• Title, Summary, Keyword: cucumber mosaic virus

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Symptoms of Cucumber Virus Diseases Occurred in Sangju and Gurye in 2006 and 2007 (2006년과 2007년 상주와 구례에서 발생한 오이 바이러스병의 병징 특성)

  • Cho, Jeom-Deog;Lee, Jung-Hwan;Ko, Sug-Ju;Choi, Hong-Soo;Lee, Su-Heon;Choi, Gug-Seoun;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.196-204
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    • 2011
  • Virus diseases occurring on cucumber was surveyed at main plantation areas of 'Sangju' and 'Gurye' in 2006 and 2007. Viral infection rate on cucumber was ranged from 14% to 90% in fields and the average infection rate was 46% at Sangju area. Cucumber cultivated at Gurye area had viral incidence ranged 9-100% and averaged 48%. The majorly infected viruses were Cucumber green mottle mosaic virus, Zucchini yellow mosaic virus, Papaya ring spot virus and Watermelon mosaic virus-2, and their infection rates were 23.5%, 13.0%, 9.0% and 2.0%, respectively in 2006 and 2007. Mixed infection rate of duplex, triplex and tetraplex was 31.5%, 7.5% and 2.5%, respectively. The rate of infection type was 47.7% by single infection, 31.5% by double infection and 88.7% by both infection types. Important viruses involved in mixed infection types were Zucchini yellow mosaic virus, Cucumber green mottle mosaic virus, Papaya ring spot virus and Broad been wilt virus2, orderly. Symptom in single infection was almost same on 'Dadagi' line and 'Chicheong' line of cucumber cultivars. Cucumber green mottle mosaic virus produced various symptoms of chlorotic spot, vein chlorosis and vein wrinkle and so on. Main symptoms of vein chlorosis, and severe mosaic and malformation were induced by Zucchini yellow mosaic virus. Mild symptoms were occurred relatively by Papaya ring spot virus and Watermelon mosaic virus2.

Incidence and Distribution of Virus Diseases on Cucumber in Jeonnam Province During 1999-2002

  • Ko, Sug-Ju;Lee, Yong-Hwan;Cha, Kwang-Hong;Lee, Su-Heon;Choi, Hong-Soo;Choi, Yong-Soo;Lim, Geun-Cheol;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.147-151
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    • 2006
  • Disease incidences of cucumber virus diseases in Jeonnam province were 52.5%, 16.1%, 35.2%, and 50.9% in 1999, 2000, 2001, and 2002, respectively. Rod- and flexuous rod-shaped virus particles were observed with the frequencies of 63.2% and 10.5%, respectively from the samples collected in 1999 under EM observation. Rod-shaped virus particles are considered as tobamovirus while flexuous rod shaped particles are considered as potyviruses. To further confirm their nature, total of 312 diseased virus samples were collected from 2000 to 2002, and tested by RT-PCR. Disease incidences of tobamoviruses including Cucumber green mottle mosaic virus and Kyuri green mottle mosaic virus were 48.7% and 3.8%, respectively while those of potyviruses including Zucchini yellow mosaic virus, Papaya ringspot virus, and Watermelon mosaic virus were 15.7%, 9.3%, and 5.1%, respectively. Interestingly, Cucumber mosaic virus was hardly detected. About 5.8% of tested samples were infected with more than one virus. Tobamovirus infection was consistently observed from September to December regardless of planting time, whereas infection of potyviruses was observed in many cucumber cultivating areas where it was planted in September and October.

Plant Disease Caused by Cucumber Mosaic Cucumovirus - Potential Role of Genes Associated with Symptom - (Cucumber Mosaic Cucumovirus에 의한 식물의 병 - 병징관련 유전자의 기능을 중심으로 -)

  • 최장경;김혜자
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.14-19
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    • 1999
  • Cucumber mosaic cucumovirus (CMV) is an isometric plant virus with functionally divided genomic RNAs and a broad host range. RNA 1 and RNA 2 each encode one protein, both of which are essential for replication. RNA 3 encodes the viral coat protein and an additional protein thought to be involved in potentiating the cell-to-cell movement of the virus. Functions of the RNAs have been confirmed using a pseudorecombinant virus constructed with infectious cDNA-derived transcripts of the RNAs. Generally, CMV produces different symptoms in various host plants depending on the virus strains. In this mini-review, we describe the potential role of the genes associated with symptom expression of CMV RNAs.

