• Title, Summary, Keyword: bacteriocin

Search Result 289, Processing Time 0.035 seconds

Properties of a Lactobacillus acidophilus Bacteriocin (Lactobacillus acidophilus Bacteriocin의 성상에 관한 연구)

  • 정영건;안장연;권오진;강주회
    • Microbiology and Biotechnology Letters
    • /
    • v.17 no.2
    • /
    • pp.94-99
    • /
    • 1989
  • Seven gtrains of Lactobacillus produce bacteriocin being active for Lacidophilus. All strains producing bacteriocin were found to be L. acidophilus except with one strain of L. gasseri. The maximum activity of bacteriocin produced by Lactobacillus strains was obtained at a middle or late stage of the log phase, or a early stage of the stationary phase. After the maximum was reached, however, the activity was rapidly decreased. The bacteriocins were inactivated easily by the treatment with proteolytic enzymes but not with nucleolytic enzymes, suggesting that the bacteriocin was proteinaceous. The bacteriocins were different from the other previously reported lactobacillus bacteriocin in their flexibility to the treatment at 10$0^{\circ}C$. Bacteriocins of L. acidophilus ATCC 9857 and 4357 decreased in activity by the treatment with diethylether, presumably the bacteriocin contained of a lipid component. It sums likely that L. acidophilus A4 bacteriocin adsorb to a regularly arrayed layer of the cell wall.

  • PDF

Bacteriocin Produced by Lactobacillus curvatus SE1 Isolated from Kimchi

  • Kim, Sung-Koo;Lee, Eun-Ju;Park, Keun-Young;Jun, Hong-Ki
    • Journal of Microbiology and Biotechnology
    • /
    • v.8 no.6
    • /
    • pp.588-594
    • /
    • 1998
  • Lactic acid bacteria were isolated from Kimchi and screened for bacteriocin production. Strain SE1, identified as Lactobacillus curvatus sp., showed the strongest inhibitory activity against Lactobacillus delbrueckii subsp. delbrueckii. The bacteriocin was inactivated by amyloglucosidase, trypsin, or protease K treatment. However, it maintained its activity under heat treatment at $100^{\circ}C$ for 60 min. The production of the bacteriocin had a growth-related mode and decreased around the early-stationary phase. The optimum temperature for the growth of L. curvatus SE1 was $37^{\circ}C$; however, the optimum temperature for bacteriocin production was $30^{\circ}C$. The bacteriocin activity was decreased by treatment with methanol, butanol, acetone, or chloroform, however, it was not affected by treatment with ethanol, iso-propanol, or cyclohexane. The inhibitory activity of bacteriocin was stable over a wide range of pHs (2 to 11). The bacteriocin from L. curvatus SE1 killed the indicator strain by a bactericidal mode of action. The bacteriocin from L. curvatus SE1 was partially purified by ethanol precipitation and ion exchange chromatography. SDS-polyacrylamide gel electrophoresis was used to determine the molecular weight of the bacteriocin by the bacteriocin activity test. The apparent molecular mass of the bacteriocin produced by L. curvatus SE1 was about 14 kDa.

  • PDF

Characterization of Bacteriocin Production by Lactococcus sp. J-105 Isolated from Kimchi (김치에서 분리한 Lactococcus sp. J-105가 생산하는 Bacteriocin의 특성)

  • 곽규숙;구재관;배경미;전홍기
    • Journal of Life Science
    • /
    • v.9 no.1
    • /
    • pp.111-120
    • /
    • 1999
  • A bacteriocin-producing strain, J-105, was isolated from Kimchi and identified as Lactococcus sp. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For maximum yield of bacteriocin from Lactotoccus sp. J-105, the cell should be harvested at the early stationary phase and temperature, pH and NaCl concentration should be $25^{\circ}C$, pH 8.0 and without the addition of NaCl, respectively. Maltose should be used as a carbon source and organic nitrogen such as polypeptone should be used as a nitrogen source for the best yield. The bacteriocin from isolate was inhibitory against Acetobacter aceti, Bacillus subtilis and several strains of lactic acid bacteria. The bacteriocin of J-105 was sensitive to pepsin, but stable for heat treatment. It was stable even at autoclaving temperature for 15 min.

