• Title, Summary, Keyword: cytotoxic activity

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Cytotoxic Activity of the Inula japonica Extracts Against Several Human Cancer Cell Lines In Vitro (선복화 (Inula japonica)추출물의 암세포주에 대한 In Vitro 세포독성)

  • Cha, Mi-Ran;Kim, Ju-Young;Hwang, Ji-Hwan;Park, Hae-Ryong
    • Korean Journal of Pharmacognosy
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    • v.37 no.3
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    • pp.130-135
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    • 2006
  • The present study describes the preliminary evaluation of the cytotoxic activity of the extracts from Inula japonica. I. japonica was extracted with methanol, ethanol, acetone, and water, and then cytotoxic activity of these extracts were evaluated. The cytotoxic activity of each extract was assessed by the MTT-dye reduction assay. Both ethanol and acetone extracts from I. japonica showed the cytotoxic activity against the HT-29 human colon cancer cells. Furthermore, the ethanol extract was fractionated with n-hexane, diethyl ether, ethyl acetate, and water according to degree of Polarity, The diethyl ether fraction showed the highest cytotoxic activity against HT-29 cells, but the other fractions showed low cytotokic activity. In addition, diethyl ether layer also showed the cytotoxic activity against various tumor cells, such as human colon carcinoma SW620, human cervix adenocarcinoma HeLa, and human breast adenocarcinoma MCF-7 cells as well as HT-29 cells. These studies support that extracts of I. japonica may be a potential candidate as possible chemotherapeutic agent against human cancer.

A Study on Relationship of Symptom Distress and Natural Killer Cell Cytotoxicity in Breast Cancer Patients (치료 중인 유방암 환자의 신체적 증상과 자연살해세포 활성도의 관계)

  • Chae, Young-Ran
    • Journal of Korean Biological Nursing Science
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    • v.4 no.2
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    • pp.69-77
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    • 2002
  • The purpose of this study was to identify the relationship of symptom distress and natural killer cell cytotoxicity in breast cancer patients who had been radiation therapy and/or chemotherapy after surgery. Symptom distress measured by modified Lee's(1994) physical symptom questionnaire. For measuring the natural killer cell cytotoxic activity. 8ml to 10ml blood was collected from the subjects. Mononuclear cell was isolated by centrifuge of the blood and cultured by putting $Cr^{51}$, and reacted with target cell, K562 cell. Amount of $Cr^{51}$ was measured, and %lysis was calculated. The results were as follows. 1) Symptom distress score was 42.18, which is moderate symptom distress. 2) Natural killer cell cytotoxic activities were 42.18%lysis(effector : target cell ratio=100 : 1) and 28.05%lysis(effector : target cell ratio=50 : 1). 3) Correlation coefficients of symptom distress and natural killer cell cytotoxic activity were $-.134{\sim}-.461$. Though significant correlation was not found between total score of symptom distress and natural killer cell cytotoxic activity, 3('pain' 'feel hot on radiation site' and 'difficulty in breathing') of 19 symptom distress items and natural killer cell cytotoxic activity showed significant negative correlation(p<.05). These findings suggest that 1) breast cancer patients who had been radiation therapy and/or chemotherapy after surgery have moderate symptom distress and decreased natural killer cell cytotoxic activity. 2) The symptom distress was not related to natural killer cell cytotoxic activity.

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Lupane Derivatives-I: Synthesis and Cytotoxic Activity (루판 유도체의 합성 및 세포독성-I)

  • You, Young-Jae;Kim, Yong;Ahn, Byung-Zun
    • YAKHAK HOEJI
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    • v.46 no.5
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    • pp.295-300
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    • 2002
  • To observe the structure-activity relationship of lupane derivatives both on cytotoxic and antiangiogenic activity, twelve lupane derivatives were prepared; their antiangiogenic and cytotoxic activities were evaluated. Among them, four compounds were more cytotoxic than betulinic acid. Carboxylic acid at C28 seemed to be essential for cytotoxic activity. But, a selective cytotoxicity toward SK-MEL-2 was not observed. As for antiangiogenic activity, none of the compounds except lupeol showed antiangiogenic activity at 30 $\mu\textrm{g}$/mL.

Steroids and Triterpenoid from the Fruit Bodies of Ganoderma lucidum and Their Cytotoxic Activity

  • Lee, Joon-Seok;Lee, Mi-Kyoung;Hung, Tran-Manh;Lee, Ik-Soo;Min, Byung-Sun;Bae, Ki-Hwan
    • Natural Product Sciences
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    • v.15 no.3
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    • pp.173-179
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    • 2009
  • To determine the cytotoxic activity of natural compounds, chromatographic separation of the hexanesoluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of four steroids and one triterpenoid. They were identified as ergosterol peroxide (1), stella sterol (2), ergosterol (3), 9(11)-dehydroergostrol peroxide (4), and ganodermanontriol (5) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their cytotoxic activity against HL-60, MCF-7, and LLC cancer cell lines. Ganodermanontriol (5) showed cytotoxic activity with IC$_{50}$ values of 24.8 and 22.9 $\mu$g/mL against HL-60 and MCF-7 cancer cell lines, respectively, whereas compounds 1 - 4 were inactive.

