• Title, Summary, Keyword: Viscum album

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An Antioxidant Homo-Flavoyadorinin-B from Korean Mistletoe (Viscum album var. coloratum) (한국산 겨우살이(Viscum album var. coloratum)로부터 분리한 homo-flavoyadorinin-B의 항산화 활성)

  • Choi, Seung-Young;Chung, Shin-Kyo;Kim, Suk-Kyung;Yoo, Yung-Choon;Lee, Kyung-Bok;Kim, Jong-Bae;Kim, Ja-Young;Song, Kyung-Sik
    • Applied Biological Chemistry
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    • v.47 no.2
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    • pp.279-282
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    • 2004
  • An antioxidant was isolated from Korean mistletoe (Viscum album var. coloratum) by consecutive purification using silica gel, Sephadex LH-20, and RP-HPLC. The active principle was identified as homo-flavoyadorinin-B (3',7-dimethoxyluteolin-4'-O-[apiosyl $(1{\rightarrow}2)$ glucoside]) by spectral analyses. It inhibited 74.6% of hydroxyl radical and 30.6% of superoxide anion radical at 0.01 mM; however, th~compound did not show any scavenging activity against hydrogen peroxide radical. At 0.1 mM, above compound scavenged superoxide anion radical about twice as effective as positive controls, BHT and ${\alpha}-tocopherol$. Radical scavenging activities of homo-flavoyadorinin-B on DPPH, hydroxyl, and hydrogen peroxide radicals were almost same with those of positive controls.

Chemical Pleurodesis Using Doxycycline and Viscum album Extract

  • Song, Kyung Sub;Keum, DongYoon;Kim, Jae Bum
    • Journal of Chest Surgery
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    • v.50 no.4
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    • pp.281-286
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    • 2017
  • Background: In chemical pleurodesis for managing pulmonary air leak, tetracycline derivatives are commonly used, and their effectiveness has been established in many studies. Recently, a Viscum album extract was used in chemical pleurodesis. We compared the effects of V. album with those of a tetracycline derivative (doxycycline) to demonstrate the therapeutic effectiveness of the V. album extract in chemical pleurodesis for managing pulmonary air leak. Methods: Between October 2010 and October 2016, chemical pleurodesis was performed using doxycycline in 40 patients and the V. album extract in 37 patients. Thirty-three patients were in the postoperative state after pulmonary resection, and 44 patients suffered from spontaneous pneumothorax. Results: No statistically significant difference in the success rate was observed between the 2 groups (V. album extract and doxycycline). In both groups, chest pain was the most common complication. More patients in the doxycycline group complained of severe chest pain (42.1% vs. 13.5%, p=0.006). In the V. album extract group, 24.3% of the patients required a chest tube to drain the pleural effusion after cessation of the air leak (doxycycline group: 5%, p=0.022). Further, the amount of pleural effusion drained on the day after the last chemical pleurodesis in the V. album extract group was greater than that in the doxycycline group ($162.2{\pm}170.2mL$ vs. $97.0{\pm}77.2mL$, p=0.032). All patients were discharged from the hospital without complications after pleural effusion drainage. Conclusion: Considering that treatment using the V. album extract was less painful, V. album might be a feasible option for chemical pleurodesis. However, pleural effusion should be monitored carefully when using V. album extract for treating patients suffering from air leak.

Molecular Characterization of the Recombinant A-chain of a Type II Ribosome-Inactivating Protein (RIP) from Viscum album coloratum and Structural Basis on its Ribosome-Inactivating Activity and the Sugar-binding Properties of the B-chain

  • Ye, Wenhui;Nanga, Ravi Prakash Reddy;Kang, Cong Bao;Song, Joo-Hye;Song, Seong-Kyu;Yoon, Ho-Sup
    • BMB Reports
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    • v.39 no.5
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    • pp.560-570
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    • 2006
  • Mistletoe (Viscum album) lectins, which are classified as a type II ribosome-inactivating protein (RIP) due to their unique biological function and the potential medical and therapeutic application in cancer cells, receive a rising attention. The heterodimeric glycoproteins contain the A-chain with catalytic activity and the B-chain with sugar binding properties. In recent years, studies involving the lectins from the white berry European mistletoe (Viscum album) and the yellow berry Korean mistletoe (Viscum album coloratum) have been described. However, the detailed mechanism in exerting unique cytotoxic effect on cancer cells still remains unclear. Here, we aim to understand and define the molecular basis and biological effects of the type II RIPs, through the studies of the recombinant Korean mistletoe lectin. To this end, we expressed, purified the recombinant Korean mistletoe lectin (rKML), and investigated its molecular characteristics in vitro, its cytotoxicity and ability to induce apoptotic cell death in cancer cells. To gain structural basis for its catalytic activity and sugar binding properties, we performed homology modeling studies based on the high degree of sequence identity and conserved secondary structure prediction between Korean and European, Himalayan mistletoe lectins, and Ricin.

