• Title, Summary, Keyword: Viscum album

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Preparation of Alginate/Chitosan Microcapsules and Enteric Coated Granules of Mistletoe Lectin

  • Lyu, Su-Yun;Kwon, Young-Ju;Joo, Hye-Jin;Park, Won-Bong
    • Archives of Pharmacal Research
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    • v.27 no.1
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    • pp.118-126
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    • 2004
  • The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cytotoxic and immune-system-stimulating activities. Korean mistletoe (Viscum album L. coloratum), a subspecies of European mistletoe, has also been reported to possess anticancer and immunological activities. A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. coloratum agglutinin, VCA) with Mr 60 kDa was isolated from Korean mistletoe. Mistletoe preparations have been given subcutaneously due to the low stability of lectin in the gastrointestinal (GI) tract. In the present study, we investigated the possibility of alginate/chitosan microcapsules as a tool for oral delivery of mistletoe lectin. In addition, our strategy has been to develop a system composed of stabilizing cores (granules), which contain mistletoe lectin, extract or powder, coated by a biodegradable polymer wall. Our results indicated that successful incorporation of VCA into alginate/chitosan microcapsules has been achieved and that the alginate/chitosan microcapsule protected the VCA from degradation at acidic pH values. And coating the VCA with polyacrylic polymers, Eudragit, produced outstanding results with ideal release profiles and only minimal losses of cytotoxicity after manufacturing step. The granules prepared with extract or whole plant produced the best results due to the stability in the extract or whole plant during manufacturing process.

Quality Characteristics and Antioxidant Activities of Yanggaeng Added with Viscum album Extracts (겨우살이 추출물 첨가 양갱의 품질특성 및 항산화활성)

  • Yoo, Sujung;Yoo, Dongjin;Kim, Changeun;Kim, Soohyun
    • The Korean Journal of Community Living Science
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    • v.28 no.1
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    • pp.93-101
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    • 2017
  • This study was conducted to assess the quality characteristics and antioxidant activity of yanggaeng prepared with different concentrations of Viscum album extracts (VAE; 0, 1, 3 and 5%). The moisture content ranged from 37.85% to 39.38%, exhibiting no significant differences between the groups. The pH level of VAE 5% added yanggaeng was the lowest, followed in order by 3% and 1% additions. Increasing the amount of VAE in yanggaeng tended to increase acidity. The lightness value of the Hunter color system decreased based on the amount of VAE concentrate added to yanggaeng. As VAE content increased, changes in hardness, gumminess, and brittleness were all significant (p<0.05). Characteristics of cohesiveness and springiness showed no significant differences. Total polyphenol content was the highest in VAE 5% added yanggaeng. Antioxidant activities such as DPPH radical scavenging activity of the control group was 2.29%, whereas groups with added VAE ranged from 7.52~28.05%. As VAE increased, antioxidative activity also increased. In the sensory evaluation, yanggaeng addition with VAE 3% had excellent scores for bitterness, moistness, and overall acceptability. Yanggaengs with moderate levels of VAE 3% are recommended (with respect to overall preference score) to take advantage of the functional properties of VAE without sacrificing consumer acceptability.

Effect of Harvesting Time and Storage Duration of Viscum album Seeds on in vitro and ex vitro Germination on the Branch of Prunus mume

  • Lee, Hyun Woo;Ghimeray, Amal Kumar;Lee, Bo-Duk;Sharma, Pankaja;Shim, Ie Sung;Park, Cheol Ho
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.256-262
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    • 2014
  • Viscum album var. coloratum (mistletoe) is considered as one of the endangered plant species in Korea. Our objective is to restore its population and multiplication of plant by ex situ method. In this research we explored the maximum germination (in vitro) of freshly harvested and stored seeds of mistletoe collected in different time intervals. Ex vitro germination after artificial inoculation on the branches of Prunus mume in different physiological conditions was also monitored. The research revealed that the lately harvested seeds (Feb. and March 2014) were superior over early harvested seeds (Nov. 2013 and Jan. 2014) of mistletoe due to the higher percentage of germination (above 93%). According to the data, it is also revealed that the survival and germination rate of mistletoe seeds decreased with the increase in storage duration. In ex vitro germination, the fluctuated temperature of a glass house in natural condition enhanced (four fold) the rate of germination on the branches of Prunus mume than the constant temperature condition in the glass house.

