromatic lignin molecules and have high crystallinity, these require pretreatment to improve their digestability by cellulolytic enzymes. Though a number of pretreatment methods have been proposed, the steam explosion process is evaluated as a promising method. This study was performed to investigate the effect of delignification treatment by alkali, methanol and the others on the enzymatic hydrolysis. Delignification treatment resulted in great increase rate in enzymatic hydrolysis. Concerning to the effect of delignication reagents on the enzymatic hydrolysis, methanol treatment was more effective than alkali in the case of oak wood. In pine wood, the delignification did not showed any significant enhancement of hydrolysis rate. Complete delignification by Alkali-Oxygen. Alkali treatment showed high saccharification rate of 99.5%.

  • PDF

Optimization of organosolv pretreatment with sulfuric acid for enhancing enzymatic hydrolysis of Pitch Pine (Pinus rigida)

  • Park, Na-Hyun;Kim, Hye-Yun;Gwak, Ki-Seob;Koo, Bon-Wook;Yeo, Hwan-Myeong;Choi, In-Gyu
    • 한국신재생에너지학회:학술대회논문집
    • /
    • /
    • pp.505-505
    • /
    • 2009
  • The object is to optimize the best condition of organosolv pretreatment process with sulfuric acid as a catalyst. As a material, Pitch pine (Pinus rigida) was ground and sieved through 40-mesh screen, and Celluclast and $\beta$-glucosidase were used as enzymes for enzymatic hydrolysis. Pretreatment processes were carried out in the minibomb, and 20 g of materials with 200 ml of 50% ethanol solution (v/v) with 1% sulfuric acid as a catalyst. Pretreatment temperature was varied from $150^{\circ}C$ to $190^{\circ}C$, and time was varied from 0 to 20 min. Then, residual materials were used for enzymatic hydrolysis. The best conditions were selected by estimating followed enzymatic hydrolysis rate and degradable rates after pretreatment process. The highest value of enzymatic hydrolysis rate was obtained as 55 - 60% at 160 and at $180^{\circ}C$, but the value decreased under more severe conditions. As the residual rates decreased under severe conditions, it infered that the decrease of sugar contents limits enzymatic hydrolysis rates. Combined with enzymatic hydrolysis rate, degradable rates and H-factors, the temperatures at $160^{\circ}C$ for 20 min and at $180^{\circ}C$ for 0 min were concluded as the optimized conditions where have the lowest H-factor value for considering energy input.

  • PDF

Enzymatic hydrolysis and micro-structure of ozone treated wood meal (오존 처리에 의한 목재 세포벽의 미세구조변화와 효소가수분해)

  • Kim, Kang-Jae;Eom, Tae-Jin
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.42 no.3
    • /
    • pp.67-73
    • /
    • 2010
  • Pine (Pinus densiflora) and aspen (Populus euramericana) wood meals were treated with ozone at various time schedule in acidic condition. The lignin contents and surface area of the ozone treated wood meals were determined and the enzymatic hydrolysis rate of ozonated wood meals was evaluated. The feasibility of enzymatic hydrolysis of the ozone treated wood meal was obviously influenced with the degree of delignification. After ozone treatment of wood meal for 10min, total pore volume were slightly increased in the surface of wood meal. When wood meals were treated with ozone longer than 10min, few change in the pore volume was observed. However, the area of over $50{\AA}$ of pore size is increased with ozonation time. As a conclusion, the rate of enzymatic hydrolysis of wood is more effective with the pore size distribution than the total pore volume.

Characteristics of Enzymatic Hydrolysis of Sodium Hydroxide pretreated Suwon Poplar (NaOH 전처리된 현사시나무의 효소가수분해 특성)

  • 박영기;오정수
    • Journal of Korea Foresty Energy
    • /
    • v.20 no.2
    • /
    • pp.20-27
    • /
    • 2001
  • An effective method for production of glucose was developed using enzymatic hydrolysis of Suwon poplar by the cellulase. Enzymatic hydrolysis of wood is the reaction to produce glucose from wood using enzyme which derives from microorganism. Glucose can be transferred easily to ethanol by fermentation. Ethanol is the starting material for producing acetone, butanol, citric acid and lactic acid. The mechanism of the enzymatic hydrolysis of cellulose are reasonably explained in terms of the sequential action of three different types of enzymes, endo-cellulase, ex-cellulase, and $\beta$ -glucosidase. The goal of this work was to investigate the cellulose hydrolysis pretreated polar with various concentration NaOH, the crystallinity of cellulose, lignin contents and the degree of hydrolysis.

  • PDF

A Research Trend of Enzymatic Hydrolysis of Lignocellulosic Biomass : A Literature Review (목질바이오매스의 효소 당화 기술에 관한 연구 동향)

  • Kim, Yeong-Suk
    • Journal of Forest and Environmental Science
    • /
    • v.26 no.2
    • /
    • pp.137-148
    • /
    • 2010
  • The high costs for ethanol production with lignocellulosic biomass as a second generation energy materials currently deter commercialization of lignocellulosic biomass, especially wood biomass which is considered as the most recalcitrant material for enzymatic hydrolysis mainly due to the high lignified structure and the nature of the lignin component. Therefore, overcoming recalcitrance of lignocellulosic biomass for converting carbohydrates into sugar that can subsequently be converted into biobased fuels and biobased products is the primary technical and economic challenge for bioconversion process. This study was mainly reviewed on the research trend of the enhancement of enzymatic hydrolysis for lignocellulosic biomass after pretreatment in bioethanol production process.

