This study conducted a survey to find out the opinions on oral health behavior and oral health education according to gender in health and non-health college students. According to the research results, there were many girls in the health-realted field and boys in the non-health-related college students. Among the oral health behaviors, brushing in school was common in both boys and girls in the health-related college students, and professional oral health education experiences were also found in the health-related college students. The need for oral health education among male students was 76.4% for healh-related college students, 48.3% for non-health-related college students, whereas female health-related college students showed 80.3%, and non-health -related college students were 60.4%. Participation in oral health education in order of male health-related students, male non-health-related students, female health-related students, and female non-health-related students were 81.9%, 68.1%, 84.8% and 73.3% respectively. The preferred method of oral health education was experiential education such as brushing for both male and female in the health-related college students, and lectures by dentists or dental hygienist were the highest reponse for non-health-related college students. The preferred location for oral health education was highest in schools. Through the results of this study, it was considered necessary to develop and disseminate appropriate oral health education programs according to college students' majors and gender, and to form correct oral health knowledge, attitudes and behaviors for oral health through oral health education.