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Occurrence of Two Tobamovirus Diseases in Cucurbits and Control Measures in Korea

  • Park, Gug-Seoun
    • The Plant Pathology Journal
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    • v.17 no.5
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    • pp.243-248
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    • 2001
  • Two Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV) and Zucchini green mottle mosaic virus (ZGMMV), occurred in Korea in 463 ha in 1998, 33.9 ha in 1999, and 44.2 ha in 2000. CGMMV was detected in watermelon, cucumber, oriental melon, and melon, whereas ZGMMV was mainly detected in zucchini squash. Thirty-six CGMMV isolates wee classified into three types by analysis of single strand cDNA conformational polymorphism (SSCP) of the coat protein gene. In a comparison of serological relationships among CGMMV, ZGMMV, and Kyuri green mottle mosaic virus (KGMMV), the three tobamoviruses specifically reacted with each homologous antibody in the double-antibody sandwich enzyme-linked immunosorbent assay and rapid imunofilter paper assay (RIPA), although ZGMMV and KGMMV were slightly biologcially similar. In a survey of the three tobamoviruses in cucurbitgrowing field in Korea by RIPA, CGMMV and ZGMMV were detected but KGMMV was not found in commercially growing cucurbit crops so far. Seed contamination ratio of CGMMV in bottle gourd seeds tested was 84%, while seed trasmission ratio from the virus-contaminated seeds was 2.0%. Soil transmission ratio was 0-3.5% in fields naturally infested with CGMMV or ZGMMV. Control measures of the virus diseases are roguing and sanitation. These suggest that it is important to rogue the first infected crops, which include the seed and soil, especially early in the season. This may be practicable to control the diseases because CGMMV and ZGMMV have a narrow host range restricted to cucurbitaceous crops.

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Occurrence of Virus Diseases on Cucumber in Gyeongbuk Province (경북지역 오이에 발생하는 주요 바이러스 종류 및 발생실태)

  • Lee, Joong-Hwan;Kim, Dong-Geun;Ryu, Young-Hyun;Lee, Key-Woon
    • Research in Plant Disease
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    • v.14 no.2
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    • pp.138-141
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    • 2008
  • Cucumber is high valued cash crop, for it is grown during the winter season in plastic house. Recently, virus disease spread widely in cucumber growing area and cause severe income loss. Therefore, occurrence of virus disease on cucumber were surveyed from 2004 to 2006 in Sangju and Gunwi area, Gyeongbuk province. The rate of plastic house which has infected plants was $55.0{\sim}88.6%$. Infection rate was the highest at Sangju in 2006 than others and ranged from 15 to 90.0% per plastic house. The 217 samples showing virus symptom were analyzed by RT-PCR using appropriate detection primer. Zucchini yellow mosaic virus(ZYMV) has the highest infection rate(detected over 85%) and followed by Cucumber green mottle mosaic virus(CGMMV). But Watermelon mosaic virus-2(WMV-2) was not detected in our survey. Therefore, we conclude that ZYMV is major pathogene of virus disease on cucumber. ZYMV induced chlorosis and severe mosaic on the leaves and distortion on the surface of fruits.

Characterization and Sequence Analysis of a Lily Isolate of Cucumber mosaic virus from Lithium tsingtauense

  • Ryu, Ki-Hyun;Park, Hye-Won;Park, Jang-Kyung
    • The Plant Pathology Journal
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    • v.18 no.2
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    • pp.85-92
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    • 2002
  • A new isolate of Cucumber mosaic virus (CMV), identified as Li-CMV was isolated from a diseased Korean native lily (Lithium tsingtauense Gilg). Biological and serological properties of Li-CMV were characterized, and reverse transcription-polymerase chain reaction (RT-PCR) analysis, restriction enzyme profiling of RT-PCR products, and nucleotide sequence analysis of RNA3 of the virus were performed in this study. Remarkable differences in symptoms between Li-CMV and ordinary CMV strains were found in tobacco plants and Datura stramonium. Li-CMV-infected tobacco plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic ringspots on uninoculated upper leaves, and the symptom expression was delayed or faint whereas, ordinary CMV strains induced green mosaic symptoms on the plant. Systemic infections were observed on Nicotiana benthamiana with severe mosaic symptom. Restriction mapping analysis of RT-PCR products using MspI showed that Li-CMV belonged to CMV subgroup I. A full-length CDNA copy of RNA3 for the virus was amplified by RT-PCR, cloned, and its complete nucleotide sequence was determined. The RNA3 of Li-CMV was 2, 232 nucleotides long, and consisted of two open reading frames of 843 and 657 bases encoding 3a protein (movement protein) and coat protein, respectively. Results of this study indicate that Li-CMV is a novel strain and belongs to subgroup I of CMV in the genus Cucumovirus.

