• Title, Summary, Keyword: antigenotoxicity

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Antigenotoxicity of Quercetin and Its Glycosides Against Benzo(a)pyrene-induced Genotoxicity (퀘르세틴 및 퀘르세틴 배당체들의 벤조피렌에 대한 유전독성억제효과)

  • Kim, Jeong-Han;Heo, Moon-Young
    • YAKHAK HOEJI
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    • v.42 no.4
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    • pp.414-421
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    • 1998
  • In order to compare the suppressive effect of quercetin and its glycosides, such as quercitrin (quercetin-3-rhamnoside), isoquercitrin (quercetin-3-glucoside), hyperin (querceti n-3-galactoside)and rutin (quercetin-3-rhamnosyl glucoside), on the genotocicity by benzo(a)pyrene(B(a)P), in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes and in vivo micronucleus test using mouse peripheral blood were performed. B(a)P-induced SCEs in vitro were slightly decreased by the simultaneous treatment of quercetin and its glycosides, although there was no significant decrease. On the other hand, MNU induced micronucleated reticulocytes(MNRL7s) in vivo were significantly decreased with a dose-dependent manner in all compounds tested. However, there were no differences between quercetin aglycone and glycosides in the suppressive effects under experimental condition of this study. To elucidate, the action mechanism of quercetin aglycone and its glycosides against B(a)P-induced genotoxicity, the assay of DNA binding with B(a)P was studied. Quercetin aglycone and its glycosides inhibited B(a)P metabolism in the presence of S-9 mix and decreased the B(a)P/DNA binding in the calf thymus DNA with S-9 mix. These results suggest that antigenotoxicity of quercetin antiglycosides on B(a)P-induced genotoxicity is due to decrease of DNA binding with B(a)P through the inhibition of metabolism with B(a)P in the calf thymus DNA. Therefore, quercetin and its glycosides may act as an antigenotoxicity agent and may be useful as a chemopreventive agent of polycyclic aromaic hydrocarbons like B(a)P.

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The Biochemical Properties of Korean radish (Raphanus sativus L.) and Its Seed Protein as a Natural Preservative : The Influences on Antigenotoxicity and Cytotoxicity (천연보존제로서 한국산 무 및 종자 단백질의 생화학적 특성 : 항유전독성 및 세포독성에 미치는 영향)

  • Choi Yoon-Hyeok;Hwang Cher-Won
    • Journal of Life Science
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    • v.15 no.4
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    • pp.561-565
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    • 2005
  • In this study, we report antigenotoxicity and cytotoxicity of Korean Radish extract (RJ) and its seed protein (RSP) to non-tumoral 3T3 cell line. In the case of antigenotoxicity, each cell line was treated with $10{\mu}l\;of\;100{\mu}g/ml$ N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) before adding $10{\mu}l$of 10mg/ml RJ and 1mg/ml RSP to the cell. Both RJ and RS were shown $30\%\;and\;43\%$ of antigenotoxicity respectively. As a result of quantitative analysis for lactose dehydrogenase (LDH), no cytotoxic activity against 3T3 cells was detected when the cells were treated with various concentrations of RJ and RSP, RSP showed $85\%$ of antimicrobial activity against cosmetic sample (C1) assumed as contaminated by bacteria. RSP and RJ showed $79\%\;and\;76\%$ of antimicrobial activities repectively on another cosmetic sample (C4, contaminated by fungi) were treated with 10mg/ml RJ and 1 mg/ml RSP

A Study on Antimutagenic Acitivities of the Extracts from Dianthi Herba (구맥(瞿麥)의 항돌연변이(抗突然變異) 활성(活性)에 관한 연구(硏究))

  • Seo Un-Kyo;Jeong Ji-Cheon
    • Herbal Formula Science
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    • v.11 no.2
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    • pp.197-212
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    • 2003
  • Antigenotoxicity test (SOS chromotest) and antimutagenecity test (Ames test) were carried out using water-soluble and methanolic extracts from Dianthi Herba. Antigenotoxic activity of methanolic extract against mutagens both MNNG and NQO was much more effective than that of water-soluble one. When the extract was added to the certain concentration $(100\;{\mu}l/tube)$, antigenotoxic activities against both mutagens were enhanced. Against the mutagen MNNG with Ames test, antimutagenic activity of the methanolic extract was better than that of water-soluble one. The 74.6% of inhibition ratio for revertant forming CFU/plate was shown at $300\;{\mu}l/plate$ of the methanolic extract.

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Antigenotoxicity of Quercetin and its Glycosides (Quercetin 및 Quercetin 배당체들의 유전독성억제효과)

  • 허문영;김정한
    • Journal of Food Hygiene and Safety
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    • v.11 no.2
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    • pp.115-121
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    • 1996
  • In order to compare the suppressive effect of quercetin and several its glycosides, such as quercitrin (quercetin-3-rhamnoside), isoquercitrin (quercetin-3-glucoside), hyperin (quercetin-3-galactoside) and tutin (quercetin-3-rhamnosyl glucoside), on the genotoxicity by N-methyl-N-nitrosourea(MNU), in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes and in vivo micronucleus test using mouse peripheral blood were performed. MNU-induced SCEs in vitro were not decreased by the simultaneous treatment of test compounds. Among them, quercetin and hyperin showed significant suppressive effects at high dose(10-5M). On the other hand, MNU-induced micronucleated reticulocytes(MNRETS) in vivo were significantly decreased with good dose-dependent manner in all compound tested. However, there were not significant differences between quercetin aglycone and its glycosides in the suppressive aglycone and its glycosides may act as an antigenotoxic agent in vivo and may be useful as a chemopreventive agent of alkylating agent.

