• Title, Summary, Keyword: aldose reductase

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Inhibitory Effects of Ginseng Components on Rat Lens Aldose Reductase Activities (인삼성분의 Rat Lens Aldose Reductase 활성에 대한 억제효과)

  • Kim, Hack-Seang;Park, Ung-Yang;Seong, Yeon-Hee
    • Journal of Ginseng Research
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    • v.20 no.1
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    • pp.106-110
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    • 1996
  • The present study was undertaken to elucidate the effects of ginseng components on rat lens aldose reductase activity. Ginseng total saponin (GTS) exhibited inhibitory activities on rat lens aldose reductase in a dose-dependent manner. Among ginsenosides, Rf and Rgl showed potent inhibitory activities on rat lens aldose reductase. Lipid soluble fraction also inhibited rat lens aldose reductase activities. These data suggest that ginseng components inhibit rat lens aldose reductase activity in vitro.

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Rat Lens Aldose Reductase inhibitory of Taraxacum mongolicum and two Cirsium species (민들레와 두종의 엉겅퀴의 Rat lens aldose reductase 억제활성)

  • Jung, Mee-Jung;Heo, Seong-Il;Wang, Myeong-Hyeon
    • Journal of Applied Biological Chemistry
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    • v.51 no.6
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    • pp.302-306
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    • 2008
  • To evaluated active principles for diabetic compolications from the Taraxacum mongolicum (T. mongolicum), Cirsium japonicum (C japonicum), and Cirsium setidens (C. setidens) and its constituents, as well as silymarin tested for their effect on rat lens aldose reductase. As a result, the MeOH extract of T. mongolicum ($IC_{50}\;8.71\;{\mu}g/mL$) have strong lens aldose reductase inhibition abilities. Also, Aldose reductase inhibitory showed an isolated luteolin ($IC_{50}\;20\;{\mu}M$) from the T. mongolicum and sliymarin ($IC_{50}\;13\;{\mu}M$).

Inhibition of Lens Aldose Reductase by Medicinal Plants (수종 생약에 의한 Lens Aldose Reductase의 억제)

  • Jeong, Do-Rae;Park, Woong-Yang;Lee, Seung-Ho;Lee, Kyong-Soon;Ro, Jai-Seup
    • Korean Journal of Pharmacognosy
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    • v.24 no.1
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    • pp.58-62
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    • 1993
  • To search for inhibitory activities for lens aldose reductase, twenty two medicinal plants were tested by the method of bovine lens aldose reductase inhibition test. In this test Rubia akane(68%), Artemisia selengensis(61%), Lespedeza cuneata (58%), Ligustrum lucidum(58%), Viola patrinii(58%) extracts were shown to have aldose reductase inhibitory activity. From the radix of Rubia akane, $2-methyl-1,\;3,\;6-trihydroxy-9,\;10-anthraquinone-3-O-(6'-O-acetyl)-{\alpha}-rhamnosyl-(1{\rightarrow}2)-{\beta}-D-glucoside$ was isolated and the $IC_{50}$ of this compound in the aldose reductase inhibition test was about $1.2{\times}10^{-6}M$.

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Screening for Aldose Reductase Inhibitory Activity of Extracts of the Marine Plants from Korea (한국산 해산식물의 추출물로부터 Aldose Reductase 억제활성의 검색)

  • Lee, Yeon-Sil;Lee, Sang-Hyun;Kim, Bak-Kwang;Shin, Kuk-Hyun
    • ALGAE
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    • v.19 no.4
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    • pp.349-352
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    • 2004
  • We examined MeOH extracts of the marine plants from Korea for their inhibitory activities on rat lens aldose reductase in vitro. Among the MeOH extracts tested, the extracts of Enteromorpha prolifera, Ecklonia cava, Pelvetia siliquosa and Salicornia herbacea exhibited a rat lens aldose reductase inhibition (IC$_{50}$: 3.04, 8.84, 11.42 and 4.99 $\mu$g-ml$^{-1}$, respectively) in vitro.

Isoflavonoids from the Rhizomes of Belamcanda chinensis and Their Effects on Aldose Reductase and Sorbitol Accumulation in Streptozotocin Induced Diabetic Rat Tissues

  • Jung, Sang-Hoon;Lee, Yeon-Sil;Lee, Sanghyun;Lim, Soon-Sung;Kim, Yeong-Shik;Shin, Kuk-Hyun
    • Archives of Pharmacal Research
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    • v.25 no.3
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    • pp.306-312
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    • 2002
  • Aldose reductase, the key enzyme of the polyol pathway, is known to play important roles in the diabetic complication. The inhibitors of aldose reductase, therefore, would be potential agents for the prevention of diabetic complications. To evaluate active principles for the inhibition of aldose reductase from the rhizomes of Belamcanda chinensis, twelve phenolic compounds were isolated and tested for their effects on rat lens aldose reductase. As a result, isoflavones such as tectorigenin, irigenin and their glucosides were found to show a strong aldose reductase inhibition. Tectoridin and tectorigenin, exhibited the highest aldose reductase inhibitory potency, their $IC_{50}$ values, being $1.08{\times}10^{-6}{\;}M{\;}and{\;}1.12{\times}10^{-6}{\;}M$, respectively, for DL-glyceraldehyde as a substrate. Both compounds, when administered orally at 100 mg/kg for 10 consecutive days to streptozotocin-induced diabetic rats, caused a significant inhibition of sorbitol accumulation in the tissues such as lens, sciatic nerves and red blood cells. Tectorigenin showed a stronger inhibitory activity than tectoridin. From these results, it is suggested that tectorigenin is attributed to be a promising compound for the prevention and/or treatment of diabetic complications.

