• Title, Summary, Keyword: acetylation

Search Result 284, Processing Time 0.057 seconds

Acetylation of Retinoblastoma Like Protein2 (Rb2/p130) in Tumor Tissues

  • Khan, Z.N.;Sabir, M.;Kayani, M.A.;Saeed, M.
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.14 no.4
    • /
    • pp.2255-2258
    • /
    • 2013
  • The activity of Rb proteins is controlled by post-translational modifications, especially through phosphorylation. Acetylation of Rb2/p130 was reported recently in NIH3T3 cells but its physiological relevance in cell cycle control and tumorigenesis is still unknown. Efforts are underway to investigate possible interplay between Rb2/p130 phosphorylation and acetylation. Here we hypothesized that Rb2/p130 acetylation, like p53 acetylation, may play a role in development of the tumor phenotype. The proposed hypothesis regarding acetylation of Rb2/p130 in tumor VS normal cells was found to be true in our case study of 36 tumor samples. Statistical analysis of results suggest strong correlation among Rb2/p130 acetylation and cancer phenotype.

Effect of Low Level of Starch Acetylation on Physicochemical Properties of Potato Starch

  • Wickramasinghe, Hetti Arachchige Mangalika;Yamamoto, Kazuo;Yamauchi, Hiroaki;Noda, Takahiro
    • Food Science and Biotechnology
    • /
    • v.18 no.1
    • /
    • pp.118-123
    • /
    • 2009
  • In order to find out the effect of low level of starch acetylation on physicochemical properties of potato starch, amylose content, digestibility of raw and gelatinized starch, thermal properties, pasting properties, and the swelling power of native and acetylated potato starches were measured. The amylose content was significantly lower in acetylated starch than in their counterpart native starches. Though a tendency in the decrease in digestibility of raw starch was observed with starch acetylation, acetylation did not alter the proportion of readily digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS) of both raw and gelatinized potato starches. No clear increase in the swelling power was observed, however, the peak and onset gelatinization temperatures and the enthalpy required for starch gelatinization decreased with starch acetylation. Peak and breakdown viscosities were reduced due to acetylation of potato starch while final viscosity and set back were increased.

Investigation on the Dimensional Stability of Acetylated Larch (Larix kaempferi) Small Square (아세틸화처리 낙엽송(Larix kaempferi) 소형 각재의 치수안정성 조사)

  • Lee, Won-Hee;Kang, Ho-Yang
    • Journal of the Korea Furniture Society
    • /
    • v.27 no.4
    • /
    • pp.302-308
    • /
    • 2016
  • It has been known that acetylation improves the dimensional stability of wood. Liquid phase acetylation is more popular than gas-phase acetylation for the effectiveness of weight gain of wood. In this study the specimens of domestic red and Korean pines are acetylated in liquid phase and their physical properties, such as density, bending strength, anti-hygroscopicity etc., are analyzed. Acetylation increases the average weights and volume of larch specimens by 11.4% and 3.4%, respectively, and their average oven-dry densities are increased by $0.03g/cm^3$. Acetylation does not influence on Modulus of Rupture (MOR) and Modulus of Elasticity (MOE). The average Percentage Reduction in Hygroscopicity (PRH) and average Percentage Reduction in Water soaking (PRW) of larch specimens are respectively 20.2% and 20.8%. Thus it can be concluded that acetylation improves the dimensional stability of larch specimens.

Association of CYP2E1 and NAT2 Polymorphisms with Lung Cancer Susceptibility among Mongolian and Han Populations in the Inner Mongolian Region

  • Zhang, Jing-Wen;Yu, Wan-Jia;Sheng, Xiao-Min;Chang, Fu-Hou;Bai, Tu-Ya;Lv, Xiao-Li;Wang, Guang;Liu, Su-Zhen
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.21
    • /
    • pp.9203-9210
    • /
    • 2014
  • Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178-1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P<0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

Histone Acetylation in Fungal Pathogens of Plants

  • Jeon, Junhyun;Kwon, Seomun;Lee, Yong-Hwan
    • The Plant Pathology Journal
    • /
    • v.30 no.1
    • /
    • pp.1-9
    • /
    • 2014
  • Acetylation of histone lysine residues occurs in different organisms ranging from yeast to plants and mammals for the regulation of diverse cellular processes. With the identification of enzymes that create or reverse this modification, our understanding on histone acetylation has expanded at an amazing pace during the last two decades. In fungal pathogens of plants, however, the importance of such modification has only just begun to be appreciated in the recent years and there is a dearth of information on how histone acetylation is implicated in fungal pathogenesis. This review covers the current status of research related to histone acetylation in plant pathogenic fungi and considers relevant findings in the interaction between fungal pathogens and host plants. We first describe the families of histone acetyltransferases and deacetylases. Then we provide the cases where histone acetylation was investigated in the context of fungal pathogenesis. Finally, future directions and perspectives in epigenetics of fungal pathogenesis are discussed.

