• Title, Summary, Keyword: antimicrobial activity

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Isolation and Antimicrobial Activity of Antimicrobial Substance Obtained from Leaf Mustard(Brassica juncea) (갓(Brassica juncea)의 항균물질의 분리 및 항균성)

  • 강성구
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.5
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    • pp.695-701
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    • 1995
  • The ethanol extract of leaf mustard(Brassica juncea) exhibiting high antimicrobial activities was fractionated in the order of hexane, chloroform, ethylacetate and butanol fractions to test antimicrobial activity. The highest antimicrobial activity for the bacteria tested was found in the ethylacetate fraction, but a lesser extent in the butanol fraction. In contrast to antimicrobial activity for the bacteria, both ethylacetate and butanol fractions showed weak antimicrobial activity for yeasts. Unknown compound A in the ethylacetate fraction which exhibited a strong antimicrobial activity was isolated by silica gel column chromatography and HPLC, and exhibited 9 times more antimicrobial activity than the ethylacetate fraction.

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Study on the Antimicrobial Activity of Impatiens balsamina(III) (봉선화 추출물의 항균성에 관한 연구(III))

  • 최석철;정진순
    • Textile Science and Engineering
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    • v.36 no.4
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    • pp.338-343
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    • 1999
  • To develop a natural antimicrobial dyed fabric, water extracts were prepared from Impatiens balsamina leaf and stem. Wool and silk fabrics dyed with Impatiens balsamina extract were tested for antimicrobial activities aganist Staphylococcus aureus KCTC 1928 (St. aureus) and Trichopyton mentagrophytes (T. mentagrophytes) which are used in antimicrobial test of fabrics. The results were as follows Antimicrobial activity of wool and silk dyed with Impatiens balsamina extract increased with of dyeing and concentration of dye bath. The dyed silk fabric had higher antimicrobial activity than the dyed wool fabric. The fabrics dyed with leaf extract had higher antimicrobial activity than those dyed with stem extract.

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ANTIMICROBIAL EFFECT OF ROOT CANAL CEMENTS ON STREPTOCOCCUS SANGUIS (수종 근관충전세멘트의 Streptococcus Sanguis에 대한 항균효과에 관한 실험적 연구)

  • Kwon, O-Yang;Yoon, Soo-Han
    • Restorative Dentistry and Endodontics
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    • v.12 no.1
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    • pp.77-84
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    • 1986
  • This study was designed to compare the antimicrobial effect of the several root canal cements and to determine the duration of their activity. After Strep. sanguis 9811 was streaked on the surface of BHI agar and Plain agar, PVC tubes filled with root canal cements were applied and cultured for 48 hours, aerobically. Following results were obtained, 1. All of the examined root canal cements had antimicrobial activity with varying degree. 2. The antimicrobial activity of N2 was larger than any other root canal cements. Nogenol and Tubliseal showed the lowest activity. ZOE, AH26, Procosol and PCA cement showed the moderate antimicrobial activity. (P>0.05) 3. At one day after mixing the root canal cements, all of the root canal cements except N2 and PCA cement showed greatly reduced antimicrobial activity. 4. At three days after mixing, PCA cement showed the reduced antimicrobial activity. N2 showed the reduced activity at 7 days after mixing.

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Purification of Antimicrobial Substance for the Extract from the Root Bark of Morus alba (상백피 추출물로부터 향균성 물질의 분리정제)

  • 박욱연;김신희;김지회;김용관;장동석
    • Journal of Food Hygiene and Safety
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    • v.10 no.4
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    • pp.225.1-230
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    • 1995
  • The ethanol extract from the root bark of Morus alba showed the strongest antimicrobial activity on the growth of almost all the tested microorganisms which were food-borne pathogens and food-related microorganisms. 1) In order to isolate and purify of antimicrobial substance extracted from the root bark of Morus alba, the antimicrobial substance from the ethanol extract which exhibited a strong antimicrobial activity was purified by solvent fractionation, silica gel column chromatography, TLC and HPLC. Among the fractions fractionated by 4 kind of solvents from the ethanol extract, the antimicrobial activity of ethyl acetate fraction had the strongest antimicrobial activity against B. subtilis. Unknown compound were isolated from the ethyl acetate fraction by silica gel column chromatography, TLC and HPLC and the compounds showed strong absorbance at 207, 217 and 285 nm, therefore, it was supposed to be a kinds of aromatic compound.

