• Title, Summary, Keyword: deactivated

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Reaction Characteristics of 4-Methylcatechol 2,3-Dioxygenase from Pseudomonas putida SU10

  • Ha, You-Mee;Jung, Young-Hee;Kwon, Dae-Young;Kim, Young-Soo;Kim, Chy-Kyung;Min, Kyung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.10 no.1
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    • pp.35-42
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    • 2000
  • Reaction characteristics of 4-methylcatechol 2,3-dioxygenase (4MC230) purified from Pseudomonas putida SU10 with a higher activity toward 4-methylcatechol than catechol or 3-cethylcatechol were studied by altering their physical and chemical properties. The enzyme exhibited a maximum activity at pH 7.5 and approximately 40% at pH 6.0 for 4-methylcatechol hydrolysis. The optimum temperature for the enzyme was around $35^{\circ}C$, since the enzyme was unstable at higher temperature. Acetone(10%) stabilized the 4MC230. The effects of solvent and other chemicals (inactivator or reactivator) for the reactivation of the 4MC230 were also investigated. Silver nitrate and hydrogen peroxid severely deactivated the enzyme and the deactivation by hydrogen peroxide severely deactivated the enzyme and the deactivation by hydrogen peroxide was mainly due to the oxidation of ferrous ion to ferric ion. Some solvents acted as an activator and protector for the enzyme from deactivation by hydrogen peroxide. Ascorbate, cysteine, or ferrous ion reactivated the deactivated enzyme by hydrogen peroxide. The addition of ferrous ion together with a reducing agent fully recovered the enzyme activity and increased its activity abut 2 times.

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Simultaneous detection method for pesticide residues in meat by gas chromatograph-mass selective detector (Gas chromatograph-mass selective detector를 이용한 식육 중 잔류농약의 동시분석)

  • Hong In-Suk;Choi Yoon-Hwa;Kweon Taek-Boo;Lee Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.28 no.3
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    • pp.285-294
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    • 2005
  • This study was conducted to investigate the extraction method for the determination of organochlorine, organophosphorus and synthetic pyrethroid pesticide residues in beef fat by gas chromatography-mass selective detector(GC-MSD). Twenty one pesticide residues in fat were determined using a simple and rapid procedure based on solid- phase extraction(SPE) clean-up cartridges with octadecyl $(C_{18})-bonded$ porous silica, florisil, $10\%$ deactivated florisil. a tandem $C_{18}$ and florisil and a tandem $C_{18}$ and $10\%$ deactivated florisil. Solvent-solvent extraction using acetonitrile was not satisfied to eliminate fat interference for pesticide residue analysis by GC-MSD, and the recoveries of the method in fat ranged from 16.2 to $57.3\%$ except DDT$(83.2\%)$. The recoveries of SPE methods using a tandem $C_{18}$ and Florisil was $59.6\~123.8\%$ except fenitrothion $(135.2\%)$. the SPE method was verified the satisfactory performance of pre-treatment for pesticide residues analysis in fat by GC-MSD. The efficiency of florisil deactivated with $10\%$ water has been not proved significantly on recoveries of pesticide residues in fat.

Deactivation of $V_2O_5/TiO_2$ catalyst used in Orimulsion Fuel Power Plant for the Reduction of Nox (배연 탈질용 $V_2O_5/TiO_2$ 촉매의 오리멀젼 연소에 의한 비활성화)

  • Lee, In-Young;Lee, Jung-Bin
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.1
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    • pp.54-60
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    • 2008
  • Deactivation of SCR catalyst applied in Orimusion fuel power plant was investigated to develope the technique for the regeneration of deactivated SCR catalyst and optimize the operation of SCR facility. The characterization study of the catalysts was carried out using XRD, ICP-AES, SEM and EDS. The NO$_X$ removal activity and SO$_2$ oxidation activity of the catalysts were measured. The NO$_X$ conversion of the deactivated catalyst was 5$\sim$10% lower than that of the fresh catalyst and the value of SO$_2$conversion to SO$_3$ over the deactivated catalyst was about 0.59% higher than that of the fresh catalyst. Vanadium(V), Magnesium(Mg) and Sulfur(S) were largely accumulated in the deactivated catalyst. The accumulation of Vanadium(V) and Sulfur(S) is due to the components of the Orimulsion fuel and the accumulation of Magnesium(Mg) is due to MgO that is injected in the boiler to prevent the oxidation of SO$_2$ to SO$_3$. The diffraction line of the TiO$_2$ of the deactivated catalyst was identified as the crystalline peaks of anatase as the fresh catalyst.

