• Title, Summary, Keyword: Vibrio alginolyticus

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Differentiation of Vibrio spp. including Core Group Species by PCR-RFLP (PCR-RFLP에 의한 Vibrio core group을 포함한 Vibrio 종의 구분)

  • Park, Jin-Sook
    • Journal of Life Science
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    • v.22 no.2
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    • pp.245-250
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    • 2012
  • The 16S rDNA - RFLP types for six Vibrio species (V. fluvialis, V. proteolyticus, V. vulnificus, V. mimicus) including two core group members, V. alginolyticus and V. parahaemolyticu s, and Grimontia (Vibrio) hollisae were determined using PCR-RFLP analysis. Six tetrameric restriction enzymes (Alu I, Cfo I, Dde I, Hae III, Msp I, and Rsa I) were selected for RFLP analysis. V. alginolyticus, V. parahaemolyticus, and V. proteolyticus showed the same RFLP pattern following digestion with four of the six used restriction enzymes: CfoI, DdeI, MspI, and RsaI. Various restriction enzyme combinations generated digests recognizable as distinct RFLP types for each of the assayed Vibrio species. In particular, AluI single digestion produced species specific band patterns that enabled the differentiation between these Vibrio species. Dendrogram based on restriction patterns showed that two Vibrio core group members, V. alginolyticus and V. parahaemolyticus were closely related having a similarity over 90%. Although the observed RFLP pattern for Grimontia hollisae shared several common bands with other Vibrio spp., G. hollisae results were still clearly distinct from Vibrio spp. RFLP types for all restriction enzymes tested. If restriction enzymes are aptly selected, PCR-RFLP analysis is still a rapid and effective tool for differentiating Vibrio species.

Biological Characterization of a Vibrio alginolyticus-Specific Bacteriophage (Vibrio alginolyticus에 대한 특이 bacteriophage의 생물학적 특성)

  • Heo, Yong Ju;Lee, Chan Heun;Baek, Min Suk;Ahn, Hyun Mi;Hwang, Yo Sep;Park, Kwon-Sam;Choi, Sanghoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.6
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    • pp.654-658
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    • 2012
  • Vibrio alginolyticus, a marine fish and shellfish pathogen, has been found at a high frequency around the coastal areas of Korea. Vibrio alginolyticus was purified from various diseased fish, and a V. alginolyticus-specific bacteriophage was isolated from seawater obtained from fish farms located on the west coast of Korea. In a bacterial lysis experiment using the phage and antibiotics, tetracycline, $10^3$ cfu/ml of V. alginolyticus were completely lysed by both the phage and the antibiotic, suggesting that the purified phage in the study could be utilized as an alternative to antibiotics in the control of fish and shellfish diseases caused by V. alginolyticus.

Antimicrobial Susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from Fish Farms on the Southern Coast of Korea (남해안 어류양식장에서 분리된 Vibrio parahaemolyticus와 Vibrio aiginolyticus의 항균제 감수성)

  • SON Kwang-Tae;OH Eun-Gyoung;LEE Tae-Seek;LEE Hee-Jung;Kim Poong-Ho;KIM Ji-Hoe
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.6
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    • pp.365-371
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    • 2005
  • The antimicrobial resistance patterns to the 10 antimicrobial agents of potential pathogenic vibrios isolated from seawater and a variety of farmed fishes, including oliver flounder (Payalichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagyus major) and sea bass (Lateolabyax japonicus), were investigated from May to October, 2004. A total of 314 strains of the genus vibrios were isolated from 126 collected samples, and the number of isolated strains of Vibrio payahaemolyticus and Vibrio alginolyticus were 194 and 120, respectively. Apparently $98.5\%$ of V. parahaemolyticus and $100\%$ of V. alginolyticus isolates demonstrated antimicrobial resistance against at least one antimicrobial agent. The resistance of V. parahaernolyticus isolates to ampicillin ($97.9\%$) was highest, followed by oxolinic acid ($26.8\%$), amikacin ($19.1\%$) and tetracycline and amoxicillin/clavulanic acid ($6.7\%$). V. alginolyticus isolates were resistant to ampicillin ($100\%$), solfamethoxazoleit,imethopenem ($25\%$), amikacln ($21.7\%$), amoxicillin/clavulanic acid ($15.8\%$), ciprofloxacin ($13.3\%$), and tetracycline and doxycycline ($11.7\%$). The rate of multiple antimicrobial resistance to at least four antimicrobials was higher in the V. alginolyticus isolates ($20.8\%$) than in the V. parahaemolyticus ($6.7\%$).

