• Title/Summary/Keyword: ascorbic acid

Search Result 1,995, Processing Time 0.1 seconds

Production of Ascorbic acid-2-phosphate from Ascorbic acid by Pseudomonas sp.. (Pseudomonas sp.에 의한 Ascorbic acid로부터 Ascorbic acid-2-phosphate의 생산)

  • 권기성;이상협;방원기
    • Microbiology and Biotechnology Letters
    • /
    • v.28 no.1
    • /
    • pp.33-38
    • /
    • 2000
  • In order to produce ascorbic acid-2-phosphate from ascorbic acid, bacteria capable of transforming ascorbic acid to ascorbic acid-2-phosphate were isolated from soils and the stock cultures in our laboratory. Among them, a newly isolated bacterium LSH-3 having the best ability of producing ascorbic acid-2-phosphate was selected and partially identified as Pseudomonas sp. The optimum conditions for the production of ascorbic acid-2-phosphate from ascorbic acid and using its resting cells as the source os enzyme were investigated. The results were summarized as follows: The optimum cultivation time and the cell weight for the production of ascorbic acid-2-phosphate was 14 hours and 100g/I(wet weight), respectively. And 0.1%(v/v) Trition X-100 was the most effective surfactant. The optimum concentrations of ascorbic acid and pyrophosphate were 400mM and 500mM, respectively, which led to produce 14.54g/I of ascorbic acid-2-phosphate. The most effective buffer was 50mM sodium acetate. The optimum pH and temperature were 4.5 and $40^{\circ}C$, respectively. Under the above conditions, 17.71 g/I of ascorbic acid-2-phosphate was produced from ascorbic acid after 32 hour-incubation, which corresponded to 17.5% of conversion rate based on ascorbic acid.

  • PDF

The Comparative Studies for the Anti-stress Effect of Guibitang and Ascorbic Acid on Heating and Swimming Stress (열(熱)Stress 및 유영(游泳)Stress에 대(對)한 귀비탕(歸脾湯)과 Ascorbic Acid의 항(抗)Stress효능(效能) 비교연구(比較硏究))

  • Lee Hwa-Sin;Whang Wei-Wan
    • Journal of Oriental Neuropsychiatry
    • /
    • v.6 no.1
    • /
    • pp.19-39
    • /
    • 1995
  • In order to compare the anti-stress effect of Guibitang with that of ascorbic acid, after these medicines were administered to guinea pigs induced by heating and swimming stress, the changes of the weight of body and organ, and content of plasma catecholamines, serum total cholesterol, free cholesterol, triglyceride, protein, glucose and cortisol were measured. The results were as follows : 1. The weight of the body was increased with statistical significance in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on both heating and swimming stress. 2. The weight of spleen decreased with statistical significance in the groups administered ascorbic acid, Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress, but in case of swimming stress, the weight of spleen decreased with statistical significance in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. The weight of adrenal decreased with statistical significance in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress alone. 3. Plasma norepinephrine content decreased with statistical significance not only in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid but in the group administered ascorbic acid with Guibitang as compared with in the group administered ascorbic acid on heating stress. In case of swimming stress, norepinephrine decreased with statistical significance in the groups administered ascorbic acid, Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. 4. Plasma dopamine content decreased with statistical significance only in the group administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on both heating and swimming stress. 5. Serum total cholesterol content decreased with statistical significance in the groups administered ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress, but in case of swimming stress, it decreased with statistiscal significance only in the group administered ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. 6. Serum triglyceride content decreased with statistical significance not only in the groups administered ascorbic acid, Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid but in the group administered ascorbic acid with Guibitang as compared with in the group adminstered ascorbic acid on heating stress. In case of swimming stress, triglyceride decrease with statistical significance only in the group administered ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. 7. Serum glucose content increased with statistical significance in the groups administered Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on both heating and swimming stress, particulaly in case of swimming stress, in the group administered ascorbic acid with Guibitang, it increased with statistical significance as compared with in the group administered ascorbic acid. 8. Serum cortisol content decreased with statistical significance only in the group administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress.

  • PDF

Characteristics of Ascorbic acid Oxidase in Cucumbers (오이의 Ascorbic Acid Oxidase에 관한 연구)

  • Kim, Jung-Won;Park, Eun-Soon;Yoon, Sun
    • Journal of Nutrition and Health
    • /
    • v.18 no.4
    • /
    • pp.312-317
    • /
    • 1985
  • This study was attempted to investigate the occurrence and the characteristics of ascorbic oxidase in cucumbers. Ascorbic acid oxidase was isolated from cucumbers and concentrated using ammonium sulfate precipitation. The results of this study are as follows ; 1) Ascorbic acid oxidase activity was detected in whole cucumber homogenate. 2) Highest amounts of ascorbic acid destroyed after 10 minutes' incubation of ascorbic acid oxidase with its substrate. 3) The optimum pH and temperature of this enzyme were found to be pH 6.5 and $40^{\circ}C$, respectively. 4) Ascorbic acid content in cucumber juice prepared using the cold water $(4^{\circ}C)$ was higher than that made with water at $30^{\circ}C$. 5) When orange juice ( pH 3.4 )was added, ascorbic acid destruction was completely ceased. (The ascorbic acid oxidase was inactivated at pH 3.9) Decreasing the temperature and pH are recommended to achieve maximum stability of ascorbic acid in preparing cucumber juice.

