• Title, Summary, Keyword: Yeast culture

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Influence of Nutrient Addition in the Liquid Yeast Fermentation of Pulverized Food Wastes (남은 음식물의 습식효모배양에서 영양물질첨가가 효모증식에 미치는 영향)

  • Lee, Ki-Young;Yu, Sung-Jin;Chae, Hee-Jung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.1
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    • pp.49-55
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    • 2001
  • For the production of probiotic feed enriched with viable yeasts, aerobic liquid culture of Kluyveromyces marxianus was attempted in pulverized residual food wastes. After the preliminary shaking culture result, the liquid food wastes was added with urea($0.5g/{\ell}$), o-phosphate($0.4g/{\ell}$ ), molasses($4g/{\ell}$), and yeast extract($1g/{\ell}$), and the fermentation was carried out in 2-litre jar fermenter. In 12 hours of aerobic mixed culture with Aspersillus oryzae, viable cell count of the yeast reached to the number of $1.4{\times}10^{10}/{\ell}$ in the cultured medium.

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Effect of Culture Conditions on Astaxanthin Formation in Red Yeast Xanthophyllomyces dendrorhous Mutant JH1

  • Kim Jeong-Hwan;Choi Seok-Keun;Park Young-Sam;Yun Cheol-Won;Cho Won-Dai;Chee Kew-Mahn;Chang Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.438-442
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    • 2006
  • The formation of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 depends on the culture conditions. Therefore, the effects of inoculation rate (1-5%, v/v) and medium compositions (various carbon and nitrogen sources) on cell growth and astaxanthin formation in X. dendrorhous mutant JH1 were investigated. Inoculation at 3% (v/v) was optimal for cell growth and astaxanthin formation. The most effective carbon source for cell growth and astaxanthin formation was glucose, and the best nitrogen source was yeast extract. The 3% (w/v) glucose and 0.2% (w/v) yeast extract showed the best effect on cell growth and astaxanthin formation, compared with others tested. The 3% glucose, 0.2% yeast extract, $0.15%\;KH_{2}PO_{4}$, $0.05%\;MgSO_4$, $0.01%\;MnSO_4$, and $0.01%\;CaCl_2$ were selected for cell growth and astaxanthin formation. Under the conditions selected, the maximum concentrations of cell and astaxanthin obtained after 168 h of cultivation were 5.43 g/l and 28.20 mg/l, respectively.

Studies on the Production of Microbial Culture Medium by Using By-Product of Salt-Fermented Kanary (까나리 액젓 부산물의 미생물 배지화를 위한 연구)

  • Won, Hye-Jin;Hahm, Young-Tae;Kim, Hye-Kyung;Kim, Byung-Yong
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.202-206
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    • 2000
  • Feasibility of microbial culture media using by-product of salt fermented kanary was investigated. Gram negative strain, Escherichia coli, and Gram positive strain, Bacillus subtilis, and bioluminescent Photobacterium Phosphoreum were incubated with kanary by-Product media (KB media). Compared with LB media, KB media had enough carbon source, but lacked nitrogen source and growth factor. When 0.5% of peptone as a nitrogen source and 0.3% of yeast extract as nitrogen and growth factor source were fortified in KB media, the cell population rate was similar to LB media. Also, when 0.5% of yeast extract was fortified to KB media, it showed the same result as in LB media. The price of KB media with fortification of 0.5% peptone and 0.3% yeast extract, and 0.5% of yeast extract is only 46 and 19% of that of LB media, respectively. These results showed that kanary by-Product could be a good and cheaper bacterial culture media if small amount of nitrogen source and growth factor were added.

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Yeasts in the Flowers of Wild Fleabane [Erigeron annus (L.) Pers.]

  • Kim, Jong-Shik;Kim, Dae-Shin
    • Korean Journal of Environmental Agriculture
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    • v.34 no.3
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    • pp.238-243
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    • 2015
  • BACKGROUND: Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS: We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.

Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense

  • Kim, Jong-Shik;Kim, Dae-Shin;Jeon, Sang-Mi;Ko, Suk-Hyung
    • Korean Journal of Environmental Agriculture
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    • v.35 no.2
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    • pp.137-142
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    • 2016
  • BACKGROUND: Identification of endophytic yeasts inhabiting the internal roots of the Mankyua chejuense tree requires techniques involving biotechnology. There is a need for a culture-based method to isolate and identify yeast strains associated with M. chejuense.METHODS AND RESULTS: We spread homogenized M. chejuense root samples onto glucose-peptone- yeast agar containing antibiotics, Triton X-100, and L-sorbose. A total of 152 yeast isolates were obtained and identified via phylogenetic analysis based on ITS gene sequencing. The results revealed that the root-associated yeast species included the genera Cyberlindnera (140 isolates), Candida (11 isolates), and Kluyveromyces (one isolate). Additionally, three yeast isolates showed high bioethanol production.CONCLUSION: We identified the specific yeast community associated with M. chejuense roots. These yeast isolates may have industrial applications as bioethanol producers. Our findings revealed that Cyberlindnera isolates included C. suaverolens and C. satumus, while Kluyveromyces isolates showed high bioethanol production.

A Study on Environmental Tolerances of Yeast (효모의 환경내성에 대하여 1)

  • 임억규;정영호;김준호
    • Korean Journal of Microbiology
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    • v.16 no.3
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    • pp.93-102
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    • 1978
  • Saccharomyces cerevisiae strain M was cultured in a molasses-containing media with repeated transplantations of the yeasts from one culture to another to adapt to molasses. After that only different amounts of phosphorous and nitrogen sources were added to the media. And then some variations during the culture time and the effects of consituents of cell mass on the functional activity and sensitivity of the cell were investigated. The results obtained were summarized as follows : 1. In the same culture condition of yeasts, the carbohydrates and trehaloses contents were more remarkably increased when small amounts of phosphate and nitrogen sources were added, then when alrge amounts were added, but yield percentage on assimilated sugars was lower. 2. The content of trehalose in yeast cells was reduced remarkably at the early stage in the culture, but this increased remarkably at later stage. When small amounts of nitrogen and phosphate were added to the culture medium, the amount of thehalose in the cells increased greasly. 3. The more protein content was present in the yeast cells, the smaller the carbohydrate and trehalose content, but more amino-N, RNA and moisture content were present in the cells. And in this case fermentability of the cells was stronger, but sugar tolerance was lower. 4. During the preservation period of compressed yeast cells at different temperature, the higher the temperature was, the more rapidly the amount of trehalose in the cells decreased. And in the cell where the amount of trehalose in the cells decreased. And in the cell where the amount of trehalose(carbohydrate) was large and the amount of protein was small, the amount of trehalose decreased at a slower rate during the preservation period.

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Candida tropicalis DS-72에 의한 Xylose로부터 Xylitol의 생산

  • 오덕근;김상용
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.311-316
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    • 1997
  • A high xylitol producing yeast was isolated from the sludge of xylose manufacturing factory and then identified as Candida tropicalis DS-72 according to physiological properties. The strain was able to produce xylitol in a high concentration up to 72g/l from 100g/l xylose in 32 hours. Medium optimization for xylitol production by C. tropicalis DS-72 was performed. Effect of various nitrogen sources on xylitol production was investigated. Of nitrogenous compounds, yeast extract was the most suitable organic nitrogen nutrient for the enhancement of xylitol production. However, inorganic nitrogen resulted in a low cell concentration and did not produce xylitol. Effect of inorganic salts such as KH$_{2}$PO$_{4}$, and MgSO$_{4}$, 7H$_{2}$O on xylitol production was also studied. Optimal medium was selected as xylose 100g/l, yeast extract 10g/l, KH$_{2}$PO$_{4}$, 5 g/l and MgSO$_{4}$, 7H$_{2}$O 0.2 g/l. Xylitol of 88 g/l was produced from 100 g/l xylose in 30 hours using the optimal medium in a flask. In a fermentor, a fed-batch culture with 300g/l xylose was carried out. A final xylitol concentration of 240 g/l in the culture could be obtained in 43 hours of culture time by maintaining the high level of dissolved oxygen during growth phase and limiting the dissolved oxygen in the same culture during production phase. This result corresponded to a xylitol yield of 80% and a xylitol productivity of 5.58 g/1-h.

