• Title, Summary, Keyword: ascites

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Development of Chromatographic Downstream Processing for the Purification of Monoclonal Antibody from Ascites Fluid: Part II Use of Single Hydroxylapatite Chromatographic Step (생쥐 복수로부터의 단세포군 항체분리를 위한 크로마토그라피 분리정제 방법의 개발 Part II. 히드록실아파타이트 크로마토그라피 단일 단계만의 사용)

  • Ahn, I.S.;Park, C.Y.
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.269-272
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    • 1989
  • In order to obtain monoclonal antibody from ascites fluid at sufficiently high purity using a single hydroxylapatite chromatography (HA) a further optimization on its operating variables was carried out. By adjusting the pH of the eluent, the sodium phosphate buffer, to 6.0 from 6.8 and adding CaCl$_2$to 1 mM at the column inlet, the elution molarities (M$_{elu}$) for the desired monoclonal antibody and contaminating proteins can be distinguished from each other with enough resolution. Previously these two groups of proteins co-eluted at the same time at pH 6.8 and without CaCl$_2$. This sin81e step hydroxylapatite chromatography yields the desired antibody pure enough for diagnostic use.

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Clinical Case of Liver Cirrhosis, with Herbal Medicine, Acupuncture, and Moxibustion Treatment (간경변 환자의 한방치험례)

  • Shin, Woo-Jin
    • The Journal of Internal Korean Medicine
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    • v.30 no.1
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    • pp.241-248
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    • 2009
  • Object : Liver cirrhosis is a disease of the liver in which normal cells are replaced by scar tissue. The purpose of this case is to report the improvement of liver cirrhosis after herb-med, acupuncture & moxibustion complex therapy. Methods : We provided herb-med, acupuncture & moxibustion complex therapy to a patient who suffered from ascites and fatigue. We examined LFT & Child-Pugh class to evaluate the effectiveness of oriental treatment. Result and Conclusion : We observed that herb-mod. acupuncture & moxibustion complex therapy decreased symptoms of liver cirrhosis and improved general condition of a patient who suffered from ascites and fatigue. In the LFT result, Child-Pugh class was improved.

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Anti-tumor Activity of Acanthospermum hispidum DC on Dalton Ascites Lymphoma in Mice

  • Rajendran, N.N.;Deepa, N.
    • Natural Product Sciences
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    • v.13 no.3
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    • pp.234-240
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    • 2007
  • The present study investigated the anti-tumor activity of ethyl acetate extract of Acanthospermum hispidum DC against daltons ascites lymphoma in mice. The extract was prepared by cold maceration with ethyl acetate for 3 - 7 days and evaporated in vacuum to dry. (Yield : 14.2 g, 1.42% w/w). The extract was fractionated by column chromatography by using gradient elution technique and the diterpenes fraction isolated (0.649 g). Both extract and the fraction were administered as oral suspension with tween 20 in water to tumor bearing mice (DAL) and changes in dead cell count, histopathology of tumor cells, hematological parameters and median survival time (MST) were examined and compared with that of tumor control or 5-Fluorouracil (5-FU). The results indicate that both ethyl acetate extract and fraction possess anti-tumor activity. The study suggests that Acanthospermum hispidum DC seems promising as a source of diterpenes for potential anti-tumor activity.

Effect of KEUMSUNYEON on Lipolytic Action of Toxohormone-L from Cancerous Ascites Fluid (Toxohormone-L의 지방질분해 작용에 미치는 금선연의 영향)

  • 이성동;취전척도
    • The Korean Journal of Food And Nutrition
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    • v.2 no.2
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    • pp.27-30
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    • 1989
  • This study was devised to observe an inhibitory reaction toward an lipolytic action of toxohormone-L from KEUMSUNYEON powder. Toxohormone-L is known to be a lipolytic factor that was purified from the ascites fluid of sarcoma 180-bearing mice and of patients with hepatoma. KEUMSUNYEON powder was found to inhibit toxohormone-L induced lipolysis at its concentration of 0.5 mg/ml.

