• Title, Summary, Keyword: chlortetracycline

Search Result 98, Processing Time 0.052 seconds

Monitoring of Veterinary Antibiotics in Agricultural Soils using Liquid Chromatography Coupled with Tandem Mass Spectrometry (LC-MS/MS를 이용한 농경지 토양 중 항생제 모니터링)

  • Lee, Young-Jun;Choi, Jeong-Heui;Chung, Hyung Suk;Lee, Han Sol;Park, Byung-Jun;Kim, Jang-Eok;Shim, Jae-Han
    • Korean Journal of Environmental Agriculture
    • /
    • v.35 no.3
    • /
    • pp.166-174
    • /
    • 2016
  • BACKGROUND: The current study developed a monitoring method of 6 veterinary antibiotics (amoxicillin, ampicillin, enrofloxacin, tetracycline, chlortetracycline, oxytetracycline) in agricultural soils using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive electrospray ionization mode.METHODS AND RESULTS: Sample preparation was carried out using acidic acetonitrile and citrate salts followed by purification with dispersive solid phase extraction (d-SPE). Separation on Eclipse Plus C18 column was conducted in gradient of the mobile phase, 0.1% formic acid and 5 mM ammonium formate in methanol (A) and 0.1% formic acid and 5 mM ammonium formate in distilled water (B). The linearity of the matrix-matched calibrations expressed as the coefficient of determination was good with R2≥0.9900. The limit of quantifications (LOQs) ranged from 0.5 to 10 μg/kg for all analytes. Analysis of 51 agricultural soil samples taken in the Republic of Korea revealed concentrations less than 1.9 μg/kg for enrofloxacin, 75.5 μg/kg for chlortetracycline.CONCLUSION: The method was successfully applied to monitor 6 veterinary antibiotics from 51 field incurred agricultural soil samples in 17 provincial areas throughout the Republic of Korea. The developed method was simple, easy, and versatile and can be used for monitoring various veterinary antibiotics in soil.

Genetic and Physiological Characterization of Oxytetracycline-Resistant Bacteria from Giant Prawn Farms

  • Heepngoen, Pimpak;Sajjaphan, Kannika;Ferguson, John A.;Sadowsky, Michael J.
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.2
    • /
    • pp.199-206
    • /
    • 2008
  • Four hundred and thirteen oxytetracycline-resistant bacteria were recovered from six freshwater giant prawn farms with a history of oxytetracycline use. Most oxytetracycline-resistant isolates were Gram-negative bacteria. Six groups of oxytetracycline-resistant bacteria were classified using cluster analysis based on a comparison of levels of oxytetracycline resistance. Complex fingerprint patterns were obtained for 71 isolates studied. In general, the band patterns of isolates from different ponds were very similar, and the data indicated that the isolates were closely related. The exploration for cross-resistance found that most of the 71 oxytetracycline-resistant isolates were also resistant to tetracycline and chlortetracycline, but had a relatively low resistance to doxycycline. Many isolates showed higher chlortetracycline resistance than oxytetracycline resistance. Additionally, the oxytetracycline-resistant isolates were examined for the presence of tetracycline resistance (tet) genes. Fifty percent of the isolates carried one of the 14 known tet genes examined. The most common determinants were TetA and TetD. However, TetB, TetC, TetE, TetK, TetL, and TetM were also found with various frequencies.

Characteristics of Adsorption and Biodegradation of Tetracycline Antibiotics by Granular Activated Carbon and Biofiltration Process (Tetracycline계 항생물질들의 활성탄 흡착 및 생물여과 공정에 의한 생분해 특성)

  • Son, Hee-Jong;Yeom, Hoon-Sik;Ryu, Dong-Choon;Jang, Seung-Ho;Son, Hyung-Sik
    • Journal of Environmental Science International
    • /
    • v.23 no.3
    • /
    • pp.379-386
    • /
    • 2014
  • Adsorption and biodegradation performance of tetracycline antibiotic compounds such as ttetracycline (TC), oxytetracycline (OTC), minocycline (MNC), chlortetracycline (CTC), doxycycline (DXC), meclocycline (MCC), demeclocycline (DMC) on granular activated carbon (GAC) and anthracite-biofilter were evaluated in this study. Removal efficiency of seven tetracycline antibiotic compounds showed 54%~97% by GAC adsorption process (EBCT: 5~30 min). The orders of removal efficiency by GAC adsorption were tetracycline, demeclocycline, oxytetracycline, chlortetracycline, doxytetracycline, meclocycline and minocycline. Removal efficiencies of seven tetracycline antibiotic compounds showed 1%~61% by anthracite biofiltration process (EBCT: 5~30 min). The highest biodegradable tetracycline antibiotic compound was minocycline, and the worst biodegradable tetracycline antibiotic compounds were oxytetracycline and demeclocycline.