  • PDF

Inhibition of Clostridium perfringens using Bacteriophages and Bacteriocin Producing Strains

  • Heo, Sunhak;Kim, Min Gon;Kwon, Mirae;Lee, Hee Soo;Kim, Geun-Bae
    • Food Science of Animal Resources
    • /
    • v.38 no.1
    • /
    • pp.88-98
    • /
    • 2018
  • In this study, we isolated and characterized a bacteriocin-producing strain and two bacteriophages (P4, A3), showing antimicrobial effects against Clostridium perfringens, from chicken and swine feces by the spot-on-the lawn antagonism method. The selected strain was identified as Streptococcus hyointestinalis by 16S rRNA gene sequencing. The bacteriocin from the isolated strain exhibited strong inhibitory activity against four strains of C. perfringens and all the tested strains of Listeria monocytogenes, and the bacteriocin were highly heat- and pH-stable even at pH 2, pH 10 and $121^{\circ}C$ for 15 min. We also evaluated the combined effects of the isolated bacteriocin and phages. Combining the phage treatments and bacteriocin resulted in a synergetic effect compared with the phage or the bacteriocin alone. In addition, during the probiotic test, the bacteriocin-producing S. hyointestinalis B19 strain reduced the population of C. perfringens significantly. Treatment with S. hyointestinalis B19 and a cocktail of lytic bacteriophages eradicated the C. perfringens KCTC $3269^T$, completely. Consequently, the isolated bacteriocin and bacteriophages represent candidates for effective biocontrol of C. perfringens, and bacteriocin-producing S. hyointestinalis B19 is a potential probiotic candidate for use in domestic animals.

Plasmid-associated Bacteriocin Production by Leuconostoc sp. LAB145-3A Isolated from Kimchi

  • Choi, Yeon-Ok;Ahn, Cheol
    • Journal of Microbiology and Biotechnology
    • /
    • v.7 no.6
    • /
    • pp.409-416
    • /
    • 1997
  • Leuconostoc sp. LAB145-3A isolated from kimchi produced a bacteriocin which was active against food pathogens, such as Listeria monocytogenes, Enterococcus faecalis, and E. faecium. Bacteriocin production occurred during the early exponential phase of growth and was stable upto the late stationary phase of growth. Optimum conditions for bacteriocin production were $37^{\circ}C$ with an initial pH of 7.0. The bacteriocin of LAB145-3A was sensitive to proteases, but stable for solvents, pH change and heat treatment. It was stable even at autoclaving temperature for 15 min. The bacteriocin exhibited a bactericidal mode of action against Lactobacillus curvatus LAB170-12. The bacteriocin produced by Leuconostoc sp. LAB145-3A was purified by CM-cellulose cation exchange column chromatography and Sephadex G-50 gel filtration. The purification resulted in an approximate 10,000-fold increase in the specific activity. Approximately 4% of the initial activity was recovered. Purified bacteriocin exhibited a single band on the SDS-PAGE with an apparent molecular weight of 4,400 daltons. This bacteriocin was named leucocin K. Leuconostoc sp. LAB145-3A had two residential plasmids with molecular sizes of 23 kb and 48 kb. A comparison of plasmid profiles between LAB145-3A and its mutants revealed that the 23 kb plasmid (pCA23) was responsible for bacteriocin production and immunity to the bacteriocin in Leuconostoc sp. LAB145-3A.

  • PDF

Characterization of a Bacteriocin Produced by Enterococcus sp. T7 Isolated from Humans

  • Moon, Hi-Seong;Jeong, Jong-Jin;Ji, Geun-Eog;Kim, Jong-Sang;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
    • /
    • v.10 no.4
    • /
    • pp.507-513
    • /
    • 2000
  • A bacteriocin-producing organism, Enterococcus sp. T7, was isolated from human fecal samples. Bacteriocin T7, named tentatively as the bacteriocin, was produced by Enterococcus sp. T7 and it inhibited some strains of Lactobacillus. Staphylococcus, Enterococcus, and Streptococcus, but not all the lactococci and gram-negative bacteria tested. Bacteriocin T7 inhibited the growth of Listeria monocytogenes Scott A, but the degree of inhibition was less than those for other sensitive gram-positive vacteria. Bacteriocin T7 in MRS broth started to produce at the middle of the exponential growth phase and the inhibitory activity reached its maximum level during the stationary growth phase. Bacteriocin T7 was stable against heat treatments, pH variations (pH 2-10), and exposure to organic solvents. The molecular weight of bacteriocin T7 was estimated to be 6.500 Da by SDS-PAGe. All these facts, including physico-chemical stabilities, small molecular size, and inhibition of Kisteria monocytogenes, indicate that bacteriocin T7 is likely to be a member of the class IIa bacteriocins.

