• Title/Summary/Keyword: ascorbic acid

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Celluomonas sp. AP-7이 생산하는 Ascorbic Acid Phosphorylating Enzyme의 정제 및 특성

  • 이상협;최현일;방원기
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.271-276
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    • 1997
  • An ascorbic acid phosphorylating enzyme, which catalyzes the formation of ascorbic acid-2-phosphate from ascorbic acid and pyrophosphate, was purified 32.7-folds to homogeneity from a cell-free extract of Cellulomonas sp. AP-7. The combination of DEAE- Sephacel ion exchange chromatography and Sephacryl S-200 get filtration was used for their purification. The molecular weight of the native protein was estimated to be 96.lkDa on high performance gel filtration chromatography. The SDS-PAGE analysis indicated that the protein consisted of four identical subunits of 24.6 kDa. The purified enzyme showed the optimal tempeature of 40$\circ$C and optimal pH of 4.5. The Km for ascorbic acid and pyrophosphate were 119 mM and 11.9 mM, respectively. The addition of 5,5'-dithiobis-(2-nitrobenzoic acid) into the reaction mixture resulted in the reduction of the enzyme activity at 51%. The enzyme also had a phosphatase activity at weakly acidic pH and the Km for ascorbic acid-2-phosphate in phosphatase activity was 7.9 mM.

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Studies on the Synthesis of L-Ascorbic acid-3-Aminopropane Phosphoric Acid Diester and its Applications (L-Ascorbic acid-3-aminopropane phosphoric acid diester의 합성과 응용에 관한 연구)

  • 이옥섭;이기화
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.23 no.2
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    • pp.97-117
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    • 1997
  • L-Ascorbic acid 및 그 유도체는 항산화 작용과 미백작용 및 섬유아세포의 생장 촉진과 콜라젠 생합성의 증가시키는 효과가 있으므로 화장품에서 오래 전부터 사용되어 왔다. 본 연구에서는 생체에 대한 안전성과 안정성이 우수한 L-Ascorbic acid 유도체를 개발하기 위하여 인지질과 유사하게 L-Ascorbic acid 에 3-Aminopropane phosphoric acid를 결합하여 L-Ascorbic acid-3-aminopropane phosphoric acid diester를 합성하였다. ASA-APPA 는 2-Chlorotetrahydro-2H-1, 3, 2-oxazaphosphorine P-oxide 와 5, 6-isopropylidene L-Ascorbic acid 를 반응 시킨 후 산 사수분해 반응을 통하여 얻을 수 있었다. ASA-APPA는 수용액에서 안정성이 우수하였으며, 독성실험에서 무독성 물질이며, 인체 첩포 실험에서도 무자극 물질임을 확인하였다. 그리고, ASA-APPA 는 L-Ascorbic acid 와 3-APPA 의 혼합물과 거의 유사한 섬유아세포의 증식효과를 나타내었으며, melanoma 에 대한 멜라닌 생성 억제 실험에서 L-Ascorbic acid phosphate magnesium salt 와 유사한 효과를 보였다. 따라서 ASA-APPA 는 멜라닌 생성 억제 효과와 섬유아세포의 증식 효과를 갖는 새로운 화장품 원료로서 사용이 가능할 것으로 생각된다.

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Action of Ascorbic acid on Sodium-Potassium activated ATPase in Red Cell Membrane (적혈구막의 NaK ATPase의 활성도에 대한 ascorbic acid의 작용)

  • Koh, Il-Sup
    • The Korean Journal of Physiology
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    • v.12 no.1_2
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    • pp.15-23
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    • 1978
  • The action of ascorbic acid on the sodium Plus potassium activated ATPase activity in the rabbit red cell membrane has been investigated and the experiments were also designed to determine the mechanism of action if ascorbic acid on the ATPase activity The following results were observed. 1. The activity of the NaK ATPase from red cell membrane is stimulated by ascorbic acid and the concentration of ascorbic acid for maximal activity is about 8 mM. 2. The activating effect of ascorbic acid on the ATPase activaty, with a given concentration of sodium in the medium, is increased by raisins the potassium concentration but activity ratio is decreased. 3. The activating effect of ascorbic acid on the ATPase activity, with a given concentration of potassium in the medium, is increased by raising the sodium concentration but activity ratio is decreased. 4. The action of ascorbic acid on the ATPase activity is stimulated by calcium ions and activity ratio is increased by raising the calcium concentration. 5. The activating effect of ascorbic acid on the ATPase activity was not related to the sulfhydryl group of cysteine or the hydroxyl group of threonine. 6. The activating effect of ascorbic acid on the ATPase activity is due to amino group and carboxyl group of the enzyme of NaK ATPase.