Germination and Seedling Induction of Viscum album var. coloratum (Kom.) Ohwi after Artificial Inoculation on the Branch of Host Plants (겨우살이의 종자의 기주목 접종 및 유묘 활착기술)

  • Kim, Chul-Woo;Yi, Jae-Seon
    • Journal of Forest and Environmental Science
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    • v.29 no.2
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    • pp.173-180
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    • 2013
  • Berries of Korean mistletoe (Viscum album var. coloratum [Kom.] Ohwi) contained one seed, which have, in general, one or two embryos but very rarely three embryos. Mucilaginous substances in berries may help them adhere to the branches of host trees. It was observed that seeds need more than one and half years to develop into normal and healthy seedlings from the time of inoculation. Many factors such as adhesion of berry, thickness of host branch, orientation of haustorial root, etc. influenced the successful development of mistletoe plants. Through the application of six-year observation results on the germination of seeds and growth of seedlings, about 80% of germination rate for mistletoe seeds and 61% of survival ratio for germinated seeds, which is more than 23 times higher in natural conditions, were obtained after inoculation of seeds on the one-year-old branches of Malus pumila var. dulcissima and Quercus mongolica trees. The technological aspects of the success can be applied to other host plants and provide a critical clue to an artificial propagation system, for this medicinally valuable genus. This is the first successful report on artificial inoculation and plant development of Korean mistletoe.

Antifungal Activities of meOH Extracts from Three Korean Mistletose against Tyromyces palustris, Endothia nitschkei and Trichophyton rubrum (3종의 한국산 겨우살이 메탄올 추출물의 Tyromyces palustris, Endothia nitschkei 그리고 Trichophyton rubrum에 대한 항균활성)

  • 안원영;최원실;박미진
    • Journal of Korea Foresty Energy
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    • v.19 no.1
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    • pp.49-60
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    • 2000
  • The traditional Korea medical book already recorded various biological activities of the Korean mistletoes. The objective of this study was examine antifungal activities of MeOH extract from the Korean mistletoe through column chromatography on three fungi, such as Tyromyces palustris Endothia nitschkei and Trichophyton rubrum. No mistletoes had anti-fungal activity against T. palusties. Extracts of V. album var. coloratum showed the highest hyphal growth-inhibitory activity against E. nitschkei and leaf extract of this species had higher activity than twig extract. Further fractionation of most active fraction and following antifungal assay showed that its anti-fungal activity might be caused by synergism if its components. It was suggested that Viscum album var. coloratum shows significantly antifungal activities against E. nitschkei and T. rubrum. Further examination is needed to find out more exact active compounds.

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Inhibitory effects of the extract of Viscum album on the proliferation of human tumor cell lines

  • Seo, Jee-Hee;Choi, Yeon-Hee;Kim, Jung-Sook;Kim, Seong-Kie;Choi, Sang-Un;Kim, Young-Sup;Ryu, Shi-Yong;Kim, Young-Kyoon
    • Proceedings of the PSK Conference
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    • pp.202.2-202
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    • 2003
  • A bioassay-guided fractionation of the whole extract of Viscum album (a parasitic plant : Loranthaceae) led to the isolation of two triterpenoidal components, oleanolic acid (1), ${\beta}$-amyrin acetate (2), homoflavoyadorinin B (3) as well as large quantity of free fatty acid mixtures as active ingredients of the extract responsible for the antitumoral property. The EtOAc soluble part and BuOH soluble part of the extract demonstrated a significant inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system) and HCT-15 (colon) in vitro, whereas the remaining water soluble part exhibited a poor inhibition. (omitted)

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Ethanol Extracts from Mistletoe (Viscum album L.) Act as Natural Antioxidants and Antimicrobial Agents in Uncooked Pork Patties during Refrigerated Storage

  • Kang, Suk-Nam
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.1
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    • pp.109-118
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    • 2016
  • The antioxidant potential of mistletoe (Viscum album L. var. coloratum Ohwi; VAL) extract in uncooked pork patties was evaluated. Three concentrations of VAL extract (0.1 [T1], 0.5% [T2] and 1.0% [T3]) along with 0.02% ascorbic acid as a positive control (V) were added to ground pork and pork patties were prepared. Incorporation of VAL extract decreased (p<0.05) the pH of the pork patties throughout the storage time and reduced (p<0.01) the thiobarbituric acid reactive substance values after day 14 of storage. Total plate counts of the VAL extract-treated samples and V-treated samples were also significantly lower (p<0.01) than that of the control (C) throughout the storage period. In addition, odor scores of the VAL extract-treated patties were lower than those of the C- or V-treated samples on 3rd day of the storage period. These results demonstrated that the VAL extract acts as a natural antioxidant in uncooked pork products.