Effect of dietary chlortetracycline on productivity and respiratory disease in pigs (Chlortetracycline(CTC)의 사료 첨가가 돼지의 생산성과 호흡기 병변에 미치는 영향)

  • Kwon, Young-bang;Lee, Won-hyung;Lee, Wan-kyu;Kang, Jong-koo;Jung, Young-chul;Kim, Sung-hoon;Han, Byung-woo
    • Korean Journal of Veterinary Research
    • /
    • v.40 no.2
    • /
    • pp.345-360
    • /
    • 2000
  • The effects on CTC (T1) and CTC, Sulfathiazole and Penicillin Combination (T2) medication in feed through one life cycle of pigs, namely, weaning, mating, farrowing, lactation, growing, finishing and slaughter, were tested under local condition. In sow phase, productivity and the number of microflora in urine before and after medication of CTC were studied and average daily gain and feed conversion rate were checked during growing and finishing period. All pigs reached at 155 days old were slaughtered for pathological examination. 1. Litter size, farrowing rate and survival rate at birth were improved by CTC medication from weaning to 21th day after mating and mortality of piglet at weaning, 25 days after farrowing, was reduced in the CTC medication group, but no siginificant. 2. The number of microflora in the sow urine was changed with the medication at 200ppm of CTC in feed. In particular, the number of E coli, Samonella and Staphylococci were reduced by CTC medication. 3. The average daily gain and feed conversion rate of grower and finisher pigs was improved significantly in both treated groups, most in the high level CTC (T1) medicated group and was lowest in the control group. 4. The number of infected lungs was reduced not significant by both treatments (as % pneumonic lesions Co 66.7%, T1 47.1%, T2 31.4%) and the severity of lung lesions was significantly reduced by both high level of CTC and CTC combination medication in feed. 5. Although there were no statistical differences in atrophic rhinitis based on turbinate scores among the 3 groups, the number of mild and moderate (Grade 2 and 3) infections was higher in the control group (9/36) than in the treated groups (T1 2/34 & T2 4/35).

  • PDF

Effects of Dietary Supplementation of Rare Earth and Chlortetracycline on the Performance and Intestinal Microflora in Broiler Chicks (희토와 클로르테트라시이클인이 육계의 생산성 및 장내 미생물에 미치는 영향)

  • Song, T.H.;Park, H.S.
    • Korean Journal of Poultry Science
    • /
    • v.34 no.1
    • /
    • pp.23-29
    • /
    • 2007
  • Rare earth(RE) is a common name indicating 17 of specific elements including 15 of lanthanides, scandium (Sc), and yttrium(Y). This study was conducted to compare the effect of RE to growth stimulating antibiotic products in broiler diet. One of the products commonly used in broiler feeding, Chlorotetracycline(CTC) was used at a level of 200ppm for the antibiotic treatment and replaced by 100ppm RE for the RE treatment. Dietary addition of RE and CTC showed similar effects in improving broiler growth and feed conversion each other, and those were significantly better when compared to those of the control group(P<0.05). Abdominal fats of chicks fed RE were significantly lower than others(P<0.05). Intestinal E. coli and total microbial numbers were reduced by RE addition(P<0.05), but the number of Lactobacillus was not affected. The number of Salmonella was also decreased but it was not significant.

Antibiotic Uptake Reducing Effect of Zeolite and Shell Meal Fertilizer Amendment for Lettuce (Lactuca sativa L.) Cultivation Fertilized with Chicken Manure Compost