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The study on correlation between the levels of ascorbic acid in diet and serum (우리나라 여대생의 식이와 혈청이 ascorbic acid 수준의 상관관계 평가 - 섭취량 계산방법에 따른 비교 연구 -)

  • 계승희
    • Journal of the Korean Home Economics Association
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    • v.32 no.1
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    • pp.181-188
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    • 1994
  • The purpose of this study is to evaluate nutritional status of ascorbic acid in 96 Korean female college students. Dietary intake level was estimated with three day diet records. Serum ascorbic acid were analyzed with fasting blood. Nutrient intakes was calculated using three different food composition databases ; analysis data of cooked food items ; analysis data of raw foods ; published food composition table. Ascorbic acid intake levels calculated with different food composition databases differed by 15% Using analysis data of cooked foods resulted in the lowest intake level 49.26 mg per day. Main sources of ascorbic acid investigated were Cheju mandarin orange juice and hard persimmon orange juice and Kimchi. Mean value of serum ascorbic acid level was 1.04 mg/dl and 4.2% of subjects were in marginal risk or deficient range. It was found to significantly correlate asscorbic acid intake with serum ascorbic acid content. Correlation coefficient with results calculated using analyzed data was higher than that using published data. Serum levels and mean daily intake levels of ascorbic acid in the subjects were significantly correlated. From these results it is concluded that ascorbic acid content of cooked foods should be included in the Food Composition Tables in Korea. Korean female college students were in lower risk of ascorbic acid deficiency.

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Effect of Guibitang and Ascorbic Acid on the Regional Brain Catecholamines contents of Heat or Forced Swimming stressed Guinea Pig (귀비탕(歸脾湯)과 Ascorbic Acid가 열(熱) 및 유영(遊泳) Stress Guinea Pig의 뇌(腦) Catecholamine 함량(含量)에 미치는 영향(影響))

  • Ryu Jae-Gyu;Whang Wei-Wan
    • Journal of Oriental Neuropsychiatry
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    • v.6 no.1
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    • pp.41-49
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    • 1995
  • This study aimed to compare the anti-stress effect of Guibitang with Ascorbic Acid on the guinea pigs in heat or forced swimming stress. the experimental animals were heated in hot room$(34{\pm}2^{\circ}C)$ for 2 hours in a day during 7 days. Forced swimming stress was loaded by forcing guinea pigs to swim in a oval tub for 2 minutes in a day during 7days. And administered 1.0mg/100g of Ascorbic Acid or 86.0mg/100g of Guibitang extract formonce befor stress. 1. In brain, the contents of norepinephrine in group of the heat of forced swimming stress, in case administered Ascorbic Acid and in case administered Guibitang extract with Ascorbic Acid significantly decreases as compared with these in case administered non Ascoric Acid. 2. In brain, the contents of epinephrine in group of the forced swimming stress, in case administered Guibitang extract with Ascorbic Acid significantly decreases as compared with these in case administered non Ascorbic Acid. 3. In brain, the contents of dopamine in group of the heating stress, in case administered Guibitang extract with Ascorbic Acid significantly decreases as compared with these in case administered non Ascorbic Acid, and these in group of the forced swimming stress in case administered Ascorbic Acid and in case administered Guibitang extract without Ascorbic Acid and in case administered Guibitang extract with Ascorbic Acid significantly decreases as compared with these in case administered non Ascorbic Acid.

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형질전환된 Nicotiana tabacum 배양에 있어서 glutathione과 ascorbic acid가 세포생장과 생존율에 미치는 영향

  • Kim, Yong-Hun;Lee, Sang-Yun;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
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    • pp.293-297
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    • 2003
  • Glutathione and ascorbic acid have been shown to fulfill many essential functions in animal and plant growth, development, defence and protection against oxidative damage. Effects of glutathione and ascorbic acid were examined in transgenic N. tabacum cells producing hGM-CSF to determine the effects of the vitamins on growth and cell viability. In lag phase, cell viability was preserved by glutathione and ascorbic acid. Therefore, recombinant protein productivity was increased. The purpose of present study is to investigate the role of antioxidants in cold stress-induced apoptosis in plant suspension cells. Cold stress lowered cell viability and increased total genomic DNA fragmentation. Supplementing the cell cultures with glutathione and ascorbic acid inhibited cold stress-induced decrease in cell viability and increase in total genomic DNA fragmentation.