Synthesis and Cytotoxic Activity-II, Aminoacetyl Derivatives (루판 유도체의 합성 및 세포독성-II: 아미노아세칠 유도체)

  • You, Young-Jae;Kim, Yong;Nam, Nguyen-Hai;Ahm, Byung-Zun
    • YAKHAK HOEJI
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    • v.46 no.5
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    • pp.301-306
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    • 2002
  • To increase the water solubility, sixteen lupane derivatives bearing aminoacetyl moiety at C3 were synthesized. Their cytotoxic activities against three cancer cell lines, SK-MEL-2, A549, and B16, were tested. Among them, six derivatives showed significant cytotoxic activity.

Antifungal Effect of Brachyglottis repanda Ethanol Extract

  • Yook, Chan-Nam;Na, Young-Soon;Choi, Hwa-Jung;You, Il-Soo;Baek, Jong-Min;Baek, Seung-Hwa
    • Toxicological Research
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    • v.26 no.2
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    • pp.117-121
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    • 2010
  • The crude ethanol extract of B. repanda showed the cytotoxic activity against Polio virus (25% activity at $150{\mu}g$/disk) and the minor cytotoxic activity against BSC cells (African green monkey kidney). However, the crude ethanol extract of B. repanda was non-toxic to murine leukaemia cells CCL 46 P388D1 ($IC_{50}$, > 62,500 ng/ml). Cytotoxic and antifungal activities were strongly shown by Fr. 64-3 which was eluted with 90% $CH_3CN/H_2O$, 100% $CH_3CN$, and 50% $CH_3CN/H_2O$(SM 2 at $150{\mu}g$/disk). The fraction 64-3 also showed the most cytotoxic activity against murine leukaemia cells (128 mg, $IC_{50}$ 10,051 ng/ml at $75{\mu}g$/disk). These results suggest that this fraction has a potent antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185.

Antimicrobial and Cytotoxic Activities of Some Malaysian Flowering Plants

  • Jasril, Jasril;Mohamed, S.M.;Mackeen, M.M.;Lajis, N.H.;Rahman, A.A.;Ali, A.M.
    • Natural Product Sciences
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    • v.5 no.4
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    • pp.172-176
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    • 1999
  • A total of 43 extracts from 21 species of Malaysian flowering plants were screened for antimicrobial and cytotoxic activities. Antimicrobial activity was tested against fungi, including yeast and candida, as well as Gram-positive and Gram-negative bacteria, and cytotoxicity was assayed using the CEM-SS and HT-29 cell lines. The methanol extracts of the roots and stems of Plumeria acutifolia showed the highest antimicrobial activity, i.e. against Saccharomyces cerevisiae and Candida lipolytica, and mostly moderate activity against the other microbes such as Aspergillus ochraceous and Saccharomyces lipolytica. Phyllanthus emblica also showed moderate activity especially that of the methanol extracts. The methanol extract of the roots of Plumeria acutifolia showed the strongest cytotoxic activity $(CD_{50}=\;3\;{\mu}g/ml)$. Most of Zingiberaceae species gave negative results for antimicrobial activity and showed low cytotoxic activity.

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Cytotoxic activity of 1-phenyl-2-substituted thiourea derivatives

  • Im, C.U.;Park, Sang-Min;Jun, S.C.;Yim, C.B.
    • Proceedings of the PSK Conference
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    • pp.358.1-358
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    • 2002
  • The 20 thiourea derivatives had been investigated for their cytotoxic activity using MTT assay. Cytotoxic activity was strongly influenced by the substituted alkyl chain length. but not by configuration of Cl and C2. The optimal alkyl chain length for cytotoxicity was C12. The 9 thiourea derivatives showed stronger activity than reference compound. B13. some of them gave 2-3 times stronger activity than B13. (omitted)

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Cytotoxic Activities of Green and Brown Seaweeds Collected from Jeju Island against Four Tumor Cell Lines

  • Kim, Kil-Nam;Lee, Ki-Wan;Song, Choon-Bok;Jeon, You-Jin
    • Preventive Nutrition and Food Science
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    • v.11 no.1
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    • pp.17-24
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    • 2006
  • Methanolic and aqueous extracts from 37 seaweed species (10 green and 27 brown seaweeds) collected from Jeju Island coast were prepared at high ($70^{\circ}C$) and room ($20^{\circ}C$) temperatures and examined for cytotoxic activity against 4 tumor cell lines: U937 (human monoblastoid leukemia cell line), HL60 (human promyelocytic leukemia cell line), HeLa (woman cervical carcinoma cell line) and CT26 (mouse colon carcinoma line). Both MeOH extracts of Desmarestia tabacoides and Dictyota dichotoma possessed strong cytotoxic activities against all the tumor cell lines tested, but the aqueous extract exhibited no activity. On the other hand Ecklonia cava showed strong cytotoxic activities for the $20^{\circ}C$ aqueous extract against the three tumor cells except HeLa cell. Sagassum coreanum and Sagassum siliquastrum $20^{\circ}C$ aqueous extracts also exhibited strong cytotoxic activities against U937, HL60, HeLa cells. Even though green seaweeds showed less activity than brown seaweeds, $20^{\circ}C$ aqueous extracts of Codium contractum and Codium fragile exhibited strong cytotoxic activities against HL60 or CT26 cells, respectively.