• Title, Summary, Keyword: Valine

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Effect of Valine on facilitated O1e1in Transport Membranes (올레핀 촉진수송 분리막의 성능향상을 위한 Valine의 효과)

  • Yong Soo Kang;Sang Wook Kang;Jong Hak Kim;Jongok Won;Kookheon Char
    • Membrane Journal
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    • v.13 no.2
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    • pp.125-129
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    • 2003
  • A remarkable separation performance of olefin/paraffin mixtures has been observed through facilitated olefin transport membranes consisting of silver ions dissolved in polymer matrices. In this research, valine, an amino acid, was introduced in poly (2-ethyl-2-oxazoline) (POZ)/$AgBF_4$ membranes to increase the separation performance. FT-IR spectra show that the cationic sites ($-NH_3^+$) of valine interact with the counter anion of the silver salt, resulting in the enhanced activity of the silver ions and consequently improved separation perfornance. Therefore, the $POZ/AgBF_4$ membranes containing valines exhibit the higher permeance as well as the higher selectivity of propylene/propane than those without valine.

Determination of Valine requirements by usging plasma free amino acid concentrations in rainbow trout(Oncorhynchus mykiss) with dorsal aorta cannulation

  • Sungchul C. Bai;Park, Gunjun;Im ho Ok;Kim, Youngchul
    • Proceedings of the Korean Aquaculture Society Conference
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    • pp.67-67
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    • 2003
  • Dorsal aorta cannulated rainbow trout averaging 505 $\pm$ 6.5g (Mean $\pm$ SD) were divided into 7 groups (5 fish per group). Twenty four hour post feeding, cannulated fish were intubated with one of seven L-amino acid diets containing graded levels of Valine (Val. : 0.45, 0.95, 1.20, 1.45, 1.70, 1.95 or 2.45%) at 1% of body weight. Blood samples were taken at 0, 5 and 24hr after feeding the experimental diets. Post-prandial (5h after feeding) plasma-free Valine concentrations (PPval.) increased linearly from fish fed diets containing Valine between 0.45 and 1.45%, but the concentration remained constant from fish fed diets containing valine between 1.45 and 2.45%. Post-absorptive (24h after feeding) plasma free valine concentrations (PAval) increased linearly from fish fed diets containing valine between 0.45 and 1.45%, but the concentration remained constant from fish fed diets containing valine between 1.45 and 1.95%. Using the broken-line model analysis, the dietary valine requirement by PPval and PAval could be 1.44 and 1.50% in rainbow trout, respectively. These results supported that the estimated dietary valine requirement by PP(val.) and PA(val.) could be in close agreement with the values obtained from the previous conventional feeding method. Thus, the use of PFAA concentrations for determining essential amino acid requirements could be possible in rainbow trout with dorsal aorta cannulation.

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Polymorphism of Growth Hormone GH1-AluI in Jersey Cows and Its Effect on Milk Yield and Composition

  • Dario, C.;Carnicella, D.;Ciotola, F.;Peretti, V.;Bufano, G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.1
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    • pp.1-5
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    • 2008
  • To analyze genetic effects on milk production traits, 164 unrelated Italian Jersey cows reared in 15 farms located in Southern Italy were characterized at the growth hormone locus. Using a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism protocol all three possible genotypes for the Leucine/Valine polymorphism were identified even if in the considered population the number of homozygous animals (Leucine/Leucine = 36; Valine/Valine = 28) was smaller than Leucine/Valine (n = 100) cows; the Hardy-Weinberg equilibrium was verified. Statistical analysis showed that daily milk yield in the Leucine/Leucine genotype was higher (p<0.01) than in Leucine/Valine (22.76 kg vs. 16.96 kg ); cows with Leucine/Valine genotype differed significantly from Leucine/Leucine and Valine/Valine genotypes particularly in fat (4.95% vs. 4.13% and 4.82%, respectively) and protein content (4.00% vs. 3.47% and 3.79%, respectively). No significant effect on lactation length was observed.

Effects of dietary valine:lysine ratio on the performance, amino acid composition of tissues and mRNA expression of genes involved in branched-chain amino acid metabolism of weaned piglets