Morphological characterization of Vibrio alginolyticus specific bacteriophage isolated from fish farms on west coast of Korea (서해안 양식장에서 분리한 Vibrio alginolyticus의 특이 bacteriophage에 대한 구조적 특성)

  • Heo, Yong Ju;Lee, Chan Heun;Baek, Min Suk;Ahn, Hyun Mi;Hwang, Yo Sep;Park, Kwan Ha;Choi, Sang Hoon
    • Journal of fish pathology
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    • v.25 no.3
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    • pp.165-172
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    • 2012
  • Vibrio alginolyticus (V. alginolyticus), which is one of bacterial pathogens evoking severe infection in fish and shellfish as well as in human has been found at high frequency around all coast areas in Korea. Both V. alginolyticus and V. alginolyticus specific bacteriophage were isolated from sea water and various fishes from fish farms on west coast in Korea. In a morphological study based on electron microscope, the purified phage appeared to be composed of hexagon head of 60 nm and short tail of 20 nm. In the denatured SDS-PAGE analysis, the structural proteins of the phage were found to be 7 different protein fractions ranging from 37.8 to 198 kda. The kind of nucleic acid of the phage was ascertained to a double stranded DNA.

The Biological Characteristics and Drug Resistance of Vibrio Species (Vibrio균속의 생물학적 성상 및 약제내성에 관하여)

  • Park, Chul-Hee;Lee, Yun-Tai
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.4
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    • pp.413-425
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    • 1987
  • In the present experiments, isolated Vibrio species from marine and clinical specimens from July, 1985 to October, 1986, had the results as follows: 1. The 55 strains of Vibrio were isolated and identified; Vibrio parahaemolyticus was 35 strains, Vibrio vulnificus was 10 strains, Vibrio alginolyticus was 10 strains. 2. In the K-serotyping of Vibrio parahaemolyticus, fourteen serotypes identified but three were not strains typable by the availble K-antisera. 3. In the Kanagawa phenomenon experiment of Vibrio parahaemolyticus, it proved positive reaction, 14 of 15 strains(93%) isolated from the patient and 13 of 20 strains(65%) isolated from the nature. 4. In twelve antibiotic resistance experiments, Vibrio parahaemolyticus and Vibrio alginolyticus showed 100% resistance on ampicilline, but Vibrio vulnificus showed 100% sensitivity. But all of them proved 100% sensitivity on chloramphenicol, tetracycline, nalidixic acid. 5. In the antibiotic resistance patterns, Vibrio parahaemolyticus proved that 15 strains(43%) resisted on 4 antibiotics and 5 strains(14%) resisted on 7 antibiotisc and. Vibrio vulnificus proved that 1 strain(10%) resisted on 2 antibiotics and 6 strains(60%) without resistance, Vibrio alginolyticus proved that 7 strains(70%) resisted on 3 antibiotics and 2 strains(20%) resisted on 8 antibiotics.

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Properties of an Extracellular Amylase Produced by the Marine Halophilic Bacterium Vibrio alginolyticus (해양 호염성 세균 Vibrio alginolyticus가 생산하는 Extracellular Amylase의 특성)

  • 김영재
    • Microbiology and Biotechnology Letters
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    • v.27 no.3
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    • pp.203-207
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    • 1999
  • V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45$^{\circ}C$. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.

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DISTRIBUTION OF VIBRIO PARAHAEMOLYTICUS AND V. ALGINOLYTICUS IN THE COAST OF CHUNG-MU (충무연안의 Vibrio parahaemolyticus와 V. alginolyticus의 분포)

  • LEE Won-Jae;AHN Cheol-Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.4
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    • pp.233-237
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    • 1976
  • This study was carried out to evaluate the distribution of Vibrio parahaemolyticus and Vibrio alginolyticus in sea water, mud, oyster (Crassostrea gigas) and sea mussel (Mytilus edulis) collected from the coast of Chung-mu during the period from July 1975 to September 1976. Fifty one strains of V. parahaemolyticus and 160 strains of V.. alginolyticus were isolated from 420 samples. The distribution varied by month showing the highest in July through September The isolation ratio of V. parahaemolyticus was $28\%$ for mud, $24\%$ for sea water, $5\%$ for sea mussel and $4.2\%$ for oyster. The morphological, physiological and biochemical characteristics of 211 isolated strains were coincided with those of the typical V. parahaemolyticus and V. alginolyticus.