Color Breaking Syndrome of Matthiola incana Caused by Double Infection of Cucumber Mosaic Virus and Turnip Mosaic Virus (오이 모자이크 바이러스와 수눔 모자이크 바이러스의 복합감염에 의한 스톡의 꽃잎얼룩무늬병)

  • 윤주연;최홍수;류화영;함영일;최장경
    • Korean Journal Plant Pathology
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    • v.14 no.3
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    • pp.220-222
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    • 1998
  • In 1995, we collected stock (Matthiola incana) plants causing mosaic symptoms on leaves and color breakings on flowers in Daekwallyong, Korea. Two viruses were isolated from the infected plants, and identified as cucumber mosaic virus (CMV) and turnip mosaic virus (TuMV) by experiments of host range, serology and electron microscopy. Each of the virus did not produce the same symptoms on the stock seedlings as naturally infected plants caused. When the viruses were coinoculated to the stock seedlings, however, severe mosaic symptoms were observed on leaves, and then the color breakings were expressed on flowers.

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Ultrastructural Aspects of the Mixed Infections of Watermelon Mosaic Potyvirus and Cucumber Green Mottle Mosaic Tobamovirus Isolated from Watermelon

  • Kim, Jeong-Soo;Cho, Jeom-Deog;Park, Hong-Soo;Kim, Kyung-Soo
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.211-215
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    • 2000
  • Symptoms on 4 varieties of watermelons inoculated with watermelon mosaic potyvirus II isolated from watermelon (WMV-W) were severe mosaic and leaf malformation while those inoculated with cucumber green mottle mosaic tobamovirus from watermelon (CGMMV-W) were mild mosaic and chlorotic spots. Inoculation of the mixture of WMV-W and CGMMV-W produced extremely severe mosaic along with necrotic spots and general necrosis. Doubly infected plants were also stunted. Cells infected with WMV-W or CGMMV-W alone exhibited the intrinsically ultra-structural properties of each virus infection. WMV-W induced potyvirus-characteristic cylindrical inclusions in the cytosol. Virus particles were orderly aligned along the tonoplasts. CGMMV-W induced tobamovirus-characteristic stacked crystalline arrays of virus particles in the cytosol. Cells infected doubly with WMV-W and CGMMV-W contained striking cytopathic effects that were not present in single infection of each virus. The unique ring structure, nonagon, was that a single potyvirus particle was surrounded by 9 CGMMV-W tobamovirus particles.

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Multiplex Reverse Transcription Polymerase Chain Reaction Assay for Simultaneous Detection of Five Cucurbit-infecting Viruses.

  • Lee, Su-Heon;Kim, Sang-Mok;Kim, Woo-Chang;Lee, Key-Woon
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • pp.150.1-150
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    • 2003
  • A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses: cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.

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Occurence of Viruses in Lilies (Lilium spp.) in Highland Areas and Their Detection by One-step RT-PCR (고랭지 나리의 바이러스 발생과 RT-PCR에 의한 검정)

  • 김수정;함영일;신관용;류승열;유동림;정효원;최장경
    • Research in Plant Disease
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    • v.7 no.2
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    • pp.80-85
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    • 2001
  • This study was carried out to examine tne incidences of virus diseases in lily plants cultivated in highland areas, and to develop an effective detection method. Viral symptoms on lilies in the highland areas were differentiated into mosaic, crinkle, mottle, stripe and line pattern. The distribution of symptoms on infected plants was 43.8% of mosaic, 29.2% of crinkle, and 10.9% of mottle symptoms. Six viruses such as Lily symptomless vires(LSV), Cucumber mosaic virus (CMV), Lily mottle virus (LMoV), Lily virus X (LVX, Potexvirus), Tabacco mosaic virus (TMV,Tobamovirus), and Tabacco rattle virus (TRV,Tobravirus) were detected from the infected lilies. Infection rate of Lilium oriental (cvs. Casablanca and Marcopolo) was 2~4 times higher than that of L. asiatic (cvs. Solemio and Prato). Virus detection on lilies by one-step RT-PCR (by using reverse transcription and polymerase chain reaction simultaneously) was more rapid rapid and reliable than by the conventional RT-PCR method.

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