  • Xu, Ye Tong;Ma, Xiao Kang;Wang, Chun Lin;Yuan, Ming Feng;Piao, Xiang Shu
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.1
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    • pp.106-115
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    • 2018
  • Objective: The goal of this study was to investigate the effects of dietary standard ileal digestible (SID) valine:lysine ratios on performance, intestinal morphology, amino acids of liver and muscle, plasma indices and mRNA expression of branched-chain amino acid (BCAA) metabolism enzymes. Methods: A total of 144 crossbred pigs (Duroc${\times}$Landrace${\times}$Large White) weaned at $28{\pm}4days$ of age ($8.79{\pm}0.02kg$ body weight) were randomly allotted to 1 of 4 diets formulated to provide SID valine:lysine ratios of 50%, 60%, 70%, or 80%. Each diet was fed to 6 pens of pigs with 6 pigs per pen (3 gilts and 3 barrows) for 28 days. Results: Average daily gain increased quadratically (p<0.05), the villous height of the duodenum, jejunum and ileum increased linearly (p<0.05) as the SID valine:lysine ratio increased. The concentrations of plasma ${\alpha}-keto$ isovaleric and valine increased linearly (p<0.05), plasma aspartate, asparagine and cysteine decreased (p<0.05) as the SID valine:lysine ratio increased. An increase in SID lysine:valine levels increased mRNA expression levels of mitochondrial BCAA transaminase and branched-chain ${\alpha}-keto$ acid dehydrogenase in the longissimus dorsi muscle (p<0.05). Conclusion: Using a quadratic model, a SID valine:lysine ratio of 68% was shown to maximize the growth of weaned pigs which is slightly higher than the level recommended by the National Research Council.

Partition of Amino Acid Requirements of Broilers between Maintenance and Growth. V. Isoleucine and Valine

  • Bae, S.H.;Kim, J.H.;Shin, I.S.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.3
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    • pp.388-394
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    • 1999
  • Two experiments were conducted to subdivide isoleucine (exp. 1) and valine (exp. 2) requirements for maintenance from the requirements for growth of broilers aged 1 to 3 weeks. Purified diets were used, containing five graded levels of isoleucine and valine. Based on weight gain response, the isoleucine requirement for growth was 7.50 mg/g weight gain and the daily isoleucine need for maintenance (mg) was 0.044 per unit metabolic body size ($(Wg^{0.75})$). Based on the N gain response, the isoleucine requirement for growth was 0.317 mg/mg N gain and the daily isoleucine need for maintenance (mg) was 0.040 per unit metabolic body size $(Wg^{0.75})$. Based on weight gain and N gain response, the total isoleucine requirement was calculated 244 mg/day or 0.59% of the diet, 274 mg/day or 0.66% of the diet, respectively. From the relationship of weight gain and N gain, 5.07% of the retained protein was comprised of isoleucine; the reported isoleucine content of chick muscle was 4.42%. The valine requirement for growth was 9.84 mg/g weight gain and 0.36 mg/mg N gain whereas the maintenance requirement was 0.046 or 0.052 mg per unit of metabolic body size (Wgo.11. According to the model developed to estimate valine requirement, the total requirement was 319 mg/day or 0.77% of the diet, 315 mg/day or 0.76% of the diet, respectively. Previous reported valine requirements for growing chicks of 7~24 days old were in close agreement with these estimates. As a percentage of retained protein, valine was calculated to be 5.81% ; the reported valine concentration of crude protein of chicks' body including feathers was 6.72%.

Studies on the Modification of Polyacrylonitrile(VI) -Copolymerization of Acrylonitrile with N-methacryloyl-DL-$\alpha$-valine- (Polyacrylonitrile의 개질에 관한 연구(VI) -Acrylonitrile과 N-methacryloyl-DL-$\alpha$-valine의 공중합체에 관하여-)

  • 최창남;홍성일
    • Textile Science and Engineering
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    • v.20 no.5
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    • pp.1-8
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    • 1983
  • Acrylonitrile (An) was copolymerized with N-methacryloyl-DL-$\alpha$-valine (MAV) in order to increase moisture regain and impart an affinity for acid dyes. MAV was prepared by Schotten-Baumann reaction of DL-$\alpha$-valine with methacryloyl chloride. The identification of MAV was conformed by IR spectrum, melting point and elemental analysis. Monomer reactivity ratios were calculated and inherent viscosity, tensile strength, moisture regain, dye absorption for acid dye, and decomposition temperatures of copolymers were investigated. The moisture regain, dye absorption for acid dye, inherent viscosity were investigated. The moisture regain, dye absorption for acid dye, inherent viscosity were increased, but tensile strength, decomposition temperature were decreased in proportion to the mole % of MAV.

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Identification of Essential Amino acid Residues in Valine Dehydrogenase from Streptomyces albus

  • Hyun Chang-Gu;Kim Sang-Suk;Suh Joo-Won
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.50-53
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    • 2006
  • Cys-29 and Cys-251 of Streptomyces albus valine dehydrogenase(ValDH) were highly conserved in the corresponding region of $NAD(P)^+$-dependent amino acid dehydroganase sequences. To ascertain the functional role of these cysteine residues in S. albus ValDH, site-directed mutagenesis was performed to change each of the two residues to serine. Kinetic analyses of the enzymes mutated at Cys-29 and Cys-251 revealed that these residues are involved in catalysis. We also constructed mutant ValDH by substituting valine for leucine at 305 by site-directed mutagenesis. This residue was chosen, because it has been proposed to be important for substrate discrimination by phenylalanine dehydrogenase (PheDH) and leucine dehydrogenase (LeuDH). Kinetic analysis of the V305L mutant enzyme revealed that it is involved in the substrate binding site. However it displayed less activity than the wild type enzyme toward all aliphatic and aromatic amino acids tested.

