• Title, Summary, Keyword: blood corticosterone

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Effects of Glycyrrhizae Radix on Serum Corticosterone and Blood Histamine Content by Immobilization Stress in Mice (감초(甘草) 엑기스가 Immobilization Stress 부하(負荷)후 혈중(血中) Corticosterone 및 Histamine 함량변화(含量變化)에 미치는 영향)

  • Eun, Jae-Soon;Oh, Chan-Ho;Han, Jong-Hyun
    • Korean Journal of Pharmacognosy
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    • v.20 no.1
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    • pp.37-42
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    • 1989
  • This study was carried out to investigate the effect of Glycyrrhizae Radix on serum corticosterone and blood histamine content by immobilization stress in mice. Corticosterone secretion and blood histamine level was significantly increased in mice by subjecting the animals to immobilization stress 1 hr. after intraperitoneal injection of Glycyrrhizae Radix extract (150 mg/kg) and glycyrrhizinic and (15 mg/kg). whereas, administration of cortisol $(7.5\;{\mu}g/kg)$ provoked a decrease in corticosterone secretion and histamine levels. These results suggested that glycyrrhizinic acid was effective on cortiosterone release provoked by immobilization stress and this release was mediated in part by histamine.

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Influence of Chlordiazepoxide on the Changes of Blood Sugar and Plasma Corticosterone Level Induced by ACTH and Picrotoxin in Mice (ACTH와 Picrotoxin에 의(依)한 혈당(血糖)과 혈장(血漿) Corticosterone 치(値)의 변동(變動)에 미치는 Chlordiazepoxide의 영향(影響))

  • Kim, Young-Gyu;Shin, Man-Ryun
    • The Korean Journal of Pharmacology
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    • v.16 no.2
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    • pp.39-44
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    • 1980
  • Marc et al. reported that diazepam increased plasma corticosterone level and Dasgupta et al. suggested that chlordiazepoxide(CDP) supressed the adrenal response to ACTH. In this paper, the influence of CDP on the changes of blood sugar and plasma corticosterone level induced by ACTH and picrotoxin were investigated in male mice. The results obtained were summarized as follows; 1) The blood sugar and plasma corticosterone level were increased by CDP, ACTH, and picrotoxin, respectively. 2) The hyperglycemia induced by ACTH and picrotoxin were not affected by the CDP pretreatment. 3) The increase of plasma corticosterone level induced by ACTH was inhibited by the CDP pretreatment. 4) The increase of plasma corticosterone level appeared 30 minutes after picrotoxin injection was slightly enhanced, but the level of 120 minutes after picrotoxin injection was significantly inhibited by the CDP pretreatment.

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Influence of Phenytoin and Phenobarbital on the Changes of Brain Norepinephrine Content and Plasma Corticosterone Level in Mice (Phenytoin과 Phenobarbital이 뇌내(腦內) Catecholamine함량(含量)과 혈장(血漿) Corticosterone치(値) 변동(變動)에 미치는 영향(影響))

  • Kim, Nam-Heon;Cheon, Yun-Sook
    • The Korean Journal of Pharmacology
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    • v.18 no.2
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    • pp.51-58
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    • 1982
  • In this paper, the influence of phenytoin and phenobarbical on the changes of brain norepinephrine(NE) content, plasma corticosterone and blood sugar level in mice were studied. The results obtained were summarized as follows: 1) Phenytoin(50 mg/kg) increased the brain NE content but phenobarbital(50 mg/kg) did not affect. The increase of the brain NE content induced phenytoin was potentiated by phenobarbital pretreatment. 2) Phenytoin(25 mg/kg, 50 mg/kg) markedly increased the level of plasma corticosterone but phenobarbital did not affect. The increase of the plasma corticosterone induced by phenytoin was inhibited by phenobarbital pretreatment. 3) Phenytoin(50 mg/kg) markedly increased the blood sugar level but phenobarbital did not affect. The increase of the blood sugar induced by phenytoin was not affected by phenobarbital pretreatment.

