• Title, Summary, Keyword: acute toxicity

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Acute Toxicity Study on Yeoldahanso-tang in Mice (Mouse(ICR)에 있어서 시험물질 열다한소탕(熱多寒少湯) 추출액의 급성독성 시험)

  • Ma, Jin-Yeul;Huang, Dae-Sun;Lee, Si-Woo;Kim, Jong-Yeol;Shin, Hyeun-Kyoo
    • Journal of Sasang Constitutional Medicine
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    • v.21 no.1
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    • pp.247-253
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    • 2009
  • 1. Objectives This study was designed to andyze the acute toxicity and safety of Yeoldahanso-tang 2. Methods We investigated the acute toxicity for water-extracted Yeoldahanso-tang. 25 male and 25 female mice were observed for 14 days after one day oral administration of Yeoldahanso-tang at the respective doses of 0(control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results We observed survival rates, general toxicity, change of body weight and autopsy. 4. Conclusions The data confirmed that Yeoldahanso-tang is free from the toxicity and safety problems in oral route respectively. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). In conclusion, LD50 of Yeoldahanso-tang was over 5000 mg/kg and it is very safe to mice.

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Oral Toxicity of Crude Antifungal Compounds Produced by Lactobacillus Plantarum AF1 and Lactobacillus Plantarum HD1 (Lactobacillus plantarum AF1과 Lactobacillus plantarum HD1이 생성한 조항균 물질의 독성평가)

  • Chang, Hae-Choon;Koh, Sang-Bum;Lee, Jae-Joon
    • The Korean Journal of Community Living Science
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    • v.26 no.3
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    • pp.511-522
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    • 2015
  • This study investigates the acute and repeated-dose oral toxicity of crude antifungal compounds produced by Lactobacillus plantarum AF1 (Lb. plantarum AF1) and Lactobacillus plantarum HD1 (Lb. plantarum HD1) in male and female Sprague Dawley rats. In the acute toxicity study, crude antifungal compounds (500, 1,000, and 2,000 mg/kg) did not reduce mortality or produce significant changes in general behaviors or the gross appearance of external and internal organs. In the repeated-dose toxicity study, crude antifungal compounds were administered orally to rats at doses of 500, 1,000, and 2,000 mg/kg daily for 28 days. There were no test-article-related deaths, abnormal clinical signs, or body weight changes. In addition, there were no significant differences between groups treated with crude antifungal compounds and the control group in their organ weight, hematological and serum biochemical parameters, or any other factors. These results suggest that the acute or repeated-dose oral administration of crude antifungal compounds produced by Lb. plantarum AF1 plus Lb. plantarum HD1 is not toxic in male and female rats.

Experimental studies on the Acute Toxicity of Bos taurus.Ursus thibetanus extract solution(BU) for Herbal-acupuncture (우황(牛黃).웅담약침액(熊膽藥鍼液)(BU)의 급성독성(急性毒性)에 관한 실험적(實驗的) 연구(硏究))

  • Soh, Kyung-Sun;Jeong, Chang-Gil;Lee, Sang-Woon;Park, Pyung-Mo;Kim, Jae-Hyung;Kang, Dae-In;Jo, Hoo-Lee;Kim, Kwang-Ho
    • Journal of Pharmacopuncture
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    • v.4 no.3
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    • pp.69-83
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    • 2001
  • This experiment was carried out to study on the safety assessment of Bas tures . Ursus thibetanus extract solution(BU) for Herbal acupuncture. SD rats and ICR mice were used for acute toxicity test the results were summerized as follows; 1. In rats and mice, LD50 value could not be measured. 2. There were no abnormal finding in acute toxicity test treated BU for Herbal-acupuncture.

Acute Oral Toxicity Study of Kami-honghwa-tang (가미홍화탕의 단회 경구 투여 독성 연구)

  • Sung, Hyun-Jea;Moon, Geun-Ah;Ryoo, Choong-Ryeol;Yoon, Yoo-Sik
    • Korean Journal of Oriental Medicine
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    • v.9 no.2
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    • pp.95-105
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    • 2003
  • Kami-honghwa-tang(KH-19) is a prescription for reducing the side effect of radiotherapy. In this study, safety of KH-19 was evaluated by GLP guideline of Korea Food and Drug Administration. In acute oral toxicity study on rat, transient inhibition of weight increase was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and weight reduction was observed. However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

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Experimental studies on the Acute Toxicity of Bos taurus.Ursus thibetanus.Moschus extrct solution(BUM) for Herbal-acupuncture (우황(牛黃).태담(態膽).사향약침액(麝香藥鍼液)(BUM)의 급성독성(急性毒性)에 관한 실험적(實驗的) 연구(硏究))

  • Lee, Sang-Woon;Kang, Dae-In;Jeong, Chan-Gil;Kim, Kwang-Ho;Soh, Kyung-Sun
    • Journal of Pharmacopuncture
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    • v.5 no.2
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    • pp.6-24
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    • 2002
  • This experiment was carried out to study on the safety assessment of Bos tures$^{\circ}{\S}Ursus thibetanus^{\circ}{\S}$Moschus extract solution(BUM) for Herbal-acupuncture. SD rats and ICR mice were used for acute toxicity test. the results were summerized as follows; 1. In rats and mice, LD50 value could not be measured. 2. There were no abnormal finding in acute toxicity test treated BUM for Herbal-acupuncture