Recent Advances and Trends in Reactive Polyurethane Adhesives

  • Krebs, Michael
    • Journal of Adhesion and Interface
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    • v.7 no.4
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    • pp.53-59
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    • 2006
  • The paper highlights technical advances and introduces recent innovations such as smart curing laminating adhesives for flexible packaging with low migration rates of aromatic isocyanates and amines. Latent reactive one-part systems on the basis of surface deactivated solid isocyanates open up new dimensions for heat setting adhesives and waterborne PU dispersions. The new generation of Purmelt Micro Emission adhesives contains less than 0.1% of MDI monomer, thereby drastically reducing the emission of hazardous isocyanate vapors during processing and setting a significantly improved technical standard in occupational safety. Once again, polyurethane adhesives have demonstrated their unique ability to adapt to new process, product, safety and environmental requirements.

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A Study on the Prediction and Control of Welding Deformation of the BRACKET TILT in Automotive Parts (I) - Application of FEA- (자동차 부품 BRACKET TILT의 용접변형 예측 미 제어에 관한 연구 (II) -유한요소법의 적용-)

  • 장경복;강성수
    • Journal of Welding and Joining
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    • v.16 no.6
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    • pp.104-112
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    • 1998
  • In the previous study, the countermeasure for welding deformation of bracket tilt is through up through experimental inspection for total process including welding process. For completeness of systematic examination of parts having sensitivity on welding deformation, the comparison and feedback between the result through simulation of welding process and experimental data is needed. In other words, it is necessary to control welding deformation that construct the prediction system for welding deformation through comparison and tuning with experimental data. In the present study, the application of FEA on welding process of bracket tilt with susceptibility to deformation is made and deformation behavior through change of welding sequence is focused on. It is used to improve the exactness of deformation analysis that three dimensional analysis for moving heat source, activated and deactivated bead element, and volume heat flux etc.

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Influence of Flow Conditions of Intake Air on Gas Flow Patterns in Engine Cylinder (흡기 유동 조건의 변화가 실린더 내 가스 유동 패턴에 미치는 영향)

  • 이창식;전문수;김우경;최수천
    • Transactions of the Korean Society of Automotive Engineers
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    • v.7 no.8
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    • pp.17-23
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    • 1999
  • This paper presents the characteristics of the gas flow in the engine cylinder under various intake flow conditins. The particle tracking velovimetry(PTV) was used to anlayze the gas flow pattern and flow field in the cylinder. Effects of tumble intensifying valve(TIV), swirl intensifying valve(SIV) and one-valve deactivated condition on in-cylinder flow patterns were compared with the baseline engine udner 600rpm motoring condition. In addtion, tumbel ration was estimated rwith results of in -cylinder flow fields. Base on experimental results, the tumble ration of in-cylinder flow field has the maximum value at the bottom dead center for the different four inlet conditions. In TIV condition, the tumble ration is 1.35 times larger than that of baseline engine and 1 intake valve deactivated condition is effective to improve in-cylinder swirl motion.

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A Study on the Regeneration of SCR Catalyst Deactivated by Unburned Carbon Deposition (탄소침적으로 피독된 탈질 촉매의 재생에 관한 연구)

  • Moon, Seung-Hyun;Lee, Seung-Jae;Ryu, In-Soo
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.10
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    • pp.928-935
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    • 2010
  • A bag filter system was partially burnt down during a trial run of waste wood incineration boiler. This brought about unburned hydrocarbon which caused a rapid deactivation of low temperature SCR catalyst set up in two stage after the bag filter. The deactivated catalyst was investigated in order to trace the origin by several characterization methods such as XRD, EDX, BET, TGA, SEM. The deactivated catalyst was regenerated by different methods such as acid washing, water washing in ultrasonication, and calcination treatment under air condition. It is found the calcination treatment under air condition at $450^{\circ}C$ for 2 hours to be the best regeneration method. The catalytic activity was measured in the form of 2 cm ${\times}$ 2 cm ${\times}$ 10 cm (catalyst weight 10 g) honeycomb type. A deNOx efficiency of the regenerated catalyst showed 100% at $180^{\circ}C$ which is the same level of fresh one.

The Effect of SO2 in Flue Gas on the SCR Activity of V/TiO2 (배가스 중 SO2가 V/TiO2 SCR활성에 미치는 영향)

  • Hong, Sung-Chang
    • Applied Chemistry for Engineering
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    • v.17 no.5
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    • pp.490-497
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    • 2006
  • $V_{2}O_{5}$/$TiO_{2}$ catalyst can be deactivated by ammonium salts formed by $SO_{2}$ oxidation and unreacted ammonium in presence of $SO_{2}$ in flue gas. The deactivation of catalyst by $SO_{2}$ depends on the $SO_{2}$ oxidation to $SO_{3}$. The oxidation of $SO_{2}$ is weakly affected by oxygen concentration, and strongly by the amount of vanadium loaded onto titania supports. Because unreacted ammonia is one of elements to form the ammonium salts, it is important to control the mole ratio of $NH_{3}/NOx$ in SCR. Thus the experiments about $NH_{3}/NOx$ were carried out. The reason of low activity of catalyst deactivated by ammonium salts is the change of pore volume. And TPD (Temperature Programmed Decomposition) was performed to find the decomposition of ammonium bisulfate on deactivated catalyst.

Estimation for the Transfer Function of Transmission Line using the Temination and Input Impedances at Activated/Deactivated states (활성/비활성 상태에서의 종단과 입력 임피던스 변화를 이용한 전송선로의 전달함수 추정)

  • 이종헌;진용옥
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.17 no.1
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    • pp.90-97
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    • 1992
  • An estimation method for the amplitude and phase response of transmission line is discussed. and applied to narrow band ISDN subscriber line. The ABCD parameters of line are evaluated from four impedance values: the standard termination impedence at activated and deactivated stares, and the input impedances of line which can be estimated at each state. Estimating input impedence, the “chirp” signal is used as incident signal and noise effect can be reduced by ensemble averaging. These ABCD parameter estimations might be applicable to ether uniform or nonuniform line. Cleary the magnitude and phase response can be obtained from estimated ABCD parameters. The numerical simulation results for N ISDN subscriber line model are included, and the estimation error introduced by deviation in load impedence is also anlyzed.

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A Study on Remanufacturing of Deactivated Commercial Diesel Oxidation Catalyst by CVS-75 mode in Light Duty Diesel Engine (비활성화된 상용 디젤 산화 촉매의 소형 디젤 기관에서 CVS-75 모드를 이용한 재제조에 관한 연구)

  • Lee, Chang-Hee;Park, Hea-Kyung
    • Journal of Korean Powder Metallurgy Institute
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    • v.18 no.6
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    • pp.517-525
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    • 2011
  • In this study, the used DOCs, which could remove the air pollutants such as CO and HC in the exhaust gas from diesel vehicle, were remanufactured by various conditions. Their catalytic performances and characterization were also investigated. The remanufacturing process of the deactivated DOCs includes high temperature cleaning of incineration, ultrasonic cleaning for washing with acid/base solutions to remove deactivating materials deposited to the surface of the catalysts, and active component reimpregnation for reactivating catalytic activity of them. The catalytic performance tests of the remanufactured DOCs were carried out by the diesel engine dynamo systems and chassi dynamo systems in CVS-75 mode. All prepared catalysts were characterized by the optical microscopes, SEM, EDX, porosimeter and BET to investigate correlations between catalytic reactivity and surface characteristics of them. The remanufactured DOCs at various conditions showed the improved catalytic performances reaching to 90% of fresh DOC, which is attributed to remove the deactivating materials from the surface of the used DOC through the analysis of catalytic performance test and their characterization.