• Title, Summary, Keyword: cellular morphology

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The effects of ascorbic acid on the morphology and cell proliferation of three-dimensional stem cell spheroids (아스코르브산의 3차원 줄기세포 배양체에 대한 증식 및 분화 효과 분석)

  • Lee, Hyunjin;Yeo, Seong-Il;Park, Jun-Beom
    • The journal of the Korean dental association
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    • v.55 no.9
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    • pp.592-603
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    • 2017
  • Purpose: The effects of various concentrations of ascorbic acid on stem cell spheroids derived from intraoral areas are not known yet. Thus, the purpose of this study is to evaluate the effects of different concentrations of ascorbic acid on the morphology and cellular viability of stem cell spheroids derived from the gingival tissues. Materials and Methods: Stem cells were plated onto silicon elastomer-based concave microwells and grown in the presence of ascorbic acid at concentrations ranging from 0.003% to 0.3%. The morphology of the cells was viewed under an inverted microscope at day 1, 2, 3 and 5. Qualitative live/dead assay and quantitative cellular viability using Cell Counting Kit-8 were performed on day 2 and day 5. Results: Gingiva-derived stem cells formed spheroids irrespective of ascorbic acid concentration in silicon elastomer-based concave microwells. Increase in the diameter of spheroid were seen with higher concentrations of ascorbic acid. Higher cellular viability was seen in higher concentrations of ascorbic acid. Conclusion: Within the experimental setting, the application of ascorbic acid on stem-cell spheroids produced an increase in the size and higher viability with higher dosage. It can be suggested ascorbic acid be applied with stem cell spheroids for tissue engineering purposes.

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Effects of Hyperbaric Pressure on Cellular Morphology, Proliferation and Protein Expression of Jurkat Cell

  • Oh, Eun-Ha;Oh, Sang-Nam;Im, Ho-Sub;Lee, Joo-Hyun;Kim, Jin-Young;Moon, Joo-Hee;Hong, Eun-Young;Kim, Yang-Hee;Yang, Min-Ho;Lim, Yong-Chul;Park, Sun-Young;Lee, Eun-Il;Sul, Dong-Geun
    • Molecular & Cellular Toxicology
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    • v.1 no.2
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    • pp.116-123
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    • 2005
  • The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% $CO_{2}$, temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.

Effect of Titanium Coating on Cell Adhesion and Extracellular Matrix Formation in Human Osteoblast-like MG-63 Cells

  • Lee, Jae-Bum;Seo, Sang-Hui;Kim, Yu-Ri;Shin, Sang-Wan;Kim, Meyoung-Kon;Ryu, Jae-Jun
    • Molecular & Cellular Toxicology
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    • v.4 no.3
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    • pp.192-198
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    • 2008
  • A variety of titanium (Ti) and its alloys are used in the clinical procedures of bone regeneration for periodontal and dental implant therapies. This study was performed to determine the effect of different surface dental implant materials on biologic responses of a MG-63 human osteoblast-like cell line. MG-63 cells were cultured on Ti coated with hydroxyapatite (HA), calcium metaphosphate (CMP), anodized (A), which compared with non-coated Ti (control). The appearances of surface of dental implant materials and the morphology of these cells were assessed by scanning electron microscopy (SEM). The gene expression profiles of MG-63 cells cultured on Ti were examined by human cDNA microarray (1,152 elements). The expression of several genes was up- and down-regulated by different surfaces of dental implant materials. Interesting, the genes correlated with cellular adhesion and extra cellular matrix (ECM) formation were enhanced, in accordance surface morphology of the dental implant materials used.

A Research on Reflectivity of Microcellular Polypropylene (MCPs의 반사 특성에 관한 연구)

