• Title, Summary, Keyword: Xylanase

Search Result 437, Processing Time 0.034 seconds

Optimization of Xylanase Production from Paenibacillus sp. DG-22 (Paenibacillus sp. DG-22로부터 xylanase 생산의 최적화)

  • Lee, Yong-Eok
    • Journal of Life Science
    • /
    • v.13 no.5
    • /
    • pp.618-625
    • /
    • 2003
  • Investigations were carried out to optimize the culture conditions for the production of xylanase by Paenibacillus sp. DG-22, a moderately thermophilic bacterium isolated from timber yard soil. Xylanase production showed a cell growth associated profile. Xylanase activity was found only in the culture supernatant, while $\beta-xylosidase$ activity was mainly associated with the cells. The formation of xylanase activity was induced by xylan and repressed by glucose and xylose. The production profile of xylanase was examined with various commercial xylan and maximum yield was achieved with 0.1∼ 0.5% birchwood xylan. Among various nitrogen sources tested, yeast extract was optimal for the production of xylanase. The xylanase activity was inhibited by $Co^{2+},\; Cu^{2+},\; Fe^{3+},\; Hg^{2+}\;$ and$\;Mn^{2+}$ ions while $Ca^{2+},\; Mg^{2+},\; Ni^{2+},\; Zn^{2+}$ions and DTT stimulated xylanase activity Mercury (II) ion at 5 mM concentration abolished all the xylanase activity. The predominant products of xylan-hydrolysate were xylobiose, xylotriose, and higher xylooligo-saccharides, indicating that the enzyme was an endoxylanase.

Variation of Oak Kraft Pulp Properties by Xylanase Treatment in C/D, P and Z Stage (C/D, P 및 Z단계 표백시 Xylanase처리에 의한 펄프성질의 변화)

  • Kim, Dong-Ho;Paik, Ki-Hyon
    • Journal of the Korean Wood Science and Technology
    • /
    • v.25 no.2
    • /
    • pp.100-109
    • /
    • 1997
  • The objectives of this study was to decrease pollutions of bleaching effluent and was to enhanced brightness of non-chlorine bleached pulps by xylanase treatments. Xylanase cloned Esherichacoli(E. coli) capable of each of endo, exo-xylanase and acetyl-esterase were obtained from Bacillus stearothermophillus. These xylanase was maintained high activity in alkali and high temperature. Especially endo-xylanase would be more active in $60^{\circ}C$ and pH 11. Xylanase pretreatment(X) of unbleached pulp increased brightness, and decreased the degree of delignification. The degree of increase in brightness of pulp due to xylanase pretreatment was similar to non-enzyme treated pulp, regardless of the amount of enzyme added. Therefore, the addition of xylanase of 2 unit was recommended when considering costs of enzyme. The pulp bleached XO sequence had higher brightness and lower Kappa no, than O bleached pulp, while pulp bleached XP sequence had similar brightness and Kappa no. with P bleached pulp. In XOC/D, XOZ and XOP bleaching sequences, brightness and degree of delignification were improved. The C/D and Z stage bleached pulp was good effect on rate of raise in brightness and Kappa no., but P stage bleached pulp had similar level in non-enzyme treated bleaching sequence.

  • PDF

Studies on the Isolation and Identification of Xylanase and Mannanase Producing Aspergillus niger (Xylanase와 Mannanase를 생산하는 Aspergillus niger의 분리와 동정에 관한 연구)

  • Kim, Byoung-Suk;Cho, Jin-Kook;Song, Jin-Ook;Lee, Hak-Kyo;Hwang, Seong-Gu
    • Journal of Animal Science and Technology
    • /
    • v.51 no.5
    • /
    • pp.427-432
    • /
    • 2009
  • This study was undertaken to screen a high xylanase and mannanase producing microbes. In the first experiment, screening was undertaken against 50 samples of microorganisms having xylanase and mannanase activities from soil and fallen leaves. The screening process has focused on picking out fungi having high xylanase and mannanase activities under the solid-state fermentation. The xylanase and mannanase activities of 6 screened microbes were 0.9~1.6 unit/mL and 0.2~0.4 unit/mL, respectively, under the submerged fermentation condition. However, under the solid-state fermentation, xylanase and mannanase activities were 103.7~220.0 unit/g and 20.1~40.3 unit/g, respectively. Finally one microbe (E-3) was selected and its xylanase and mannanase activities were 197.3 unit/g and 39.9 unit/g, respectively. The morphological and molecular biological classification of E-3 showed 99% homology with the Aspergillus niger.

