• Title, Summary, Keyword: differentiation

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The Effects of Intergenerational Transmission of Self-Differentiation on Psychological Adjustment of College Student (자기분화의 세대간 전이가 대학생의 심리적 적응에 미치는 영향)

  • Ha, Sang-Hee;Chung, Hye-Jeong
    • Journal of the Korean Home Economics Association
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    • v.46 no.7
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    • pp.95-108
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    • 2008
  • The major purpose of this study was to investigate the effect of intergenerational transmission of self-differentiation on psychological adjustment of college students. Specifically, the present study endeavored to test the causal model focusing on the effect of the father's and the mother's self-differentiation on college student's psychological adjustment through the student's self-differentiation. The data were collected from 254 male and 293 female college students and their parents by using self-administered questionnaire method. The casual model was tested through structural equation model by using AMOS 7.0 program. The results showed that mother's and father's self-differentiation had direct and indirect effect on student's psychological adjustment. The results showed that both mother's and father's self-differentiation had direct effect on son's self-differentiation level, which directly influenced his psychological adjustment. The test of structural equation model also revealed that daughter's self-differentiation level was positively influenced only by mother's self-differentiation, while father's self-differentiation did not influenced female student's self-differentiation. Both male and female student's psychological adjustment was directly influenced by his or her self-differentiation level.

miRNA-222 Modulates Differentiation of Mouse Embryonic Stem Cells

  • Ahn, Hee-Jin;Jung, Jee-Eun;Park, Kyung-Soon
    • Development and Reproduction
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    • v.15 no.4
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    • pp.331-338
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    • 2011
  • MicroRNAs (miRNAs) function as a key regulator of diverse cellular functions. To find out novel miRNAs that promote the differentiation of mouse embryonic stem cells (mESCs), we compared the miRNAs expression profiles of mESCs under self-renewal vs. differentiation states. We noticed that miR-222 was highly expressed during the differentiation of mESCs. Quantitative RT-PCR analysis revealed that expression of miR-222 was up-regulated during the embryonic bodies formation and retinoic acid -dependent differentiation. When miR-222 was suppressed by antogomiR-222, the differentiation of mESCs was delayed compared to control. Self-renewal marker expression or cell proliferation was not affected but the expression of lineage specific marker was suppressed by the treatment of miR-222 inhibitor during the differentiation of mESCs. Taken together, these results suggest that miR-222 functions to promote the differentiation of mESCs by regulating expression of differentiation related genes.

Identification of anti-adipogenic proteins in adult bovine serum suppressing 3T3-L1 preadipocyte differentiation

  • Park, Jeongho;Park, Jihyun;Nahm, Sang-Soep;Choi, Inho;Kim, Jihoe
    • BMB Reports
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    • v.46 no.12
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    • pp.582-587
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    • 2013
  • Adipocyte differentiation is a complex developmental process forming adipocytes from various precursor cells. The murine 3T3-L1 preadipocyte cell line has been most frequently used in the studies of adipocyte differentiation. Differentiation of 3T3-L1 preadipocytes includes a medium containing fetal bovine serum (FBS) with hormonal induction. In this study, we observed that differentiation medium containing adult bovine serum (ABS) instead of FBS did not support differentiation of preadipocytes. Impaired adipocyte differentiation was due to the presence of a serum protein factor in ABS that suppresses differentiation of preadipocytes. Using a proteomic analysis, alpha-2-macroglobulin and paraoxonase/arylesterase 1, which were previously shown to suppress differentiation of preadipocytes, were identified as anti-adipogenic proteins. Although their functional mechanisms have not yet been elucidated, the anti-adipogenic effects of these proteins are discussed.

Effect of Polyamines on Cellular Differentiation of N. gruberi: Inhibition of Translation of Tubulin mRNA

  • Yoo, Jin-Uk;Kwon, Kyung-Soon;Cho, Hyun-Il;Kim, Dae-Myung;Chung, In-Kwon;Kim, Young-Min;Lee, Tae-Ho;Lee, Joo-Hun
    • Journal of Microbiology
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    • v.35 no.4
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    • pp.315-322
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    • 1997
  • The effects of a polyamine, spermine, on the differentiation of Naegleria gruberi amebas into flagellates were tested. Addition of spermine at early stages of differentiation (until 40 min after the initiation of differentiation) completely inhibited the differentiation. To understand the inhibition mechanism, we examined the effect of spermine treatment on the transcription and translation of differentiation-specific genes during differentiation. Addition of spermine at early stages did not inhibit the accumulation of two differentiation-specific mRNAs, ${\alpha}$-tubulin and Class I mRNA, significantly, but rather prevented the rapid degradation of the mRNAs in later overall protein synthesis partially and gradually. However, translation of the ${\alpha}$-tubulin mRNA was completely inhibited. These data suggest that the inhibition of differentiation of N. gruberi by spermine treatment did not result from the inhibition of transcription of differentiation-specific genes but from the specific inhibition of translation of the mRNAs during the differentiation.