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A Study on Synthesis and Antimicrobial Activity of Rubber Mat (고무 매트의 합성 및 항미생물 활성에 관한 연구)

  • Kim, Ki-Jun;Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.21 no.4
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    • pp.327-334
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    • 2004
  • Cysteine-silver complexes were prepared and investigated the antimicrobial activity on rubber mat manufactured with waste rubber. We are exposed to harmful bacteria and fungi all the time. We manufactured antimicrobial mat to be imposed to mats that it can prevent generation of bacteria and microorganisms, and restrict their reproduction. Infection of medical devices causes significant morbidity and mortality. For aim of this study, we measured the antimicrobial mat manufactured with cysteine-Ag complex by CCD, FT-IR and NMR. The effect of mole ratio of cysteine-Ag complex on antimicrobial activity to bacteria and fungi is investigated. Reduction rate is evaluated using the Quinn method. Antimicrobial activity of complex on mole ratio 1:4 was effectively inhibited. The complexes had a better antibacterial activity than antifungal activity on rubber mat.

Antimicrobial Characterictics of Antimicrobial Agent (Antibiotics) and Reduction Effect on Mal-ordour. (항균제의 항균특성 및 악취제거 효과)

  • Shin, Choon-Hwan;Kim, Jong-Hyun;Han, Sun-Hong
    • Journal of Environmental Science International
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    • v.3 no.2
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    • pp.157-164
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    • 1994
  • Various antimicrobial agents are widely used for the purpose of antimicrobial process. We investigated antimicrobial activity and reduction efficiency of mal-ordour by the diphenyl ether compound (2,4,4'- trichloro -2'- hydroxy diphenyl ether) against Sraphylocom aureus(S.aureus and Proton vulgaris(p.vulgaris causing the mal-ordour, Especially, the diphenyl ether compound is not restricted to the regulation of water-contamination. In this research, we found that the optimum concentration of diphenyl ether compound was 1.5w% for both strains and antimicrobial expressions were c0.38t= 2.56 for S.aureus, c0.38t=2.67 for P.vulgaris. We found also that -OH group played the role of antimicrobial functional group. Lastly, reduction effect of mal-ordour was more than 90% for both strain at the optimum conditions. Key Words : antimicrobial agents, antimicrobial activity, reduction effect of mal-ordour, antimicrobial expression, antimicrobial functional group.

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Antimicrobial activity of sophorolipid

  • Kim, Gab-Jung;Kim, Young-Bum;Kim, Eun-Ki
    • 한국생물공학회:학술대회논문집
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    • pp.247-249
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    • 2000
  • Antimicrobial activity of sophorolipid produced by Candida bombicola ATCC 22214 was investigated, Minimum inhibitory concentration of sophorolipid to Propionibacterium acne and Bacillus subtilis were 0.5 ppm and 4 ppm, respectively. However no antimicrobial activity to E. coli was detected. Indicating the antimicrobial activity of sophorolipid only toward gram positive bacteria. Antifungal activity of sophorolipid was also detected in the hyphae growth assay for Botrytis cinerea. Malate dehydrogenase, a cytoplasmic enzyme, of B. subtilis was remarkably increased with the sophorolipid treatment, indicating the leakage of membrane by sophorolipid. This result shows the potentials of sophorolipid as mild, non-toxic antimicrobial agent.