Review Newly Adopted OECD Acute Oral Toxicity Test Guideline 420 (OECD test guideline 420 고정용량 급성경구독성시험법에 대한 고찰)

  • 정용현;유일재
    • Toxicological Research
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    • v.17 no.3
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    • pp.195-201
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    • 2001
  • The OECD acute toxicity guideline has been revised recently to protect animal welfare. The GLP authority of the Ministry of Environment, the National Institute for Environmental Research, recommended GLP laboratories in Korea to ufo the revised acute toxicity guideline. This study was carried out to optimize newly adopted OECD test guideline 420 (TG 420). Bisphenol A was selected for test chemical. Following TG420, Bisphenol A was classified as class 5/unclassified group. The revised TG 420 was very effective test in minimizing animal number and classifying chemicals. The method, however had short-coming in evaluation of test results statistically because the test had no control group, and the test should be stopped when animals were dead at the lowest dose or alive at the highest dose. TG 420 required at Least 20 animals to complete the test, but it could result in producing unused animals that need to sacrifice.

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Acute toxicity of DA-3030(G-CSF) in rats and mice (랫드와 마우스에서 DA-3030(G-CSF)의 급성독성에 관한 연구)

  • 이영순;조재진;김영석;남정석;박재학;이순복
    • Biomolecules & Therapeutics
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    • v.2 no.3
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    • pp.256-259
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    • 1994
  • This study was performed to evaluate the acute toxicity of DA-3030(granulocyte-colony stimulating factor, G-CSF) in mice and rats via intragastrical and intravenous routes. DA-3030(G-CSF) in the acute toxicity study did not induce any toxic signs in the mice and rats in mortalities, clinical findings, body weights and gross findings. It is suggested that LD$_{50}$ values in mice and rats would be >13, 800 $\mu\textrm{g}$/kg in the oral route and >6, 900 $\mu\textrm{g}$/kg in the intravenous route.e.

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Acute Toxicity Study of Recombinant Granulocyte-Macrophage Colony Stimulating Factor (LBD-005) in ICR mice

  • Kim, Hyoung-Chin;Song, Si-Whan;Cha, Shin-Woo;Shin, Chun-Chul;Ha, Chang-Su;Han, Sang-Seop
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.270-274
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    • 1993
  • The acute toxicity of a recombinant granulocyte-macrophage colony stimulating factor (code name: LBD-005) was evaluated in both sexes of ICR mice, 5~6 weeks old, by the oral, subcutaneous and intravenous routes of administration. Based on the results of the acute toxicity study, LBD-005 was not considered to induce any toxic effect on the mice in mortalities, clinical findings, body weights and gross findings. It is suggested that $LD_50$ values in mice would be >48 mg/kg in the oral route and >24 mg/kg in the subcutaneous or intravenous route.

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Acute Toxicity of Combined Vaccine (KGCC-95VI) Against Japanese Encephalitis and Hantaan Virus Infection

  • Shin, Kwang-Soon;Kim, Chul-Joong;Yun, Hyo-In;Park, Jong-Il;Cha, Shin-Woo;Shin, Hyeong-Soon
    • Toxicological Research
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    • v.13 no.3
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    • pp.275-279
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    • 1997
  • The acute toxicity of the combined vaccine (KGCC-95VI) for the prophylaxis against Japanese encephalitis and Hantaan virus infection. recently developed by Korea Green Cross Corporation, was investigated. KGCC-95VI was administered to the Balb /c mice in two routes, orally and subcutaneously, and into the New Zealand White rabbits subcutaneously. $LD_{50}$ was not accessible as there were no deaths in the group treated even at a dose 800 times the expected clinical dose in both animal species. Between the treated and control groups there were no statistically significant differences in body weight changes and clinical signs during the 14-day observation period, and no pathological gross findings. Accordingly KGCC-95VI is considered not to have the acute toxicity in mice and rabbits.

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Acute Oral Toxicity and Pathogenicity of a Potential Probiotic Bacillus sp. A9184 Isolated from Soybean Paste

  • Lim, Jong-Hwan;Park, Byung-Kwon;Kim, Myoung-Seok;Rhee, Man-Hee;Park, Seung-Chun;Yun, Hyo-In
    • Toxicological Research
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    • v.20 no.4
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    • pp.359-363
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    • 2004
  • This study provides more information about the potential toxicological risk of Bacillus sp. A9184 isolated from soybean paste. The evaluation was based on the guidelines of acute oral toxicity/pathogenicity for microbial pesticide and was to get more comprehensive understanding on the acute toxicity of the potential probiotic in Sprgue-Dawley rats. No dead animal was observed in rats after single oral administration with bacteria ($10^8$ CFU per animal). There were neither no treatment-related changes in clinical signs, nor changes in body weight and body temperature as com-pared with the untreated group. All tested animal showed the increase in body weight with time. The results obtained in this study suggest that the potential probiotic, Bacillus sp. A9184, is non-toxic for rat.