• Title, Summary, Keyword: antiviral agent

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Evaluation of Transdermal Patch for Antiviral Agent (항바이러스제 Transdermal Patch의 제조와 평가)

  • Choi, Seung-Ho;Kim, Johng-Kap
    • YAKHAK HOEJI
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    • v.34 no.6
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    • pp.429-433
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    • 1990
  • Transdermal patches and polymer membrane were prepared and evaluated for their ability to antiviral agent in vitro. The membrane perpared with styrene and HEMA by 0.5 and 10% of styrene composition. And the transdermal patches were fabricated with this membrane and silastic silicone sheeting. The antiviral agents used were ACV, BVDU and FEAU. The higher HEMA content membranes exhibited relatively high release rate of each drug. Permeation was enhanced by increasing of drug concentration. The parameters of each drug in diffusion experiment with styrene-HEMA membrane were investigated.

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Construction of a Hammerhead Ribozyme that Cleaves Rice Black-Streaked Dwarf Virus RNA (흑조위축병 바이러스 RNA를 절단하는 망치머리형 라이보자임의 제작)

  • Kim, Ju-Kon;Sohn, Seong-Han;Lee, Sug-Soon;Hwang, Young-Soo;Park, Jong-Sug
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.522-527
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    • 1995
  • To develop an antiviral agent for the rice black-streaked dwarf virus (RBSDV), a hammerhead type ribozyme, which has a potential target site on the genome segment 3, was designed. Oligonucleotides for the ribozyme and its substrate were synthesized, annealed, and cloned into a plasmid pBluescript II KS(+). Ribozyme and substrate RNAs were then synthesized by in vitro transcription with $T_3$ RNA polymerase, obtaining RNAs in expected size, 193 and 182 nucleotides, respectively. The substrate RNA was efficiently cleaved into two fragments when incubated with the ribozyme at $55^{\circ}C$, while the cleavage was not detected at $37^{\circ}C$. In addition, the segment 3 RNA of RBSDV was also cleaved into two fragments by the same ribozyme at $55^{\circ}C$. Taken together, our results demonstrated that the hammerhead ribozyme has an in vitro endonucleolytic activity and may be used as an antiviral agent in transgenic plants.

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Synthesis and Antiviral Activity of Novel trans-2,2-Dimethyl Cyclopropyl Nucleosides

  • Kook, Min-Chul;Choi, Bo-Gil
    • Archives of Pharmacal Research
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    • v.26 no.11
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    • pp.887-891
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    • 2003
  • Novel trans-2,2-dimethylcyclopropyl nucleosides were synthesized as potential antiviral agents. The key intermediate, 3, was synthesized via five steps from ethyl chrysanthemate and condensed with purine bases using the Mitsunobu reaction to give six cyclopropyl nucleosides. These synthesized nucleosides did not show any significant antiviral activity against HSV-1, HSV-2, EMCV, Cox B3, or VSV, at concentrations up to 100 $\mu M$.

Synthesis and Antiviral Activity of Novel Methylene Cyclopropyl Nucleosides

  • kwak, Eun-Yee;Hong, Joon-Hee;Lee, Chong-Kyo;Choi, Bo-Gil
    • Archives of Pharmacal Research
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    • v.23 no.6
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    • pp.559-563
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    • 2000
  • Novel exomethylene cyclopropyl nucleosides were synthesized as potential antiviral agents. The key intermediate 5 was synthesized in 4 steps, from Feists acid 1 and was condensed with purine derivatives by the $S_N2$ type reaction to give some cyclopropyl nucleosides. The synthesized nucleosides did not showed any significant antiviral activity against HSV-1, HSV-2, HCMV, HIV-1, HIV-2, and HBV up to 100 $\mu\textrm{m}$.

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Effect of Azone on Penetration of Antiviral Agents through Hairless Mouse Skin (무모(無毛) 마우스의 피부를 통한 항바이러스제의 투과와 Azone의 영향)

  • Choi, Seung-Ho;Kim, Johng-Kap
    • Journal of Pharmaceutical Investigation
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    • v.20 no.4
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    • pp.205-208
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    • 1990
  • 1-Dodecylazacycloheptan-2-one (Azone) is a new agent that enhances the percutaneous penetration of a number of different chemicals. BVDU and FEAU were evaluated for their potential efficacy in the treatment of cutaneous herpes simplex virus infections by in vitro studies through hairless mouse skin. This study demonstrates the value of penetration enhancing agent (Azone) and the need for a predictable evaluations in the development of topical antiviral agents.

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Synthesis and Antiviral Evaluation of 1'-Branched-5'-Norcarbocyclic Adenosine Phosphonic Acid Analogues

  • Oh, Chang-Hyun;Yoo, Kyung-Ho;Hong, Joon-Hee
    • Bulletin of the Korean Chemical Society
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    • v.31 no.9
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    • pp.2473-2478
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    • 2010
  • Novel 1'-methyl-5'-norcarbocyclic adenosine phosphonic acid analogues were synthesized using an acyclic stereoselective route from commercially available 3,3-diethoxy-propan-1-ol 4. The synthesized nucleoside phosphonate 19 and phosphonic acid 21 were subjected to antiviral screening against various viruses.

