• Title, Summary, Keyword: Triiodothyronine($T_3$)

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A Study on the Specific Hormone Characteristics on the Blood in Four Types of Physical Constitution (체질(體質)에 따른 혈중(血中) Hormone 특성(特性)에 관한 연구(硏究))

  • Kim, Dae Seong;Kim, Gyeong-Yo;Han, Jong-Hyeon
    • Journal of Sasang Constitutional Medicine
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    • v.4 no.1
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    • pp.193-212
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    • 1992
  • Comparative hormones quantities on the blood analysis was carried out to investigate the hormones specific characters on the blood in four types of physical constitution. Thyroid-stimulating (TSH), triiodothyronine($T_3$), triiodothyronine uptake($T_3$ uptake), free triiodothyronine(free $T_3$), thyroxine($T_4$), free thyroxine (free $T_4$), thyroxine-binding globulin(TBG), adrenocorticotropic hormone(ACTH), luteinizing hormone(LH), testosterone were measured. The results obtained were summarized as follows: 1. The value of thyroid-stimulating hormone(TSH) on the constitution didn't shown significant difference, while the value of So-EUM-IN was increased and SO-YANG-IN was decreased. 2. The value of triiodothyronine($T_3$) on the constitution didn't shown significant difference, while the value of TAE-EUM-IN was decreased. 3. The value of triiodothyronine uptake($T_3$ uptake) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-EUM-IN, TAE-EUM-In and SO-YANG-IN showed significant difference. 4. The value of free triiodothyronine(free $T_3$) on the constitution didn't shown signifcant difference, but between the value of TAE-EUM-IN and SO-EUM-In, TAE-EUM-In and SO-YANG-IN showed significant difference. 5. The value of thyroxine($T_4$) on the constitution didn't shown significant difference, while the value of TAE-EUM-IN and SO-YANG-IN showed difference. 6. The value of free thyroxine(free $T_4$) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-YANG-IN showed significant difference. 7. The value of thyroxine-binding globulin(TBG) on the constitution didn't shown significant difference. 8. The value of adrenocorticotropic hormone(ACTH) on the constitution didn't shown significant difference. 9. The value of luteinizing hormone(LH) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-EUM-IN, TAE-EUM-IN and SO-YANG-IN showed significant difference. 10. The value of testosterone on the constitution didn't shown significant difference.

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Efficient Preparation of Radioiodine Labelled 3,5,3'-Triiodothyronine and Thyroxine for Medical Use

  • Kim, Jaerok;Kim, Tae-Ho
    • Nuclear Engineering and Technology
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    • v.7 no.2
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    • pp.127-133
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    • 1975
  • For isotopic exchange labelling of 3,5,3'-triiodothyronine (T$_3$) and thyroxine (T$_4$) with radioiodide in the presence of molecular iodine, T$_3$:I$_2$ or T$_4$:I$_2$ molar ratios, pH, and reaction time are. considered to be important factors. A modified labelling and separation method is proposed in present paper, by which T$_3$-$^{125}$ I and T$_4$-$^{125}$ I can be obtained with the mean labelling yields of 45%, and 50%, respectively. The whole reaction products can be separated by adoption of thin-layer chromatography technique using silica gel plate and the solvent system composd of chloroform, methanol and ammonia.

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A Prospective Study on the Changes of Serum 3,5,3'-Triiodothyronine Levels after Cardiopulmonary bypass in Pediatric Age (소아환자에서 개심술후 혈중 3,5,3'- 삼옥소타이로닌 [triiodothyronine] 농도 변화에 관한 연구)

  • Kim, Yeong-Tae;No, Jun-Ryang
    • The Korean Journal of Thoracic and Cardiovascular Surgery
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    • v.25 no.11
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    • pp.1209-1217
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    • 1992
  • Triiodothyronine[T3] is an important regulator of the tissue metabolism, and may have potential use as an inotropic agent. The change of serum T3 level was studied in the pediatric age patients after cardiopulmonary by pass. Thyroid function was tested pre-operatively in 33 patients and total triiodothyronine[TT3] levels were serially measured during and after cardiopulmonary bypass[CPB]. After correction of dilutional effect, effects of various factors on the TT3 levels were analyzed. Abrupt fall of TT3 level was demonstrated at 15 minutes after CPB[80.1$\pm$5.9ng/dL] from the initial level of 133.6$\pm$5.3ng/dL, with some recovery at 6 hours[115.4$\pm$6.7ng/dL]. After then, gradual decrease occured reaching to the level of 77.2$\pm$4.2ng /dL at 24 hours. These falls of the TT3 after CPB were statistically significant. [p<0.01 ANOVA] Statistically significant correlation were found between the degree of hemodilution and TT3 concentration at 15 and 30 minutes after CPB[p<0.05]. But, other factors were analyzed to have no effect on TT3 levels. As the degree of the hemodilution increases, TT3 decreased less. This observation probably supports the fact that decrease of TT3 during CPB may be a result of sequestration of T3 into peripheral tissue. Although it was not statistically significant[p=0.08], the fall of TT3 was greater in the group to which plasmanate was added, than those not added. This finding seemed to be due to the increase of albumin and other thyroid-hormone-binding-proteins in the serum. Increase of these binding proteins might potentiate the sequestration of T3 into the liver and the kidney from serum, and as a consequence, decrese the serum TT3 level further.

