• Title, Summary, Keyword: antitumor

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Experimental Studies on Antitumor Effect and Immune Responses of Bunsimgieum (분심기음의 항암작용 및 면역기능에 관한 연구)

  • Kim Jin-Sung;Yoon Sang-Hyub;Ryu Bong-Ha;Ryu Ki-Won;Yeo Dae-Won
    • The Journal of Internal Korean Medicine
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    • v.24 no.2
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    • pp.315-328
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    • 2003
  • This study was performed to investigate the effect of Bunsimgieum on antitumor effect after sarcoma-180 cells transplantation into peritoneal cavity or left groin and immune responses on the depressed immunity induced by methotrexate in mice. The Bunsimgieum extract of 10mg/kg was orally administered 14 days for antitumor effects and 21 days for immune responses. 50% inhibitory concentration($IC_{50}$) of SUN-1, SUN-C4, and SUN-396 cancer cell, mean sunvival days and body weight of tumor bearing mice, and growth of tumor mass for antitumor effect; delayed type hypersentivity, hemagglutinin titer, hemolysis titer, rosette forming cells, natured killer cell activity, lymphocyte transformation, productivity of interleukin-2, and phagocytic activity for their immune responses were measured in ICR mice. Significance in antitumor effect is noted in the enlongation of mean life days and inhibition of tumor growth(p<0.01, respectively). Significance of immune responses is also noted in hemolysis titer, lymphocyte transfumotion, IL-2 productivity, phagocytic activity, and natural killer cell activity at E/T ratio 100:1(p<0.01, respectively). Significant in rosette cell formation was seen at dosage of 20mg/kg(p<0.01). However, Difference of body weight as antitumor effect, delayed type hypersensitivity, and hemagglutinin titer were not shown significantly. According to the above results, it could be suggested that Bunsimgieum has prominent antitumor and immunity enhancing effect.

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Development of Anticancer Agents from Korean Medicinal Plants. Part 6. -Cytotoxic Activity of the Ethyl Acetate Soluble Fraction of Lonicerae flos against Human Oral Epitheloid Carcinoma Cells- (한국산 생약으로부터 항암물질의 개발(제6보). -금은화 Ethyl Acetate 가용성 분획의 인체 구강유상피암종세포에 미치는 세포독성작용-)

  • Han, Du-Seok;Baek, Kyong-Hyun;Kim, Young-Ok;Choi, Kyu-Eun;Kwag, Jung-Suk;Baek, Seung-Hwa
    • Korean Journal of Pharmacognosy
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    • v.29 no.1
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    • pp.22-27
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    • 1998
  • This study was carried out to develop antitumor agents based on effects of the ethyl acetate soluble fraction of the methanolic extract of Lonicerae flos on human oral epitheloid carcinoma cells. Human oral epitheloid carcinoma cells were cultured in RPMI-1640 media containing 10% fetal bovine serum, antibiotic, and fungizone. After incubation for 24 hrs, the cells were treated with A, B, C, D, and E fractions for 48hrs under the same condition. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide), NR (Neutral red) and SRB (Sulforhodamine B protein) assay were performed. The light microscopic findings were observed by inverted microscope. In MTT assay, fraction B was shown significant antitumor activity (P<0.001), fraction E was shown significant antitumor activities (P<0.05), but the other fractions were not shown. In NR assay, fraction B was shown significant antitumor activity (P<0.001). In SRB assay, fractions B was shown significant antitumor activities (P<0.01). fractions A and D were shown significant antitumor activities (P<0.05). but the other fractions were not shown. In light microscopy. the fraction B of the ethyl acetate soluble fraction of the methanolic extract of Lonicerae flos showed the highest antitumor activity. These finding suggested that fraction B possessed the most antitumorous agent.

