• Title/Summary/Keyword: ascorbic acid

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Monitoring Decreases in the Patulin Level of Apple Juice using Response Surface Methodology (반응표면 분석에 의한 사과 주스의 Patulin 감소 조건 모니터링)

  • Baek, Chang-Ho;Park, Nan-Young;Jeong, Yong-Jin
    • Korean Journal of Food Preservation
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    • v.17 no.1
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    • pp.84-90
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    • 2010
  • We investigated methods to minimize patulin content during processing of apple juice in Korea. Ascorbic acid concentration (100, 200, 300, and 400 ppm) and activated carbon concentration (50, 100, 150, and 200 ppm) were the parameters assessed by response surface methodology. Residual patulin was lowest in the presence of 237.77 ppm ascorbic acid and 106.58 ppm activated carbon, and increased as these levels increased. Sugar content was affected more byascorbic acid concentration than by activated carbon level, and the total phenol content was revealed to be the saddle point. In addition, the expected total flavonoid content was maximized with increased levels of ascorbic acid and activated carbon. The data showed that 246-274 ppm (v/v) ascorbic acid and 93-122 ppm (v/v) activated carbon were optimal for juice extraction, and the $R^2$ quality factor for juice processing was significant and within the range of 1-10% for all tested parameters.

Effects of Combined Treatment with High $CO_2$ Concentration and Ascorbic acid on Browning of Fresh-cut 'Fuji' Apples (고농도 $CO_2$와 Ascorbic acid 처리가 신선절단 사과의 갈변에 미치는 영향)

  • Kim, Sun-Young;Cho, Jung-Seok;Jeong, Moon-Cheol;Moon, Kwang-Deog
    • Korean Journal of Food Preservation
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    • v.18 no.4
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    • pp.475-480
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    • 2011
  • This papers were studies the effects of high $CO_2$ concentration pretreatment and ascorbic acid on the browning of fresh-cut apples. The prepared samples were dipped in 1% (w/v) ascorbic acid solution (AA). Fresh-cut apples (3 pieces) were packed in polypropylene bags (0.03 mm, $20{\times}15$ cm), and stored at $10^{\circ}C$ for 16 days. AA-treated samples showed higher L value than those of non-treated and control (dipped in water without $CO_2$ pretreatment), while non-treated samples showed higher hardness than AA-treated sample. Total soluble solids, pH, titratable acidity were not affected by high $CO_2$ pretreatment and AA treatment. These result shows high $CO_2$ concentration pretreatment with dipping in 1% ascorbic acid solution (AA) has an effects on controlling enzymatic browning during the storage of fresh-cut 'Fuji' apples.

A Study of Urinary Ascorbic Acid as a Noninvasive Biomarker for Cadmium-Intoxication in Rats (카드뮴 중독 흰쥐에 대한 Noninvasive Biomarker로서의 요중 Ascorbic Acid에 관한 연구)

  • 안승주;김용호;류재두;이용우
    • Biomedical Science Letters
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    • v.3 no.2
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    • pp.151-160
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    • 1997
  • This study was planned to evaluate the urinary ascorbic acid as a new biological marker for the intoxication of cadmium, which could possibly be driven by its increased utilization and environmental pollution. In order to meet this goal, we have peformed measurement of urinary ascorbic acid concentration, histopathological examination of the kidney, and biochemical test for the liver function using cadmium-intoxicated rats by oral administration. The average concentrations of urinary ascorbic acid in the $CdCl_2$-treated rats were 214.0 mg/dl for 100 ppm group and 254.3 mg/dl for 200 ppm group during experimental period of 50 days. These levels are 24 and 28 times higher than one in the control group (9.0 mg/dl), respectively. Ultrastructural study showed the eosinophilic hyaline cast and focal effacement, fusion in the renal tubules, as well as loss of foot processes on the glomerular epithelial cells. These results suggested that cadmium may be responsible for renal glomerular injury. The blood levels of AST, ALT and LDH in the treated groups (199 IU/I, 88 IU/I, 1190 U/I for the 100 ppm group and 270 IU/I, 226 IU/I, 760 U/I for the 200 ppm group) were higher than ones in the control group(143 IU/I, 50 IU/I, 334 U/I). These results indicated the cadmium induced the damage of liver function. In conclusion, the administration of cadmium showed a remarkable increase of urinary ascorbic acid with renal and hepatic damage. Therefore, it is expected that measurement of urinary ascorbic acid would be an powerful method as a noninvasive biomarker for cadmium intoxication.