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Comparative Review of Treatment Based on Syndrome Differentiation and Treatment Based on Syndrome Differentiation from Disease - Emphasis on Hypertension and Headache - (변증논치(辨證論治)와 병증변치(病證辨治) 장단점의 비교고찰 - 고혈압과 두통의 예를 중심으로 -)

  • Gi, Youjong;Shin, Sunjoong;Han, Wonyoung;Kim, Hyundo;Han, Yoochang;Lee, Sundong
    • Journal of Society of Preventive Korean Medicine
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    • v.21 no.1
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    • pp.9-17
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    • 2017
  • Objectives : Comparative review was rendered to evaluate strengths and weaknesses of two common treatment approaches of treatment based on syndrome differentiation and treatment based on syndrome differentiation from disease. A typical symptom of headache from hypertension was chosen for substantial approach of this review. Methods : Pros and cons of two different approaches to disease were evaluated based on literatures and texts focusing treatment based on syndrome differentiation and treatment based on syndrome differentiation from disease. Headache can be correlated with hypertension yet it can be induced by multiple other factors, and headache may/may not accompany hypertension. Hypertension is an example of treatment based on syndrome differentiation from disease and headache can be an example of treatment based on syndrome differentiation. Results : Treatment based on syndrome differentiation can give more autonomy and flexibility in approach to the disease, Intuition, experience, and traditional medical theory can be applied with ease. However, management and eradication of diseases are difficult and standardization of treatment is not easy among practitioners. Treatment based on syndrome differentiation from disease incorporated merits of both eastern and western medicines, achieving more evidence based diagnosis and treatment. Eradication and standardization of disorders are possible with the latter approach. Conclusions : Comparing two systems of treatment based on syndrome differentiation and treatment based on syndrome differentiation from disease with emphasis on hypertension and headache yielded medical values. Treatment based on syndrome differentiation from disease appears to be superior in medical values and effectiveness, but further evaluation and interest are needed to make advancement in Korean traditional medicine.

Relationship of Vertical versus Horizontal Individualism and Collectivism with Self-differentiation among Korean College Students

  • Chung, Hye-Jeong
    • International Journal of Human Ecology
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    • v.10 no.1
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    • pp.47-59
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    • 2009
  • This study investigates the relationship of self-differentiation with individualism and collectivism among college students. Special focus was on whether distinctions between vertical and horizontal individualism and collectivism were more useful in examining the relationship with self-differentiation. The results reveal that different aspects of individualism and collectivism were differently related to self-differentiation, indicating that self-differentiation was negatively associated with all aspects of collectivism and with vertical individualism, but positively with horizontal individualism. This study proved that four distinct constructs were more useful in explaining the effects on self-differentiation. The implications of the findings are discussed for clinicians and future research.

The IRF2BP2-KLF2 axis regulates osteoclast and osteoblast differentiation

  • Kim, Inyoung;Kim, Jung Ha;Kim, Kabsun;Seong, Semun;Kim, Nacksung
    • BMB Reports
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    • v.52 no.7
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    • pp.469-474
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    • 2019
  • Kruppel-like factor 2 (KLF2) has been implicated in the regulation of cell proliferation, differentiation, and survival in a variety of cells. Recently, it has been reported that KLF2 regulates the p65-mediated transactivation of $NF-{\kappa}B$. Although the $NF-{\kappa}B$ pathway plays an important role in the differentiation of osteoclasts and osteoblasts, the role of KLF2 in these bone cells has not yet been fully elucidated. In this study, we demonstrated that KLF2 regulates osteoclast and osteoblast differentiation. The overexpression of KLF2 in osteoclast precursor cells inhibited osteoclast differentiation by downregulating c-Fos, NFATc1, and TRAP expression, while KLF2 overexpression in osteoblasts enhanced osteoblast differentiation and function by upregulating Runx2, ALP, and BSP expression. Conversely, the downregulation of KLF2 with KLF2-specific siRNA increased osteoclast differentiation and inhibited osteoblast differentiation. Moreover, the overexpression of interferon regulatory protein 2-binding protein 2 (IRF2BP2), a regulator of KLF2, suppressed osteoclast differentiation and enhanced osteoblast differentiation and function. These effects were reversed by downregulating KLF2. Collectively, our data provide new insights and evidence to suggest that the IRF2BP2/KLF2 axis mediates osteoclast and osteoblast differentiation, thereby affecting bone homeostasis.