Hydrolysis of Pulp Sludge for Lactic Acid Fermentation using Enzyme System

  • Lee, Sang-Mok;Jianqiang, Lin;Gu, Yun-Mo
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.504-507
    • /
    • 2000
  • Enzymatic hydrolysis of cellulose was studied with emphasis on the effect of cellulase loading and pulp sludge concentration on glucose yield. Enzyme loading appeared to have a significant effect on glucose yield. Chemical pretreatment had no effect on enzymatic hydrolysis of pulp sludge. High glucose yield was obtained from enzymatic hydrolysis, especially at sludge concentrations lower than twenty percent. The optimum concentrations of crude cellulase and ${\beta}-glucosidase$ were 5 U/mL and 8 U/mL, respectively, considering the amount of enzymes used and glucose produced.

  • PDF

Optimum Reaction Condition of Enzymatic Hydrolysis for Production of Reducing Sugar from Enteromorpha intestinalis (창자파래로부터 환원당 생산을 위한 효소가수분해의 최적 반응조건)

  • Kim, A-Ram;Kim, Dong-Hyun;Jeong, Gwi-Taek
    • KSBB Journal
    • /
    • v.30 no.2
    • /
    • pp.53-57
    • /
    • 2015
  • In this study, the production of total reducing sugar from macro green-algae Enteromorpha intestinalis by enzymatic hydrolysis was investigated. As a result of enzymatic hydrolysis using 13 kind commercial enzymes, the highest yield of 8.75% was obtained from Viscozyme L, which is multi-enzyme complex such as cellulase, arabanase, beta-glucanase, hemicellulase and xylanase. As a control, only 0.33% and 0.27% yield were obtained from 1% sulfuric acid and 0.05 M citrate buffer (pH 4.8), respectively. In the case of enzyme mixture, the mixture of $Viscozyme^{(R)}$ L and $Cellic^{(R)}$ CTec2 (1:1) was presented the highest yield of 10.67%. Finally, the 14.99% yield was obtained at 36 hr under the condition of 10% biomass and 30% enzyme mixture.

Study on the Change of Antioxidant Activity by Enzymatic Hydrolysis in Sophora japonica Linne, Houttuynia cordata Thunberg, Leonurus japonicus Houttuyn (괴화, 어성초, 익모초에서 효소 분해에 의한 항산화 활성 변화 연구)

  • Cha, Bae Cheon
    • Journal of Korean Medicine for Obesity Research
    • /
    • v.21 no.1
    • /
    • pp.1-9
    • /
    • 2021
  • Objectives: The enzymatic hydrolysis is one of the processing methods that improve its effectiveness on medicinal herbs. In this research, changes in ingredients and activity by enzymatic hydrolysis were studied. Methods: For this study, a carbohydrate hydrolase such as viscozyme, which converts glycosides to aglycone, was applied to induce constituent changes in Sophora japonica Linne, Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn. Changes in antioxidant activity were measured using the 1,1-diphenyl-2-picrylhydrazl (DPPH) method, and changes in ingredients were analyzed by high performance liquid chromatography. Results: As a result of enzymatic hydrolysis, the content of quercetin was increased from 1.26 mg/g to 29.66 mg/g in Sophora japonica Linne, from 0 mg/g to 0.66 mg/g in Houttuynia cordata Thunberg and from 0.43 mg/g to 0.71 mg/g in Leonurus japonicus Houttuyn. As a result of the antioxidant experimentation, the IC50 of Sophora japonica Linne decreased from 5 ug/ml (MeOH extract) and 9.1 ug/ml (EtOAc fraction) to 3.0 ug/ml, Houttuynia cordata Thunberg decreased from 15.6 ug/ml (MeOH extract) and 13.6 ug/ml (EtOAc fraction) to 11.2 ug/ml, and Leonurus japonicus Houttuyn decreased from 14.4 ug/ml (MeOH extract) and 12.6 ug/ml (EtOAc fraction) to 10.2 ug/ml. Conclusion: In conclusion, it was confirmed that glycoside rutin contained in the three medicinal herbs was changed to quercetin which is the aglycone, by the enzymatic hydrolysis using viscozyme. In terms of antioxidant activity, Sophora japonica Linne showed a significant antioxidant activity value that closes to the control group butylated hydroxyanisole. Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn showed a minor increase in antioxidant activity.

Enzymatic Hydrolysis of Egg Yolk Protein in Continuous Packed Column Operation

  • Kang, Byung-Chul;Lee, Sang-Uk
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.485-489
    • /
    • 2003
  • Enzymatic hydrolysis of egg yolk Protein was carried out in continuous packed column reactor Five supports for enzyme immobilization were evaluated in this study. We investigated the optimum operation variables - pH, temperature, and flow rate in continuous reactor operation.

  • PDF