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Antimicrobial Properties of Paper Treated with Acidic Liquid from Carbonized Rice Hull (왕겨초액 처리지의 항균 특성)

  • Min, Choon-Ki;Jo, Joong-Yeon;Shin, Jun-Sub;Lee, Se-Eun
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.43 no.2
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    • pp.72-76
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    • 2011
  • Antimicrobial activities of the Acidic Liquid originated from Carbonized Rice Hull(ALCRH) and the paper treated with ALCRH were investigated to apply ALCRH to functional paper products as a natural antimicrobial agent. ALCRH showed antimicrobial activity for bacteria and yeast, with higher performance for bacteria than for yeast. Antimicrobial activity was not developed on paper coated with ALCRH by bar coater probably due to the evaporation of antimicrobial compounds of ALCRH from the paper surface with time. Saturation of paper with ALCRH was essential to develop antimicrobial activity on the paper. Dipping paper in ALCRH solution was recommended as one of the effective ways to make antimicrobial paper.

Antimicrobial and Cytotoxic Activities of Some Malaysian Flowering Plants

  • Jasril, Jasril;Mohamed, S.M.;Mackeen, M.M.;Lajis, N.H.;Rahman, A.A.;Ali, A.M.
    • Natural Product Sciences
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    • v.5 no.4
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    • pp.172-176
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    • 1999
  • A total of 43 extracts from 21 species of Malaysian flowering plants were screened for antimicrobial and cytotoxic activities. Antimicrobial activity was tested against fungi, including yeast and candida, as well as Gram-positive and Gram-negative bacteria, and cytotoxicity was assayed using the CEM-SS and HT-29 cell lines. The methanol extracts of the roots and stems of Plumeria acutifolia showed the highest antimicrobial activity, i.e. against Saccharomyces cerevisiae and Candida lipolytica, and mostly moderate activity against the other microbes such as Aspergillus ochraceous and Saccharomyces lipolytica. Phyllanthus emblica also showed moderate activity especially that of the methanol extracts. The methanol extract of the roots of Plumeria acutifolia showed the strongest cytotoxic activity $(CD_{50}=\;3\;{\mu}g/ml)$. Most of Zingiberaceae species gave negative results for antimicrobial activity and showed low cytotoxic activity.

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Antimicrobial Activity and Bactericidal Activity of Caesalpinia sappan L. Extract (소목 추출물의 항균력 및 살균소독력)

  • Lee, Jin-Young;Min, Kyung-Jin
    • Journal of Environmental Health Sciences
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    • v.37 no.2
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    • pp.133-140
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    • 2011
  • Antimicrobial activity and bactericidal activity of Caesalpinia sappan L. extracts were investigated against five food-borne pathogens, E. coli, S. aureus, S. typhimurium, B. cereus and L. monocytogenes. Methanol extract of Caesalpinia sappan L. revealed antimicrobial activities against five pathogens. In particular, by paper disc diffusion the highest activity was shown against L. monocytogenes. Antimicrobial activities of methanol extracts showed the most potent activities, but hexane fraction had no activity. Fractions of ethyl acetate and butanol turned out to have higher antimicrobial activities against Gram(+) bacteria than Gram(-) bacteria. The minimum inhibitory concentration against five food-borne pathogens was 1.563 mg/ml on Gram(+) bacteria and 3.125 mg/ml on Gram(-) bacteria. The result of antimicrobial activity in a shaking flask method showed that bacterial growth rate fell by more than 99.999% at 3.125 mg/ml of methanol extract. The highest rate of viable reduction (99.998%) was shown at 0.781 mg/ml of methanol extract against L. monocytogenes. After five minutes of reaction between test strains and methanol extracts, the growth rates of five kinds of bacteria were reduced by more than 99.999% at a concentration of 100 mg/ml. Therefore, it is suggested that methanol extracts of Caesalpinia sappan L. can be developed as a natural sanitizer or disinfectant.