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The effects of ascorbic acid on the morphology and cell proliferation of three-dimensional stem cell spheroids (아스코르브산의 3차원 줄기세포 배양체에 대한 증식 및 분화 효과 분석)

  • Lee, Hyunjin;Yeo, Seong-Il;Park, Jun-Beom
    • The Journal of the Korean dental association
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    • v.55 no.9
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    • pp.592-603
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    • 2017
  • Purpose: The effects of various concentrations of ascorbic acid on stem cell spheroids derived from intraoral areas are not known yet. Thus, the purpose of this study is to evaluate the effects of different concentrations of ascorbic acid on the morphology and cellular viability of stem cell spheroids derived from the gingival tissues. Materials and Methods: Stem cells were plated onto silicon elastomer-based concave microwells and grown in the presence of ascorbic acid at concentrations ranging from 0.003% to 0.3%. The morphology of the cells was viewed under an inverted microscope at day 1, 2, 3 and 5. Qualitative live/dead assay and quantitative cellular viability using Cell Counting Kit-8 were performed on day 2 and day 5. Results: Gingiva-derived stem cells formed spheroids irrespective of ascorbic acid concentration in silicon elastomer-based concave microwells. Increase in the diameter of spheroid were seen with higher concentrations of ascorbic acid. Higher cellular viability was seen in higher concentrations of ascorbic acid. Conclusion: Within the experimental setting, the application of ascorbic acid on stem-cell spheroids produced an increase in the size and higher viability with higher dosage. It can be suggested ascorbic acid be applied with stem cell spheroids for tissue engineering purposes.

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Study on the Absorption Rate of Ascorbic Acid with Span and Tween (Span과 Tween이 Ascorbic Acid의 흡수효율(吸收效率)에 미치는 영향(影響))

  • Kim, Chun-Kyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.4
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    • pp.43-46
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    • 1982
  • The purpose of this paper was to study the effect of surfactants on the absorption and the bioavailability of ascorbic acid in the small intestine of rats and rabbits absorption of ascorbic acid in small intestine of rats was increased with tween 80 and span 80.The blood level of ascorbic acid in rabbits was elevated by combined with low concentration of surfactants but high concentration of surfactants. The bioavailability of ascorbic acid was increased by simultaneous administration of surfactants.

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Functions and Metabolic Pathway of Ascorbic Acid in Plant (식물의 Ascorbic Acid의 기능과 대사령로)

  • Park Yang-Ho;Lee Ju-Young;Jang Byoung-Choon;Lee Ki-Sang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.457-461
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    • 2005
  • During the last few years, considerable progresses have been made in understanding of roles and biosynthesis of ascorbic acid (AsA) in plants. The concentrations of ascorbic acid is 2-4 mM in leaf cells, but much higher at the chloroplast. There are three forms of ascorbic acid in the plant mainly ascorbic acid (AsA), monode­hydroascorbic acid (MDHA) and dehydroascorbic acid (DHA). AsA in plant cell performs antioxidants by changing those three forms. And AsA promotes cell division and elongation There was new pathway of ascorbic acid metabolism. It is called pathway of Smirnoff-Wheeler. This report will provide understanding of AsA in plants, and also provide

Study on the Effect of Talc on the Degredation of Ascorbic Acid in its Diluted Preparation (분말성제제(粉末性製劑)의 안정성(安定性)에 관(關)한 연구(硏究) -제제중(製劑中)의 Ascorbic Acid의 분해(分解)에 미치는 Talc의 영향(影響)-)

  • Chung, Kee-Hwa;Lee, Min-Hwa;Woo, Chong-Hak;Kim, Shin-Kenn
    • Journal of Pharmaceutical Investigation
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    • v.2 no.1
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    • pp.52-57
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    • 1972
  • There are many reports on the stability of drugs in powders and various dosage forms. The author studied on the stability of ascorbic acid mixed with talcs. From the result of this experiment, it was considered that talcs markedly accelerated oxidative degredation of ascorbic acid in powders and the acceleration rate depends on the varieties of crude talcs. Iron salts seem to be the principal factor influencing the degradation of ascorbic acid Degradation rate of ascorbic acid mixed with purified talc is significantly slower than that with the crnde talcs. The more the concetration of ascorbic acid is diluted, the faster the degradation rate and degredation rate of Ascorbic acid is the function of tme.

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