• Title, Summary, Keyword: antitumor

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Purification and Characterization of the Antitumor Antibiotic from Streptomyces sp. YBE-316 (Streptomyces sp. YBE-316이 생산하는 항암성 항생물질의 정제 및 특성)

  • Park, Jae-Hong;Hahm, Byoung-Kwon;Bai, Dong-Hoon;Yu, Ju-Hyun
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.329-336
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    • 1995
  • For the development of new antitumor antibiotics produced by microorganisms, Streptomyces sp. YBE-316 was isolated from soil. The productivity of the antitumor antibiotic from Streptomyces sp. YBE-316 gradually increased after 60 hours, and was maximum after 100 hours after inoculation in growth medium (2.0% sucrose, 1.0% soybean meal, 0.1% K$_{2}$HPO$_{4}$, pH 7.0) at 30$\circ$C, 150 rpm, 5 NL/min by 30 l jar fermentor. This antitumor antibiotic was present only in mycelium, and stable in pH 5.0-10.0 for 20 minutes at 100$\circ$C. Antitumor and antibiotic activities were maintained at neutral pH, and heat stability was low. This antitumor antibiotic was soluble in methanol and ethanol, and insoluble in water, ethyl acetate, chloroform, and n-hexane. This antitumor antibiotic was sequentially purified by acetone extraction from mycelium, butanol extraction, and silica gel column chromatography. Antitumor activity was low against most tested cell lines, but antibiotic activity was high and low against yeasts and bacteria, respectivelv. The visualization test showed that this antitumor antibiotic had higher hydroxyl, ketone, amino, carboxyl groups, and sugar(s) in its structure. Instrumental analyses showed that this antitumor antibiotic was a pentaene in polyene class antibiotics. In pentaene class antibiotics, this was considered as an eurocidin or capacidin type antibiotics. The molecular weight of this antitumor antibiotic was higher than 683.0 daltons, and this antitumor antibiotic might be glycosylated by other sugar(s), instead of mycosamine or perosamine, an amino sugar.

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Effects of Antitumor Polysaccharides from Forsythia Corea on the lumune Function (I)

  • Moon, Chang-Kiu;Park, Kwang-Sik;Lee, Soo-Hwan;Ha, Bae-Jin;Lee, Byeong-Gon
    • Archives of Pharmacal Research
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    • v.8 no.1
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    • pp.31-38
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    • 1985
  • Polysaccharide fractions were prepared from Forsythia Corea by several different extraction schedules. The fractions obtained were designated as ForCo A, ForCo B and ForCo C respectively. ForCo C was further purified through Sephadex G 200 column chromatography and obtianed two subfractions (Coreana A, Coreana B). ForCo showed marked antitumor activity against sarooma 180 and its activity was dose-dependent. Coreana A, purified from ForCo C, showed somewhat higher antitumor activity. ForCo C increased the number of circulating leucocytes and peritoneal exudate cells, but didn't show any significant effects on the phagocytic activity and total serum protein level. Chemical analysis showed that ForCo C was composed of glucose, galactose, xylose and arabinose. A close relationship between antitumor activities and polysaccharide contents waw observed. These results indicate strongly that antitumor active principle of Forsythia Corea is polysaccharide.

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Isolation and Identification of a Soil Actinomycetes YBE-316 Producing an Antitumor Antibiotic (항암성 항생물질을 생산하는 토양 방선균 YBE-316의 분리 및 동정)

  • Shin, Jin-E;Park, Jae-Hong;Bai, Dong-Hoon;Yu, Ju-Hyun
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.297-303
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    • 1995
  • Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

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Antimicrobial and Antitumor Photodynamic Effects of Phleichrome from the Phytopathogenic Fungus Cladosporium Phlei

  • So, Kum-Kang;Chun, Jeesun;Kim, Dae-Hyuk
    • Mycobiology
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    • v.46 no.4
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    • pp.448-451
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    • 2018
  • Fungal perylenequinones have photodynamic activity and are promising photosensitizers for photodynamic therapy (PDT). Here, we investigated the bactericidal and antitumor activities of phleichrome from the fungal perylenequinone family in vitro. Photodynamic bactericidal activity of phleichrome was analyzed by agar-well diffusion method under dark and illuminated conditions. The photodynamic antitumor activity of phleichrome was analyzed in MCF-7, HeLa, SW480, and HepG2 human cancer cell lines using in vitro cytotoxicity assays. Photodynamic bactericidal activities against Gram-negative and Gram-positive bacteria were species-specific. Antitumor activity against all tumor cell lines increased under the illuminated condition. Depending on the results of the analyses, Phleichrome has potential for further drug development related to its antibacterial and antitumor activities.

Study on the Antitumor Activity of Tripterygium Regelii Sprague (미역줄나무의 항암활성에 관한 연구)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.2
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    • pp.441-445
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    • 2005
  • Tripterygium regelii has been used as an oriental medicine, especially antiparasitic, anti-inflammatory and detoxifying agents in East asia. During our research to develop new antitumor agents from natural products, MeOH ext. and CH2Cl2 ext. of Tripterygium regelii showed the potent antitumor activity. In order to purify active compounds from Tripterygium regelii, activity-guided fractionation was carried out. Silica gel and RP-18 column chromatography for the active fraction led to the isolation of two compounds and their antitumor activities were studied. Those two compounds didn't show potent antitumor activity against human tumor cell lines. The structure of two compounds were determined by $^1H-NMR$, $^{13}C-NMR$, DEPT, $^1H-^{13}C$ COSY and IR spectrum. Compound I and Compound II were turned out to be Celastrol, and ${\beta}-sitosteryl-3-o-{\beta}-D-glucopyranoside$ respectively.