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Effect of pertussis toxin pretreated centrally on blood glucose level induced by stress

  • Suh, Hong-Won;Sim, Yun-Beom;Park, Soo-Hyun;Sharma, Naveen;Im, Hyun-Ju;Hong, Jae-Seung
    • The Korean Journal of Physiology and Pharmacology
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    • v.20 no.5
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    • pp.467-476
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    • 2016
  • In the present study, we examined the effect of pertussis toxin (PTX) administered centrally in a variety of stress-induced blood glucose level. Mice were exposed to stress after the pretreatment of PTX (0.05 or 0.1 mg) i.c.v. or i.t. once for 6 days. Blood glucose level was measured at 0, 30, 60 and 120 min after stress stimulation. The blood glucose level was increased in all stress groups. The blood glucose level reached at maximum level after 30 min of stress stimulation and returned to a normal level after 2 h of stress stimulation in restraint stress, physical, and emotional stress groups. The blood glucose level induced by cold-water swimming stress was gradually increased up to 1 h and returned to the normal level. The intracerebroventricular (i.c.v.) or intrathecal (i.t.) pretreatment with PTX, a $G_i$ inhibitor, alone produced a hypoglycemia and almost abolished the elevation of the blood level induced by stress stimulation. The central pretreatment with PTX caused a reduction of plasma insulin level, whereas plasma corticosterone level was further up-regulated in all stress models. Our results suggest that the hyperglycemia produced by physical stress, emotional stress, restraint stress, and the cold-water swimming stress appear to be mediated by activation of centrally located PTX-sensitive G proteins. The reduction of blood glucose level by PTX appears to due to the reduction of plasma insulin level. The reduction of blood glucose level by PTX was accompanied by the reduction of plasma insulin level. Plasma corticosterone level up-regulation by PTX in stress models may be due to a blood glucose homeostatic mechanism.

Anti-stress Effect of Scutellatia baicalensis in SD Rats and ICR Mice

  • Ryu, Jong-Hoon;Tan-Lee, Blendyl Saguan;Jung, Ji-Woong;Ahn, Nam-Yoon;Lee, Seung-Joo;Yu, Gu-Young;Han, Shin-Ha;Lee, Jeong-Hoon;Lee, Geum-Seon;Cheong, Jae-Hoon
    • Biomolecules & Therapeutics
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    • v.12 no.1
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    • pp.34-42
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    • 2004
  • The aim of this study is to investigate anti-stress effect of Scutellaria baicalensis(SB). The experiments were performed with the use of young (9 weeks of age) male rats of SD strain and the male ICR mice (20-25 g) at the time of first treatment with SB. Animals of the normal group were not exposed to any stress and the control group were exposed to stress. The rats of the Ginseng, Diazepam(BZ) and SB supplementary group were orally administered once a day 100 mg of red ginseng extract, 5 mg of BZ or 100 mg of SB extract/kg body weight and they were exposed to stress. The mice of the Ginseng, BZ and SB supplementary group were given water containing 200 mg of red ginseng extract, 10 mg of BZ or SB extract/100 ml potable water and exposed to stress. Animals were given supplements for 7 days without stress, and then were given supplement for 5 days with restraining and electroshock stress. We recorded stress related behavioral changes of the experimental animals by stressing them using the Etho-vision system and measured levels of blood corticosterone and IL-2. SB supplementation partially blocked the stress effect on locomotion in the rats and mice, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, grooming, smelling, and rearing behavior in the rats and smelling, grooming, tailing, and rearing in the mice. in elevated plus maze test, the staying time of the stressed rats and mice in the open area decreased while it increased in the closed area. But these changes also partially were blocked by SB-supplementation. SB-supplementation decreased levels of the blood corticosterone which was increased by stress in the rats but did not significantly increase levels of blood interleukin 2 which was decreased by stress in mice.

