• Title, Summary, Keyword: Vorticella

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Cyst formation of the marine ciliate, Vorticella sp. using MCCF medium (MCCF 배지를 이용한 해산 섬모충 Vorticella sp.의 cyst형성)

  • JUNG Min-Min;RHO Sum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.3
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    • pp.317-319
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    • 1999
  • The ciliate, Vorticella was often observed in the rotifer mass culture tanks as common co-existing organism. This Vorticella performed as a predator for aquatic bacteria population in the rotifer mass culture tanks. This study was carried out to investigate a cyst formation medium of Vorticella in the laboratory for keeping Vorticella seed. The test organism Vorticella sp. was isolated from culture water of rotifer mass culture tanks. The cyst of Vorticella was formed by dried-method for the formation and maintainance of cyst. MCCF (Marine Ciliate Cyst Formation) medium was used for cyst formation (incystment), preservation and return to moving cell (excystment) of the marine ciliate, Vorticella sp. The cyst shape and size were ellipical type and $30.51 \pm1.98\;\mu$m (Avg. $\pm$ SD) of minor axis and $28.89 \pm2.12\;\mu$m (Avg. $\pm$ SD) of minor axis (n=10), The Vorticella cyst was kept in the room temperature ($10\~35^{\circ}C$) and total dark condition (24D:0L) during 1 year. The preserved cyst was transferred to moving cell state (excystment) only by the addition of fresh sea water in the MCCF medium. The five Vorticella sp. moving cells of excysted from cysts showed the growth up to 912$\pm$64 cells/10 ml in MCCF medium during the culture period of 16 days. This MCCF medium was very useful tool for cyst formation and species preservation of marine ciliate Vorticella.

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Biological Application of Two Protozoan Species, Euplotes sp. and Vorticella sp., for the Stable Culture of the Rotifer Brachionus rotundiformis in Laboratory Experiments of Inter- and Tripartite-Specific Relations

  • Jung, Min-Min
    • Fisheries and aquatic sciences
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    • v.15 no.3
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    • pp.209-213
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    • 2012
  • Members of the ciliate group of protozoans are often observed in mass cultures of rotifers. In particular, Euplotes and Vorticella are common contaminating species. In this study, I examined the effect of the ciliates Euplotes sp. and Vorticella sp. on the growth of the rotifer Brachionus rotundiformis by conducting inter-specific and tripartite-specific mixed-culture experiments. The growth of rotifers was suppressed in co-existence with Euplotes sp. compared with monocultures of rotifers. However, Vorticella sp. promoted rotifer growth. Moreover, Vorticella sp. improved the growth of rotifers suppressed by Euplotes sp. contaminants. In 5-L semi-mass cultures of rotifers, growth of the contaminating protozoan Euplotes sp. was heavily suppressed by Vorticella sp. The stable maintenance of the rotifer culture ecosystem can be achieved by manipulating the types of contaminating protozoan species.

Two Newly Recorded Vorticellid Species (Ciliophora, Oligohymenophorea, Peritrichia) from Jindo Island with Other Populations in Korea

  • Kim, Ji Hye;Shin, Mann Kyoon
    • Animal Systematics, Evolution and Diversity
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    • no.spc9
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    • pp.10-17
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    • 2016
  • Carchesium polypinum and Vorticella campanula were collected in freshwater ponds and reservoirs on Jindo Island and other places in Korea. These two vorticellids are newly recorded in Korea. The two species are described using live and silver impregnated cell observations, morphometry, line drawings, and microphotographs. Characterizations were based on the following diagnostic features: Carchesium polypinum was observed mainly as a funnel to fanshaped colony, basically dichotomous branching pattern, discontinuous spasmoneme, subconical shaped zooid, J shaped macronucleus, ventrally positioned single contractile vacuole, and pellicular striation with 61-80 rows between the peristomial lip and aboral ciliary wreath, and 18-30 rows between the aboral ciliary wreath and scopula. Vorticella campanula was conspicuously characterized by the broadly bulged peristomial lip, wide conical shaped zooid, J or S shaped macronucleus, ventrally positioned single contractile vacuole and pellicular striation with 64-75 rows between the peristomial lip and aboral ciliary wreath, and 27-41 rows between the aboral ciliary wreath and scopula. We provide redescriptions of two well-known vorticellids with detailed drawings and descriptions of oral ciliatures from Jindo Island and other places on the Korean peninsula.