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Optimum Conditions for the Biological Production of Lactic Acid by a Newly Isolated Lactic Acid Bacterium, Lactobacillus sp. RKY2

  • Wee Young-Jung;Kim Jin-Nam;Yun Jong-Sun;Ryu Hwa-Won
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.1
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    • pp.23-28
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    • 2005
  • Lactic acid is a green chemical that can be used as a raw material for biodegradable polymer. To produce lactic acid through microbial fermentation, we previously screened a novel lactic acid bacterium. In this work, we optimized lactic acid fermentation using a newly isolated and homofermentative lactic acid bacterium. The optimum medium components were found to be glucose, yeast extract, $(NH_4)_{2}HPO_4,\;and\;MnSO_4$. The optimum pH and temperature for a batch culture of Lactobacillus sp. RKY2 was found to be 6.0 and $36^{\circ}C$, respectively. Under the optimized culture conditions, the maximum lactic acid concentration (153.9 g/L) was obtained from 200 g/L of glucose and 15 g/L of yeast extract, and maximum lactic acid productivity ($6.21\;gL^{-1}h^{-1}$) was obtained from 100 g/L of glucose and 20 g/L of yeast extract. In all cases, the lactic acid yields were found to be above 0.91 g/g. This article provides the optimized conditions for a batch culture of Lactobacillus sp. RKY2, which resulted in highest productivity of lactic acid.

Studies on the Production of Yeast. (Part 1) Yeast Production from the Hydrolyzate of Sweet Potato Starch Cake as a Carbon Source (효모생산에 관한 연구(제1보) 고구마전분박 산당화액을 이용한 효모생산)

  • 양한철;최용진;성하진
    • Microbiology and Biotechnology Letters
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    • v.2 no.2
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    • pp.95-101
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    • 1974
  • Studies on the optimum conditions of acid hydrolysis of sweet potato starch cake and its utilization on the production of Saccharomyces cerevisiae as a carbon source were conducted and the results showed as follows; 1.The highest hydrolysis rate, 62.7 % of the reducing sugar based on the weight of the dry matter, was obtained when the starch cake was hydrolyzed with 1.0% of hydrochloric acid at 2.0 kg/$\textrm{cm}^2$ for 30 minutes. 2. But the yeast grew most favorably on the hydrolyzate obtained by treating the starch cake with 0.5% of hydrochloric acid at 2.0 kg/$\textrm{cm}^2$ for 10 minutes. Reducing sugar content of hydrolyzate was 51.4%. 3. The optimum pH of the culture medium was 7.0, Cell growth reached to the maximum at 36 hours of cultivation time. 4. According to the vitamin requirement tests, Ca-pantothenate was found to be a promoting factor for the growth of the yeast cells. 5. "Gluten acid hydrolyzate" was most effective to the cell growth when added to the medium at the concentration of 0.1% as a nitrogen source. 6. Sacch. cerevisiae could assimilate the sugars in the hydrolyzate about 89.1%, and the yields of the yeast cells showed 23.2mg/ml of culture medium.

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Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis

  • Bunsangiam, Sakaoduoen;Sakpuntoon, Varunya;Srisuk, Nantana;Ohashi, Takao;Fujiyama, Kazuhito;Limtong, Savitree
    • Mycobiology
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    • v.47 no.3
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    • pp.292-300
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    • 2019
  • IAA biosynthetic pathways in a basidiomycetous yeast, Rhodosporidiobolus fluvialis DMKU-CP293, were investigated. The yeast strain showed tryptophan (Trp)-dependent IAA biosynthesis when grown in tryptophan supplemented mineral salt medium. Gas chromatography-mass spectrometry was used to further identify the pathway intermediates of Trpdependent IAA biosynthesis. The results indicated that the main intermediates produced by R. fluvialis DMKU-CP293 were tryptamine (TAM), indole-3-acetic acid (IAA), and tryptophol (TOL), whereas indole-3-pyruvic acid (IPA) was not found. However, supplementation of IPA to the culture medium resulted in IAA peak detection by high-performance liquid chromatography analysis of the culture supernatant. Key enzymes of three IAA biosynthetic routes, i.e., IPA, IAM and TAM were investigated to clarify the IAA biosynthetic pathways of R. fluvialis DMKU-CP293. Results indicated that the activities of tryptophan aminotransferase, tryptophan 2-monooxygenase, and tryptophan decarboxylase were observed in cell crude extract. Overall results suggested that IAA biosynthetic in this yeast strain mainly occurred via the IPA route. Nevertheless, IAM and TAM pathway might be involved in R. fluvialis DMKU-CP293.