• Title, Summary, Keyword: cell migration

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Inhibitory Effects on Human Breast Cancer Cells Migration of Small Black Bean according to the Cooking Methods (조리방법을 달리한 쥐눈이콩의 인체유방암세포 이동성 억제 효과)

  • Shin, Jihun;Joo, Nami
    • The Korean Journal of Food And Nutrition
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    • v.30 no.4
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    • pp.728-734
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    • 2017
  • After being subjected to different cooking methods, small black beans (Rhynchosia nulubilis) were investigated in order to assess the effects of the retained bioactive compounds. Using uncooked, pan broiled, boiled, steamed, and pressure cooked beans, the inhibitory effects of MCF-7 cell migration were evaluated at protein concentrations of 40, 160, and $640{\mu}m/mL$, using the Boyden's chamber assay. All protein concentrations (40, 160, and $640{\mu}m/mL$) of pan broiled beans showed significant reduction (59.83, 32.48, and 21.37%, respectively) in the rate of cell migration to the lower chambers (p-value less than 0.001). Estimated cell migration rates correlated to the exponential decay between experimentally measured cell migration rates and converted samples. The range of estimated cell migration rate for each 100 mg/mL of cooked sample was as follows: pan broiled (21.16%), boiled (22.48%), steamed (22.48%), pressure cooked (29.52%), and uncooked (35.03%) beans. Our study indicated that selective modifications of cooking methods for small black beans, such as pan broiling, ameliorated the inhibitory effects of MCF-7 cell migration. This suggests that optimized cooking methods increase the nutritional contents of the cooked food.

miR-485 Acts as a Tumor Suppressor by Inhibiting Cell Growth and Migration in Breast Carcinoma T47D Cells

  • Anaya-Ruiz, Maricruz;Bandala, Cindy;Perez-Santos, Jose Luis Martin
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3757-3760
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    • 2013
  • MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells. Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR-485 might play an important role in breast cancer by suppressing cell proliferation and migration.

Synthesis of Biodegradable Polymers and Measurement of Cell Attachment and Migration Rates (생분해성 고분자의 합성 및 세포부착과 침입속도의 측정)

  • 이찬우
    • Textile Science and Engineering
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    • v.40 no.5
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    • pp.401-407
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    • 2003
  • Various biodegradable polymers (PLLA-PEG-PLLA, PLLA-PPG-PLLA, PLLA-PN-PLLA) were synthesized and their affinities with cells were measured. Cell migration rate was more dependent on the cell density rather than on the characteristics of polymer. PLLA-PN-PLLA film showed formation of cohesive cell structure. The contact angle of this film was almost the same as that of PLLA. However, initial number of cell attachment of PLLA film was 3 to 4 times higher than that of PLLA-PN-PLLA copolymer film.

Cigarette Smoke Extract-induced Reduction in Migration and Contraction in Normal Human Bronchial Smooth Muscle Cells

  • Yoon, Chul-Ho;Park, Hye-Jin;Cho, Young-Woo;Kim, Eun-Jin;Lee, Jong-Deog;Kang, Kee-Ryeon;Han, Jae-Hee;Kang, Da-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.15 no.6
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    • pp.397-403
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    • 2011
  • The proliferation, migration, cytokine release, and contraction of airway smooth muscle cells are key events in the airway remodeling process that occur in lung disease such as asthma, chronic obstruction pulmonary disease, and cancer. These events can be modulated by a number of factors, including cigarette smoke extract (CSE). CSE-induced alterations in the viability, migration, and contractile abilities of normal human airway cells remain unclear. This study investigated the effect of CSE on cell viability, migration, tumor necrosis factor (TNF)-${\alpha}$ secretion, and contraction in normal human bronchial smooth muscle cells (HBSMCs). Treatment of HBSMCs with 10% CSE induced cell death, and the death was accompanied by the generation of reactive oxygen species (ROS). CSE-induced cell death was reduced by N-acetyl-l-cysteine (NAC), an ROS scavenger. In addition, CSE reduced the migration ability of HBSMCs by 75%. The combination of NAC with CSE blocked the CSE-induced reduction of cell migration. However, CSE had no effect on TNF-${\alpha}$ secretion and NF-${\kappa}B$ activation. CSE induced an increase in intracellular $Ca^{2+}$ concentration in 64% of HBSMCs. CSE reduced the contractile ability of HBSMCs, and the ability was enhanced by NAC treatment. These results demonstrate that CSE treatment induces cell death and reduces migration and contraction by increasing ROS generation in normal HBSMCs. These results suggest that CSE may induce airway change through cell death and reduction in migration and contraction of normal HBSMCs.