Effect of Atractylodis Macrocephalae Rhizoma on Starvation Stress in Mice (백출이 생쥐의 기아 Stress에 미치는 영향)

  • Park Jung-Chul;Song Yun-Kyung;Lim Hyung-Ho
    • The Journal of Korean Medicine
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    • v.25 no.3
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    • pp.55-66
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    • 2004
  • Objectives : This study was aimed to evaluate the anti-starvation stress effect of Atractylodis Macrocephalae Rhizoma on mice. Methods : First, we divided the mice into 6 groups: Normal Group (group with no starvation), Control Group (administered normal saline 6 times before starting 36 hours starvation), Sample A Group (administered Atractylodis Macrocephalae Rhizoma 0.25g/kg 6 times before starting 36 hours starvation), Sample B Group (administered Atractylodis Mocrocephalae Rhizoma 0.5g/kg 6 times before starting 36 hours starvation), Sample C Group (administered Atractylodis Macrocephalae Rhizoma 1.0g/kg 6 times before starting 36 hours starvation), and Sample D Group (administered Atractylodis Macrocephalae Rhizoma 3.0g/kg 6 times before starting 36 hours starvation). Blood was collected from the retro-orbital plexus and then we measured the plasma corticosterone level from the blood. Rectal temperature was measured right after the blood collection. Results : 1. The plasma corticosterone level in Sample A. B, C, and D Groups decreased compared with the Control Group. Sample A and Sample B Groups showed significant differences (p<0.05, p<0.01) compared with the Control Group. 2. The rectal temperature in Sample A, B, C, and D Groups increased compared with the Control Group. Sample A, B. C, and D Groups showed significant differences (p<0.001, p<0.001, p<0.01, p<0.001) compared with the Control Group. Conclusions : Based on the above results, it might be recognized that Atractylodis Macrocephalae Rhizoma has anti-starvation stress effect, and that further study is needed from various viewpoints.

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Anti-Stress Effects of Ginseng in Immobilization-Stressed Rats

  • Choi, Eun-Ha;Lee, Hyun-Jung;Kim, Cheol-Jin;Kim, Jong-Tae;Kwun, In-Sook;Kim, Yang-Ha
    • Preventive Nutrition and Food Science
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    • v.9 no.3
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    • pp.253-258
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    • 2004
  • Stress is a global menace exacerbated by the advancement of industrialization. Failure of stress management is to a breakdown of the psychological and physiological protection mechanisms against stress. The aim of present study was to investigate the anti-stress potential of ginseng against immobilization stress. Male Sprague-Dawley rats (n=24) were divided into three groups; (i) control, (ii) immobilization stress (2hr daily, for 2 weeks), and (iii) immobilization stress (2 hr daily, for 2 weeks) plus oral administration of ginseng (200 mg/kg BW Id). Immobilization stress resulted in a significant inhibition of body weight gain by 45 % and a significant decrease in the tissue weights of thymus and spleen (p < 0.05). The concentrations of blood GOT and GPT were significantly increased in the immobilization-stressed group compared to the control group (p < 0.05). There were no differences in the blood cholesterol levels among groups. Ginseng administration in the immobilization-stressed group tended to reverse the lack of body weight gain and food intake, though not significantly. The ginseng-administered group showed a significant reversal in the stress-induced effect on spleen and thymus weight, increasing the tissue weights by 16% and 20%, respectively, compared to immobilization-stressed group (p<0.05). The plasma corticosterone level was significantly increased in the stressed group by 39 % compared to the control group (p<0.05), but ginseng administration significantly reversed the stress-induced increase in plasma corticosterone by 15 % compared to the immobilization-stressed group. The present study suggests that the anti-stress effect of ginseng is mediated by normalization of stress-induced changes in the circulating hormones and a reversal of tissue weight loss, thereby returning the body to normal homeostasis.

Effect of Sulfonylureas Administered Centrally on the Blood Glucose Level in Immobilization Stress Model

  • Sharma, Naveen;Sim, Yun-Beom;Park, Soo-Hyun;Lim, Su-Min;Kim, Sung-Su;Jung, Jun-Sub;Hong, Jae-Seung;Suh, Hong-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.3
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    • pp.197-202
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    • 2015
  • Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with $30{\mu}g$ of glyburide, glipizide, glimepiride or tolazamide attenuated the increased blood glucose level induced by immobilization stress. Immobilization stress causes an elevation of the blood corticosterone and insulin levels. Sulfonylureas pretreated i.c.v. caused a further elevation of the blood corticosterone level when mice were forced into the stress. In addition, sulfonylureas pretreated i.c.v. alone caused an elevation of the plasma insulin level. Furthermore, immobilization stress-induced insulin level was reduced by i.c.v. pretreated sulfonylureas. Our results suggest that lowering effect of sulfonylureas administered supraspinally against immobilization stress-induced increase of the blood glucose level appears to be primarily mediated via elevation of the plasma insulin level.