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Effect of Acidic Polysaccharide of Korean Red ginseng on Lipolytic Action of Toxohormone-L from Canceroils Ascites Flilid (고려인삼의 산성다당체 성분이 암독소 호르몬-L의 지방질 분해작용에 미치는 영향)

  • 이성동;오꾸다히로미찌
    • Journal of Ginseng Research
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    • v.14 no.1
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    • pp.67-73
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    • 1990
  • Toxohormone-L is a lipolytlc factor, found in ascites fluid of sarcoma 180-bearing mice and of patients with hepatoma. A substance that inhibited the lipolytic action of Toxohormone-L was isolated from Korean red ginseng powder. This substance had a pectin-like a-1,4-polygalacturonan backbone with some acetoxyl groups, and so was an acidic polysaccharide. Acidic polysaccharide was found to inhibit significantly toxohormone-L-induced lipolysis at its concentration of 10$\mu\textrm{g}$/ml.

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Effect of Acidic Polysaccharides of White Ginseng on Lipolytic Action of Toxohormone-L from Cancerous Ascites Fluid (고려백삼의 산성다당체 성분이 암독소호르몬-L의 지방질분해에 미치는 영향에 관하여)

  • Lee, Seong-Dong;Jeon, Jung-Chi
    • The Korean Journal of Food And Nutrition
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    • v.3 no.1
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    • pp.9-12
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    • 1990
  • Toxohormone-L is a lipolytic factor, found in ascites fluid of sarcoma 180-bearing mice and of patients with hepatoma. A substance that inhibited the lipolytic action of toxohormone-L was isolated from white ginseng powder. This substance was an acidic polysaccharides It inhibited toxohormone-L-induced lipolysis in a dose dependent manner at concentrations higher than 100g/ml.

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A Case of the Hepatic Hydrothorax in the Absence of Ascites Confirmed by Tc-99m Macroaggregated Serum Albumin Scan (Tc-99m MAA scan으로 증명된 복수를 동반하지 않은 간성수흉증 1예)

  • Chung, Jae-Ho;Seo, Hye-Sun;Park, Moo-Suk;Ko, Won-Ki;Lee, Sun-Min;Yang, Dong-Gyoo;Ahn, Chul-Min;Kim, Sung-Kyu;Lee, Won-Young
    • Tuberculosis and Respiratory Diseases
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    • v.50 no.1
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    • pp.117-121
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    • 2001
  • Pleural effusion due to hepatic cirrhosis with ascites is well known, although hepatic hydrothorax in the absence of ascites is a rare condition, the pathogenesis of which is still unknown. We report a case of hepatic hydrothorax without ascites confirmed by the intraperitoneal injection of Tc-99m mecroaggregated serum albumin (Tc-99m MAA) that demonstrated the passage of Tc-99m MAA into the right pleural cavity.

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Inhibition of Sarcoma-180 Cell-induced Mouse Ascites Cancer by Astaxanthin-containing Egg Yolks (Sarcoma-180 Cell로 유발한 Mouse 복수암에 대한 Astaxanthin 함유 난황의 효과)

  • 하영래;이상호;박철우;박경아;이영춘;최의성
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.1
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    • pp.163-167
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    • 1998
  • Anticarcinogenic activity of astaxanthin-contatining egg yolk(designate AEY) was investigated for mouse ascites carcinogenesis induced by mouse Sarcoma-180(S-180) cells. Female ICR mice(8mice/treatment, 7∼8weeks of age, 25±1g) were injected, i.p. with S-180 cells(1×107cell/ml PBS). Two days later, each mouse was given 0.1ml PBS containing AEY(10, 25 or 50ug/g body weight) or control egg yolk (CEY; 50ug/g body weight) every other day for 7 times. Control mice were only given 0.1ml S-180 cells and 0.1ml PBS. Mice treated with 25ug/g body weight of AEY showed 24.8 days of life, which was equivalent to 138% of control mice's life(180.0 dyas). Based on dose-dependant experiment of AFY, mice treated with 10ug/g body weight showed slightly longer life(19.4 days) relative to mice treated with control mice, and mice treated with 50ug/g body weight exhibited 21.9 days of life. Mice treated with any dose of AEY exhibited longer life than mice with CEY 50ug/g body weight. Body weight of mice treated with AEY was reduced relative to that of control mice CEY-treated mice. These results suggest than AEY inhibits the carcinogenesis of mouse ascites induced by S-180 cells.