Taxonomic and Ecologic Studies on Ciliate Plankton in Pal'tang Reservoir, Korea (팔당호 섬모충 플랑크톤의 분류 및 생태학적 연구)

  • Moon, Eun-Young;Kim, Young-Ok;Kim, Baik-Ho;Kong, Dong-Soo;Han, Myung-Soo
    • Korean Journal of Ecology and Environment
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    • v.37 no.2
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    • pp.149-179
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    • 2004
  • In order to understand the taxonomic and ecologic characteristics on ciliates plankton, surface water samples were collected once a weekly from March 2001 to December 2001 except once a monthly from December 2001 to January 2001 and twice a monttdy at February 2001 at Pal'tang reservoir in Korea. Totally 12 species belonging to 10 genera in 7 families of 6 order, were identified and all species new record in Korea. All of them had been recorded in eutrophic water bodies. Succession of dominant species occurred obviously according to seasonality. Large -sized Codonella cratera, Rimostrombidium lacustris, Strobilidium caudatum, Limnostrombidium viride, Pelagostrombidium fallax, Stylonychia sp., Pseudostrombidium plaktonticum and Phascolodon vorticella dominated during the period of outbreak of nanophytoplankton in winter to spring. However, small-sized Rimostrombidium hyalinum, rimostrombidium brachykinetum, Halteria gradinella and Vorticella convallaria occurred when the bacteria abundance increased in summer to autumn.

Seasonal Succession of Planktonic Ciliate in Kyungan Stream of Lake Paldang, Korea (팔당호 유입부 경안천의 섬모충 플랑크톤 계절적 분포)

  • Moon, Eun-Young;Kim, Young-Ok;Kong, Dong-Soo;Han, Myung-Soo
    • Korean Journal of Ecology and Environment
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    • v.41 no.1
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    • pp.11-18
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    • 2008
  • Seasonal succession and community composition of planktonic ciliates were studied in Kyungan Stream from December 2000 to December 2001. Oligotrichs accounted for 53% of total ciliates. Total abundance of ciliates peaked in spring (30 March, 6 April) and in summer (8 June, 20 July) reaching values up to $1.9\times10^4$ cells $L^{-1}$. Seasonal succession of dominant species occurred obviously. Large-sized $(>50{\mu}m)$ species (Stylonychia sp1, Phascolodon vorticella and Codonella cratera) dominanted from winter to spring. Small sized $(<30{\mu}m)$ species (Vorticella spp., Rimostrombidium hyalinum and Halteria grandinella) dominanted in summer and autumn. Total abundance of large-sized species coincided with the Chl-${\alpha}$ concencetation during the study (r=0.33, p<0.05, n=39). Among the small-sized species Halteria grandinella was a significant relationship with bacterial abundance (r=0.35, p<0.05, n=39).

Efficient Treatment of Sewage Sludge by Effective Microorganisms (유효미생물에 의한 하수슬러지의 효율적 처리)

  • Choi, Chung-Sig;Joo, Gil-Jae;Lee, Dong-Hoon;Choi, Choong-Lyeal;Rhee, In-Koo;Choi, Jyung
    • Current Research on Agriculture and Life Sciences
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    • v.17
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    • pp.45-52
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    • 1999
  • This study was conducted to evaluate the effects of microbial inoculation on sewage sludge composting. The number and species of microorganisms in sewage sludge sampled on February were higher than those sampled on August. The composting of sewage sludge is inhibited by the polyacrylamide cation, which was used as a coagulant and known to repress the growth of microorganisms. The growth of all microorganisms was inhibited by the addition of the polyacrylamide cation at a concentration of more than 0.8%. The species and viable counts of microorganisms were observed to increase during composting sewage sludge by inoculation of the effective microorganisms and addition of the pine tree sawdust as a bulking agent, compared with those without inoculation. A variety of organisms in compost(sewage sludge plus sawdust) were observed after composting for 30 days, such as Fragilaria sp., Proales sp., Vorticella sp., Schizothrix sp., Anabaena sp., Zoothaminium sp., Epstylis sp., Arcella sp., Balantidium sp., Actinophrys sp., Synedra sp., Euglypha sp., Ulothrix sp., Anacystis sp., and Clostium sp.

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New record of five ciliates(Protozoa, Ciliophora) collected in eastern Gangwon-do Province, South Korea

  • Kim, Ji Hye;Jung, Jae-Ho
    • Journal of Species Research
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    • v.7 no.2
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    • pp.181-186
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    • 2018
  • We investigated five ciliate species from soil, estuary and marine habitats in eastern Gangwon-do Province, South Korea. Morphology was studied through observation of living specimens as well as silver preparations. The species were classified into five genera, five families, five orders and four classes. Aspidisca aculeata, Hemiurosoma similis, Litonotus alpestris, Stentor roeselii and Vorticella infusionum are first records in Korea, and among them, the genus Hemiurosoma is recorded for the first time. Here, we provide short descriptions with microphotographs of these species.

