• Title, Summary, Keyword: Tricholoma matsutake

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A Study on the Pine Forest Conditions Growing Tricholoma matsutake in Korea (한국(韓國)의 송이 발생(發生) 송림(松林)의 현황(現況)에 관한 조사(調査) 연구(硏究))

  • Lee, Tai-Soo;Kim, Young-Ryoun;Jo, Jae-Myeong;Lee, Ji-Yul;Ogawa, M.
    • The Korean Journal of Mycology
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    • v.11 no.1
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    • pp.39-49
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    • 1983
  • As Dr. Ogawa visited to Korea for joint research on Tricholoma matsutake, authors travelled 15 main producing districts of Tricholoma matsutake and had field survey on sample areas in these districts from September 23 to October 3 1981. The object of this investigation were to understand the actual conditions of pine forest and growing Tricholoma matsutake in Korea so as to find out relevant treatment of pine forest where the Tricholoma matsutake were growing. As a results, present environmental conditions such as weather, topography, soils, pine forest, vegetations, pine mushrooms(Tricholoma matsutake), other fungi and the relationship between Tricholoma matsutake and the diseases of pine forest were investigated. Model figures of topography and forest type on surveyed area have been drawn up. Some countermeasures to improve pine forest conditions in the aspect of producing Tricholoma matsutake were suggested through the prediction on the transition of pine forest.

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Study on Activities of Antioxidant and Anticancer of Germanium-fortified Tricholoma matsutake Myceliumm (게르마늄 강화 송이균사체의 항산화 및 항암 활성에 관한 연구)

  • Kim, Hae-Ja;Kim, Wan-Gyeom;Cho, Hwa-Eun;Choi, Yun-Hee;Lee, Ki-Nam;Chong, Myong-Soo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.2
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    • pp.473-479
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    • 2009
  • The purpose of this study was evaluated physiological activity effect of organic germanium in culture broth of germanium-fortified Tricholoma matsutake mycelium and germanium-fortified yeast. Proliferation Tricholoma matsutake mycelium and yeast was inhibited by addition of germanium. Contents of organic germanium in Tricholoma matsutake mycelium and yeast was increased in dose-dependent manner. And low concentration(1,000 ppm) of germanium in mycelium was almost changed organic germanium. In the result of antioxidant activity as SOD-like activity, contents of total polyphenol compound and electron donating ability, activity of germanium-fortified Tricholoma matsutake mycelium was higher than that of germanium-fortified yeast. To evaluate of antitumor effects in vitro, we examined nitric oxide production of Raw 264,7 cell and cytotoxicity of HT1080 cell by MTT assay. Nitric oxide production of germanium-fortified Tricholoma matsutake mycelium was shown low level in low concentration(1,000 ppm) than other groups. The anticancer effect of germanium-fortified Tricholoma matsutake mycelium on HT 1080 cell was indicated a strong inhibitory effect in low concentration(1,000 ppm). These results suggest that organic germanium in culture broth of germanium-fortified Tricholoma matsutake mycelium has valuable physiological activities as antioxidant and anticancer effect, and it was higher than that of germanium-fortified yeast.

Effects of Germanium-fortified Tricholoma matsutake Mycelium and Yeast on Blood Glucose and Serum Lipid in Streptozotocin-Induced Diabetic Rats (게르마늄 강화 송이균사체와 효모가 Streptozotocin 유발 당뇨 쥐의 혈당 및 혈청 지질에 미치는 영향)

  • Kim, Wan-Gyeom;Kim, Hae-Ja;Chong, Myong-Soo;Cho, Hwa-Eun;Choi, Yun-Hee;Lee, Ki-Nam
    • Journal of Society of Preventive Korean Medicine
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    • v.13 no.2
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    • pp.89-102
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    • 2009
  • Objectives : The purpose of this study was evaluated hypoglycemic effect of culture broth of germanium-fortified Tricholoma matsutake mycelium and yeast. Methods : We examined $\alpha$-glucosidase inhibitory activity, blood glucose level, concentration of serum lipid, and serum metabolic variables of Tricholoma matsutake mycelium and yeast fortified Germanium. in streptozotocin induced diabetic rat. Results : In the $\alpha$-glucosidase inhibitory activity, germanium-fortified yeast was significantly higher than germanium-fortified Tricholoma matsutake mycelium. The hypoglycemic effects of germaniumfortified Tricholoma matsutake mycelium was higher than germanium-fortified yeast. The activity of alkaline phosphatase(ALP), aspartate aminotransferase(AST) and alanine aminotransferase(ALT) was significantly lower in the germanium-fortified Tricholoma matsutake mycelium and yeast than in diabetic control(DC) group and diabetic positive control(PC) group. The concentration of total cholesterol and triglyceride of germanium-fortified yeast was significantly lower than germanium-fortified Tricholoma matsutake mycelium, DC group and PC group. Conclusions : The results suggest that germanium-fortified Tricholoma matsutake mycelium and yeast have improvement effects in blood glucose, serum lipid and liver function.

