• Title, Summary, Keyword: aniline

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Plasmid-Mediated Aniline Assimilation by Pseudomonas sp. B10

  • El-Deeb, Bahig A.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.1
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    • pp.79-84
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    • 2001
  • An aniline-utilizing microorganism identified as a species of Pseudomonas was isolated from soil contaminated highly with aniline and urea-herbicide. This strain was able to utilize aniline as the sole source of carbon and energy, and was shown to harbor a single large plasmid mediating the aniline assimilation. Subsequent plasmid-curing of this bacterium resulted in the abolishment of the aniline utilizing phenotype and the loss of catechol-C2,3O-oxygenase. The reestablishment of the plasmid, denoted pB10, in cured Pseudomonas sp. via filter surface mating, resulted in restoration of the aniline assimilation abilities and enzyme activity.

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Synthetic Studies on the Nucleophilic Addition of Cysteine and Thiophenol to ${\alpha}$,N-Diphenylnitrone Derivatives (${\alpha}$,N-Diphenylnitrone 유도체에 대한 Cysteine 및 Thiophenol의 친핵성 첨가물에 관한 연구)

  • Tae-Rin Kim;Sang-Yong Pyun;Man-So Han;Kwang-Il Lee
    • Journal of the Korean Chemical Society
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    • v.35 no.3
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    • pp.258-261
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    • 1991
  • Four thiazolidines and following five new compounds were prepared by the addition reaction of cysteine and thiophenol to ${\alpha}$,N-diphenylnitrones, respectively ; ${\alpha}$,thiophenoxy-benzylidene aniline ;${\alpha}$,thiophenoxy-p-hydroxybenzylidene aniline ; ${\alpha}$,thiophenoxy-p-chlorobenzylidene aniline ;${\alpha}$,thiophenoxy-p-methoxybenzylidene aniline ; ${\alpha}$,thiophenoxy-p-nitrobenzylidene aniline. The structure of these compounds were confirmed by the elemental analysis, UV-, IR-and NMR-spectra.

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Isolation and Characterization of Stenotrophomonas maltophilia Strains Capable of Degrading Aniline. (아닐린 분해 세균인 Stenotrophomonas maltophilia의 분리 및 특성)

  • 김현주;김진철;김흥태;최경자;최도일;김홍기;조광연
    • Microbiology and Biotechnology Letters
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    • v.28 no.4
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    • pp.202-208
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    • 2000
  • Four bacteria capable of using aniline as a sole source of carbon and energy we4e isolated from river waters. Among them, two strains were identified as Stenotrophomonas maltophilia based on their physiological and biochemical characteristics and 16SrRNA gene sequence and the others as delftia acidovorans. The four strains were able to grow on the mineral salt media containing aniline at concentrations up to 6,000 $\mu\textrm{g}$/ml. Since aniline degradation by S. maltophilia has not been reported so far, the two strains A-s and 51-4 were selected for further studies. They completely utilized aniline in a mineral salt medium containing 300 $\mu\textrm{g}$/ml of aniline as a sole carbon and energy source within 24 hours. Optimum pH and temperature for aniline degradation and cell growth of both strains were 7.0 and $35^{\circ}C$, respectively. In addition, they effectively degraded aniline is waste, underground and river waters containing 300 $\mu\textrm{g}$/ml of aniline. This is the first report of aniline degradation by S. maltophilia strains.

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Nutritional Biochemical Study on the Pollen Load -Effect of Azalea Pollen on the Aniline-induced Hepatotoxicity- (화분의 영양생화학적 연구 -Aniline이 유도한 간 독성에 미치는 진달래 화분의 영향-)

  • Kwon, Chong-Suk;Cho, Soo-Yeul;Chung, Hyeung-Jae;Park, Jong-Min;Huh, Keun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.3
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    • pp.239-246
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    • 1989
  • Preventive effect of azalea pollen extracts against aniline-induced hepatic toxicity in mice was investigated in this experiment. When the biochemical and histological changes were measured, preventive effect was more striking by treatment with water extract. After treatment with azalea pollen extracts, hepatic microsomal aniline hydroxylase activity increased as compared to control. Whereas, aniline level in serum and liver significantly decreased. The Vmax value without affecting Km value increased by the water extract treatment, the results obtained suggest that the characteristics of increase in the aniline hydroxylase activity may include induction of enzyme proteins. These data indicate that the observed preventive effects of azalea pollen extracts against hepatotoxicity is due to the induction of aniline metabolizing enzyme.