The Effect of Samul-tanggahyangbuja on Depression and Learning on Repeated Stress in Ovariectomized Rats (사물탕가향부자(四物湯加香附子)가 난소적출 흰쥐의 우울 및 학습에 미치는 영향)

  • Lee, Soon-Yee;Kim, Song-Baek;Seo, Yun-Jung;Choi, Chang-Min;Cho, Han-Baek
    • The Journal of Korean Obstetrics and Gynecology
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    • v.26 no.3
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    • pp.1-17
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    • 2013
  • Objectives: In this research, the effect of samul-tanggahyangbuja on depression and learning in ovariectomized rats subjected to repetitive stress were assessed. Samul-tanggahyangbuja is the prescription consisting of Samul-tang and Cyperi Rhizoma. Methods: Ovariectomized rats were repeatedly stressed over a 2-week period. After being orally medicated with samul-tanggahyangbuja (100 or 400 mg/kg), rats performed the Morris water maze test and forced swimming test, and social exploration was assessed in a behavior test. As well, sucrose intake was measured and measurements of blood serum corticosterone and the change of interleukin-$1{\beta}$ (IL-$1{\beta}$) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in blood samples were made. Results: 1. In the Morris water maze test, rats medicated with 100 mg samul-tanggahyangbuja mastered the maze in a shorter time on the 4th day in comparison with the control group, while rats medicated with 400 mg samul-tanggahyangbuja mastered the maze more quickly (p<0.05 on the 3rd day ; p<0.01 on the 4th day, as compared to control). 2. Immobility time in the forced swimming test was significantly decreased in rats receiving 400 mg samul-tanggahyangbuja compared with the control group (p<0.05). 3. Sucrose intake and active social behavior of rats receiving 400 mg samul-tanggahyangbuja were markedly increased in comparison with the control group (p<0.01). 4. Blood serum corticosterone measurements revealed decreased blood serum corticosterone level after medicating with samul-tanggahyangbuja. But it was not statistically significant. 5. Treatment with either dose of samul-tanggahyangbuja significantly reduced IL-$1{\beta}$ and TNF-${\alpha}$ (p<0.05). Conclusions: These results suggest that samul-tanggahyangbuja possesses the anti-depressant and cognitive-enhancing activities related to menopause.

Anti-depressant Effect of Chilbokum under the Forced Swimming Test in Rats

  • Shim, In-Sop;Kim, Jung-Ki;Koo, Byung-Soo
    • The Journal of Korean Medicine
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    • v.27 no.4
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    • pp.191-197
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    • 2006
  • Objectives : The aim of this study was to evaluate the anti-depressant effect of Chilbokum on rats under the forced swimming test (FST) Methods : The rats were treated with the herbal extract, Chilbokum. In order to induce depression-like symptoms, the FST was conducted afterwards. The immobility time was measured during a 5-min experimental session. The alterations of the 5-HT level in the hypothalamus and hippocampus and the change of corticosterone level in the blood induced by FST were determined in the rats. Results : The results were as follows: 1. The immobility time during 5 min of FST in the drug administration group showed significant decreases compared with the control group (p<0.05). 2. The FST+Chilbokum group had significantly increased 5-HT levels of the hypothalamus and hippocampus, compared with the control group (p<0.05, respectively). 3. The FST+Chilbokum group had significantly decreased corticosterone levels, compared with the control group (p<0.05). Conclusions : These results demonstrate that the reduced immobility time by Chilbokum may be mediated by the increase in 5-HT level in the hypothalamus and hippocampus, suggesting that Chilbokum has a potential therapeutic efficacy for human depression.

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