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Vibrio alginolyticus MviN is a LuxO-regulated Protein and Affects Cytotoxicity Towards Epithelioma Papulosum Cyprini (EPC) Cells

  • Cao, Xiaodan;Wang, Qiyao;Liu, Qin;Liu, Huan;He, Honghong;Zhang, Yuanxing
    • Journal of Microbiology and Biotechnology
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    • v.20 no.2
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    • pp.271-280
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    • 2010
  • Vibrio alginolyticus, a Gram-negative marine bacterium, is one of the causative agents of fish vibriosis. Its virulence factors and pathogenesis mechanism are barely known, except for some extracellular products (ECPs) that are known to be regulated by quorum sensing system. Therefore, the present study used a microarray to analyze the transcription profiles of the wild-type V. alginolyticus and a deletion mutant of luxO, the pivotal regulator in Vibrio quorum sensing systems, which resulted in the identification of a putative virulence factor, MviN. Quantitative real-time reverse transcription PCR confirmed that the transcription of mviN was upregulated in the luxO mutant when compared with wild-type, and down regulated in a luxO-con complemented strain. Furthermore, Western blotting indicated that MviN was greatly induced during the late-exponential and stationary phases of growth, indicating that the expression of MviN was cell-density dependent and quorum sensing regulated in V. alginolyticus. Meanwhile, the mviN null mutant displayed a much slower growth rate than the wild type, signifying the essential role of MviN in V. alginolyticus. Western blotting also revealed that MviN was present as an extracellular protein in V. alginolyticus. When epithelioma papulosum cyprini (EPC) cells were treated with the ECPs of the mviN mutant, no cytotoxicity was observed, whereas EPC cells treated with the wild type exhibited pathological changes, which increased with the ECPs concentration and treatment time. Therefore, the results demonstrated that MviN is a LuxO-regulated ECPs component and involved in the pathogenicity of V. alginolyticus.

Optimization of diesel biodegradation by Vibrio alginolyticus using Box-Behnken design

  • Imron, Muhammad Fauzul;Titah, Harmin Sulistiyaning
    • Environmental Engineering Research
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    • v.23 no.4
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    • pp.374-382
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    • 2018
  • Petroleum hydrocarbons pollutants, such as diesel fuel, have caused ecosystem damage in terrestrial and aquatic habitats. They have been recognized as one of the most hazardous wastes. This study was designed to optimize the effect of Tween 80 concentration, nitrogen (N)/phosphorus (P) ratio and salinity level on diesel biodegradation by Vibrio alginolyticus (V. alginolyticus). Response surface methodology with Box-Behnken design was selected with three factors of Tween 80 concentration (0, 5, 10 mg/L), N/P ratio (5, 10, 15) and salinity level (15‰, 17.5‰, 20‰) as independent variables. The percentage of diesel degradation was a dependent variable for 14 d of the remediation period. The results showed that the percentages of diesel degradation generally increased with an increase in the amount of Tween 80 concentration, N/P ratio and salinity level, respectively. The optimization condition for diesel degradation by V. alginolyticus occurred at 9.33 mg/L of Tween 80, 9.04 of N/P ratio and 19.47‰ of salinity level, respectively, with percentages of diesel degradation at 98.20%. The statistical analyses of the experimental results and model predictions ($R^2=0.9936$) showed the reliability of the regression model and indicated that the addition of biostimulant can enhance the percentage of diesel biodegradation.

Effect of Vibrio alginolyticus on the Algicidal Activity of Shewanella sp. SR-14 (Vibrio alginolyticus가 Shewanella sp. SR-14의 미세조류 증식저해 활성에 미치는 영향)

  • KIM Ji Hoe;PARK Hee Yeon;LEE Tae Seek;KIM Shin-Hee;PARK Jeong Heum;CHANG Dong Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.430-434
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    • 2001
  • The algicidal activity of Shewanella (formerly Alteromonas) sp. SR-14 against diatom, Chaetoceros calcitrans was reported in our previous papers. In this study, the effect of Vibrio alginolyticus on the algicidal activity of Shewanelia sp, SR-14 was examined under the optimum algicidal conditions, i.e., temperature ($21\pm1^{\circ}C$), light intensity (4,000 lux), and light: dark cycle (12 hour: 12 hour). Shewanella sp. SR-14 grew well in the presence or the absence of V. alginolyticus in Conwy medium. Algal growth was only inhibited by Shewanella sp. SR-14. V. alginolyticus did not show the algicidal activity, Growth of C. calcitrans increased synergistically with growth of V. alginolyticus. When the initial inoculum of V. alginolyticus was only 1 log cycle higher than that of Shewanella sp. SR-14, the effect of V. alginolyticus on the algicidal activity of Shewanella sp. SR-14 was insignificant during incubation of mixed culture, i.e., two bacterial species and the alga. However, when V. alginolyticus dominated Shewanella sp. SR-14 by 3 log cycles of bacterial counts, it was found that the strain SR-14 could not inhibited growth of C. calcitrans up to 5 days of incubation.

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