Isolation and Structural Determination of Aldose Reductase Inhibitor from Korean Fermented Soybean Paste

  • Choi, Sung-Won;Yang, Jae-Sung;Jung, Eun-A;Choi, Hak-Jong;Lee, Han-Seung;Shin, Chul-Soo;Kim, Dong-Seob;Hur, Nam-Yun;Baik, Moo-Yeol
    • Food Science and Biotechnology
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    • v.14 no.3
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    • pp.344-349
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    • 2005
  • Aldose reductase catalyzes the conversion of glucose into sorbitol. Inhibiting this enzyme in diabetes mellitus can delay or prevent pathogenic process. Aldose reductase inhibitor was screened from Korean fermented soybean pastes (Doen-jang) and purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. Aldose reductase inhibitor was identified as genistein with molecular weight of 270 Da and molecular formula of $C_{15}H_{10}O_5$ based on UV spectrometry, $^1H$ and $^{13}C\;NMRs$, and mass spectrometry. Genistein inhibited aldose reductase of pig lens with $IC_{50}$ level of $20\;{\mu}M$. Because genistein was effective against aldose reductase of animal source, it may be a potential therapeutic agent for diabetic complications.

Esculetin, a Coumarin Derivative, Inhibits Aldose Reductase Activity in vitro and Cataractogenesis in Galactose-Fed Rats

  • Kim, Chan-Sik;Kim, Junghyun;Lee, Yun Mi;Sohn, Eunjin;Kim, Jin Sook
    • Biomolecules & Therapeutics
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    • v.24 no.2
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    • pp.178-183
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    • 2016
  • Naturally occurring coumarin compounds have received substantial attention due to their pharmaceutical effects. Esculetin is a coumarin derivative and a polyphenol compound that is used in a variety of therapeutic and pharmacological strategies. However, its effect on aldose reductase activity remains poorly understood. In this study, the potential beneficial effects of esculetin on lenticular aldose reductase were investigated in galactose-fed (GAL) rats, an animal model of sugar cataracts. Cataracts were induced in Sprague-Dawley (SD) rats via a 50% galactose diet for 2 weeks, and groups of GAL rats were orally treated with esculetin (10 or 50 mg/kg body weight). In vehicle-treated GAL rats, lens opacification was observed, and swelling and membrane rupture of the lens fiber cells were increased. Additionally, aldose reductase was highly expressed in the lens epithelium and superficial cortical fibers during cataract development in the GAL rats. Esculetin reduced rat lens aldose reductase (RLAR) activity in vitro, and esculetin treatment significantly inhibited lens opacity, as well as morphological alterations, such as swelling, vacuolation and liquefaction of lens fibers, via the inhibition of aldose reductase in the GAL rats. These results indicate that esculetin is a useful treatment for galactose-induced cataracts.

Aldose Reductase Inhibitory Alkaloids from Corydalis ternata

  • Seo, Wonse;Jung, Sang Hoon;Shim, Sang Hee
    • Natural Product Sciences
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    • v.22 no.2
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    • pp.102-106
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    • 2016
  • A methanolic extract of Corydalis ternata having aldose reductase inhibitory activity was examined as a possible aldose reductase (ALR2) inhibitor, a key enzyme involved in diabetic complications. Seven alkaloids, tetrahydrocoptisine (1), corydaline (2), tetrahydropalmatine (3), isocorybulbine (4), corybulbine (5), dehydrocorydaline (6), and N-methyltetrahydroberbinium (7) were isolated from $CHCl_3$ fraction of C. ternata methanol extract. Among them, compounds 1, 5, and 7 exhibited $5.04{\pm}1.97%$, $5.00{\pm}1.26%$, and $1.80{\pm}2.33%$ inhibitions, respectively at $40{\mu}M$. The activities of the single compounds were not comparable to that of the whole extract, suggesting that the whole combination of each single compound was responsible for the activity of the extract as shown in many cases of natural medicines. Even though this is the second report on aldose reductase inhibition activity of C. ternata, recombinant human aldose reductase was employed in this study unlike in the previous report. Furthermore, the aldose reductase inhibitory activities of isocorybulbine, corybulbine, and N-methyltetrahydroberbinium, to the best of our knowledge, were evaluated for the first time in this study. These results suggest a use of the extract of C. ternata for ameliorating diabetic complications.

Hyperin, An Aldose Reductase Inhibitor from Acanthopanax senticosus Leaves

  • Lee, Sang-Hyun;Jung, Sang-Hoon;Lee, Yeon-Sil;Shin, Kuk-Hyun
    • Natural Product Sciences
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    • v.9 no.1
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    • pp.4-6
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    • 2003
  • Hyperin from the leaves of Acanthopanax senticosus was tested for its effect on rat lens aldose reductase and demonstrated to exhibit a significant inhibition of rat lens aldose reductase activity with $IC_{50}$ value of $2.63\;{\mu}M$.

Coumarins from Angelica gigas Roots having Rat Lens Aldose Reductase Activity

  • Lee, Sang-Hyun;Jung, Sang-Hoon;Lee, Yeon-Sil;Shin, Kuk-Hyun
    • Biomolecules & Therapeutics
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    • v.10 no.2
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    • pp.85-88
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    • 2002
  • Systematic fractionation of Angelica gigas roots led to the isolation of linear coumarins such as decursinol angelate, decursin and nodakenin. These compounds were tested for their effects on rat lens aldose reductase. Nodakenin was demonstrated to exhibit significant inhibition of rat lens aldose reductase activity with $IC_{50}$ value of $7.33\Mu\textrm{M}$.