Korean Mistletoe (Viscum album Coloratum) Extract Induces Eel (Anguilla japonica) Non-specific Immunity

  • Yoon, Taek-Joon;Park, Kwan-Ha;Choi, Sang-Hoon
    • IMMUNE NETWORK
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    • v.8 no.4
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    • pp.124-129
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    • 2008
  • Background: The immunomodulatory effects of Korean mistletoe (Viscum album Coloratum) on the innate immune responses of eel (Anguilla japonica) were studied. Methods: Mistletoe, Freund’s complete adjuvant (FCA), or phosphate-buffered saline (PBS) as a control was injected into eel peritoneal cavities. Results: Nitroblue tetrazolium (NBT)-positive cells in the head kidney of eel were significantly augmented by the second day post-injection of mistletoe. Reactive oxygen intermediates (ROI) were more produced in mistletoe-injected fish kidney leucocytes than in FCA-injected ones. The level of lysozyme activity in the serum of fish 2 days after injection with mistletoe was also significantly higher than that in the serum of the control fish. The optimal concentration of mistletoe in inducing the highest serum lysozyme activity was revealed to 500${\mu}$g/200 g of fish. In phagocytic activity assay, mistletoe-sensitized eel kidney phagocytes captured more zymosan than did the control fish. Conclusion: Korean mistletoe appeared to be a good activator of the non-specific immune responses of eel.

Inhibition of Porcine Pancreatic Elastase (PPE) by Korean Mistletoe (Viscum album var.coloratum) Fractions

  • Lyu, Su-Yun;Moon, You-Sun;Kwon, Young-Ju;Joo, Hye-Jin;Park, Won-Bong
    • Natural Product Sciences
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    • v.9 no.4
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    • pp.278-285
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    • 2003
  • The serine proteases such as human leukocyte elastase (HLE) and porcine pancreatic elastase (PPE) are classified in the chymotrypsin family, and possibly the most destructive enzymes having the ability to degrade virtually all of the connective components in the body. In the present study, the extracts of water and methanol of Korean mistletoe (Viscum album var. coloratum) inhibited significantly the PPE activity. The fractions eluated on Amberlite XAD-2 from methanol extract were further purified on the repeated $SiO_2$ column chromatography and the fractions A, B and C were eluated. The fractions A, B and C at 3 mg/ml inhibited significantly the PPE activity up to 66%, 95% and 85%, respectively. In conclusion, the fraction A assumed as lignans or phenylpropanes, and fraction B and C assumed as triterpenoids showed the PPE inhibitory effects on the PPE and that these compounds in mistletoe may be used for treatment of pathological processes such as age-dependent tissue loss or inflammation.

Korean Mistletoe, Viscum album coloratum Induces Non-Specific Immune Responses in Japanese Flounder, Paralichthys olivaceus

  • Choi, Sang-Hoon;Kim, Jong-Bae;Yoo, Yung-Choon;Yoon, Taek-Joon
    • Journal of Aquaculture
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    • v.17 no.3
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    • pp.209-214
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    • 2004
  • Effects of Korean mistletoe, Viscum album coloratum on the non-specific immune responses of Japanese flounder, Paralichthys olivaceus were examined. Flounder were inoculated with mistletoe, Freunds complete adjuvant (FCA), or phosphate-buffered saline (PBS) as a control into their peritoneal cavities. Reactive oxygen intermediate (ROI) products were more enhanced in mistletoe-injected fish kidney phagocytes than in FCA-injected ones. The level of lysozyme activity detected in the serum of fish 4 d after injection with mistletoe was also significantly higher than that found in the serum of the control fish. The appropriate concentration of mistletoe in eliciting the highest level of serum lysozyme activity was 500 $\mu$m/300 g of fish. In phagocytic activity assays, mistletoe-sen-sitized flounder kidney phagocytes captured more yeasts than those of the control fish. Korean mistletoe appeared to be a good activator of the non-specific immune responses of Japanese flounder.