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Physiological Studies on Adaptation of Tilapia(Oreochromis miloticus) in the Various Salinities I. Endocrine Changes (틸라피아의 해수순치에 관한 생리학적 연구 I. 내분비학적 변화)

  • 윤종만;조갑민;박홍양
    • Korean Journal of Animal Reproduction
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    • v.16 no.4
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    • pp.353-361
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    • 1993
  • This study was taken to examine external changes, behavioral changes, and endocrine changes such as estradiol-17$\beta$, progesterone, T4 and T3 of female Oreochromis niloticus living in 0$\textperthousand$, 10$\textperthousand$, 20$\textperthousand$, and 30$\textperthousand$ salt concentrations, respectively. The results obtained in these experiments were summarized as follows. In seawater obtained in these experiments were summarized as follows. In seawater challenge test, any fish didn't die in each group such as 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$. When fish were adapted from 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, external body color of fish changed from dark-striped to light-grey color. At the same time, thyroxine and triiodothyronine concentrations significantly(P<0.05) increased, and then were at the highest level in 30 salinity. When fish were adapted from 0$\textperthousand$ to 10$\textperthousand$, feed intake of fish started from the fourth day. From 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, estradiol-17$\beta$ levels were increased gradually. When fish was adapted from 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, the levels of each progesterone didn't show significant change, and especially showed the lowest peak in 20$\textperthousand$. The greatest thyroxine activity(T4) was observed in 30$\textperthousand$. The levels of and triiodothyronine(T3) significantly changed in all salinities, and its level was at the highest peak in 30$\textperthousand$ salinity. Correlation coefficients between serum progesterone and triiodothyronine in 10$\textperthousand$ and 30$\textperthousand$ were +0.677 and +0.843, respectively. Correlation coefficient of serum thyroxine(T4) and triiodothyronine(T3) individuals in 10$\textperthousand$ was +0.768, and +0.843, respectively.

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Comparative study of acute in vitro and short-term in vivo triiodothyronine treatments on the contractile activity of isolated rat thoracic aortas

  • Lopez, Ruth Mery;Lopez, Jorge Skiold;Lozano, Jair;Flores, Hector;Carranza, Rosa Angelica;Franco, Antonio;Castillo, Enrique Fernando
    • The Korean Journal of Physiology and Pharmacology
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    • v.24 no.4
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    • pp.339-348
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    • 2020
  • We aimed to characterize the participation of rapid non-genomic and delayed non-genomic/genomic or genomic mechanisms in vasoactive effects to triiodothyronine (T3), emphasizing functional analysis of the involvement of these mechanisms in the genesis of nitric oxide (NO) of endothelial or muscular origin. Influences of in vitro and in vivo T3 treatments on contractile and relaxant responsiveness of isolated rat aortas were studied. In vivo T3-treatment was 500 ㎍·kg-1·d-1, subcutaneous injection, for 1 (T31d) and 3 (T33d) days. In experiments with endothelium-intact aortic rings contracted with phenylephrine, increasing concentrations of T3 did not alter contractility. Likewise, in vitro T3 did not modify relaxant responses induced by acetylcholine or sodium nitroprusside (SNP) nor contractile responses elicited by phenylephrine or angiotensin II in endothelium-intact aortas. Concentration-response curves (CRCs) to acetylcholine and SNP in endothelium-intact aortic rings from T31d and T33d rats were unmodified. T33d, but not T31d, treatment diminished CRCs to phenylephrine in endothelium-intact aortic rings. CRCs to phenylephrine remained significantly depressed in both endothelium-denuded and endothelium-intact, nitric oxide synthase inhibitor-treated, aortas of T33d rats. In endothelium-denuded aortas of T33d rats, CRCs to angiotensin II, and high K+ contractures, were decreased. Thus, in vitro T3 neither modified phenylephrine-induced active tonus nor CRCs to relaxant and contractile agonists in endothelium-intact aortas, discarding rapid non-genomic actions of this hormone in smooth muscle and endothelial cells. Otherwise, T33d-treatment inhibited aortic smooth muscle capacity to contract, but not to relax, in an endothelium- and NO-independent manner. This effect may be mediated by delayed non-genomic/genomic or genomic mechanisms.

Development of Homogeneous Enzyme Immunoassay for Serum 3,5,3'-Triiodothyronine Determination (혈청 3,5,3'-Triiodothyronine 측정을 위한 효소-면역 분석의 개발 연구)

  • 이희주
    • YAKHAK HOEJI
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    • v.27 no.2
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    • pp.117-124
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    • 1983
  • For development of $EMIT-T_{3}$ assay, the conjugation of 3, 5, 3'-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of $T_{3}$-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, $0^{\circ}C$ during overnight. The obtained $T_{3}$-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40-70% with various $anti-T_{3}$ antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for $T_{3}$ assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.