  • Seo, Youngho;Lim, Soojeong;Choi, Seungchul;Heo, Sujeong;Yoon, Byeongsung;Park, Younghak;Hong, Daeki
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.51 no.1
    • /
    • pp.1-7
    • /
    • 2018
  • The veterinary antibiotics treated to livestock have a potential risk to reach to soil and water environment, and eventually be taken up by plants. The objective of the study was to investigate the effect of zeolite and shell meal fertilizer amendment on antibiotic uptake by plant when veterinary antibiotics in chicken manure compost were applied to agricultural land. Model antibiotics used in the study were chlortetracycline (CTC), tylosin (TYL), and sulfamethazine (SMT). Chlortetracycline level in lettuce was decreased to less than $0.08ug\;kg^{-1}$ by application of zeolite as compared with about $0.26ug\;kg^{-1}$ for control without amendment on 33 days after transplanting. Tylosin was not detected for all the treatment. Sulfamethazine levels in lettuce ranged from 11 to $19{\mu}g\;kg^{-1}$ on a fresh weight basis and gradually decreased with time. Zeolite application decreased the SMT levels in lettuce by greater extent than shell meal fertilizer amendments. Results from the 61-d greenhouse experiment imply that application of zeolite at a rate of $1.5Mg\;ha^{-1}$ or shell meal fertilizer at a rate of $2.0Mg\;ha^{-1}$ can reduce CTC and SMT concentration in lettuce cultivated in soil fertilized with antibiotic-contaminated chicken manure compost.

Studies on HOSS test and CTC test for Viability and Capacitation of Frozen-thawed Canine Sperm (개 정맥의 동결 및 융해후 정자의 생존성 및 수정능획득 판정을 위한 HOS test 및 CTC test)

  • 김용준;지동범;오홍근
    • Journal of Veterinary Clinics
    • /
    • v.17 no.2
    • /
    • pp.431-437
    • /
    • 2000
  • Evaluation of viability and capacitation of canine sperm is of great importance to deter- mine good condition for freezing canine semen and consequently to improve conception rate by arti-ficial insemination. Semen were collected from nine male dots which had been proved to be fertile in the post and the semen were treaded for freezing procedure. Semen were thawed at 37$^{\circ}C$ for 30 seconds. In this study, hypoosmotic swelling(HOS) test and chlortetracycline(CTC) test were per- formed to evaluate post-thaw viability and capacitated status of sperm, respectively. In HOS test far canine sperm, the highest percentage of curled sperm was shown at 60 mOsm. In HOS test for canine semen, there were considerably significant correlation between HOS values and sperm motil- ity(r=0.9064, p<0.01) and converse correlation between HOS values and sperm abnormality(r=- 0.6905, p<0.05). The sperm viability and HOS-values for chilled extended semen were significantly decreased from 0 to 72 hours during storage at 5$^{\circ}C$ (p<0.05). Of the media added to canine semen after thawing, the most capacitated sperm were shown in CCM(p<0.01), and then This Fructose Cit- rate(TFC) medium with calcium from 3 hours after incubation with media. It was concluded that HOS test is of great value to determine the viability and motility of canine sperm, whereas CTC test is usable to determine the capacitated status. Consequently, both tests were thought to be useful as the additional tests to standard semen analysis.

  • PDF

Studies on the Content and Heat Decomposition of Residual Tetracycline in Meats on the Market (시판 육류중의 Tetracycline계 항생물질 잔류량과 가열분해에 관한 연구)

  • 배기철;이영근
    • Journal of Food Hygiene and Safety
    • /
    • v.6 no.2
    • /
    • pp.83-87
    • /
    • 1991
  • Beef, pork and chicken on the market were analyzed for determination of tetracyclines residue and decomposition of tetracyclines by heating were studied. The content of oxytetracycline was trace in chicken A, 0.09 mg/kg in C and trace in beef C, pork A and B, but in the other samples was not detected oxytetracycline. Chlortetracycline residue was 0.14, 0.02 and 0.01 mg/kg in chicken A, C and beef B respectively. In HPLC analysis, two peaks of 8.1 and 9.0 min of retention time were found from beef and pork and expected to be component of meat, and because of same retention time, the one of 9.0 min interrupted determination of tetracycline. But those were not found in chicken, therefore the residue was 0.01 mg/kg in sample A. The residue were degradated rapidly by heating of roast, but slightly by that of boiling.

  • PDF

In Vitro Alternative Phototoxicity Test for Various Cosmetic Natural Extracts and Chemicals (수종의 화장품용 천연물과 화합물의 In Vitro 광독성 대체 시험)

  • Cho, Wan-Goo;Park, Jee-Eun;Park, Mun-Euek;Lee, Sang-Min
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.35 no.3
    • /
    • pp.193-202
    • /
    • 2009
  • This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including $Medimin^{(R)}$ A, $Medimin^{(R)}$ D, $LG^{(R)}$ 106W, $Phytoclear^{(R)}$ EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, $Parsol^{(R)}$ MCX and $Parsol^{(R)}$ 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.