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Characterization of an Aniline-degrading Bacterium, Delftia sp. JK-2 Isolated from Activated Sludge of Municipal Sewage Treatment Plant (도시폐수처리장의 활성슬러지에서 분리한 Aniline 분해세균 Delftia sp. JK-2의 특성연구)

  • 조윤석;강형일;장효원;오계현
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.79-83
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    • 2000
  • Activated sludge samples were collected from a municipal sewage treatment plant and used for enrichment of microbial consortia with aniline as the sole carbon and nitrogen source. Threc aniline-degrading bacteria were obtained lrom microbial consortia and an isolate which has excellent aniline degradability was selected for this study. The isolate was Gram-negative, and identified and designated as Delfha sp. JK-2 on the basis of various physiological and biochemical tests. 10 mM aniline was completely degraded within 24 hours after inoculation of the culture. Ammonium ion was liberated in the medium transiently during the incubation and disappeared when aniline was completely degraded. Addition of glucose as a supplementary source to aniline minimal media showed significant decrease in aniline degradat~on rate for the strain Effective degradation of aniline was achieved by the addition of 0.5% nitrate as a nitrogen source, and resulted in approximately 80% higher aniline degradation compared to the absence of nitrate. Phylogenetic analysis based on 16s [DNA sequence revealed that the strain was closely related to De@ia acidovorans, with 96% overall similarity. The 16s [DNA sequence of JK-2 was also found to be closely related to those of six other clonal types, including Acidovoru, Aquaspirillum. Xylophilus, Variovorm, and Rhodofernr.

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Comparative Analysis of Aniline Dioxygenase Genes from Aniline Degrading Bacteria, Burkholderia sp. HY1 and Delftia sp. HY99. (Aniline 분해균주 Burkholderia sp. HY1과 Delftia sp. HY99에서 유래된 Aniline Dioxygenases 유전자의 비교 분석)

  • Kahng, Hyung-Yeel;Oh, Kye-Heon
    • Microbiology and Biotechnology Letters
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    • v.35 no.2
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    • pp.104-111
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    • 2007
  • In this study, aniline dioxygenase genes responsible for initial catabolism of aniline in Burkholderia sp. HY1 and Delftia sp. HY99 were cloned and the amino acid sequences were comparatively analyzed, which already have been reported as bacteria utilizing aniline as a sole source of carbon and nitrogen, B. sp. HY1 was found to have at least a plasmid, and the plasmld-cured strain, B. sp. HY1-PC obtained using mitomycin C was tested with wild type strain to investigate whether the former maintained the degradability for aniline. This proved that the aniline oxygenase gene from B. sp. HY1 was located in chromosomal DNA, not in plasmid DNA. Aniline dioxygenase small subunits from B. sp. HY1 and D. sp. HY99 were found, based on 146 amino acids, to share 79% similarity. Notably, ado2 genes from B. sp. HY1 and D. sp. HY99 which were found to be terminal dioxygenase of aniline dioxygenase small subunit showed 99% similarity in the deduced amino acid sequences with tdnA2 of Frateuria sp. ANA-18 and danA2 of D. sp. AN3, respectively. Besides, enzyme assay and amino acid sequence analysis of catechol dioxygenase supported the previous report that B. sp. HY1 might occupy ortho-cleavage pathway using catechol 1,2-dioxygenase, while D. sp. HY99 might occupy catechol 2,3-dioxygenase for meta-cleavage pathway.

Nutritional Biochemical Study on the Pollen Load. -Effect of Azalea(Rhododendron mucronulatum)Pollen on the Hepatic Microsmal Aniline Hydroxylase Activity- (화분의 영양생화학적 연구 -진달래 화분(Rhododendron mucronulatum)이 간 Aniline Hydroxylase 활성에 미치는 영향-)

  • Kwon, Chong-Suk;Cho, Soo-Yeul;Park, Jong-Min;Huh, Keun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.1
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    • pp.93-100
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    • 1989
  • The effect of each azalea(Rhodoendron mucronulatum) pollen extract on phase I enzyme(aniline hydroxylase)was studied in this experiment. Serum aminotransferases were not changed in mice injected each azalea pollen extract, respectively compared to control group. The hepatic micros mal aniline hydroxylase activities in the presence of each azalea pollen extract were not affected in vitro. After treatment with azalea pollen water extract, hepatic microsomal aniline hydroxylase activity was increased with dose-dependent manner as compared to control group. The increment of hepatic microsmal aniline hydroxylase activity was more powerful by the treatment of water extract. As mice received aniline after pollen butanol and water extract-pretreatment once a day for 5 days, the blood and liver levels of aniline were decreased significantly.