The Character Variation of Wood-Pulp treated Three Enzyme ; Endo-xylanase, Exo-xylanase and Acetyl-esterase (Endo-xylanase, Exo-xylanase 몇 Acetyl-esterase 효소 처리한 펄프의 특성 변화)

  • Kim, Byong-Hyun
    • Journal of the Korean Printing Society
    • /
    • v.26 no.1
    • /
    • pp.17-28
    • /
    • 2008
  • The wood-pulp is treated with the three enzymes; Endo-xylanase, exo-xylanase and acetyl-esterase. The maximum value of relative activity appeared 0.95 in acetyl-esterase at $40^{\circ}C$, 0.9 in exo-xylanase at $40^{\circ}C$, and 0.8 in endo-xylanase at $50^{\circ}C$, respectively. And it has measured 0.8 in endo-xylanase, 0.95 in acetyl-esterase at pH 6 and 0.9 in exo-xylanase at pH 5, while the maximum value of relative activity does not rely on reaction time for three enzymes treatment, and the value was about 0.9, respectively. We have watched that decreased Kappa number and increased brightness. And it turned out that the three enzyme produced a lot of reducing sugar with wood-pulp treatment.

  • PDF

Stiudies on Enzyme of the Thermophilic Mold -(Part 4) Xylanase and Laminaranase from Thermophilic Mold- (고온성 사상균의 효소에 관한 연구 -(제4보) 고온성 사상균의 Xylanase 와 Laminaranase-)

  • Chung, Song-Hyo;Lee, Ke-Ho
    • Applied Biological Chemistry
    • /
    • v.15 no.3
    • /
    • pp.207-211
    • /
    • 1972
  • In order to investigate the action of thermophilic fungi on the xylan and its related substances, xylanase and laminaranase were examined using Myriococcum albomyces. In the extract from bran culture of Myriococcum albomyces, xylanase and laminaranase activity were recognized. 1) The optimum pH for xylanase and laminaranase activity were found to be pH 5.0 and pH 6.0. 2) The optimum temperature for xylanase and laminaranase activity were found to be $55^{\cicr}C$. 3) Thermal stability of xylanase for 55 minutes at $65^{\cicr}C$ and laminaranase for 60 minutes at $65^{\cicr}C$ did not influence their stability.

  • PDF

Purification and Characteristics of Xylanases from Produced Thermophilic Alkalophilic Bacillus K17 (고온, 알칼리성 Bacillus K17이 생성하는 Xylanase의 정제 및 특성)

  • Kang, In-Soo;Sung, Nack-Kie;Chun, Hyo-Kon;Teruhiko Akiba;Koki Horikoshi
    • Microbiology and Biotechnology Letters
    • /
    • v.14 no.6
    • /
    • pp.447-453
    • /
    • 1986
  • The culture filtrate of thermophilic alkalophilic Bacillus K17 strain contained two types of xylanases were purified by ammonium sulfate fractionation, DEAD-Sephadex A-50 column chromatography, CM-Sephadex C-50 column chromatography and Sephadex G-100 gel filtration. The purified enzymes were found to be homogeneous by sodium dodecyl sulfate and disc polyacrylamide gel electrophoresis. Xylanase I and II were characterized with respect to molecular weight, optimal temperature and pH, thermal and pH stability, and Michaelis constant. Xylanase II was more active and stable, and showed greater substrate affinity and molecular weight than xylanase I. The activities of xylanases I and II were inhibited by Cu$^{++}$, Ag$^+$, Hg$^{++}$ and Fe$^{++}$. Xylanase I hydrolyzed xylan to yield xylobiose and higher amount of xylooligosaccharides, but xylanase II produced xylose other than xylobiose and xylooligosacchrides.

  • PDF

Isolation and Identification of Exo-xylanase Producing Microorganism (Exo-xylanase 생산균의 분리 및 동정)

  • 하재석;이영남;임재윤
    • Microbiology and Biotechnology Letters
    • /
    • v.20 no.1
    • /
    • pp.14-19
    • /
    • 1992
  • The xylanase producing microorganisms occurring on rotten woods were selectively isolated on the modified Czapek-Dox medium supplemented with 0.5% xylan as a sole carbon source. Among more than three-hundred isolates of xylanase producing microorganisms, only two bacterial isolates were turned out to be more potent xylanase producer than the reference strain of xylanase producer, Aureobaszdium pullulans NRRL Y-2311. The exo-xylanase producer, bacterial isolate No. 33 was identified as a strain of Pseudomonas sp. on the basis of morphological and biochemical characterizations as well as cellular fatty acid composition. Optima of pH and of temperature for enzyme reactions of xylanase were 5.5 and $50^{\circ}C$ respectively. The enzyme was stable in a range of pH 5.0~7.0 and below $45^{\circ}C$. Among the number of carbohydrate substrates, xylose was turned out to be a potent inducer of Pseudomonas sp. No.33 exo-xylanase. Among the raw materials tested, rice straw was the best material for xylanase production by Pseudomonas sp. strain No. 33.

