• Title, Summary, Keyword: astragalin

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Synergic Effect of Quercetin and Astragalin from Mulberry Leaves on Ani-inflammation (상엽 유래 퀘세틴과 아스트라갈린의 항염증에 대한 상승효과)

  • Mok, Ji-Ye;Jeong, Seung-Il;Kim, Jang-Ho;Jang, Seon-Il
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.5
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    • pp.830-836
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    • 2011
  • The leaf of mulberry (Morus alba L) has long been used in Oriental medicine for the prevention or treatment of several diseases. However, little is known about the inhibitory effects of a single compound isolated from the mulberry leaves on inflammatory response. We are isolate a single compound of quercetin (3,3',4',5,7-pentahydroxyflavone) and astragalin (kaempferol-3-O-glucopyranoside) from the mulberry leaves, and then investigate the anti-inflammatory effects of quercetin, astragalin or quercetin plus astragalin in lipopolysaccharide (LPS)-stimulated murine peritoneal macrophages. Each compound suppressed the production of inflammatory mediators (NO, $PGE_2$ and IL-6) in LPS-stimulated murine peritoneal macrophages in a dose-dependent manner. Especially, the cotreatment of quercetin (2.5 ${\mu}M$) and astragalin (2.5 ${\mu}M$) markedly suppressed the production and the expression of inflammatory mediators. These suppressive effects were synergistically increased by their combination. These results suggest that the combination of quercetin and astragalin from the mulberry leaves may be useful for therapeutic drugs against inflammatory immune diseases, probably by suppressing the production of inflammatory mediators.

Isolation of Flavonoids from the Leaves of Aralia continentalis (땃두릅으로부터 Flavonoid 성분의 분리)

  • Kim, Ju-Sun;Kang, Sam-Sik;Lee, Myung-Whan;Kim, Ok-Kyung
    • Korean Journal of Pharmacognosy
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    • v.26 no.3
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    • pp.239-243
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    • 1995
  • Six flavonoids were isolated from the leaves of Aralia continentalis. Their structures were characterized as kaempferol, quercetin, 6'-O-acetyl astragalin, astragalin, trifolin and hyperoside by chemical and spectroscopic evidences. This is the first isolation from this plant.

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Chemical Constituents of Domestic Quercus spp. Leaves (국내산 참나무속 수종 잎의 추출성분)

  • Kim, Jin-Kyu;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.6
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    • pp.61-71
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    • 2006
  • This study was carried out to investigate chemotaxonomical correlation an d the chemical constituents of domestic Quercus sp. leaves. The leaves of Q. mongolica, Q. aliena, Q. serrata, Q. acutissima, Q. dentata and Q. variabilis were collected in the experimental forest of Kangwon National University. The combined extracts were successively fractionated with n -hexane, methylene chloride and ethyl acetate using a separation funnel. A portion of the ethyl acetate and $H_2O$ soluble materials of each species were chromatographed on a Sephadex LH-20 column using various aqueous MeOH and EtOH-hexane as washing solvents. Spectrometric analysis such as NMR and MS, including TLC, were performed to characterize the structures of the isolated compounds. Gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, kaempferol, astragalin, astragalin-6"-O-gallate, isoquercitrin, isoquercitrin-6"-O-gallate and myricetin were isolated from Q. mongolic a leaves. Gallic acid, kaempferol and quercetin were characterized from Q. acutissima leaves. Gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, (-)-epigallocatechin, kaempferol, quercetin, guajaverin and tamarixin were identified from Q. dentata leaves. Gallic acid, (+)-catechin, (-)-epicatechin, kaempferol, quercitrin, isoquercitrin and myricetin were purified from Q. serrata leaves. Gallic acid, (+)-catechin, astragalin, astragalin-6"-O-gallate and isoquercitrin were isolated from Q. variabilis leaves. Gallic acid was isolated from all the leaves and could be a taxonomic index on Quercus spp..