Regulation of Actin Gene Expression During the Differentiation of Naegleria gruberi

  • Kim, Misook;Lee, Joo-Hun
    • Journal of Microbiology
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    • v.39 no.1
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    • pp.42-48
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    • 2001
  • The regulation of actin gene expression during the differentiation of Naegleria gruberi was examined. Actin mRNA concentration was maximal in amoebae and decreased rapidly after the initiation of differentiation. At 20 min after initiation, the concentration of actin mRNA decreased to 55% of the maximal value. The actin mRNA concentration decreased to the minimum at 80 min (15% of the maximum), and then began to increase slightly at the end of differentiation. This decrease of actin mRNA concentration was regulated by the repression of actin gene transcription based on nuclear run-on transcription experiments. The rates of transcription of actin gene in nuclei prepared at 40 and 80 min after the initiation of differentiation were 50 and 28% of that of nuclei prepared at the beginning of differentiation, respectively. The addition of cycloheximide at the initiation of differentiation inhibited both the rapid decrease in the concentration of actin mRNA and the repression of actin gene transcription. These results suggest that the rapid decrease in the concentration of actin mRNA during the differentiation of N. gruberi is accomplished by the repression of actin gene transcription and this transcriptional regulation requires continuous protein synthesis during the differentiation.

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Price Competition in Horizontal and Vertical Differentiation : Focusing on the WiBro and HSDPA (수직적.수평적 차별화 시장에서의 서비스 요금전략 : 와이브로와 HSDPA 서비스를 중심으로)

  • Kim, Dow-Han
    • Korean Management Science Review
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    • v.26 no.3
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    • pp.67-78
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    • 2009
  • In this paper, I analyze the mobile broadband services market characterized by vertical and horizontal differentiation. Vertical differentiation as service quality differentiation is based on the transmission speed of mobile internet service and horizontal differentiation as spatial differentiation is based on the service coverage. Theoretical explanations for the competitive price policy have been developed in the game context of WiBro which represent the high quality within the limited service coverage and HSDPA which represent relatively low quality with nation-wide service. When the WiBro has a mobile broadband service quality advantage and the difference in quality is sufficiently low, the price of WiBro with limited service coverage is relatively lower than that of HSDPA. This occurs because the advantage of WiBro's vertical service differentiation is offset by the disadvantage of horizontal differentiation. The difference in the quality of mobile internet service, however, is not too high, the price of WiBro is relatively higher than that of HSDPA. Moreover, when the service quality of WiBro is sufficiently high, the low quality HSDPA service faces no demand.

Detection of Neural Fates from Random Differentiation : Application of Support Vector MachineMin

  • Lee, Min-Su;Ahn, Jeong-Hyuck;Park, Woong-Yang
    • Genomics & Informatics
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    • v.5 no.1
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    • pp.1-5
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    • 2007
  • Embryonic stem cells can be differentiated into various types of cells, requiring a tight regulation of transcription. Biomarkers related to each lineage of cells are used to guide the differentiation into neural or any other fates. In previous experiments, we reported the guided differentiation (GD)-specific genes by comparing profiles of random differentiation (RD). Interestingly 68% of differentially expressed genes in GD overlap with that of RD, which makes it difficult for us to separate the lineages by examining several markers. In this paper, we design a prediction model to identify the differentiation into neural fates from any other lineage. From the profiles of 11,376 genes, 203 differentially expressed genes between neural and random differentiation were selected by random variance T-test with 95% confidence and 5% false discovery rate. Based on support vector machine algorithm, we could select 79 marker genes from the 203 informative genes to construct the optimal prediction model. Here we propose a prediction model for the prediction of neural fates from random differentiation which is constructed with a perfect accuracy.