  • PDF

Purification and Characterization of Xylanase from Bacillus sp. A-6 (Bacillus sp. A-6의 Xylanase의 정제와 특성)

  • Choi, Suk-Ho
    • Microbiology and Biotechnology Letters
    • /
    • v.37 no.2
    • /
    • pp.147-152
    • /
    • 2009
  • A xylanase was purified from the culture supernatant of Bacillus sp. A-6 by using ultrafiltration and ion exchange chromatography on the column of SP-Sepharose using 5 mM acetate buffer, pH 5.0. The xylanase was eluted from the column at the concentration less than 0.05 M NaCl. The eluted xylanase was shown to be a single protein band in SDS-PAGE. Zymogram analysis indicated that the protein band in SDS-PAGE had the enzyme activity to hydrolyze oat spelt xylan. The molecular weights of the xylanase were 15,000 based on SDS-PAGE and 14,100 based on gel filtration chromatography. Thin layer chromatography showed that the xylanase hydrolyzed oat spelt xylan into xylobiose and high-molecular-weight xylooligosaccharides. The relative activities of the heated xylanase decreased to 80% at $40^{\circ}C$ after 7 hr and less than 40% at $60^{\circ}C$ after 1 hr.

Isolation and Characterization of Xylanase-producing Paenibacillus sp. HY-8 from Moechotypa diphysis (털두꺼비하늘소 (Moechotypa diphysis)로부터 Xylanase를 생산하는 Paenibacillus sp. HY-8 균주의 분리 및 특성)

  • Heo, Sun-Yeon;Oh, Hyun-Woo;Park, Doo-Sang;Kim, Hyang-Mi;Bae, Kyung-Sook;Park, Ho-Yong
    • Korean journal of applied entomology
    • /
    • v.46 no.2
    • /
    • pp.303-311
    • /
    • 2007
  • From the course of screening of useful xylanase producing microorganism from a phytophagous longicorn beetle, we isolated an extra-cellular xylanase producing strain, Paenibacillus sp. HY-8 from the intestine of Moechotypa diphysis adult. On the basis of morphological, biochemical and phylogenetic studies of the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. The highest xylanase production was attained in the M9 media containing 1% yeast extract and 0.5% birchwood xylan when cultured at $25^{\circ}C$ for 24 hrs. HY-8 producing xylanase showed superior hydrolytic activities against various plant source feedstuff than control xylanase produced by Tricoderma sp. at pH 6.0.

Xylanase Production from Bacillus safensis Isolate by Xylan or Xylan Hydrolyzed Products (Xylan과 Xylan 가수분해물에 의한 Bacillus safensis 분리균의 Xylanase 생산)

  • Jin, Hyun Kyung;Yoon, Ki-Hong
    • Microbiology and Biotechnology Letters
    • /
    • v.44 no.3
    • /
    • pp.324-332
    • /
    • 2016
  • A bacterial strain capable of hydrolyzing xylan was isolated from fermented soybean paste obtained from a domestic Buddhist temple, using enrichment culture with rice straw as a carbon source. The isolate, named YB-1301, was identified as Bacillus safensis on the basis of its DNA gyrase subunit B gene (gyrB) sequence. The xylanase productivity of strain YB-1301 was drastically increased when it was grown in the presence of wheat bran or various xylans. In particular, the maximum xylanase productivity reached above 340 U/ml in the culture filtrate from LB broth supplemented with only birchwood xylan at shake-flask level. The xylanase production was significantly induced by xylans at the stationary growth phase in LB medium containing xylan, whereas only a small amount of xylanase was constitutively produced from cells grown in LB medium with no addition of xylan. Furthermore, xylanase biosynthesis was induced more rapidly by the enzymatically hydrolyzed products of xylan than by the non-hydrolyzed xylan. In addition, the xylanase in the culture filtrate of B. safensis YB-1301 was found to have optimal activity at 55℃ and pH 6.5–7.0.