Isolation and Characterization of Benzene-degrading Bacteria. (Benzene 분해 세균의 분리와 특성연구)

  • 김정현;유재근;이형환
    • Microbiology and Biotechnology Letters
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    • v.16 no.5
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    • pp.379-383
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    • 1988
  • To evaluate the treatability of activated sludge induced by benzene with microorganisms, isolation and characterization of benzene-degrading microorganisms were carried out. Six bacterial isolates from the activated sludge were identified ; Pseudomonas fluorescens, Enterobacter agglomerans, Enterobacter cloacae, Klebsiella oxytoca, Citrobacter freundii and Klebsiella pneumoniae. P. fluorescens degraded 55% of benzene contained in the medium as a sole carbon source, E. cloacae 24%, E. agglomerans 41%, and K. oxytoca 32%. Optimal temperature, pH and benzene concentration for growth of P. fluorescens appeared to be 31$^{\circ}C$, pH 7.0, and 300mg benzene per liter. When the P. fluorescens was dominant in the activated sludge induced by benzene, the indicator protozoa was Aspidisca sp. When concentration of benzene was about 387mg per liter, the growths of Aspidisca sp. and Litonotus sp. were high. Protozoa, Litonotus sp. and Vorticella sp. did not grow over 1600mg of benzene per liter.

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Protozoa as an Indicator of Activated In Sludge Plant Effluent Quality (원생동물을 이용한 하수처리장의 수질 예측)

  • 이찬형;문경숙
    • Microbiology and Biotechnology Letters
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    • v.28 no.6
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    • pp.361-366
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    • 2000
  • Genera and number of protozoa were investigated in the conventional activated sludge pilot plant used for the treatment of municipal sewage and pre-treated night soil-containing sewage. In both case, the predominant protozoa was ciliates and among them Vorticella was the most common. In the pilot plant where pre-treated night soil was mixed with municipal sewage, genera of free-swimming ciliates, flagellates and amoeba was higher than in those withour night soil. Correlation analysis on the quality of effluent and protozoa indicates that municipal sewage has positive correlation with protozoa. However in the pilot plan 샐 sewage contatinin pre-treated night coil soil more samples show negative correlation. Followed equations were derived by the regression analysis of BOD in both the pilot plants. In case of pilot plant A of municipal sewage, the analysis B of munici-pal and pre-treated night soil-containing sewage, the analysis of BOD was $6.731$\times$10_{-2}$ $\times$Bodo+0.306(Adjusted $R^2$=0.864). At low temperature, number of protozoa was decreased to 35% and among therm, Aspidisca was the most common genus. Therefore, protozoa can be used as indicator of quality of the effluent in sewage treatment plants.

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Characteristics of Microfauna in Biological Treatment of Landfil Leachate with Reactor Including Porous Media (다공성 Media가 조여된 반응조를 이용한 매립지 침출수의 호기성 생물학적 처리시 미소생물상의 특성)

  • 홍성철;박연규
    • Journal of Environmental Science International
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    • v.5 no.1
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    • pp.61-69
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    • 1996
  • The combined wastewater of municipal landfill leachate and municipal sewage was treated using several sets of bench-scale aerated circulating system including porous media. Investigated items in this experiment were the dominant protozoa and metazoa in this system, the variation of microfauna relationship between operating condition and dominant genera. Also considered the factors determining dominant genera and their role. The outcome of this research is as follows; 1. Aspidisca, Vorticella, Truhellophyllum, Lecane, Philodina, Cyclops were mainly appeared prior to combinding leachate, while Trachelocerca, Bodo, Glaucoma were the dominant genera after combinding leachate. 2. As to metazoa, Nematode and Philodina were not influenced by 5oA leachate mixing ratio, meanwhile Crustacea has high sensitivity for increased leachate mixing ratio and it was not appeared in 5% leachate mixing ratio. 3. The appropriate treatability could'nt be expected at the above 10% leachate mixing ratio. Especially, in the condition of 20% leachate mixing ratio, all of the microfauna were affected damage seriously on their existence. Meanwhile hydraulic retention time, substrate loading rate and slut자e production rate didn't give notable influence on increasing the number of microfauna. 4. As to protozoa, saprozoic and holozoic species were appeared commonly and polysaprobic species were dominent. 5. Filamentous organsms were nearly not affected by leachate mixing. It seems that they could live without any trouble at the 10% leachate mixing ratio, if the substrate is sufficient. 6. Diversity of microfauna had a reducing trernd as the sewage was mixed with leachate.

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