Analysis of the Characterizing Compounds of Korean Mistletoes (Viscum album var. coloratum) (한국산 겨우살이의 지표성분 분석 연구(제 1 보) (기주식물과 생장지역에 따른 지표성분 변화 분석))

  • Choi, Kyung;Park, Kwang Woo;Hwang, Keum Hee
    • Korean Journal of Pharmacognosy
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    • v.44 no.2
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    • pp.138-148
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    • 2013
  • The three biochemical constituents pointed out as the bioactive principles of anticancer activity of Korean mistletoes, Viscum album var. colorantum, epiphyting to different host trees, different region, and different growth environments were compared. The characterizing compounds were lectin, which was glycoprotein reported has anticancer activity, homoflavoyadorinin-B, which was flavonoid glycoside reported as an antioxidant and oleanolic acid, as an anticancerous triterpenoid. We established the analysis methods for three characterizing compounds and determined by HPLC spectrophotometer, respectively. The average content of the lectin in V. album was 1.75 mg/g of dried plants and those of homoflavoyadorinin-B and oleanolic acid were 3.51 and 5.37 mg/g, respectively. The variations of contents of the three characterizing compounds according to epiphytic trees and growth region were observed but not found statistical significance clearly. We came to the conclusion that the contents of bioactive constituents of V. album except homoflavoyadorinin-B are not affected by epiphyting trees or growth environments.

Preparation of Alginate-Chitosan Microcapsules and Enteric Coated Granules of Mistletoe Lectin for Oral Administration

  • Lyu, Su-Yun;Moon, You-Sun;Kwon, Young-Ju;Park, Won-Bong
    • Proceedings of the PSK Conference
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    • pp.204.2-204
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    • 2003
  • The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cytotoxic and immune-system-stimulating activities. Korean mistletoe (Viscum album L. coloratum), a subspecies of European mistletoe, has also been reported to possess anticancer and immunological activities. A galactose- and N-acetyl-D-galactosamine- specific lectin (Viscum album L. coloratum agglutinin, VCA) with Mr 60 kDa was isolated from Korean mistletoe. (omitted)

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Triterpenes and Phenolic Constituents from Viscum album L. (상기생의 트라이테르펜 및 페놀성 성분)

  • 최상진;권학철;정애경;최상운;김경란;이선미;표석능;이강노
    • YAKHAK HOEJI
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    • v.45 no.6
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    • pp.591-598
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    • 2001
  • The photochemical study of Viscum album (Loranthaceae) led to the isolation of twelve compounds, lupeol (1), betulonic acid (2), betulinic acid (3), terminic acid (4), ursolic acid (5), $\beta$-sitosterol (6), $\alpha$- spinasterol (7), oleanolic acid (8) , 5-hydroxy-1- (4′-hydoxyphenyl) -7- (4"-hydroxyphenyl) -hepta-1- en-3-on (9), 2′-hydroxy -4′, 6′- dimethoxychalcone -4-O-glycoside (10) ,2′-hydroxy-4′, 6′-dimethoxychalcone -4-O- [apiosal(1longrightarrow12)]glucoside (11) and syringin (12). Their structures were established by chemical and spectroscopic methods. The cytotoxicity of the isolated compounds was evaluated by sulforhodamine B assay against five cultured human tumor cell lines.

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Preliminary Toxicity and General Pharmacology of KML-IIU, a Purified pectin from Korean Mistletoe (Viscum album coloratum) (한국산 겨우살이 (Viscum album coloratum)로부터 정제된 렉틴 성분 KML-IIU의 예비 독성 및 일반 약리 시험)

  • 강태봉;윤택준;김종배;송성규;이관희
    • YAKHAK HOEJI
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    • v.45 no.3
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    • pp.251-257
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    • 2001
  • The study was carried out to evaluate the preliminary toxicity and general pharmacology of KML-IIU, a purified pectin from Korean Mistletoe (Viscum album coloratum). KML-IIU was administered intravenously to ICR mice and Spargue-Dawley rats to investigate the acute toxicity. LD50 values in mice and rats were above 30 $\mu$g/kg. KML-IIU had no effects on the general behaviors, acetic acid induced writhing syndrome, pentobarbial induced sleeping time, pentylenetetrazole induced convulsion and the change of body temperature. In addition, KML-IIU did not show any effects on digestive system and blood coagulation system.