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띠미로 버섯 추출물의 항암효과

  • 양규호;양정희;류병호
    • Microbiology and Biotechnology Letters
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    • v.25 no.2
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    • pp.178-182
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    • 1997
  • This study was carried out particutarly focusing on the antitumor effects of extracts obtained from mushroom, Daedalea dickinsii against Hela cell, Hep $G_{2}$, cell, L 929 cell and Sarcoma-180. Antitumor effect of hot water extracts obtained from Daedalea dickinsii against Hela cell showed at the highest level of 70% when adiministrated at the concentration of 1.5 mg/100 ml, however Hep $G_{2}$, cell inhibited 40% at the same concentration of hot water extracts. Antitumor effects of methanol extract of Daedalea dickinsii against Hela cell indicated at the highest level of 60% when 4 mg/ml was administerated. Antitumor effect of hot water extract of Daedalea dickinsii inhibited 51.03% against L929 cell. The solid tumor sarcoma-180 growth inhibition of methanol extract of Deadalea dickinsii inhibited 45.67% when administrated at the concentration of 60mg/kg, and the life prolongation was higher 30.88% than that control group.

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Screening of Antitumor Activity from the Crude Drugs in Korea (국내 생약자원으로부터의 항종양효과의 검색)

  • Ryeom, Kon;Choi, Byung-Don
    • Korean Journal of Pharmacognosy
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    • v.31 no.1
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    • pp.16-22
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    • 2000
  • These studies were designed to determine the potential cytotoxic activity of MeOH extracts of 65 crude drugs against leukemia L1210 and $P388D_1$, cell line in vitro, of which 25 samples were selected. The n-BuOH extracts of 25 samples were measured using the same method and nine were selected. These samples were measured for the potential antitumor activity against $P388D_1$, for life span in vivo, and against sarcoma 180 for tumor weight. On the basis of results, Notoginseng Radix, Anemarrhenae Rhizoma, Albizziae Cortex, Portulacae Herba, Bupleuri Radix were evaluated the effective plant on the antitumor activity. In addition, the mixture of Notoginseng Radix and Albizziae Cortex was evaluated to enhance the antitumor activity in vivo.

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Studies on Antitumor Components of Flammulina velutipes of Korea (II) - Production of Antitumor Component of Flammulina velutipes by Submerged Culture - (팽나무버섯의 항암(抗癌) 성분(成分)에 관한 연구(硏究)(제(第)2보(報)) - 액내(液內) 배양(培養)에 의한 항암(抗癌) 성분(成分)의 생성(生成) -)

  • Woo, Myoung-Sik
    • The Korean Journal of Mycology
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    • v.11 no.4
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    • pp.147-150
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    • 1983
  • To investigate a possibility of producing the antitumor component by shake culture method, the mycelia of Flammulina velutipes were cultured in flasks on a shaker at $26{\sim}28^{\circ}C$ at 180 rpm for seven days. The extract of the mycelia was concentrated under vacuum. The precipitate obtained by adding a three-fold volume of ethanol was centrifugated and freeze-dried after dialysis. The fraction was tested against sarcoma 180 in the mice. The inhibition ratio of the fraction against the tumor was 68.0% at the dose of 20 mg/kg/day for the period of ten days and the tumors in three of the mice were completely regressed. The results showed, therefore, that the antitumor component was produced by the shake culture method.

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Antitumor Components of Korean Basidiomycetes

  • Kim, Byong-Kak;Kim, Ji-Hyun;Kim, Ha-Won;Choi, Eung-Chil
    • Korean Journal of Pharmacognosy
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    • v.17 no.1
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    • pp.39-48
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    • 1986
  • To find antitumor components in the shake-cultured mycelia of Volvariella bombycina, the mycelia were extracted with hot water. After the extract was dialyzed and freeze-dried, a protein-polysaccharide fraction was obtained and examined for antitumor activity against the solid form of sarcoma 180 in ICR mice. It showed 60.3% inhibition ratio at a dose of 20mg/kg/day for 10 days. It was found to consist of a polysaccharide moiety and a protein moiety. After gel filtration on Sepharose 4B, Fraction B was obtained and showed the highest inhibition ratio of 71.1%. When the antitumor component was examined for immunopotentiating activity, it was found to increase the macrophage accumulation in the peritoneal cavity as well as the antibody production of the spleen cells of the mice.

