• Title/Summary/Keyword: Vibrio vulnificus

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Contamination of Shellfish with Vibrio vulnificus the Present Situation and Countermeasures (어패류의 비브리오패혈증 균 오염과 그 대책)

  • 김영만
    • Journal of Food Hygiene and Safety
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    • v.8 no.2
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    • pp.13-21
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    • 1993
  • Vibrio septicemia,, resulting in high mortality, has been caused by Vibrio vulnificus. Ingestion of marine products or contact with sea water contaminated with Vibrio vulnificus can cause septicemia. Vibrio vulnificus has been detected world wide and west sea area of Korea, Kum river estuary in particular, showed high detection rate. Higher detection rate of Vibrio vulnificus were reported in the bottom deposit with low depth of water, low salinity, and high COD. Man with the liver disease can easily come down with Vibrio septicemia and the main source is the sliced raw fish dish. The preventive measure for this disease is to wash raw fish material thoroughly with tap water and handle in sanitary conditions. Washing with sea water is strictly prohibited . It may be necessary to forbid the small-scale businessmen from selling the sliced ray fish dish in the vicinity of seashore. Man with the liver disease of diabetes should not swim or consume the raw fish dish in the contaminated area during summer.

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Mechanism of Vibrio vulnificus Cytolysin on Rat Platelet Aggregation (Vibrio vulnificus cytolysin의 흰쥐 혈소판 응집 기전)

  • 김현철;채수완;이병창;은재순
    • YAKHAK HOEJI
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    • v.43 no.6
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    • pp.802-808
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    • 1999
  • Vibrio vulnificus cytolysin has been incriminated as one of the important virulence determinants in V. vulnificus infection. In the present study, the effects of Vibrio vulnificus cytolysin on platelets were examined. Vibrio vulnificus cytolysin induced platelet aggregation and increased intracellular calcium concentration ($[Ca^{2+}]_i$) of rat platelets. These effects were abolished in $Ca^{2+}-free$ buffer (2 mM EGTA). Cytolysin also potentiated ADP-and collagen-induced platelet aggregation. Lanthanum (2 mM) inhibited cytolysin-diduced platelet aggregation. However, another $Ca^{2+}$ channel blockers, verapamil ($20{\;}{\mu}M$) or mefenamic acid ($20{\;}{\mu}M$) did not block cytolysin-induced platelet aggregation. Osmotic protectants, sucrose (50 mM) and raffinose (50 nM) suppressed platelet aggregation by 35.9% and 63.4%, respectively. V. vulnificus cytolysin increased membrane conductances of platelet membranes. These results suggest that cytolysin-induced platelet aggregation is mediated via lanthanum sensitive-calcium influx which resulted from the pore formation by V. vulnificus cytolysin.

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Purification and Characterization of Endotoxin from Vibrio vulnificus (비브리오 패혈증균의 균체내독소 정제 및 특성에 관하여)

  • 김영만;정현정;신일식
    • Journal of Life Science
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    • v.7 no.2
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    • pp.79-87
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    • 1997
  • To determine the cause of Vibrio septicemia by understanding the characteristics endotoxin from Vibrio vulnificus, lethal dose, heat resistance and vascular permeability enhancing activity were svaluated using vegestative cell and cell homogenate and the result is as follows: 1. Vibrio vulnificus CDC B3547 of patient origin did not exihibit any significant difference in toxicity compared to Vibrio vulnificus B57 of enviroment origin. 2. Strong toxicity was observed when viable cell count of Vibrio vulnificus CDC B3547 was more than 10$^{7}$/ml. 3. Toxicity of cell homogenate was completely inactivited upon geating at 80$^{\circ}$C for 20min. 4. Cell homogenate did not show hemolyic activity but was acknowleged to have cytotoxicity. 5. Major lethal toxin against mouse was existed in Vibrio vulnificus CDC B3547; however, separation of LPS and LPS-protein complex was not successful using the current technique.

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Lethal Toxin from Vibrio vulnificus (Vibrio vulnificus의 치사독성에 관하여)

  • Kim, Young-Man
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.2
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    • pp.175-180
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    • 1989
  • Toxins from Vibrio vulnificus cause Vibrio septicemia. Study was carried out for localization, characterization and toxicity of these toxins by injection thorough introspectional route to ICR(Insititude cancer research) mouse using Vibrio vulnificus M -1 isolated from patient and Vibrio vulnificus S-1 from sea water. No significant differences in lethal toxicity were observed between Vibrio vulnificus M-1 and Vibrio vulnificus $S-1.\;LD_{50}$ was $7.80{\times}10^6$ cells when these bacteria were injected to ICR mouse thorough intraperitoneal route. Crude hemolysin from Vibrio vulnificus S-1 did not show lethal toxiity and this lethal toxin were found to be endotoxin. This endotoxin were completely inactivated upon incubation at $80^{\circ}C$ for 20min.