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Immunodulatory Activity of Triterpenes and Phenolic Compounds from Viscum Album L. (상기생의 트라이테르펜 및 페놀성 성분의 면역조절 작용)

  • 박대섭;최상진;김경란;이선미;이강노;표석능
    • Biomolecules & Therapeutics
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    • v.11 no.1
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    • pp.1-4
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    • 2003
  • Plants are known as important source in the search for new drugs. The twelve compounds from Viscum album (Loranthaceae), lupeol (1), betulonic acid (2), betulinic acid (3), terminic acid (4), ursolic acid (5), $\beta$-sitosterol (6), $\alpha$-spinasterol (7), oleanolic acid (8), 5-hydroxy-1-(4′-hydoxyphenyl)-7-(4"-hydroxyphenyl)-hepta-1-en-3-on (9), 2′-hydroxy-4′,6′-dimethoxychalcone-4-O-glucoside (10), 2′-hydroxy-4′,6′-dimethoxychalcone-4-O-[apiosyl(l$\longrightarrow$2)]glucoside (11) and syringin (12) were evaluated for their immunomodulatory properties. Compounds 6 and 11 induced the macrophage tumoricidal activity and the lymphocyte blastogenesis. In addition, these compounds stimulated the macrophages to induce the production of TNF-$\alpha$ and NO. These findings suggest that compounds 6 and 11 are modulating various elements of the host immune response.

Taxonomic identity of the plant name Sanggisaeng (식물명 상기생(桑寄生)의 분류학적 실체)

  • SHIN, Hyunchur;JUNG, Jongduk
    • Korean Journal of Plant Taxonomy
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    • v.48 no.4
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    • pp.357-362
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    • 2018
  • References in the modern Korean literature in this area to the Korean medicinal plant name written in Chinese script and expressed as Sanggisaeng have been extremely confusing. This name has been considered as six species, specifically Loranthus parasiticus, Loranthus tanakae, Taxillus yadoriki, Taxillus chinensis, Korthalsella japonica, and Viscum album var. coloratum. Recently, it was suggested that sanggisaeng is not distributed in Korea; however, the plant name Sanggisaeng recorded in old Korean literature sources, such as in the Hyang-yak-chae-chwi-wol-lyeong, Hyang-yak-jib-seong-bang, and Dong-ui-bo-gam sources, among others, was identified using the name V. album var. coloratum, whereas in China, Sanggisaeng was referred to as T. sutchuenensis. The Hangeul name of Sanggisaeng had been Ppongnamugyeousali, but now the name Gyeousali is widely used.

Isolation and Characterization of $\beta$-Galactoside Specific Lectin from Korean Mistletoe (Viscum album var. coloratum with Lactose-BSA-Sepharose 4B and Changes of Lectin Conformation

  • Park, Won-Bong;Ju, Yeun-Jin;Han, Seon-Kyu
    • Archives of Pharmacal Research
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    • v.21 no.4
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    • pp.429-435
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    • 1998
  • Lectins and its A- and B-chains from Korean mistletoe (Viscum album var. coloratum) were isolated by affinity chromatography on the Sepharose 4B modified by lactose-BSA conjugate synthesized by reductive amination of ligand (lactose) to .epsilon.-amino groups of lysine residues of spacer (BSA) after reduction by $NaCNBH_3$. The lactose-BSA conjugate was coupled to Sepharose 4B activated by cyanogen bromide. The molecular weight determined by SDS-PAGE were a 31 kD of A-chain and a 35kD of B-chain. Amino acid analysis and N-terminal sequencing were performed. The effects of pH, temperature and guanidine chloride on the conformation of the lectin were investigated by measuring its intrinsic fluorescence and compared with its hemagglutinating activities. Blue shift was detected on the acidic pH and there was a close relationship between activities and conformation of the lectin. Under denaturing conditions, the tryptophan emission profile of lectin showed typical denaturaiional red shift which also correspond to the conformations and activity of lectin.

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