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Treatment of Aniline-contaminated Wastewater using Oxidation Reagent (산화제를 이용한 아닐린 폐수처리)

  • 김광렬;신진환
    • Journal of environmental and Sanitary engineering
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    • v.12 no.2
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    • pp.51-57
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    • 1997
  • This work carried out the removal of aniline by wet oxidation in aqueous solutions like a industrial wastewater using Ozone, UV, and Ozone-UV . The main features of this experiment are as follows: the aniline was decomposed by OH and HO$_{2}$ radicals which produced from the reaction of water with UV and Ozone, when the Ozorie and Ozone-UV used the aniline was decomposed completely. The decomposition of aniline was very fast reaction and the reaction times were within 10min. and 20min. in case of for Ozone Ozone-UV respectively. Assumed simplified reaction mechanism from the aniline oxidation model, and the we are calculated the theoretical reaction rate constants by computer simulation, and then compared with experimental data. We suggest that this simulation program is applicable to estimate of the aniline decaying concentration and removal efficiency of aniline - contaminated wastewater.

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Isolation and Characterization of Aniline-Degrading Bacteria

  • Kahng, Hyung-Yeel;Kim, Seung-Il;Woo, Mi-Jeong;Park, Yong-Keun;Lee, Yung-Nok
    • Korean Journal of Microbiology
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    • v.30 no.3
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    • pp.199-206
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    • 1992
  • Six isolated strains degrading aniline were selected, identified and designated as pseudomonas putida K6, Pseudomonas acidovorans K82, Achromobacter gr. D. V. K24, Achromobacter xylosocidans K4, Moraxella sp. K21 and Moraxella sp. K22. All of them degraded 1000 ppm aniline completely within 30 to 36 hours. Most of these strains are resistant to antibiotics more than one, but Moraxella sp. has not any antibiotic marker tested. Most strains except for P. acidovorans K82 were shown to have resistance to the heavy metal ions such as Ni, Cu, Li, Ba, Co, etc. but not to Hg to which only P. putida K6 was resistant. M. sp. K21 was capable of degrading aniline to a maximum concentration of 2500 ppm without any repression. The incubation of the cell in limited pH ranges (4-8) had no great effect on aniline degradation. The addition of bactopeptone to the minimal media promoted the speed of aniline degradation, but the addition of glucose rather repressed the rate of aniline degradation. Through enzyme assay, A. gr. D. V. K 24 was shown to degrade aniline through artho-pathway and formed .betha.-ketoadipate as intermediate metabolite.

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Highly Luminescent Aniline and TiO2 Composite: The Effect of Weight Ratio of Aniline and TiO2

  • Kim, Byoung-Ju;Park, Eun-Hye;Kang, Kwang-Sun
    • Current Photovoltaic Research
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    • v.4 no.1
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    • pp.8-11
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    • 2016
  • Strong deep ultraviolet emitting aniline and $TiO_2$ composite has been synthesized via hydrolysis and condensation reactions of titaniumisopropoxide ($Ti(OPr)_4$), aniline, and acetic anhydride. Three different weight ratios of aniline and $Ti(OPr)_4$ including 3:1 ($TiO_2An-A$), 2:1 ($TiO_2An-B$), and 1:1 ($TiO_2An-C$) were synthesized and characterized their optical properties. The FTIR spectra of the $TiO_2An-A$, -B, and -C showed the absorption intensities of the benzene ring stretching and bending vibrations, and benzene ring -CH stretching, bending, and deformation vibrations increased with the increase of the amount of aniline. The UV-visible absorption spectra showed that the UV region absorption was slightly increased with the increase of the amount of aniline. The photoluminescence (PL) intensities were exponentially increased with the increase the excitation wavelength from 307 to 317 nm, steadily increased from 300 to 313 nm and slowly increased from 302 to 308 nm for $TiO_2An-A$, -B, and -C, respectively and decreased thereafter. Therefore, the PL intensity is strongly dependent on the weight ratio of $Ti(OPr)_4$ and aniline.