  • PDF

The Microencapsulated Ascorbic Acid Release in vitro and Its Effect on Iron Bioavailability

  • Lee, Jun-Beum;Ahn, Joung-Jwa;Lee, Jong-Hwi;Kwak, Hae-Soo
    • Archives of Pharmacal Research
    • /
    • v.26 no.10
    • /
    • pp.874-879
    • /
    • 2003
  • The present study was carried out to examine the stability of microencapsulated ascorbic acid in simulated-gastric and intestinal situation in vitro and the effect of microencapsulated ascorbic acid on iron bioavailability. Coating materials used were polyglycerol monostearate (PGMS) and medium-chain triacylglycerol (MCT), and core materials were L-ascorbic acid and ferric ammonium sulfate. When ascorbic acid was microencapsulated by MCT, the release of ascorbic acid was 6.3% at pH 5 and 1.32% at pH 2 in simulated-gastric fluids during 60 min. When ascorbic acid was microencapsulated by PGMS, the more ascorbic acid was released in the range of 9.5 to 16.0%. Comparatively, ascorbic acid release increased significantly as 94.7% and 83.8% coated by MCT and PGMS, respectively, for 60 min incubation in simulated-intestinal fluid. In the subsequent study, we tested whether ascorbic acid enhanced the iron bioavailability or not. In results, serum iron content and transferring saturation increased dramatically when subjects consumed milks containing both encapsulated iron and encapsulated ascorbic acid, compared with those when consumed uncapsulated iron or encapsulated iron without ascorbic acid. Therefore, the present data indicated that microencapsulated ascorbic acid with both PGMS and MCT were effective means for fortifying ascorbic acid into milk and for enhancing the iron bioavailability.

Effect of Liposome on the Stabilization of Ascorbic Acid (Ascorbic Acid 의 안정성에 대한 Liposome 의 효과)

  • Lee, Yu-Weon;Hwang, Yong-Il;Lee, Seung-Cheol
    • Korean Journal of Food Science and Technology
    • /
    • v.31 no.2
    • /
    • pp.280-284
    • /
    • 1999
  • To overcome unstability of ascorbic acid, liposome was used to encapsulate it. Ascorbic acid was encapsulated with 46.8% efficiency inside soybean phosphatidyl choline liposomes by the dehydration-rehydration method. Stability of encapsulated ascorbic acid in liposome was enhanced compared to that in free aqueous solution. For example, most of ascorbic acid in acetate buffer (pH 5.0) was oxidized after 7 days, however, that in liposome was remained as reduced form with 22.8% after 40 days at same conditions. These results mean that encapsulation of ascorbic acid in liposome could provide protection tool for improvement in shelf life.

  • PDF

The Constraint for Oxidation of Ascorbic Acid (Ascorbic Acid의 산화억제)

  • 이강연;한창규;조춘구
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.25 no.3
    • /
    • pp.67-86
    • /
    • 1999
  • Ascorbic acid which has various physiological benefits as the functional substance is easily oxidized and destroyed by the structural instability. Liposome encapsulated pure ascorbic acid was prepared for the sake of the constraint of oxidation. The influence of cholestrol or $\beta$-sitosterol on the stabilization of liposome was investigated. Butylated hydroxytoluene(BHT), tertiary butylhydroquinone(TBHQ), $\alpha$ -glycosyl rutin and natural concentrated tocopherol were used for constraint of oxidation of ascorbic acid. The presence of cholesterol or $\beta$-sitosterol decreased oxidation of ascorbic acid. That results were thought that cholesterol or $\beta$-sitosterol so increased rigidity of bilayer that the leakage of vitamin C decreased. As a result the oxidation and degradation of vitamin C were constrained. At 0.3w/w% cholesterol content the most stable liposome was formulated. The whole antioxidant that used at the research constrained oxidation of ascorbic acid. The antioxidation for ascorbic acid increased in order of tertiary butylhydroquinone, $\alpha$-glycosyl rutin, butylated hydroxytoluene and natural concentrated tocopherol. But u -glycosyl rutin is preferable to tertiary butylhydroquinone which was the most effective in antioxidation as the antioxidant of ascorbic acid which was utilized in cosmetics and pharmacy.

