• Title, Summary, Keyword: biosensor

Search Result 643, Processing Time 0.037 seconds

Introduction of Various Amine Groups onto Poly(glycidyl methacrylate)-g-MWNTs and their Application as Biosensor Supports (폴리(글리시딜 메타크릴레이트)가 그래프트된 다중벽 탄소나노튜브에 다양한 아민 그룹의 도입과 바이오센서 지지체로서의 응용)

  • Chung, Da-Jung;Kim, Ki-Chul;Choi, Seong-Ho
    • Polymer Korea
    • /
    • v.36 no.4
    • /
    • pp.470-477
    • /
    • 2012
  • A tyrosinase-immobilized biosensor was developed based on various amine-modified multi-walled carbon nanotube (MWNT) supports for the detection of phenolic compounds. MWNTs with various amine groups were prepared by radiation-induced graft polymerization of glycidyl methacrylate (GMA) onto MWNT supports and the subsequent amination of poly(GMA) graft chains. The physical and chemical properties of the poly(GMA)-grafted MWNT supports and the aminated MWNT supports were investigated by SEM, XPS, and TGA. Furthermore, the electrochemical properties of the prepared tyrosinase-modified biosensor based on MWNT supports with amine groups were also investigated. The response of the enzymatic biosensor was in the range of 0.1-0.9 mM for the concentration of phenol in a phosphate buffer solution. Various parameters influencing biosensor performance have been optimized: binder effects, pH, temperature, and the response to various phenolic compounds. The biosensor was tested on phenolic compounds contained in two different commercial red wines.

Implementation of Biosensor Pattern Using Micro Patterning Technique (미세전극 패터닝 기술을 이용한 바이오센서 패턴 구현)

  • Ko, Jeong Beom;Kim, Hyung Chan;Yang, Young Jin;Kim, Hyun Bum;Yang, Seong Wook;Oh, Seung Ho;Doh, Yang Hoi;Choi, Kyung Hyun
    • Journal of the Korean Society of Manufacturing Process Engineers
    • /
    • v.15 no.6
    • /
    • pp.122-128
    • /
    • 2016
  • The Biosensor biosensor pattern was developed by via an EHD (electro-hydro-dynamics (EHD) patterning process that was performed under atmospheric pressure at room temperature in a single step. The drop diameter was smaller than nozzle diameter and applied high viscosity conductive ink was applied in the EHD patterning method to provide a clear advantage over the piezo and thermal inkjet printing techniques. The Biosensor's biosensor's micro electrode pattern was printed by via a continuous EHD patterning method using 3three- type types of control parameters parameter (input voltage, patterning speed, nozzle pressure). High viscosity (1000 cps) conductive ink with 75 wt% of silver nanoparticles was used for experimentation. The incremental result of impedance of biosensor impedance was measured between the antibody ($10ug{\mu}g/ml$) to spore (0.1 ng/ml, 10 ng/ml, and $1ug{\mu}g./ml$) reaction at frequency 493 MHz frequency.

Recognition of Microorganisms Using SPR Biosensor Immobilized with Thiolated Antibody (티올화 항체고정형 SPR 바이오센서를 이용한 미생물 인식)

  • 조용진;김남수
    • Journal of Biosystems Engineering
    • /
    • v.28 no.2
    • /
    • pp.167-172
    • /
    • 2003
  • This study was performed to fabricate a batch-type SPR biosensing system using a thiolated E. coli antibody coupling, and to explore the feasibility of real-time detection of E. coii in a stagnant sample solution. In advance. “O” and “K” antigenic serotype E. coli antibodies were thiolated with sulfo-LC-SPDP and dithiothreitol, and immobilized by chemisorption in the gold surface of compact SPR sensors. When the SPR biosensor immobilized with E. coli antibody monitored a E. coli solution, it took 3 to 5 min to stabilize. The SPR biosensing system developed in this study was able to detect E. coli in the range above 10$^4$ CFU/mL at the 0.05 significant level. Also, the SPR biosensor had possibility to significantly detect E. coli in the range of 10$^2$ to 10$^4$ CFU/mL in E. coli solutions. Meanwhile, when the SPR biosensor immobilized with 5. coli antibody was cleaned with NaOH solutions, its ability to detect E. coli largely decreased due to wash-out of the immobilized antibody. In order to reuse the SPR sensor, it should be antibody-immobilized newly.

A Study on Dip-Pen Nanolithography Process to fabricate Two-dimensional Photonic Crystal for Planar-type Optical Biosensor (평판형 광-바이오센서용 2차원 광자결정 제작을 위한 Dip-Pen Nanolithography 공정 연구)

  • Kim Jun-Hyong;Lee Jong-Il;Lee Hyun-Yong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.19 no.3
    • /
    • pp.267-272
    • /
    • 2006
  • Optical waveguide based on symmetric and asymmetric Mach-Zehnder interferometer(MZI) type was designed, fabricated and measured the optical characteristics for the application of biosensor. The wavelength of the input optical signal for the device was 1550 nm. And the difference of refractive index was $0.45\;{\Delta}\%$ between core and cladding of the device. The TM(Transverse Magnetic) mode optical properties of the biosensor were analyzed with the refractive index variation of gold thin film deposited for overclad. Nowadays, nano-photonic crystal structures have been paied much attention for its high optical sensitivity. There is a technique to realize the structure, which is called Dip-Pen Nanolithography(DPN) process. The process requires a nano-scale process patterning resolution and high reliability. In this paper, two dimensional nano-photonic crystal array on the surface was proposed for improving the sensitivity of optical biosensor. And the Dip-Pen Nanolithogrphy process was investigated to realize it.

Detection of Pathogenic Salmonella Using a Surface Plasmon Resonance Biosensor (표면플라즈몬공명 바이오센서를 이용한 살모넬라 검출)

  • Cho, Han-Keun;Kim, Gi-Young;Kim, Woon-Ho;Sung, Min-Sun
    • Journal of Biosystems Engineering
    • /
    • v.35 no.2
    • /
    • pp.116-123
    • /
    • 2010
  • Rapid detection of foodborne pathogens has been a major challenge for the food industry. Salmonella contamination is well known in all foods including pasteurised milk. The possibility of specific detection of Salmonella Enteritidis by surface plasmon resonance (SPR) biosensor was explored using a commercially available portable SPR sensor. Self assembly technique was adopted to immobilize anti-Salmonella antibodies on the gold sensing surface of the SPR sensor. The concentration of polyclonal antibody for use in the SPR biosensor was chosen to 1.0 mg/mL. Experiments were conducted at near real-time with results obtained for one SPR biosensor assay within 1 hour. The limit of detection for Salmonella Enteritidis was determined to be $10^6$ CFU/mL in both PBS buffer and milk samples. The assay sensitivity was not significantly affected by milk matrix. Our results showed that it would be possible for employing the SPR biosensor to detect Salmonella Enteritidis in near real-time.