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RELATIONSHIP BETWEEN SOME CIRCULATING HORMONES, METABOLITES AND MILK YIELD IN LACTATING CROSSBRED COWS AND BUFFALOES

  • Jindal, S.K.;Ludri, R.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.2
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    • pp.239-248
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    • 1994
  • To study the relationship between certain hormones and metabolites and between hormones and milk yield during different stage of lactation, six lactating Karan Swiss cows and six Murrah buffaloes were maintained. Growth hormone, insulin, $T_3$, $T_4$, glucose, BHBA, NEFA and milk yield were studied. Highly negative relationship of growth hormone with insulin and triiodothyronine in cows and marginally negative in buffaloes suggest that insulin and triiodothyronine aid in the process of partitioning of nutrients towards milk production through reducing the demands of nutrients by peripheral tissue. The significant and negative correlation of growth hormone with dry matter intake in both the species suggest that the availability of nutrients from the digestive tract play a role in the regulation of growth hormone secretion. Positive relationship of growth hormone with non esterified fatty acids in both the species suggest that high growth hormone levels may result in fat mobilization and thereby increase the availability of energy precursors for milk synthesis. Insulin was negatively correlated with milk yield and lactose content and positively with milk fat and protein but the degree of relationship varied. In both the species the relationship between triiodothyronine and milk yield was negative and between thyroxine and milk yield was positive. However, it was significant only in cows and not in buffaloes. Thyroxine was positively correlated with beta-hydroxybutyrate and non-esterified fatty acids with milk yield in both the species.

Maternal Serum Concentrations of Total Triiodothyronine, Tetraiodothyronine and Cortisol in Different Status of Pregnancy During Late Pregnancy in Ettawah-Cross Does

  • Manalu, W.;Sumaryadi, M.Y.;Kusumorini, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.4
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    • pp.385-390
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    • 1997
  • Fifteen Ettawah-cross does were used to study maternal serum concentrations of total triiodothyronine ($T_3$), teraiodothyronine ($T_4$) and cortisol in different status of pregnancy (nonpregnant, aborted, single and twin-bearing does) during late pregnancy. Analysis of the data indicated that here was no significant changes in total $T_3$, $T_4$, and cortisol concentrations with the advance of pregnancy. Concentrations of $T_3$, $T_4$, and cortisol decreased by 38.9, 34.9, and 32.6%, and 12.0, 15.7 and 27.6%, and 41.6, 44.0, and 43.7% in the aborted, single and, twin-bearing, respectively, as compared to those nonpregnant does. These was no significant difference in concentrations of $T_3$ and cortisol between aborted, single and twin-bearing does, and in those of $T_4$ between aborted and single-bearing does. However, $T_4$ concentrations in twin-bearing were lower by 17.7 and 14.1% than those in aborted and single-bearing does, respectively. The decreased concentrations of thyroid hormones in pregnant does suggested that fetus could have increased iodine uptake from maternal circulation causing a decrease in the availability of this nutrient for synthesis of maternal thyroid hormones. The decreased concentrations of cortisol could have been associated with the increased metabolism of the hormone to regulate nutrients influx into the placenta of pregnant does.

Growth and Activities of Larvae Born from the Triiodothyronine-Injected Parturient Rockfish, Sebastes schlegeli (출산기 모체에게 triiodothyronine (T3) 주사하여 얻은 조피볼락, Sebastes schlegeli 자어의 성장 및 활성)

  • Kang Duk Young;Chang Young Jin;Hur Jun Wook;Min Byung Hwa
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.551-556
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    • 2002
  • We have experimentally evaluated the content of thyroid hormones (THs), the growth and activity of larval rockfish (Sebastes schiegeli) born from parturient mother fish injected with 20 $\mu$g/g BW of 3,5,3-triiodo-L-thyronine ($T_{3}$). There was no difference of L-thyroxine ($T_{4}$) levels between controls (sham control and control) and $T_{3}$ groups in yolk-sac larvae just born from broodstock, while $T_{3}$level of $T_{3}$ group was significantly increased compared with controls. In addition, the both larval $T_{3}$ and $T_{4}$ levels in $T_{3}$ group were always higher than those of control during experimental period. Also, there were significant differences in the development of larvae from the two groups. The larval growth in total length and body weight according to the elapsed days after parturition showed the linear and curve equations, respectively, and the slopes of $T_{3}$ group were significantly higher than those of control. The survival rate of larvae in $T_{3}$ group was higher than that of control. Although the survival rate in $T_{3}$ group under the condition of starvation was significantly depressed compared with the control, larval swimming index in $T_{3}$ group was higher than control. Finally, these findings suggest that the exogenous $T_{3}$ could be transferred into hatched larvae in parturient rockfish by maternal injection, and subsequently the exogenous hormone could play some roles on physiological metabolism of larvae after parturition and may confer a distinct advantage to the fragile larvae during the early developmental stage.