EphB/ephrinB Signaling in Cell Adhesion and Migration

  • Park, Inji;Lee, Hyun-Shik
    • Molecules and Cells
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    • v.38 no.1
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    • pp.14-19
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    • 2015
  • Eph receptors and their ligands, ephrins, represent the largest group of the receptor tyrosine kinase (RTK) family, and they mediate numerous developmental processes in a variety of organisms. Ephrins are membrane-bound proteins that are mainly divided into two classes: A class ephrins, which are linked to the membrane by a glycosylphosphatidylinositol (GPI) linkage, and B class ephrins, which are transmembrane ligands. Based on their domain structures and affinities for ligand binding, the Eph receptors are also divided into two groups. Trans-dimerization of Eph receptors with their membrane-tethered ligands regulates cell-cell interactions and initiates bidirectional signaling pathways. These pathways are intimately involved in regulating cytoskeleton dynamics, cell migration, and alterations in cellular dynamics and shapes. The EphBs and ephrinBs are specifically localized and modified to promote higher-order clustering and initiate of bidirectional signaling. In this review, we present an in-depth overview of the structure, mechanisms, cell signaling, and functions of EphB/ephrinB in cell adhesion and migration.

Visualization and Velocity Measurement of Migrating Cells in Microchannels with Various Width (다양한 크기의 마이크로 채널 내에서 이동하는 세포의 가시화 및 이동 속도 측정)

  • Lee, Seung-Youl;Kim, Myung-Jun;Chae, Sang-Min;Jin, Song-Wan
    • Journal of the Korean Society of Visualization
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    • v.9 no.1
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    • pp.29-35
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    • 2011
  • In this paper, we applied various microfluidic chambers to visualize cell migration and measured the migration velocity in microchannels. Migration speed of B16 cell in a large channel was similar with that of wound healing experiment. However, the speed decreased gradually as the cell move inside of the channel. It is expected that this phenomenon is due to the shortage of oxygen or nutrition, whichare essential for cell growth. In the case of cell in the small channel whose size is smaller than the cell, its speed was slower than that in a larger channel.

The Effects of Various Extracellular Matrices on Motility of Cultured MC3T3-E1 Cell (다양한 세포외기질이 배양 골아세포의 이동에 미치는 영향)

  • Park, Beyoung Yun;Seo, Sang Woo;Lee, Won Jai;Ryu, Chang Woo;Rah, Dong Kyun;Son, Hyun Joo;Park, Jong Chul
    • Archives of Plastic Surgery
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    • v.32 no.2
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    • pp.143-148
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    • 2005
  • Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on $100{\mu}l/ml$ laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.

Chemotactic Cell Migration around Hollow Silica Beads Containing Chemotatic Reagent (약물 담지 다공성 중공 실리카 미세구 주위 세포의 주화성 이동)

  • Kim, Hae-Chun;Kang, Mi-Seon;Rhee, Seog-Woo
    • KSBB Journal
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    • v.25 no.4
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    • pp.344-350
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    • 2010
  • This paper demonstrates a microfluidic chip incorporating patterned hollow silica beads that can be effectively used for chemotaxis assay. The hollow silica bead has been exploited to develop a carrier for chemoattractant to induce cell migration. The microfluidic chip contains a patterned array of microfabricated docks which can hold only one bead per docking site. The hollow bead placed inside microfluidic chip releases chemotactic reagent (PDGF-BB) around its periphery in a controlled fashion which generates a signal for chemotatic migration of fibroblast cells. The number of cells migrated close to each bead has been assessed. On-chip cell migration assay showed a remarkable result proving the high efficiency and reliable accuracy in quantitative analysis. Therefore, the device could be extensively used in cell migration assay and other various studies related to cellular movements.

Inhibition of The Stem Cell Factor-Induced Migration of Mast Cells by Dexamethasone

  • Jeong, Hyun-Ja;Hong, Seung-Heon;Park, Rae-Kil;Kim, Hyung-Min
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.76-76
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    • 2003
  • Mast cells accumulation can be causally related with several allergic inflammations. Previous work has demonstrated that glucocorticoids decreased tissue mast cell number and stem cell factor (SCF)-induced migration of mast cells required p38 mitogen-activated protein kinase (MAPK) activation. In the present study, we investigated the effects of dexamethasone on SCF-induced migration of rat peritoneal mast cells (RPMCs). SCF significantly induced migration of RPMCs at 4 h. Dexamethasone dose-dependently inhibited SCF-induced migration of RPMCs (about 90.1% at 100 nM, P<0.05). MAPK p38 inhibitor, SB203580 (20 ${\mu}$M) also inhibited the SCF-induced migration. The ability of SCF to enhance morphological alteration and F -actin formation was also abolished by treatment of dexamethasone. Dexamethasone inhibited SCF-induced p38 MAPK activation to near basal level and induced the MKP-1 expression. In addition, SCF-induced inflammatory cytokine production was significantly inhibited by treatment of dexamethasone or SB203580 (p<0.01). Our results show that dexamethasone potently regulates SCF -induced migration, p38 MAPK activation and inflammatory cytokine production through expression of MKP-l protein in RPMCs. Such modulation may have functional consequences during dexamethasone treatment, especially mast cell-mediated allergic inflammation disorders.

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