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Biotope Mapping of Pinus densiflora Based on Growth Environment of Tricholoma matsutake - A Case Study of Yangyang-gun, Kang Won-do - (송이 생육환경 특성을 고려한 소나무비오톱지도 작성 연구 - 강원도 양양군을 사례로 -)

  • Han, Bong-Ho;Park, Seok-Cheol;Kwak, Jeong-In;Kim, Bo-Hyun;Lee, Kyong-Jae
    • Korean Journal of Environment and Ecology
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    • v.25 no.2
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    • pp.211-226
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    • 2011
  • The purpose of this paper was to ensure the basis for effective management of Tricholoma matsutake mountain province, to perform biotope mapping of Pinus densiflora based on growth environment of Tricholoma matsutake, target a cluster of Yangyang-gun, Kang Won-do. Study Methods were to review on growth and environmental characteristics of Tricholoma matsutake through internal and external documents and to identify vegetational structure and soil characteristics. This paper studied growth structure and soil environment of Pinus densiflora forest where a farm of production area for Tricholoma matsutake of in order to set the standard of Pinus densiflora biotope. Mapping standards were derived by separating of landform conditions, soil conditions, vegetation conditions. Biotope types were divided into possible production area for Tricholoma matsutake and potential production area for Tricholoma matsutake, possible production area for Tricholoma matsutake were Pinus densiflora biotope in landform and soil structure that enables Tricholoma matsutake production and Single-layered Pinus densiflora biotope of less than 30cm(DBH)-Tree species that other shrub is dominant in shrub layer, Multi-layered Pinus densiflora biotope that Pinus densiflora forest was predominant in understrory layer. Potential production area for Tricholoma matsutake were single-layered Pinus densiflora biotope of more than 30cm(DBH) in landform that enables Tricholoma matsutake production, Pinus densiflora biotope with Quercus predominant in the understrory layer, single-layered Pinus densiflora biotope with Quercus predominant in shrub layer, inappropriate vegetation structure area that the induction of production of Tricholoma matsutake was possible through future vegetation management. According to the research results, Pinus densiflora forest were divided into 16 types; 6 types of possible Tricholoma matsutake production areas, 9 potential Tricholoma matsutake production areas and 16 types of areas where Tricholoma matsutake production was impossible. Possible production areas account for 15.48%, or $9.8km^2$ out of the total Pinus densiflora forest while potential production areas take up 32.42%, or $20.52km^2$, and areas where Tricholoma matsutake production was impossible was 52.10%, or $32.97km^2$.

Effect of Complex Nitrogen Source on Mycelial Growth of Tricholoma matsutake DGUM 26001 (송이(Tricholoma matsutake DGUM 26001) 균사의 생육에 미치는 복합 질소원의 영향)

  • Min, Eung-Gi;Chung, Kwang-Kyo;Han, Yeong-Hwan
    • The Korean Journal of Mycology
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    • v.26 no.3
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    • pp.361-364
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    • 1998
  • Among the organic sources of nitrogen tested, yeast extract and soytone were excellent for the mycelial growth of Tricholoma matsutake DGUM 26001. The mycelial growth was enhanced, when yeast extract at the concentration up to 1.0% was added to the starchpyridoxine medium. After 30-day cultivation of the mycelia at $24^{\circ}C$ in the medium supplemented with yeast extract, 518 mg/50 ml of dry mycelia could be harvested.

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Inhibitory Effects of Cultured Tricholoma matsutake Mycelia on Melanin Biosynthesis (송이버섯 배양 균사체의 멜라닌 생성억제효과)

  • Choi, Sang-Yoon;Kim, Na-Na;Kim, Young-Eon;Lee, Yeon-Mi;Kim, Soon-Jung;Kim, Jae-Ho
    • Korean Journal of Food Science and Technology
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    • v.43 no.2
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    • pp.240-242
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    • 2011
  • In this study, liquid culture of Tricholoma matsutake mycelia was performed via biomass production, and its inhibitory effects on melanin biosynthesis were evaluated. The Tricholoma matsutake mycelia extract inhibited 38.6% of tyrosinase activity at 100 ppm, which is higher than that of extracelluar medium at same dose. In addition, when 100 ppm of Tricholoma matsutake mycelia extract was treated to melan-a cells for 3 days, 19% of melanin production was reduced without cell toxicity. These results suggested that cultured Tricholoma matsutake mycelia might be useful as a skin depigmenting material.

Development of Specific Primer for Tricholoma matsutake

  • Kim, Jang-Han;Han, Yeong-Hwan
    • Mycobiology
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    • v.37 no.4
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    • pp.317-319
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    • 2009
  • In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'- GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.

Biotope Types and Mapping for Ecological Management of Tricholoma matsutake Production Area: The Case of Expressway in Yangyang-gun, