• Title, Summary, Keyword: cytotoxic activity

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Triterpenoids from Schisandra henryi with Cytotoxic Effect on Leukemia and Hela Cells In Vitro

  • Chen, Ye-Gao;Wu, Zheng-Cai;Lv, Yu-Ping;Gui, Shi-Hong;Wen, Jin;Liao, Xin-Rong;Yuan, Li-Ming;Halaweish, Fathi
    • Archives of Pharmacal Research
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    • v.26 no.11
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    • pp.912-916
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    • 2003
  • Four known lanostane triterpenoids, schiprolactone A (1), schisanlactone B (2), nigranoic acid (3) and schisandronic acid (4) Were isolated from the stems of Schisandra henryi for the first time. Their structures were characterized by IR, MS and NMR techniques. Compounds 1, 2 and 4 showed moderate cytotoxic activity against Leukemia cells in vitro. Cytotoxic activity of compounds 1-4 showed $IC_{50}$ of 0.0097, 0.01, 0.097 and 0.0099 $\mu$ mol/mL respectively toward Leukemia cells and $IC_{50}$ of 0.097, 0.1, 0.097 and 0.099 $\mu$mol/mL toward Hela cells respectively. It is the first report that these compounds possess cytotoxic activity on Leukemia and Hela cells.

Cytotoxic Activities of Red Algae Collected from Jeju Island Against Four Tumor Cell Lines

  • Kim, Kil-Nam;Lee, Ki-Wan;Song, Choon-Bok;Ahn, Chang-Bum;Jeon, You-Jin
    • Preventive Nutrition and Food Science
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    • v.11 no.3
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    • pp.177-183
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    • 2006
  • Methanolic and aqueous extracts of 26 red algae species collected from Jeju Island coast were prepared at a high $(70^{\circ}C)$ and a room temperature $(20^{\circ}C)$ and were examined for their cytotoxic activity against 4 tumor cell lines: U-937 (human monoblastoid leukemia cell line), HL-60 (human promyelocytic leukemia cell line), B-16 (murine melanoma cell line) and HeLa (woman cervical carcinoma cell line). $20^{\circ}C$ methanolic extract of Polysiphonia japonica showed cytotoxic activity of over 50% against U-937, HL-60 and B-16 cells. On the other hand, the $20^{\circ}C$ aqueous extract of Scinaia okamurae and $70^{\circ}C$ aqueous extract of Chondrus crispus showed cell growth inhibition activity of more than 50% against HL-60 and B-16 cells. The highest cytotoxic activity was observed in the $20^{\circ}C$ aqueous extract of Scinaia okamurae against B-16 cells (80.55%).

Cytotoxic and Antioxidant Effects of Taraxacum coreanum Nakai. and T. officinale WEB. Extracts (흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교)

  • Lee, Hyun-Hwa;Lee, Sook-Yeong
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.2
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    • pp.79-85
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    • 2008
  • Some studies of dandelion have been carried out on bioactivities, however, no comparative analysis on antioxidant and cytotoxic activities in the Korean dandelion (T. coreanum NAKAI.; KD) and dandelion (T. officinale WEB.) have been reported. In this study, the extracts of KD and dandelion analyzed relative phenolic contents and free radical scavenging, antioxidant enzyme and cytotoxic activities. The extract of Korean dandelion leaves (KDL) exhibited a higher phenolics content ($368{\pm}11.5\;mg/l00\;g$) and a strong free radical scavenging activity ($RC_{50}$value;87.89 ug/ml) than other parts and BHT (120.12 ug/ml), synthetic antioxidant. At ascorbate peroxidase (APX) activity, the dandelion root (DR) had a greater (38.8 U/mg protein) antioxidant enzyme compare to the dandelion leaves (12.2 U/mg protein). The catalase (CAT) and superoxide dismutase (SOD) followed higher enzyme activity in Korean dandelion root (KDR) than other parts. In a cytotoxic activity against human cancer cell, the extracts of KDR was found to be active against Calu-6, HCT-116, and SNU-601 cell lines, with $IC_{50}$ values of 522.34, 532.74 and 614.85 ug/ml, respectively. These results suggest that KD and dandelion would be an alternative antioxidant source, based on natural plant resources.

Synthesis and Cytotoxicity of New 3-Alkyl-1-(1-methyl-2-phenylethyl)ureas Related to Ceramide

  • Lim, Sejin;Ryu, Jae-Hark;Im, Chaeuk;Yim, Chul-Bu
    • Archives of Pharmacal Research
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    • v.26 no.4
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    • pp.270-274
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    • 2003
  • A series of new 3-alkyl-1-(1-methyl-2-phenylethyl)ureas related to ceramide was synthesized and evaluated for their in vitro cytotoxic activity against five human tumor cell lines. The urea analogue (2b) of 813 showed comparable or slightly more potent cytotoxic activity as compared to B13, indicating that urea does appear to serve as a bioisostere of amide.