Global Histone H4 Acetylation of IGF1 and GH Genes in Lungs of Somatic Cell Cloned Calves

  • Zhang, L.;Wang, S.H.;Fan, B.L.;Dai, Y.P.;Fei, J.;Li, N.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.19 no.8
    • /
    • pp.1090-1094
    • /
    • 2006
  • Histone acetylation modification is one key mechanism in the regulation of gene activation. In this study, we investigated the global levels of histone H4 acetylation of insulin like growth factor I (IGF1) and growth hormone (GH) genes in the lungs of two somatic cell cloned calves. Data showed the levels of histone H4 acetylation of IGF1 and GH genes vary widely within different gene regions, and, in almost all regions of the two genes, acetylation levels are lower in the aberrant clone than in the normal clone. Thus we suggest that inefficient epigenetic reprogramming in the clone may affect the balance between acetylation and deacetylation, which will affect normal growth and development. These findings will also have implications for improvement of cloning success rates.

Acetylation of s-Triazine Derivatives from Uronic Acids and their Biological Activity (오탄당(五炭糖) Triazine 유도체(誘導體)의 Acetylation 과 생리활성(生理活性)에 관(關)하여)

  • Lee, Chun-Soo
    • Applied Biological Chemistry
    • /
    • v.21 no.2
    • /
    • pp.119-122
    • /
    • 1978
  • Acetylation of triazinylated monosaccharides was carried out. The products were identified by elementary analysis. IR and NMR with Methyl $[T]-{\beta}-L-acetyl-arabopyranoside$ and Methyl $[T]-\alpha-D-acetyl-lyxopyranoside$ respectively. The triazine derivatives of uronic acids had no remarkable biological activities against dish and rice, and also showed not to be effected on antifungal and antibacterial activities.

  • PDF

NMR Studies on the N-terminal Acetylation Domain of Histone H4

  • Bang, Eun Jeong;Lee, Chang Hun;Yun, Jong Bok;Cheong, Ju Hui;Lee, Dae Yun;Lee, Won Tae
    • Bulletin of the Korean Chemical Society
    • /
    • v.22 no.5
    • /
    • pp.507-513
    • /
    • 2001
  • Histones, nuclear proteins that interact with DNA to form nucleosomes, are essential for both the regulation of transcription and the packaging of DNA within chromosomes. The N-terminal domain of histone H4 which contains four acetylation sites at lysines, may play a separate role in chromatin structure from the remainder of the H4 chain. NMR data suggest that H4NTP peptide does have relating disordered structure at physiological pH, however, it has a defined structure at lower pH conditions. The solution structure calculated from NMR data shows a well structured region comprising residues of Val21-Asp24. In addition, our results suggest that the H4NTP prefers an extended backbone conformation at acetylation sites, however, it (especially Lys 12 ) became more defined structures after acetylation for its optimum function.

  • PDF

Acetylation of Traditional Bamboo Flute (전통 대나무 악기재의 아세틸화)

  • Kang, Ho-Yang;Lee, Jong-Shin;Hur, Jong-Yun;Lee, Gwan-Young;Sim, Jun-Oh
    • Journal of the Korean Wood Science and Technology
    • /
    • v.24 no.3
    • /
    • pp.5-11
    • /
    • 1996
  • It has been reported that acetylation reduces the sorption of wood, thereby increasing the stability of the acoustic and dimensional properties of wood under conditions of changing humidity. Bamboos used for making a Korean traditional flute were acetylated and their dimensional properties were evaluated by measuring Weight Percent Gain and thickness swelling in water. Before acetylation bamboos were vacuum-dried to 3~6% at $50{\sim}80^{\circ}C$ without degradation. The optimum reaction temperature and time for bamboo acetylation were found to be $120^{\circ}C$ and 8 hours, respectively.

  • PDF

Effects of Proto-oncogene Protein DEK on PCAF Localization

  • Lee, In-Seon;Lee, Seok-Cheol;Lee, Jae-Hwi;Seo, Sang-Beom
    • Biomolecules & Therapeutics
    • /
    • v.15 no.2
    • /
    • pp.78-82
    • /
    • 2007
  • The proto-oncogene protein DEK is a nuclear binding phosphoprotein that has been associated with various human diseases including leukemia. Histone acetylation is an important post-translational modification which plays important role in transcriptional regulation. Auto-acetylation of histone acetyltransferase PCAF results in increment of its HAT activity and facilitation of its nuclear localization. In this study, we report that DEK inhibits PCAF auto-acetylation through direct interaction. The C-terminal acidic domains of DEK are responsible for the interaction with PCAF. Using confocal microscopy, we have shown that nuclear localization of PCAF is severely inhibited by DEK. Taken together, our results suggest that DEK may be involved in various cellular signal transduction pathways accommodated by PCAF through the regulation of PCAF auto-acetylation.