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Synthesis of L-Ascorbic Acid Derivative Including 3-Aminopropane Phosphoric Acid as a Novel Whitening Agent

  • Kang, Hak-Hee;Oh, Seong-Geun
    • Bulletin of the Korean Chemical Society
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    • v.24 no.8
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    • pp.1169-1171
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    • 2003
  • A stable derivative of L-ascorbic acid, 2-O-[(3-aminopropyl)phosphinooxy]-L-ascorbic acid (LAAP), was synthesized in moderate yield and its chemical stability and effects on melanin synthesis were investigated. LAscorbic acid was decomposed completely within about 1 hour, while 93% of LAAP remained even after 10 days. Treatment of L-ascorbic acid and LAAP decreased melanin content in normal human melanocytes to 33.8% and 49.1% of control at 2 mM, respectively. Considering chemical instability of L-ascorbic acid, LAAP is a much better whitening agent.

Action of Ascorbic Acid and Indoleacetic Acid on the Oxidation of Succinate and Coupled Phosphorylation in Chlorella Mitochondria (Chlorella Mitochondria의 Oxidative Phosphorylation에 대한 Ascorbic Acid 및 IAA의 작용성에 관하여)

  • Lee, Yung-Nok;Chin, Pyung
    • Korean Journal of Microbiology
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    • v.2 no.1
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    • pp.12-16
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    • 1964
  • Lee, Yung Nok and Chin, Pyung (Dept. of Biology, Korea University, Seoul, Korea) : Action of ascorbic acid indoleacetic acid on the oxidation of succinate and coupled phosphorylation in Chlorella mitochondria. Kor. Jour. Microbiol., Vol.2, No.1, p12-16 (1964) Mitochondria were isolated from Chlorella ellipsoidea and the action of ascorbic acid and indoleacetic acid on the succinate oxidation and coupled phosphorylation in mitochondria suspension were examined. Oxidation of succinate used as substrate, and phosphorylation coupled to oxidation were strikingly enhanced by the addition of ascorbic acid, while in case of indoleacetic acid it were a little. In a view of phosphorylative efficiency, P/O ratio resulting from the addition of ascorbic acid was decreased and it may be considered as the result of a partial oxidation of ascorbate in mitochondria.

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Degradation of Ascorbic acid in Limited Dissolved Oxygen Environment (제한된 산소 존재중의 Ascorbin 산의 분해에 대하여)

  • Rhee, Sook-Hee
    • Journal of Nutrition and Health
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    • v.9 no.4
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    • pp.54-58
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    • 1976
  • Deterioration of ascorbic acid content is the important factor in the food quality. The degradation of ascorbic acid undergoes as a first-order of reaction in the presence of excess oxygen content. However, under the limited oxygen content, ascorbic acid decomposes as a psudo-first order of reaction. The ascorbic acid, in this study, under the limited dissolved oxygen content in the presesce of iron(III) and copper(II) decomposed as first-order reaction with a little influence in rate constant and the light was an accelerating factor on the ascorbic acid degradation.

