• Title, Summary, Keyword: cryIVD 유전자

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Characterization of Synechocystic PCC6803 transformed with cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 and its mosquitochidal effect on Anopheles sinensis (Bacillus thuringiensis subsp. morrisoni PG-14 cryIVD 유전자로 형질전환된 Synechocytis PCC6803의 특성과 학질모기에 대한 살충효과)

  • 이대원;박현우;김호산;진병래;유효석;김근영;강석권
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.66-73
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    • 1996
  • For the effective control of mosquito larvae, Anopheles sinensis, the expression vector pCYASK5-1 containing cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 was constructed and transformed into the cyanobacterium Synechocystis PCC6803. The transformants were selected on BG-11 medium containing kanamycin. The expression of cryIVD gene in transformant was confirmed by SDS-PAGE and Western blot analysis. The mortality of A. sinensis larvae was scored for 3 days. Furthermore, growth and distribution rate of transformant were examined. The results showed that Synechocystis PCC6803 transformed with cryIVD gene of B. thuringiensis subsp. morrisoni PG-14 was highly toxic to A. sinensis larvae, demonstrating that it will be a potential agent for mosquito control.

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Synergistic Effect in Mosquitocidal Activity of Crystal Proteins from Bacillus thuringiensis NT0423 Transformed with cryIVD Gene (cryIVD 유전자로 형질전환된 Bacillus thuringiensis NT0423 균주 내독소 단백질의 모기 유충에 대한 독성의 상승효과)

  • 박현우;유용만;오현우;진병래;김인기;강석권
    • Korean journal of applied entomology
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    • v.35 no.1
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    • pp.85-90
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    • 1996
  • Bacillus thuringiensis NT0423 produces quite a typical bipyramidal crystals of a common major band of ca. 130 kDa, and has dual specificity against Lepidoptera and Diptera. To enforce the Diptera-toxicity of B. thuringiensis NT0423, cryND gene was transformed 30 B. thuringiensis NT0423. The transfonnant B. thuringiensis PT1227 was obtained from introduction of pCGl0 into B. thuringiensis NT0423 by electroporation. The result showed that cryND and resident crystal protein genes in transformant were stably expressed with its own shape. Furthermore, the toxicity of B. thuringiensis PT1227 against Diptera was highly enforced, suggesting that the enforced toxicity of B. thuringiensis PT1227 was due to synergistic effect of both introduced and resident crystal proteins in transformant.

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Expression and Characterization of Three Types of $\delta$-Endotoxin Genes in Transformant, Bacillus thruingiensis PT0529 (형질전환체, Bacillus thuringiensis PT0529내에서 세가지 내독소 단백질 유전자들의 발현 특성)

  • 박현우;제연호
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.176-180
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    • 1995
  • To characterize expression and formation of three type crystal proteins in transformant, Bacillus thruingiensis PT0529 was analysed by transmission electron microscope and SDS-PAGE according to growth. The results showed that the introduced crystal protein genes, rcyIVD and cytA, were well expressed at earlier stage than resident crystal proteins were also expressed with their own morphology. However, resident crystal protein of B. thuringiensis PT0529 was smaller than that of wild type B. thuringiensis NT0423, suggesting that resident crystal protein production was interfered with introduced two type crystal protein genes.

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