• Title, Summary, Keyword: biosensor

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Tyrosinase-Immobilized Biosensor Based on Ionic Property-Modified MWNTs Prepared by Radiation-Induced Graft Polymerization

  • Ryu, Ha-Na;Choi, Seong-Ho
    • Carbon letters
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    • v.11 no.3
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    • pp.216-223
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    • 2010
  • Two-types of ionically modified multi-walled carbon nanotube (MWNTs) based sensors were developed by radiationinduced graft polymerization using vinyl monomers such as 3-(butyl imidazol)-2-(hydroxyl)propyl methyl methacrylate and 1-[(4-ethenylphenyl)methyl]-3-buthyl-imidazolium chloride with ionic properties, in aqueous solution at room temperature. Subsequently, the tyrosinase-immobilized biosensor was fabricated by a hand-casting of the ionic property-modified MWNTs, tyrosinase, and chitosan solution as a binder onto ITO glass surface. The sensing ranges of the tyrosinase-biosensor for phenol in phosphate buffer solution was in the range of 0.005~0.2 mM. The total phenolic compounds mainly such as caffeine of the tyrosinase-immobilized biosensor for commercial coffee were also determined.

Development of CMOS Image Monitoring System for Measurement of Biosensor Activity using Genetic Algorithm (유전자 알고리즘을 이용한 바이오센서 활동량 측정 CMOS 이미지 센서 모니터링 시스템 개발)

  • Park, Se-Hyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.5
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    • pp.930-936
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    • 2008
  • CMOS image monitoring system for optimal measuring the activity of biosensor is developed using genetic algorithm. Most of living organism in water as water flea, fish, etc are frequently used as biological sensor for monitoring the water quality. It is very difficult to measure the activity of biosensor by image sensor because the value of measurement is varied with gathering method of biosensor images. The suggested monitoring system can optimally measures the activity of biosensor by genetic algorithm. The system is implemented with FPGA into the small hardware which is excellent in terms of the price and performance.

Solid medium integrated impedimetric biosensor for detection of microorganisms (미생물 검침을 위한 고체 배지 임피던스 센서)

  • Choi, Ah-Mi;Park, Jae-Sung;Jung, Hyo-Il
    • Proceedings of the KSME Conference
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    • pp.1629-1632
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    • 2008
  • Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time detection of microorganisms is introduced. We fabricated a new impedimetric biosensor by integrating solid media and two plane electrodes attached on two facing sides of an acryl well. During bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid-medium biosensor could monitor bacterial growth in situ with a detection time of ${\sim}4$ hrs. Our data indicate that the solid-medium biosensor is useful for detecting airborne microorganisms, thereby providing a new analytical tool for impedance microbiology.

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A Biosensor for Diagnosis of Swine Sarcoptic Mange (돼지 옴 감염증 진단을 위한 바이오센서 연구)

  • Cho H. K.;Jee C. H.
    • Journal of Biosystems Engineering
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    • v.30 no.5
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    • pp.306-311
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    • 2005
  • In this study, a biosensor was developed to rapidly diagnose the swine sarcoptic mange (Sarcoptes scabies var. suis). The ELISA was modified to reduce the processing time for rapid diagnosis. The biosensor consists of a biological reaction part, and a measurement and control part. The biological reaction part was designed for using micro-pumps and valves for fluid transportation, and the measurement control part composed of a photodiode, a light-emitting diode fur light measurement, and a microcomputer to implement assay A polystyrene covet was used as a reaction chamber. Signal output was read as the rate of change in optical density at 645nm. Eighteen pigs diagnosed with sacroptic mange and 19 control pigs were tested. Fifteen sacbies-infested pigs showed positive results ($83.3\%$ sensitivity). Sixteen control pigs showed negative results ($84.2\%$ specificity). The system could execute a diagnosis cycle in about 45 min. The results suggest that this biosensor is useful for the rapid diagnosis of swine sacroptic mange.

Detection of Fumonisin $B_1$ by a Batch Type Surface Plasmon Resonance Biosensor

  • Cho, Yong-Jin;Chun, Hyang-Sook;Kim, Chul-Jin;Kim, Chong-Tai;Hong, Ji-Young
    • Food Science and Biotechnology
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    • v.14 no.5
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    • pp.698-699
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    • 2005
  • Levels of fumonisins, mycotoxins produced by fungal species, must be accurately and rapidly monitored to ensure food safety. In this study, using surface plasmon resonance sensor, a batch-type biosensor was fabricated to detect fumonisin $B_1$. By applying this biosensor to fumonisin $B_1$ solutions of 0 to 6 ppm, a significant calibration model was developed for measurement. Coefficient of determination in regression analysis for the model was 0.920. Results indicate that detection of fumonisin $B_1$ by surface plasmon resonance biosensor was highly feasible.