Synthesis of pt(II) Complexes Containing Diphosphines and Evaluation of Antitumor Activity (디포스핀류 배위자를 함유한 백금(II) 착체의 합성과 항암효과)

  • Noh, Young-Soo;Masahide, Noji
    • Journal of Pharmaceutical Investigation
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    • v.20 no.4
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    • pp.217-222
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    • 1990
  • New antitumor-active pt(II) complexes of trans-l-diamine cyclohexane containing diphosphines as a leaving group were synthesized. The structures of the pt(II) complexes were determined by analyzing the infrared and $^{31}P-nuclear$ magnetic resonance spectra. Antitumor activities of the pt(II) complexes were tested against murine leukemia $L_{l210}$ according to the protocol of the National Cancer Institute. All the pt(II) complexes Synthesized were antitumor-active. In particular, water-soluble $[pt(trans-l-dach) (DPPP)](NO_3)_2$ exhibited excellent antitumor activity, giving T/C % values of 341 and 356 respectively, each with four cured mice out of six at a dose of 25 mg/kg. These pt(II) complexes are considered to be worthy of further development.

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The Effectiveness of IL-12 Administration and Fusion on Tumor Antigen Sensitization Methods for Dendritic Cells Derived from Patients with Myelogenous Leukemia (골수성백혈병에서 배양한 수지상세포(Dendritic Cell)에 대한 종양항원 감작법으로 IL-12 첨가와 융합법의 효과)

  • Kim, Kee Won;Park, Suk Young;Hong, Young Seon
    • IMMUNE NETWORK
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    • v.4 no.1
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    • pp.38-43
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    • 2004
  • Backgroud: Immunotherapy using dendritic cells (DC) loaded with tumor antigens may represent a potentially effective method for inducing antitumor immunity. We evaluated the effectiveness of DC-based antitumor immune response in various conditions. Methods: DC were cultured from peripheral blood mononuclear cells (PBMNC) in myelogenous leukemia (ML) and lysates of autologous leukemic cells are used as tumor antigen. The effectiveness of interleukin-12 (IL-12) and CD40L (CD154) on the antigen presenting function of lysates-loaded DC was analyzed by proliferation, cytokine production, and cytotoxicity tests with activated PBMNC (mainly lymphocytes). For generating antigen-loaded DC, direct fusion of DC with ML was studied. Results: Antigen loaded DC induced significantly effective antitumor immune response against autologous leukemic cells. Administration of IL-12 on the DC based antitumor immune response showed higher proliferation activity, IFN-$\gamma$ production, and cytotoxic activity of PBMNC. Also, fused cell has a potent antitumor immune response. Conclusion: We conclude that lysates-loaded DC with IL-12 may be effectively utilized as inducer of antitumor immune reaction in ML and in vivo application with DC-based antitumor immunotherapy or tumor vaccination seems to be feasible.

Immunopotentiating effects and Antitumor activities of Sipjundaebo-tang (십전대보탕(十全大補湯)의 면역증강(免疫增强) 및 항암(抗癌) 효과(效果))

  • Choi, Seung-Hoon;Oh, Min-Suck;Song, Tae-Won;Nam, Ki-Yeul
    • Journal of Haehwa Medicine
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    • v.11 no.1
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    • pp.257-283
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    • 2002
  • Objectives : Sipjundaebo-tang is prepared by ten medical herbs that tone the blood and vital energy, and strengthen health. This prescription has long been used traditionally against anemia, anorexia, extreme exhausion and fatigue. The purpose of this thesis was to review the effects of Sipjundaebo-tang that are about immunopotentiation effects, antitumor activities and potentiation, detoxification of antitumor drugs Methods : We studied the research methods and results of experiments which were selected from Korean, Japaness and American theses were on the topic of immunopotentiation effects, antitumor activities and potentiation, detoxification of antitumor drugs from Sipjundaebo-tang Results and conclusions : Sipjundaebo-tang not only potentiates the effects of the combined use of chemotherapy, but also reduces and elimimate the immunotoxicity of antitumor drugs and radiotherapy. And it strengthen immunity and improve QOL, S/OH of cancer patients.

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Synthesis of 1-(Heterocyclic Substituted Anilino)-9H-Thioxanthon-9-ones and Their Antitumor Activity

  • Omar, Mahmoud-T.
    • Archives of Pharmacal Research
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    • v.20 no.6
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    • pp.610-619
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    • 1997
  • Some new 9H-thioxanthen-9-one incorporated into heterocyclic systems such as pyridone 8, pyrazoline 9, pyranone 11, iminopyrane 12, furopyrimidine 17, imidazothiazole 19, thiazole 21, triazine 24 and other related compounds through a para imminophenyl grouping at position-1 of the thioxanthenone ring were synthesized and tested as antitumor agents against L1210 leukemia in mice. Some of the new compounds showed considerable antitumor activity.

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