A study on Determination Method of (N-2-hydroxy-ethyl)valine(HEV) in Hemoglobin Adducts for Biological Monitoring of Ethylene Oxide Exposure

  • Lee, Jin-Heon;Shin, Ho-Shang;Ahn, Hye-Sil
    • Proceedings of the Korean Environmental Health Society Conference
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    • pp.337-340
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    • 2005
  • Ethylene oxide is a genotoxic carcinogen with widespread uses as industrial chemical intermediate and gaseous sterilant. 2-hydroxyethylated N-terminal valine in Hb is a good biomarker for biological monitoring of ethylene oxide exposure, because of its stability. We studied the determination method of (N-2-hydroxy-ethyl)valine in hemoglobin adduct by using GC/MS. PFPITC and TBMS were used as appropriate derivatives. Ethylene oxide formed Hb adducts as (N-2-hydroxy-ethyl)valine(HEV) in mouse with ethylene oxide inhalation exposure. Standard HEV can be synthesized with 2-amino-ethanol and 2-bromo-3-methylbutyric acid. GC/MS can measured them after derivatization with pentafluorophenylisothiocianate(PFPITC) and N-(tertiary butyl dimethylsiiyl)-N-methyl-trifluoroacetamide(TBDMS-TFA) by using Edman procedure. Concentrations of Hb adduct were proportionally increased with exposure levels. They were 230${\pm}$35(nmol g$^{-1}$ globin) and 410${\pm}$72(nmol $g^{-1}$ globin) at 200ppm and 400ppm ethylene oxide inhalation exposure, respectively.

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Studies on the Components Korean Sake (Part 2) -Detection of the Free Amino Acids in Takju by Paper Partition Chromatography- (한국(韓國) 주류성분(酒類成分)에 관(關)한 연구(硏究) (제2보(第2報)) -Paper Chromatography에 의(依)한 탁주중(濁酒中)의 유리(遊離) Amino 산(酸)의 검색(檢索)-)

  • Kim, Chan-Jo
    • Applied Biological Chemistry
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    • v.9
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    • pp.59-64
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    • 1968
  • Takju (Korean native Sake) was mashed with two different materials. One of the material was polished rice and Nuruk(mold wheat), the other one was corn and Nuruk. The amino acids in those fermenting mashes were identified by paper partition chromatography at regular intervals. The results were summarized as follows; a) Following 14 kinds of amino acids were identified in the mash of rice material; lysine, valine, proline, leucine, serine, glycine, aspartic acid, alanine, cystine, tyrosine, histidine, glutamic acid, tryptophan and phenylalanine b) Following 12 kinds of amino acids were identified in the mash of corn material; lysine, valine, proline, leucine, serine, glycine, aspartic acid, alanine, cystine, histidine, argrinine and tryptophan. c) The main amino acids in the Takju mash according to the color density of the each amino acid spot on the paper chromatograms were checked as lysine, valine, leucine, serine, proline and glycine.

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Modeling and Measurements of the Activity Coefficients and Solubilities of Amino Acids in the L-valine/electrolyte and L-proline/electrolyte Aqueous Solutions (L-Valine/전해질 및 L-Proline/전해질 수용액에서 아미노산의 활동도계수와 용해도의 측정 및 모델링)

  • Lee, Bong-Seop;Kim, Ki-Chang
    • Korean Chemical Engineering Research
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    • v.50 no.1
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    • pp.93-105
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    • 2012
  • Activity coefficients and solubilities of L-Valine and L-Proline in aqueous solutions containing each of four electrolytes such as NaCl, KCl, $NaNO_3$ and $KNO_3$ were measured at 298.15 K. The measurements of activity coefficients were carried out in the electrochemical cell coupled with two ion-selective electrodes (cation and anion), and the solubilities were measured by the gravimetric analysis of saturated solutions in equilibrium with the solid phase of amino acid. The measured activity coefficients of electrolytes and amino acids were correlated with the theoretical thermodynamic model presented in the previous work [Korean Chem. Eng. Res. 48(4), 519(2010)]. It was found that the activity coefficients of amino acids and electrolytes described based on the our previous model were well agreeable with experimental data. Also the experimental solubility data of L-Valine and L-Proline were successfully correlated with the thermodynamic relation mentioned in the previous work.