  • Seo Jung-Hwan;Cha Sung-W.;Kim Hak-Bin
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • pp.1367-1370
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    • 2005
  • Microcellular foam processing of polymers requires a nucleated cell density greater than $10^9\;cells/cm^3$ so that the fully grown cells are smaller than 10 mm. A microcellular foam can be developed by first saturating a polymer sample with a volatile blowing agent, followed by rapidly decreasing its solubility in the polymer. In general, the cellular structure of crystalline polymer foams is difficult to control, compared to that of amorphous polymer foams. Since the gas does not dissolved in the crystallites, the polymer/gas solution formed during the microcellular processing is nonuniform. Moreover, the bubble nucleation is nonhomogeneous because of the heterogeneous nature of the crystalline polymer. In this paper, the effects of the crystallinity and morphology of crystalline polymers on the microcellular foam processing and on reflectivity of products are investigated. First, polymer specimens with various morphology and amount of solved blowing agent were prepared by varying the saturation pressure, saturation time and foaming condition. Then, cell morphologies according to several conditions were studied. The specimens with differing gas amount of solved and morphologies were foamed and their cellular structures were compared. The experimental results of reflectivity are compared to raw specimen and another specimen of different experimental conditions. After the experiments, recognize whether how reflectivity changes according to solved gas amount. And the effect of cell density and cell size on reflectivity is studied

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The shrinkage characteristics of MCPs (Micro Cellular Plastics) (초미세 발포 플라스틱의 수축률 변화)

  • Seo, Jung-Hwan;Cha, Sung-Woon;Hyun, Chang-Hoon
    • Proceedings of the KSME Conference
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    • pp.1160-1164
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    • 2004
  • Generally in the case of parts used for precision products, tolerance of parts is very small. So inaccuate size of molding parts generates serious problems. Therefore, it's necessary to secure data about shrinkage on each condition or study about manufacturing process which reduces shrinkage. To apply MCPs to manufature of plastic product, this paper verifies how the amount of gas and Talc can affect to cell-morphology, and examines the relation between shrinkage and cell-morphology by using ASTM specimen formed by MCPs process.

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Mitochondrial Fission: Regulation and ER Connection

  • Lee, Hakjoo;Yoon, Yisang
    • Molecules and Cells
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    • v.37 no.2
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    • pp.89-94
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    • 2014
  • Fission and fusion of mitochondrial tubules are the main processes determining mitochondrial shape and size in cells. As more evidence is found for the involvement of mitochondrial morphology in human pathology, it is important to elucidate the mechanisms of mitochondrial fission and fusion. Mitochondrial morphology is highly sensitive to changing environmental conditions, indicating the involvement of cellular signaling pathways. In addition, the well-established structural connection between the endoplasmic reticulum (ER) and mitochondria has recently been found to play a role in mitochondrial fission. This minireview describes the latest advancements in understanding the regulatory mechanisms controlling mitochondrial morphology, as well as the ER-mediated structural maintenance of mitochondria, with a specific emphasis on mitochondrial fission.

A STUDY ON DTCNN APPLYING FUZZY MORPHOLOGY OPERATORS (퍼지 형태학 연산자를 적용한 DTCNN 연구)

  • 변오성;문성룡
    • Proceedings of the IEEK Conference
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    • pp.13-16
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    • 2000
  • This paper is to compare DTCNN(Discrete-time Cellular Neural Networks) applying the fuzzy morphology operators with the conventional FCNN(Fuzzy CNN) using the general morphology operators. These methods are to the image filtering, and are compared as MSE. Also the main goal of this paper is to compare the fuzzy morphology operators with the general morphology operators through image input. In a result of computer simulation, we could know that the error of DTCNN applying the fuzzy morphology operators is less about 6.1809 than FCNN using the general morphology operators in the image included 10% noise, also the error of the former is less about 5.5922 than the latter in the image included 20% noise. And the image of DTCNN applying the fuzzy morphology operators is superior to FCNN using the general morphology operators.

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The Change of Major Cellular Fatty Acids Composition and Morphology of Escherichia coli Affected by Toxic Substances

  • Jeong In Suk;Seong Hee Kyung;Lee Won Jae
    • Biomedical Science Letters
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    • v.10 no.3
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    • pp.293-298
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    • 2004
  • This study was performed to compare the growth rates, cellular fatty acid compositions and morphology by using electron microscope of Escherichia coli (E. coli) grown in various conditions including different concentrations of phenol, CdCl₂ and HgCl₂. Ninety eight E. coli strains were isolated from Naktong river and human feces. The content of unsaturated fatty acids, especially 16:1ω7c and 18:1ωc increased as the concentration of phenol and CdCl₂ increased. The content of unsaturated fatty acid increased up to 50 ppb of HgCl₂, but decreased at 75 ppb of HgCl₂. There were more unsaturated fatty acids than saturated fatty acid in the presence of toxic substances. However, the ration was reversed when the affected E. coli was transferred to toxic substance free fresh trypticase soy broth medium. Also, by using transmission electron microscope these cells were observed to various morphological deformation by heavy metals and their deposition on the surface. From these results, we suggested that the changes of major fatty acids composition and morphology of E. coli may be considered to indicate contaminated levels of heavy metals or organic solvents. The information presented here may be useful in predicting effects of heavy-metal and organic solvent contamination in streams and provides a basis for further studies of metal or organic solvent effects on microbial communities.