Cytotoxic Quassinoids from Simaba cedron

  • Hitotsuyanagi, Yukio
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.52-55
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    • 1998
  • During a survey of new antitumor substances from higher plants, we have found that the crude extract of Simaba cedron Planchon (Simaroubaceae) showed cytotoxic activity (IC$\sub$50/ 0.7 $\mu\textrm{g}$/mL) against P388 leukemia cells. Activity-guided chromatographic purification using P388 cells led to the isolation of five novel quassinoids, cedronolactones A-E (1-5) and nine known quassinoids, simalikalactone D (6), chaparrinon (7), chaparrin (8), glaucarubolone (9), glaucarubol (10), samaderine Z (11), guanepolide (12), ailanquassin A (13), and polyandrol (14). In this seminar, the structural elucidation of 1-5 and the cytotoxic activity of the isolated compounds are discussed.

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Cytotoxic activity of (2S, 3R, 4E) 5-Aryl-4-pentene-1,3-diol-2-aminoueras

  • Im, C.U.;Kim, Y.H.;Jyung, M.L.;Lim, C.B.
    • Proceedings of the PSK Conference
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    • pp.248.2-248
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    • 2003
  • The 18 ureidoceramide derivatives had been investigated for their cytotoxic activity against HT-29 colon cancer, Caki-2 renal cancer, A549 lung cancer, PC-3 prostate cancer, HL -60 leukemia cell using MTT assay. Cytotoxic activity was strongly influenced by the substituted alkyl chain length and the optimal alkyl chain length for cytotoxicity was C9-C12. (omitted)

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Screening and Isolation of the Antitumor Agents from Medicinal Plants (I) (생약으로부터 항암성분의 검색 및 분리 (I))

  • Park, Shin-Young;Kim, Jin-Woong
    • Korean Journal of Pharmacognosy
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    • v.23 no.4
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    • pp.264-267
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    • 1992
  • The cytotoxic activity of medicinal plants was screened using A549 human lung cancer cell line. Plant materials were extracted with 80% methanol and fractionated to chloroform and water layers. Each methanol, chloroform, and water extract of thirty-two medicinal plants was tested for cytotoxic activity in A549 cell culture system and the cell viability was measured by SRB assay.

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In vitro cytotoxic activity of ginseng leaf/stem extracts obtained by subcritical water extraction

  • Lee, Kyoung Ah;Kim, Kee-Tae;Chang, Pahn-Shik;Paik, Hyun-Dong
    • Journal of Ginseng Research
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    • v.38 no.4
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    • pp.289-292
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    • 2014
  • Ginseng leaf/stem extract produced by subcritical water extraction at high temperature ($190^{\circ}C$) posses higher cytotoxic activity against human cancer cell lines than ethanol extract. Subcritical water extraction can be a great candidate for extraction of functional substance from ginseng leaves/stems.

Analgesic, cytotoxic and antioxidant activities of Trewia polycarpa bark

  • Rahman, Md Shafiur;Sadhu, Shamir Kumar;Hasan, Choudhury Mahmud
    • Oriental Pharmacy and Experimental Medicine
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    • v.6 no.2
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    • pp.121-125
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    • 2006
  • The crude ethanol extract of the stem bark of Trewia polycarpa (Family: Euphorbiaceae) was subjected to acetic acid induced writhing inhibition, Brine Shrimp lethality bioassay and 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay for screening of analgesic, cytotoxic and antioxidant activity respectively. The extract produced significant (P < 0.001) writhing inhibition in acetic acid induced writhing in mice at the dose of 125, 250 and 500 mg/kg body weight respectively, which were comparable to the standard drug diclofenac sodium. The extract showed significant lethality to Brine Shrimp and the $LC_{50}$ value was $8\;{\mu}g/ml$. The extract showed prominent free radical scavenging activity ($IC_{50}$ about ${\sim}10\;{\mu}g/ml$) compare to standard drug ascorbic acid ($IC_{50}about\;{\sim}15\;{\mu}g/ml$). The results tend to suggest that the crude ethanol extract of the bark might possess analgesic, cytotoxic and antioxidant activities or active constituent(s) responsible for the activities.

Sesquiterpene-Neolignans from the Stem Bark of Magnolia obovata and Their Cytotoxic Activity

  • Youn, Ui-Joung;Chen, Quan Cheng;Lee, Ik-Soo;Kim, Hong-Jin;Hung, Tran Manh;Na, Min-Kyun;Lee, Jong-Pill;Min, Byung-Sun;Bae, Ki-Hwan
    • Natural Product Sciences
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    • v.14 no.1
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    • pp.51-55
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    • 2008
  • Three sesquiterpene-lignans, eudeshonokiol B (1), eudesobovatol B (2), and clovanemagnolol (3), were isolated from the stem bark of Magnolia obovata, together with magnolol (4), honokiol (5), and obovatol (6) on the basis of spectroscopic and physicochemical analyses including 2D NMR and Mass. Compounds 1 - 3 were belongs to a unique class of natural products made up of a sesquiterpene and biphenyl-type neolignan via an ether bond. All the isolated compounds were tested in vitro for their cytotoxic activity against the HeLa, A549, and HCTll6 cancer cell lines. Compounds 1 - 6 showed the cytotoxic activity against tested cancer cell lines, with $IC_{50}$ values ranging from 7.1 to 14.4 ${\mu}g/mL$.