Research Trend of Antiviral Natural Products for Companion Animal (천연물 유래의 반려동물 항바이러스활성물질 연구 동향)

  • Kang, Byeong Ku;Yang, Seo Young;Kim, Young Ho
    • Korean Journal of Pharmacognosy
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    • v.50 no.1
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    • pp.1-10
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    • 2019
  • Recently, companion animal culture has grown rapidly and mature, raising interest in preventing and treating animal diseases. In particular, viral infection was a serious threat to companion animal health because there was no proper antiviral drugs. Synthetic antiviral drugs have limitations such as low efficiency, toxicity, and occurrence of resistant viruses. Therefore, attempts to find new anti-viral drugs from natural sources have continued. This review focused on the natural products and active substances that exhibit antiviral activity against three viruses: canine distemper virus (CDV), canine parvovirus (CPV), and feline calicivirus (FCV) that cause fatal diseases in dogs and cats. Natural plant extracts, flavonoids, polysaccharides, alkaloids and saponins showed antiviral activity with various mechanisms and differences in activity depending on the structure. Especially, quercetin and epigallocatechin-3-gallate (EGCG) showed antiviral activity through a multi-mechanism that interferes with the attachment and penetration stages of the virus and inhibits the viral polymerase within the cell. Some natural plant extracts showed a virucidal activity and showed the potential effect as a preventative agent to prevent the viral infection. This review is expected to provide research trend on the development of antiviral natural products for companion animals.

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Antiviral Activity of the Plant Extracts from Thuja orientalis, Aster spathulifolius, and Pinus thunbergii Against Influenza Virus A/PR/8/34

  • Won, Ji-Na;Lee, Seo-Yong;Song, Dae-Sub;Poo, Haryoung
    • Journal of Microbiology and Biotechnology
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    • v.23 no.1
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    • pp.125-130
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    • 2013
  • Influenza viruses cause significant morbidity and mortality in humans through epidemics or pandemics. Currently, two classes of anti-influenza virus drugs, M2 ion-channel inhibitors (amantadin and rimantadine) and neuraminidase inhibitors (oseltamivir and zanamivir), have been used for the treatment of the influenza virus infection. Since the resistance to these drugs has been reported, the development of a new antiviral agent is necessary. In this study, we examined the antiviral efficacy of the plant extracts against the influenza A/PR/8/34 infection. In vitro, the antiviral activities of the plant extracts were investigated using the cell-based screening. Three plant extracts, Thuja orientalis, Aster spathulifolius, and Pinus thunbergii, were shown to induce a high cell viability rate after the infection with the influenza A/PR/8/34 virus. The antiviral activity of the plant extracts also increased as a function of the concentration of the extracts and these extracts significantly reduced the visible cytopathic effect caused by virus infections. Furthermore, the treatment with T. orientalis was shown to have a stronger inhibitory effect than that with A. spathulifolius or P. thunbergii. These results may suggest that T. orientalis has anti-influenza A/PR/8/34 activity.

Screening of Microorganisms having Antiviral Activity against HIV Virus from Soil (토양으로부터 HIV 바이러스에 항바이러스 활성을 나타내는 미생물의 탐색)

  • 김성욱;이상한;정태숙;문석식;이종교;이항우;이원영;복성해
    • Microbiology and Biotechnology Letters
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    • v.21 no.5
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    • pp.399-405
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    • 1993
  • For screening of the antiviral agent from soil, about 520 strains of microorganisms were evaluated for their antiviral activity, About 6.9% of strains showed more than 95% antiviral activity against Herpes Simples Virus (HSV)-1. Two strains among 30 strains active against HSV-1 virus showed a quite strong activity against human immunodeficiency virus.

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Synthesis and Antiviral Evaluation of Novel Pyrimidine Thioapionucleosides (신규 피리미딘 티오에피오 뉴크레오사이드의 합성 및 항바이러스 약효검색)

  • Lee Rae-Sang;Hong Joon-Hee;Ko Ok-Hyun
    • YAKHAK HOEJI
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    • v.50 no.2
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    • pp.65-69
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    • 2006
  • Novel 4'-hydroxymethyl branched thioapiosyl nucleosides were synthesized in this study. The introduction of hydroxymethyl group in the 4'-position was accomplished by a [3,3]-sigmatropic rearrangement. Thioapiosyl sugar moiety was constructed by sequential ozonolysis, reduction and cyclization. The pyrimidine nucleosidic bases (uracil, 5-fluorouracil, 5-iodouracil, 5-chlorouracil, 5-bromouracil) were efficiently coupled by Vorbruggen glycosyl condensation procedure (per-silyated base and TMSOTf). The antiviral activities of the synthesised compounds were evaluated against the HIV-1, HSV-1, HSV-2 and EMCV 5-Iodouracil 18 showed weak antiviral activity against HSV-1 $(EC_{50}=30.7{\mu}M)$.