Biosensors and their Applications in Food Safety: A Review

  • Yasmin, Jannat;Ahmed, Mohammed Raju;Cho, Byoung-Kwan
    • Journal of Biosystems Engineering
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    • v.41 no.3
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    • pp.240-254
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    • 2016
  • Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

Electrochemical Detection of Self-Assembled Viologen Modified Electrode as Mediator of Glucose Sensor

  • Lee, Dong-Yun;Choi, Won-Suk;Park, Sang-Hyun;Kwon, Young-Soo
    • Journal of Electrical Engineering and Technology
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    • v.4 no.1
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    • pp.106-110
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    • 2009
  • An amperometric glucose biosensor has been developed using viologen derivatives as a charge transfer mediator between a glucose oxidase (GOD) and a gold electrode. A highly stable self-assembled monolayer (SAM) of thiol-based viologen was immobilized onto the gold electrode of a quartz crystal microbalance (QCM) and GOD was immobilized onto the viologen modified electrode. This biosensor response to glucose was evaluated amperometrically in the potential of -300mV. Upon immobilization of the glucose oxidase onto the viologen modified electrode, the biosensor showed rapid response towards glucose. Experimental conditions influencing the biosensor performance, such as pH potential, were optimized and assessed. This biosensor offered excellent electrochemical responses for glucose concentration below ${\mu}$ mol level with high sensitivity and selectivity and short response time. The levels of the RSDs (<5%) for the entire analyses reflected the highly reproducible sensor performance. A linear calibration range between the current and the glucose concentration was obtained up to $4.5{\times}10^{-4}M$. The detection limit was determined to be $3.0{\times}10^{-6}M$.

$H_2O_2$ Detection Property of Glucose Sensor using Self Assembled Viologen Modified Electrode as Mediator (Viologen 유도체를 전하전달체로 이용한 Glucose 센서의 $H_2O_2$ 검출 특성)

  • Lee, Dong-Yun;Choi, Won-Suk;Park, Sang-Hyun;Kwon, Young-Soo
    • Proceedings of the KIEE Conference
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    • pp.86-87
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    • 2007
  • An amperometric glucose biosensor has been developed using viologen derivatives as electron mediator of glucose oxidase (GOD) at Au electrode. Highly stable self assembled monolayer (SAM) of thiol-based viologen is immobilized onto the Au electrode followed byGOD is immobilized onto the viologen modified electrode. This biosensor response to glucose was evaluated amperometrically in the potential of -300 mV. Upon immobilization of glucose oxidase onto the viologen modified-electrode, the biosensor showed rapid response towards glucose. Experimental conditions influencing the biosensor performance such as, pH, potential were optimized and assessed. This biosensor offered an excellent electrochemical response for glucose concentration below ${\mu}mol$ level with high sensitivity and selectivity and short response time. The levels of the RSD's (< 5 %) for the entire analyses reflected the highly reproducible sensor performance. Using the optimized a linear relationship between current and glucose concentration was obtained up to $4.5{\times}10^{-4}$ M. In addition, this biosensor showed well reproducibility and stability.

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Review on Biosensors for Food Safety

  • Kim, Giyoung;Moon, Ji-Hea;Lim, Jongguk;Mo, Changyeun
    • Journal of Biosystems Engineering
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    • v.39 no.2
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    • pp.115-121
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    • 2014
  • Background: Frequent outbreaks of foodborne illness have been increased awareness of food safety. CDC estimates that each year roughly 48 million people gets sick, 128,000 are hospitalized and 3,000 die of foodborne diseases in US. In Korea, 6,058 were hospitalized and 266 incidents were reported in 2012. It is required to develop rapid methods to identify hazard substances in food products for protecting and maintaining safety of the public health. However, conventional methods for pathogens detection and identification involve prolonged multiple enrichment steps. Purpose: This review aims to provide information on biosensors to detect pathogens in food products to enhance food safety. Results: Foodborne outbreaks continue to occur and outbreaks from various food sources have increased the need for simple, rapid, and sensitive methods to detect foodborne pathogens. Conventional methods for foodborne pathogens detection require tremendous amount of labor and time. Biosensors have drawn attentions in recent years because of their ability to detect analytes sensitively and rapidly. Principles along with their advantages and disadvantages of a variety of food safety biosensors including fiber optic biosensor, impedimetric biosensor, surface Plasmon resonance biosensor, and nano biosensor were explained. Also, future trends for the food safety biosensors were discussed.

Development of Electro-Biosensor for the Residual Pesticides using Organic Carbon and Cobalt Phthalocyanine (Cobalt Phthalocyanine 탄소유기 전극을 이용한 농약 잔류량 측정 센서개발)

  • Yu, Young-Hun;Cho, Hyung-Jun;Park, Won-Pyo;Hyun, Hae-Nam
    • Korean Journal of Environmental Agriculture
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    • v.29 no.1
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    • pp.72-76
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    • 2010
  • We have developed the bio-electrode measuring the variance of the amount of acetylcholine affected by residual pesticide. The working electrode of the biosensor was made by combination of cobalt phthalocyanine and carbon organic compounds. The biosensors were constructed by screen-printing method. The principle of working electrode is similar to thiocholine sensor. We have fabricated the biosensor using standard screen printing method. Generally, the biosensor made by printing method formed thick film biosensor. When the electrodes were made by electrochemical cells, the generation of current by the addition of enzyme substrate was inhibited by standard solutions of organo-phosphate pesticides. The detection limit of sensor is about 0.5 $\mu{g}/L$ for carbofuran. We could improve the responsibility of the sensor by controlling the cobalt phthalocyanine and thiocholine concentration ratio. Also we have tested the EPN and Chlorpyrifos pesticides and found that the biosensor is applicable to fast determination of residual pesticides.