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Chunggangunbi-tang-gamibang's Effect on Alcoholic Liver Cirrhosis with Ascites (청간건비탕가미방(淸肝健脾湯加味方)으로 복수(腹水)를 동반한 알코올성 간경변(肝硬變) 환자(患者) 치험 1례)

  • Jeong, Eun-Woo;Cho, Yoon-Sung;Kim, Hyun-Sik;Kim, So-Yeon;Lee, Sang-Kwan
    • Herbal Formula Science
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    • v.15 no.2
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    • pp.161-169
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    • 2007
  • We applied Chunggangunbi-tang-gamibang to a 64 year-old male patient who had alcoholic liver cirrhosis with ascites. Chunggangunbi-tang-gamibang has been used to treat alcoholic liver disease and because of its beneficial effects. The patient with the disease expected several symptoms induced by Alcoholic liver cirrhosis, and we diagnosised differentiation of symptoms, using Chunggangunbi-tang-gamibang while removing dampness through diuresis and invigorating the spleen. After medication we could find remarkable effects on clinical symptoms, blood test results and abdomen ultrasonographic images. From this case, it is thought that using Chunggangunbi-tang-gamibang is very effective to improve the symptom of alcoholic liver cirrhosis with ascites.

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Port-Site Metastases and Chimney Effect of B-Ultrasound-Guided and Laparoscopically-Assisted Hyperthermic Intraperitoneal Perfusion Chemotherapy

  • Ba, Ming-Chen;Long, Hui;Zhang, Xiang-Liang;Gong, Yuan-Feng;Yan, Zhao-Fei;Wang, Shuai;Tang, Yun-Qiang;Cui, Shu-Zhong
    • Yonsei Medical Journal
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    • v.58 no.3
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    • pp.497-504
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    • 2017
  • Purpose: $CO_2$ leakage along the trocar (chimney effect) has been proposed to be an important factor underlying port-site metastasis after laparoscopic surgery. This study aimed to test this hypothesis by comparing the incidence of port-site metastasis between B-ultrasound-guided and laparoscopically-assisted hyperthermic intraperitoneal perfusion chemotherapy (HIPPC). Materials and Methods: Sixty-two patients with malignant ascites induced by gastrointestinal or ovarian cancer were divided into two groups to receive either B-ultrasound-guided or laparoscopically-assisted HIPPC. Clinical efficacy was assessed from the objective remission rate (ORR), the Karnofsky Performance Status (KPS) score, and overall survival. The incidence of port-site metastasis was compared between the two groups. Results: Patients in the B-ultrasound (n=32) and laparoscopy (n=30) groups were comparable in terms of age, sex, primary disease type, volume of ascites, and free cancer cell (FCC)-positive ascites. After HIPPC, there were no significant differences between the B-ultrasound and laparoscopy groups in the KPS score change, ORR, and median survival time. The incidence of port-site metastasis after HIPPC was not significantly different between the B-ultrasound (3 of 32, 9.36%) and laparoscopy (3 of 30, 10%) groups, but significantly different among pancreatic, gastric, ovarian, and colorectal cancer (33.33, 15.79, 10.00, and 0.00%, p<0.001). Conclusion: The chimney effect may not be the key reason for port-site metastasis after laparoscopy. Other factors may play a role, including the local microenvironment at the trocar site and the delivery of viable FCCs (from the tumor or malignant ascites) to the trauma site during laparoscopic surgery.