Isolation and Quantitative Analysis of Flavonoids with Peroxynitritescavenging Effect from the Young Leaves of Heracleum moellendorffii (어수리 어린 잎으로부터 Peroxynitrite 소거활성을 나타내는 플라보노이드 성분의 분리 및 함량분석)

  • Park, Hee-Juhn;Nugroho, Agung;Jung, Bo-Ram;Won, Yu-Hwa;Jung, Youn-Ju;Kim, Won-Bae;Choi, Jae-Sue
    • Korean Journal of Plant Resources
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    • v.23 no.5
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    • pp.393-398
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    • 2010
  • Naturally occurring substances which strongly scavenge peroxynitrite(ONOO-) are evaluated to be beneficial for cardiovascular, diabetic, hypercholesterolemic and obese disease. To search for bioactive substances with peroxynitritescavenging activity from the young leaves of Heracleum moellendorffii(Umbelliferae) used for a mountainous vegetable, the experiment on peroxynitrite-scavenging assay, phytochemical isolation and HPLC analysis were undertaken. Activity-guided fractionation led to the separation of astragalin and hyperoside which were identified by physicochemical and spectroscopic data. The $IC_{50}$ values of astragalin and hyperoside were shown as $5.80{\pm}0.62$ and $0.560{\pm}0.26\;{\mu}M$, respectively, in the assay. The HPLC analysis led to the quantitative determination of astragalin and hyperoside by $30.0{\pm}0.01$ and $17.0{\pm}0.02\;mg/g$ dried weight, respectively.

Flavonoids from the Aerial Parts of Lonicera japonica

  • Son, Kun-Ho;Park, Jung-Ok;Chung, Kyu-Charn;Chang, Hyeun-Wook;Kim, Hyun-Pyo;Kim, Ju-Sun;Kang, Sam-Sik
    • Archives of Pharmacal Research
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    • v.15 no.4
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    • pp.365-370
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    • 1992
  • Seven flavonoids were isolated from the aerial parts of Lonicera japonica. Their structures were characterized as hydnocarpin 1, quercetin 2, ochnaflavone 3, ochnaflavone 4'-O-methylether 4, astragalin 5, isoquercitrin 6, and rhoifolin 7 by chemical and spectroscopic evidences.

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Flavonoids from the Leaves of Polygalga japonica

  • Do, Jae-Chul;Yu, Young-Jun;Jung, Keun-Young;Son, Kun-Ho
    • Korean Journal of Pharmacognosy
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    • v.23 no.1
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    • pp.9-13
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    • 1992
  • From the leaves of Polygala japonica, kaempferol (1), astragalin (2), kaempferol $3-O-(6'-O-acetyl)-{\beta}-D-glucopyranoside$ (3) and kaempferol $3,7-di-O-{\beta}-D-glucopyranoside$ (4), have been isolated and characterized by chemical and spectral means.

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Phenolic Compounds of the Leaves of Eucommia ulmoides

  • Han, Suk-Young;Park, Jong-Cheol;Park, Hee-Juhn;Lee, Ji-Hyon;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.14 no.2
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    • pp.114-117
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    • 1991
  • From the leaves of Eucommia ulmoides, quercetin, astragalin, isoquercitrin, quercetin 3-O-$\beta$-D-sambubioside and chlorogenic acid were isolated and characterized by spectral data.

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Change of Medicinal Components by Different Species, Plant Parts and Growth Stage of Paeonia spp. (작약의 종(種), 부위 및 생육시기에 따른 성분 함량의 변화)

  • Kim Se-Jong;Park Jun-Hong;Kim Kil-Ung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.3
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    • pp.215-219
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    • 2006
  • This study was conducted to find to change of component by different species, plant parts and growth stage of Paeonia lactiflora Pallas Among the species of peony, the contents of compounds was higher in cultivated peony (P. lactiflora P.) as compared with wild peony (P. japonica M., P. obobata M., P. anomala L.). Amount of methyl gallate was highest in 0.45% at Euisung jakyak. Amount of compounds in peony was the highest in 1.0% at paeoniflorin, followed by methyl gallate, astragalin and kaempferol in order. Contents of compounds with different growth stage were observed highest in April, and showed decreased trend in the later growth stage. Methyl gallate was present in 1.79% at petal, 0.56% at leaf and 0.01% at root, astragalin present at 0.27% at petal, 0.20% at leaf and 0.03% at root, and paeoniflorin present at 0.43% at petal, 1.09% at leaf and 2.52% at root.