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Cytotoxicity of Nigella Sativa Seed Oil and Extract Against Human Lung Cancer Cell Line

  • Al-Sheddi, Ebtesam Saad;Farshori, Nida Nayyar;Al-Oqail, Mai Mohammad;Musarrat, Javed;Al-Khedhairy, Abdulaziz Ali;Siddiqui, Maqsood Ahmed
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.2
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    • pp.983-987
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    • 2014
  • Nigella sativa (N sativa), commonly known as black seed, has been used in traditional medicine to treat many diseases. The antioxidant, anti-inflammatory, and antibacterial activities of N sativa extracts are well known. Therefore, the present study was designed to investigate the anticancer activity of seed extract (NSE) and seed oil (NSO) of N sativa against a human lung cancer cell line. Cells were exposed to 0.01 to 1 mg/ml of NSE and NSO for 24 h, then percent cell viability was assessed by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed NSE and NSO significantly reduce the cell viability and alter the cellular morphology of A-549 cells in a concentration dependent manner. The percent cell viability was recorded as 75%, 50%, and 26% at 0.25, 0.5, and 1 mg/ml of NSE by MTT assay and 73%, 48%, and 23% at 0.25, 0.5, and 1 mg/ml of NSE by NRU assay. Exposure to NSO concentrations of 0.1 mg/ml and above for 24 h was also found to be cytotoxic. The decrease in cell viability at 0.1, 0.25, 0.5, and 1 mg/ml of NSO was recorded to be 89%, 52%, 41%, and 13% by MTT assay and 85%, 52%, 38%, and 11% by NRU assay, respectively. A-549 cells exposed to 0.25, 0.5 and 1 mg/ml of NSE and NSO lost their typical morphology and appeared smaller in size. The data revealed that the treatment of seed extract (NSE) and seed oil (NSO) of Nigella sativa significantly reduce viability of human lung cancer cells.

Anticancer Activity of Petroselinum sativum Seed Extracts on MCF-7 Human Breast Cancer Cells

  • Farshori, Nida Nayyar;Al-Sheddi, Ebtesam Saad;Al-Oqail, Mai Mohammad;Musarrat, Javed;Al-Khedhairy, Abdulaziz Ali;Siddiqui, Maqsood Ahmed
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.5719-5723
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    • 2013
  • Pharmacological and preventive properties of Petroselinum sativum seed extracts are well known, but the anticancer activity of alcoholic extracts and oil of Petroselinum sativum seeds on human breast cancer cells have not been explored so far. Therefore, the present study was designed to investigate the cytotoxic activities of these extracts against MCF-7 cells. Cells were exposed to 10 to $1000{\mu}g/ml$ of alcoholic seed extract (PSA) and seed oil (PSO) of Petroselinum sativum for 24 h. Post-treatment, percent cell viability was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) and neutral red uptake (NRU) assays, and cellular morphology by phase contrast inverted microscopy. The results showed that PSA and PSO significantly reduced cell viability, and altered the cellular morphology of MCF-7 cells in a concentration dependent manner. Concentrations of $50{\mu}g/ml$ and above of PSA and $100{\mu}g/ml$ and above of PSO were found to be cytotoxic in MCF-7 cells. Cell viability at 50, 100, 250, 500 and $1000{\mu}g/ml$ of PSA was recorded as 81%, 57%, 33%, 8% and 5%, respectively, whereas at 100, 250, 500, and $1000{\mu}g/ml$ of PSO values were 90%, 78%, 62%, and 8%, respectively by MTT assay. MCF-7 cells exposed to 250, 500 and $1000{\mu}g/ml$ of PSA and PSO lost their typical morphology and appeared smaller in size. The data revealed that the treatment with PSA and PSO of Petroselinum sativum induced cell death in MCF-7 cells.