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Antitumor Constituents of Polyporus giganteus

  • Kim, Byong-Kak;Shim, Mi-Ja;Kim, Ha-Won;Woo, Myoung-Sik;Choi, Eung-Chil
    • Archives of Pharmacal Research
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    • v.10 no.3
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    • pp.179-183
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    • 1987
  • To investigate antitumor constituents of higher fungi, the carpophores of Polyporus giganteus Pers. ex. Fr. (81 g, dry weight) which were collected in Indiana, U. S. A. were examined for antitumor activity. Two protein-bound-polysaccharide fractions (I and II) were prepared from the hot water extract and one fraction (III) from the 0.1 N NaOH extract of the carpophores. The antitumor effect of each fraction was tested against sarcoma 180 implanted subcutaneously in female ICR mice. Of three fractions, Fraction II showed 85.2% inhibition ratio at the dose of 20 mg/kg/day for 10 days and was named gigantan. Gigantan was found to contain 59% polysaccharide and 27% protein. Its polysaccharide moity was a heteroglycan that consisted of mainly glucose (89.3%), galactose (7.7%), minaose (2.0%) and fructose (1.0%).

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Naphthazarin Derivatives (Ⅶ): Antitumor Action against ICR Mice Bearing Ascitic S-780 Cells

  • Song, Gyu-Yong;Kim, Yong;You, Young-Jae;Cho, Hoon;Ahn, Byung-Zun
    • Archives of Pharmacal Research
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    • v.24 no.3
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    • pp.190-193
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    • 2001
  • Various analogues of 5,8-dimethoxy-1,4-naphthoquinone (DMNQ) such as 2- or 6-(1-hydroxyiminoalkyl)-DMNQs were prepared and evaluated for the antitumor action. (1 -Hydroxyiminoalkyl)-DMNQs derivatives expressed greater antitumor action than (1-hydroxyalkyl) - or acyl-DMNQ derivatives. Moreover, 6-(1-hydroxyiminoalkyl)-DMNQ derivatives expressed higher antitumor action than 2-substituted ones, suggestive of a steric effect. Some of 6-(1-propyloxyalkyl)-DMNQ derivatives with an alkyl group of butyl to octyl moiety showed T/C values of >400%

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Studies on Constituents of the Higher Fungi of Korea (XLVI) -Antitumor Components Extracted from the Cultured Mycelia of Lyophyllum decastes-

  • Kim, Hye-Ryoung;Shim, Mi-Ja;Kim, Jung-Woo;Kim, Ha-Won;Lee, Chong-Ock;Choi, Eung-Chil;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.15 no.2
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    • pp.61-73
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    • 1984
  • To investigate antitumor component of Lyophyllum decastes, the aqueous extract of its shake-cultured mycelia was subjected to antitumor test against sarcoma 180 cells implanted in ICR mice. The extract showed an inhibition ratio of 65.4% and was found to consist of a polysaccharide moiety and a protein moiety. After purification with DEAE-Sephadex A-50 ion exchange chromatography, Fraction D showed the highest inhibition ratio of 75.7%. The antitumor constituent was examined for immunoaccelerating activity and was found to increase macrophage accumulation in the peritoneal cavity and plaque forming cells of the spleen cells. It was named lyophyllan after the genus name.

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Synthesis and Antitumor Activity of Pt(ll) Complexes Containing Ethylenediamine (에칠렌디아민을 배위자로 한 백금(II) 착체의 합성과 항암효과)

  • Lee, Kyung-Tae;Jung, Jee-Chang;Roh, Young-Soo
    • Journal of Pharmaceutical Investigation
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    • v.24 no.4
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    • pp.245-250
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    • 1994
  • An attempt was made to develop new water-soluble antitumor Pt(ll) complexes containing ethylenediamine, and their structures were determined by infrared spectroscopy, $^{13}C$ nuclear magnetic resonance and elemental analysis. Their antitumor activities in vitro against L-1210, p-388 leukemia cells and M-14 melanoma cells were investigated and acceptable antitumor activity was found as compared with cisplatin.

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