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Changes of Ascorbic Acid Contents Induced from Gamma Irradiation, Heating and Microwave Treatments (방사선 조사, 가열 및 마이크로웨이브처리에 따른 Ascorbic Acid의 함량변화)

  • 변명우;이인숙;이경행;육홍선;강근옥
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.4
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    • pp.954-957
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    • 1999
  • The changes in L ascorbic acid content by processing treatments; gamma irradiation, heating and microwave were investigated using high performance liquid chromatography. The content of L ascorbic acid in standard solutions and citrus fruits decreased from 27.4 to 44.9% and from 6.9 to 21.9%, re spectively, by gamma irradiation doses in the range of 1 to 10 kGy. By heating treatments, L ascorbic acids in standard solutions and citrus fruits were destroyed 22.5 to 36.8% and 4.5 to 18.1%, respectively. By microwave treatment, L ascorbic acid content also decreased from 23.1 to 47.4% and from 6.5 to 22.6%, respectively.

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Viability of Probiotic Bacteria in Yogurt Supplemented with Enzyme-Bioconverted Ginseng, Ascorbic Acid, and Yeast Extract (효소처리인삼, 아스코르브산, 효모추출물이 첨가된 요구르트에서 프로바이오틱 세균의 활성)

  • Choi, Suk-Ho;Lim, Young-Soon
    • Journal of Dairy Science and Biotechnology
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    • v.37 no.1
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    • pp.57-68
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    • 2019
  • The effects of yogurt supplementation with enzyme-bioconverted ginseng (EBG), ascorbic acid, and yeast extract on the bacterial counts of Streptococcus thermophilus, Lactobacillus acidophilus LA-5, and Bifidobacterium BB-12 were investigated to develop healthy yogurts with high probiotic counts during storage. In addition, the colors and viscosities of the yogurts were determined. EBG, ascorbic acid, and yeast extract did not affect S. thermophilus counts. EBG and ascorbic acid enhanced the viabilities of L. acidophilus LA-5 and Bifidobacterium BB-12 during storage. Yeast extract improved growth of L. acidophilus LA-5 and Bifidobacterium BB-12 during fermentation. EBG turned the yogurt into brown color. We conclude that supplementation of yogurt with EBG, ascorbic acid, and yeast extract may enhance its health-promoting functions by increasing the viability of probiotics, which can thus promote consumption of the yogurt.

Tissue-Based Amperometric Biosensor for Determination of L-Ascorbic Acid in Organic Media (유기 용매 속에서 L-Ascorbic Acid의 정량을 위한 바이오센서)

  • Kwon, Hyo-Shik;Lee, Cheal-Gyu
    • Analytical Science and Technology
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    • v.7 no.2
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    • pp.149-153
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    • 1994
  • An amperometric sensor for L-ascorbic acid(AA) in methanol media has been made by immobilizing squash-tissues on a graphite rod disk. A detection limit of the electrode was $2{\times}10^{-6}M$ L-ascorbic acid. In comparison with an isolated enzyme based ascorbate oxidase(AO) electrode, the plant-tissue electrode offered high biocatalytic stability and activity and extremely low cost. The electrode has a useful lifetime of 1 week.

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Ascorbic Acid와 Pyrophosphate로부터 Ascorbic Acid-2-Phosphate의 효소적 생산

  • 최현일;이상협;방원기
    • Microbiology and Biotechnology Letters
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    • v.24 no.5
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    • pp.613-618
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    • 1996
  • Microorganisms capable of producing ascorbic acid-2-phosphate (AsA2P) from ascorbic acid (AsA) and pyrophosphate (PPi) were screened from the culture collection of this laboratory. Among them, Cellulomonas sp. AP-7 showed the highest productivity of AsA2P. The optimal conditions for the production of AsA2P from AsA and PPi with cell-free extract as an enzyme source were investigated. The reaction mixture for the maximal production of AsA2P consisted of 21 g protein of cell-free extract per liter as the enzyme source, 250 mM AsA, 200 mM sodium pyrophosphate, 150 mM sodium acetate buffer (pH 4.5). By using this reaction mixture, 31.9 mM of AsA2P, which corresponded to a 12.76% yield based on AsA, was produced after incubation of 48 hr at 33$\circ$C.

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