  • PDF

The Role of Ascorbic Acid on the Redox Status and the Concentration of Malondialdehyde in Streptozotocin-Induced Diabetic Rats

  • Choi, Hee-Jung;Je, Hyun-Dong;Jeong, Ji-Hoon;Min, Young-Sil;Choi, Tae-Sik;Park, Joon-Hong;Shin, Chang-Yell;Sohn, Uy-Dong
    • Archives of Pharmacal Research
    • /
    • v.26 no.3
    • /
    • pp.237-243
    • /
    • 2003
  • We investigated the role of ascorbic acid on the redox status in streptozotocin-induced diabetic rats. In the plasma of diabetic rats, the ratio of reduced/total ascorbic acid was significantly decreased as compared with normal control. Ascorbic acid supplementation increased the reduced and total ascorbic acid contents as compared with diabetic control. In the rutintreatment group, reduced and total contents of ascorbic acid were significantly decreased, however, the ratio of reduced/total contents of ascorbic acid had no difference as compared with diabetic rats. In the insulin-treatment group, this ratio is not significantly different as compared with diabetic control. However, in the insulin plus ascorbic acid treatment group, reduced form and the ratio of reduced/total ascorbic acid were significantly increased as compared with diabetic control. In addition, we measured the contents of malondialdehyde (MDA) in the plasma of diabetic rats. The contents of MDA was increased as compared with normal control, however, in insulin-treatment group, the contents of MDA was decreased as compared with diabetic rats. Ascorbic acid had no effects on the increases of MDA in diabetic rats. In conclusion, plasma ascorbic acid level and its reduced/total ratio reflects the status of the oxidative stress in the diabetic rats. Supplement of ascorbic acid did not correct the ratio of the reduced/total ascorbic acid. However, supplement of insulin and ascorbic acid corrected the ratio of reduced/total ascorbic acid.

Microencapsulated Ascorbic Acid for Milk Fortification

  • Lee, J.B.;Ahn, J.;Kwak, H.S.
    • Archives of Pharmacal Research
    • /
    • v.26 no.7
    • /
    • pp.575-580
    • /
    • 2003
  • The present study was designed to develop a microencapsulated L-ascorbic acid and iron that could be used to fortify milk and to determine the sensory properties of milk fortified with microencapuslation. Coating material was medium-chain triacylglycerol (MCT), and selected core material was ferric ammonium sulfate and L-ascorbic acid. The highest efficiency of microencapsulation was 95.0% in the ratio of 15:1 as coating to core material. Ascorbic acid release was increased sharply up to 5 d storage as 6.5%. TBA value was the lowest when both capsulated iron and ascorbic acid were added during 12 d storage, compared with other treatments. In sensory analysis, most aspects were not significantly different between control and capsulated ascorbic acid fortified milk at 5 d storage. The present study indicated that the use of microencapsulated ascorbic acid with MCT is effective for fortifying milk. In addition, these results suggest that acceptable milk products can be prepared with microencapsulated ascorbic acid and iron.

Analysis of ascorbic acid contents in raw, processed, and cooked foods by HPLC (HPLC를 이용한 식품의 ascorbic acid 함량의 분석과 조리에 의한 변화)

  • 계승희
    • Journal of the Korean Home Economics Association
    • /
    • v.31 no.4
    • /
    • pp.201-208
    • /
    • 1993
  • The ascorbic acid contents of 101 food items were analyzed by HPLC to provide database to estimate dietary intakes of ascorbic acid of Korean. Foods with high contents of ascorbic acid were green vegetables, citrus fruits, strawberry, kiwi, and fruit juices. This analysis data of ascorbic acid contents in some food items showed significant deviations compared with other Food Composition Table. Ascorbic acid content in soups were lower than those of raw foods by about 57%. The ascorbic acid contents in blanched or seasoned after blanching vegetables and boiled or steamed meals turned out to be decreased by about 52.3% and 47.5%, respectively, but the degrees were varied with the kind of foods as well as cooking methods. The ascorbic acid intakes from 18 most frequently consumed meals in Korea were determined to be about 1/2 of Food Composition Table according to this analysis data. The results showed the importance of accurate food database in assessing nutrient intake levels of population.

  • PDF

Percutaneous Absorption of Ascorbic acid Dipalmitate in Various Ointment Bases (아스코르빈산 디팔미테이트의 경피흡수에 미치는 연고기제의 영향)

  • Moon, Yong-Koo;Lee, Wan-Ha
    • Journal of Pharmaceutical Investigation
    • /
    • v.21 no.2
    • /
    • pp.97-101
    • /
    • 1991
  • Six different O/W cream bases containing 4% ascorbic acid dipalmitate and two different O/W cream bases containing 1% ascorbic acid were prepared. Percutanceous absorption of ascorbic acid as well as safety were determined using rabbits. The stability of the creams was also tested at room temperature. Ascorbic acid concentrations in urines varied depending on the characteristics of cream bases used. The absorption of ascorbic acid was increased and sustained with the cream bases containing branched chain esters of fatty acid instead of natural oils used currently. The excretion level of ascorbic acid in urine was high with the cream base including nonionic surfactants and a small quantity of natural oils. The creams containing nonionic surfactants showed excellent stability, while those containing anionic surfactants were not stable in terms of pH, odor and coloring test at room temperature during six months. But, the two creams containing ascorbic acid were unstable. All the cream bases tested showed good safety.

  • PDF