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Antigenotoxicity and Action Mechanism of Quercetin and its Glycosides against Oxidative DNA Damage (Oxidative DNA 손상에 대한 Quercetin 및 그 배당체들의 유전독성억제효과와 작용기전)

  • 김수희;허문영
    • Environmental Mutagens and Carcinogens
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    • v.19 no.2
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    • pp.116-121
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    • 1999
  • Quercetin and its glycosides showed a strong free radical scavenging effect to DPPH radical generation. However, there were not big differences between quercetin aglycone and glycosides under experimental condition of this study. On the other hand, quercetin had pro-oxidant effect in bleomycin-dependent DNA assay. Quercetin aglycone and its glycosides, quercitrin inhibited $H_2$$O_2$- induced DNA damage in CHL cells. They also have an anticlastogenicity toward DNA breakage agent by radical generation like bleomycin. These results indicate that quercetin aglycone and its glycosides are capable of protecting the free radical generation induced by reactive oxygen species like $H_2$$O_2$. The mechanism of inhibition in hydrogen peroxide-induced genotoxicity may be due to their free radical scavenging properties. Therefore, quercetin aglycone and its glycosides may be useful chemopreventive agents by protecting of free radical generation which are involved in carcinogenesis and aging. However, quercetin and its glycosides must also carefully examined for pro-oxidant properties before being proposed for use in vivo.

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A Study on Antimutagenic acitivities of from Puerariae Flos Extracts (갈화(葛花)의 항돌연변이(抗突然變異) 활성(活性)에 관한 연구(硏究))

  • Jeong Young-Jae;Kim Mi-Rang;Jeong Ji-Cheon;Seo Woon-Gyo
    • The Journal of Internal Korean Medicine
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    • v.25 no.1
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    • pp.106-118
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    • 2004
  • Antigenotoxicity test (SOS chromotest), antimutagenecity test (Ames test), and antioxidant test (NBT method and xanthine-xanthine oxidase method) were carried out using water-soluble and methanolic extracts from Puerariae Flos. Against the mutagens MNNG and NQO, antigenotoxic activity of methanolic extracts were much more effective than that of water-soluble ones. When the methanolic extract was added to the certain concentration $(100{\mu}{\ell}/tube)$, antigenotoxic acivity against the mutagen MNNG was enhanced. Contrary to the water-soluble extract, the methanolic extract showed high antigenotoxicity against the mutagen NQO with increment of the extract. Against the mutagen MNNG with Ames test, antimutagenic activity of the methanolic extract at $300{\mu}{\ell}/tube$ was 96% as an inhibition ratio of revertant forming CFU/plate. The antioxidant activity of water-soluble extract was comparatively higher than that of the methanolic one.

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In Vitro Inhibition of 4-Nitroquinoline-1-Oxide Genotoxicity by Probiotic Lactobacillus rhamnosus IMC501

  • Bocci, Alessandro;Sebastiani, Bartolomeo;Trotta, Francesca;Federici, Ermanno;Cenci, Giovanni
    • Journal of Microbiology and Biotechnology
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    • v.25 no.10
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    • pp.1680-1686
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    • 2015
  • Inhibition of 4-nitroquinoline-1-oxide (4-NQO) genotoxicity by a probiotic strain of Lactobacillus rhamnosus (IMC501) was assessed by the prokaryotic short-term bioassay SOSChromotest, using Escherichia coli PQ37 as the target organism. Results showed the ability of strain IMC501 to rapidly and markedly counteract, in vitro, the DNA damage originated by the considered genotoxin. The inhibition was associated with a spectroscopic hypsochromic shift of the original 4-NQO profile and progressive absorbance increase of a new peak. IR-Raman and GC-MS analyses confirmed the disappearance of 4-NQO after contact with the microorganism, showing also the absence of any genotoxic molecule potentially available for metabolic activation (i.e., 4-hydroxyaminoquinoline-1-oxide and 4-nitrosoquinoline-1-oxide). Furthermore, we have shown the presence of the phenyl-quinoline and its isomers as major non-genotoxic conversion products, which led to the hypothesis of a possible pattern of molecular transformation. These findings increase knowledge on lactobacilli physiology and contribute to the further consideration of antigenotoxicity as a nonconventional functional property of particular probiotic strains.

Antigenotoxicity of Vegetable and Fruit Extracts

  • Chan Heo;Lee, Seung-Chul;Kim, Hyun-Pyo;Heo, Moon-Young
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.151-151
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    • 2003
  • The ethanol extracts of mixed vegetables (Bioactive V, BV), mixed fruits (Bioactive F, BF) and their liquid formulation (Chungpae Plus(R)) were evaluated for antioxidative and antigenotoxic activity. They were shown to possess the significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and were revealed to show the inhibitory effect of lipid peroxidation as measured by malondialdehyde (MDA) formation.(omitted)

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The Antigenotoxic Effects of Korean Native Fermented Food, Baechu Kimchi Using Comet Assay (Comet Assay를 이용한 전통발효식품인 배추김치의 항유전 독성효과)

  • 지승택;박종흠;현창기;신현길
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.2
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    • pp.316-321
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    • 2000
  • This study carried out to eluciate the cancer chemoprevention of Korean native fermented food, baechu kimchi using Comet assay (in other words, single cell microgel electrophoresis). For this purpose, baechu kimchi was fractionated by water, n-hexane, chloroform and ethyl acetate. 5 strains of dominant fermented bacteria were isolated from baechu kimchi. The water fraction, n-hexane fraction, chloroform fraction, ethyl acetate fraction and water insoluble fractions showed no antigenotoxicitie in non-tumoral normal 3T3 cells. Among 5 bacteria isolates from baechu kimchi, two isolates bacteria 1 and 2 strongly inhibited genotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in non-tumoral normal 3T3 cells (p<0.05). Bacteria 3, 4 and 5 were also not antigenotoxic.

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