  • PDF

Molecular Cloning of Bacteriocin Gene and Biological Control of Plant Pathogen (Bacteriocin 생산 유전자의 Cloning 및 식물병원균에 대한 생물학적 억제)

  • 김교창;육창수;도대홍
    • Microbiology and Biotechnology Letters
    • /
    • v.18 no.1
    • /
    • pp.98-102
    • /
    • 1990
  • A strain of Erwinia spp. was selected from the soil for the production of bacteriocin to the root rot plant pathogen. Bacteriocin producing gene was not located on plasmid but on chromosome. Genomic library of Erwinia spp. were made by using pLAFR 3 as a vector system for cloning of the gene. It was been cloned and expressed in E. coli DH 5 . Bacteriocin producing colony was composed of pLAFR 3 vector and 3.0 kb EcoRI fragment of Erwinia spp. ehromosomal DNA. The inserted fragment (3.0 kb) was possessed a EcoRI and BarnHI restriction sites.

  • PDF

Simple and Rapid Extraction of a Bacteriocin Produced by Streptococcus parauberis Z49 from Fermented Cultures (발효배양액에서 Streptococcus parauberis Z49균주가 생산하는 Bacteriocin의 간편한 추출)

  • Park, Hong-Je;Khang, Yong-Ho
    • Korean Journal of Microbiology
    • /
    • v.46 no.3
    • /
    • pp.291-295
    • /
    • 2010
  • A novel bacteriocin produced by Streptococcus parauberis Z49 strain was characterized and efficiently extracted from fermented cultures by use of aqueous two-phase systems. The nisin-like bacteriocin, which was active even after a heat treatment at $121^{\circ}C$ for 15 min and in the broad pH range from 2 to 12, showed inhibition of bacterial growth of Micrococcus luteus, Lactobacillus spp., Lactobacillus fermentum, Enterococcus faecium, Listereia monocytogenes, and Pseudomonas fluorescens. Optimal conditions of PEG 600/$Na_2SO_4$ aqueous two-phase systems for the simple and rapid extraction of a novel bacteriocin were determined to be PEG 600 15%, $Na_2SO_4$ 30%, and NaCl 8%, where the bacteriocin was concentrated in PEG layer.

Bacteriocins in Purple Nonsulfur Bacteria (홍색 비유황 광합성세균에서의 Bacteriocins에 관한 연구)

  • Lee, Sang Seob;Oh, Tae Jeong;Lee, Hyun Soon
    • Korean Journal of Microbiology
    • /
    • v.30 no.4
    • /
    • pp.265-268
    • /
    • 1992
  • In this study, we want to detect bacteriocin production in purple nonsulfur bacteria. As a results, it was showed that bacteriocin produced between some strains of Rhodobacter capsulatus, Rhodobacter sphaeroides and Rhodocyclus gelatinosus. In particular, it was appeared that cell membrane-bound bacteriocin was also produced by Rhodobacter capsulatus ATCC 17016.

  • PDF

Purification and Characterization of Bacteriocin Produced by Enterococcus sp. (Enterococcus sp.가 생산하는 Bacteriocin의 정제 및 특성에 관한 연구)

  • 정건섭;양은석;이국진;고현정;정병문
    • Microbiology and Biotechnology Letters
    • /
    • v.26 no.6
    • /
    • pp.523-528
    • /
    • 1998
  • We isolated microorganism secreting antimicrobial substance from tomato and identified as Enterococcus faecium. This substance was completely inactivated by pretense treatment and retained activity after catalase treatment. This result indicated that the antimicrobial activity of this substance was due to proteinaceous substance known as bacteriocin. The bacteriocin inhibited growth of Gram positive bacteria, such as Listeria monocytogenes, Leuconostoc mesenteroides, Lactobacillus plantarum, Streptococcus agalactiae, Streptococcus pyrogenes, and Gram negative bacteria, such as Pseudomonas aeruginosa. Purification of the bacteriocin was achieved by ethanol precipitation, ion exchange chromatography on CM Sepharose CL-6B, and gel filtration on Sephacryl S-100 HR. After these purification steps, the specific activity of the bacteriocin was increased 35.8 fold compared with culture broth. Purified bacteriocin was shown single band on SDS-PAGE and molecular weight was estimated 51 kDa. The residual activity of this bacteriocin was 3.3% at 10$0^{\circ}C$ for 60 min, and this bacteriocin was stable at pH 2~7.

  • PDF