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The Optimal Culture Condition for the Collagenolytic Protease Production from Vibrio vulnificus CYK279H (Vibrio vulnificus CYK 279H에서 Collagenolytic Protease 생산을 위한 최적배양조건)

  • 강성일;김영문;장영부;임동중;공재열
    • KSBB Journal
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    • v.19 no.4
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    • pp.295-300
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    • 2004
  • A marine bacterium for producing an collagenolytic protease was isolated from the southern sea of Korea and identified as Vibrio vulnificus and named as Vibrio vulnificus CYK279H. This strain producing an collagenolytic protease was showed high activity toward collagen and gelatin as substrate. The optimum initial pH, NaCl, and temperature for cell growth and protease production was 7.5, 2.0% and 25$^{\circ}C$, respectively. Optimization for collagenolytic protease production was composed of 0.3% D-galactose, 0.6% yeast extract, 4.0% gelatin, 0.2% (NH$_4$)$_2$SO$_4$, and 0.2 mM ferric citrate in artificial sea water. The maximum protease production was required gelatin and yeast extract. The collagenolytic protease production by Vibrio vulnificus CYK279H reached a maximum of 73 unit/l after the cultivation for 18 h under the optimized medium.

Distribution of Vibrio vulnificus in Chonnam Coastal Area (전만해안지역의 비브리오 패혈증균(Vibrio vulnificus) 분포)

  • Yang, Ho-Chul;Hong, Suk-Soon;Kim, Kai-Hoan;Choi, Sang-Ho;Chung, Hee-Jong
    • Microbiology and Biotechnology Letters
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    • v.27 no.1
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    • pp.70-74
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    • 1999
  • Vibrio vulnificus, a normal bacterial inhabitant of estuaries, is of concern because it can be a potent human pathogen, a causing septicemia, wound infections and gastrointestinal disease in susceptible host. In this survey, total 431 samples were obtained from different sites of the Chonman coastal area during the periods from Mar. 1997 to Feb. 1998. Vibrio vulnificus was isolated from the middle of May to the begining of November of 1997 in Chonman coastal area, as the seawater temperature was at 20$^{\circ}C$ and 15$^{\circ}C$, respectively and was rapidly increased to above 40% from July to September. The isolation rates of V. vulnificus from sediment, seawater, raw seafoods and aquarium water were 52.1%, 49.1%, 32.5%, and 27.3%, respectively and isolation rate was highest in oyster among various collected samples. V.vulnificus was also isolated from 73.1%(38/52) of sampling sites of Chonman coastal area.

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disinfectant and Inhibitory Effect of Natural Antimicrobial Agent on Vibrio vulnificus in Fish (천연항균성 물질을 이용한 Vibrio vulnificus 의 살균 및 독소생성 억제효과)

  • 조성환;서일원;최종덕;전상수;라택균;정수근;강동훈
    • Journal of Food Hygiene and Safety
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    • v.7 no.2
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    • pp.99-106
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    • 1992
  • To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50∼100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonica GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnifiucs the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight, protein efficiency ratio, serum enzymes and serum blood components of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself an fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

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Preparation of Antiserum against Hemolysin from Vibrio vulnificus using Hemolysin-bound Liposomes (Liposome을 이용한 Vibrio vulnificus가 생산하는 용혈독소의 항혈청 제조법)

  • 김영만
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.1
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    • pp.91-95
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    • 1993
  • To investigate hemolysin from Vibrio vulnificus in terms of protein chemistry and immunochemistvy, the simple method to produce antiserum was developed as follows ; Crude hemolysin from Vibrio vulnificus was mixed with cholesterol-phosphatidylcholine-liposome. Only hemolysin with molecular weight of 50kD was selertively bound to the liposome. Thus, without purification of crude hemolysin, liposome bound hemolysin was used as antigen to produce antiserum by injecting into back muscle of a rabbit. Resultant antiserum reacted only with hemolysin. Hemouysin of Vibrio vulnificus from patients and environment was formed single band in gel diffusion precipitation reaction with antiserum.

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AN EVIDENCE FOR THE INVOLVEMENT OF CYTOLYSIN IN VIBRIO VULNIFICUS DISEASE

  • Park, Moon-Kook
    • Toxicological Research
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    • v.4 no.2
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    • pp.143-149
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    • 1988
  • Cytolysin produced by Vibrio vulnificus ATCC 27562 was partially purified by sequential ammonium sulfate precipitation, gel filtration with Sephadex G-200, and ion exchange chromatography with DEAE-Sephadex. The partially purified cytolysin was inactivated by cholesterol. More than one molecule of the cytolysin was required to lyse a single erythrocyts. The antiserum against cytolysin enhanced the survival ratio of mice infected with low dose of V. vulnificus.

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Comparison of the immunogenicity between bacterial ghost and formalin-killed bacteria for Vibrio vulnificus

  • Kwon, Se Ryun
    • Journal of fish pathology
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    • v.25 no.3
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    • pp.159-164
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    • 2012
  • Vibrio vulnificus ghosts (VVG) were generated using a mobilizable vector including a thermosensitive expression cassette by conjugation. The vaccine potential of VVG was investigated in mouse. Mice immunized with VVG showed significantly higher antibody titer than those with formalin-killed V. vulnificus. The present study supports the conceptive usefulness of bacterial ghosts as vaccine candidates.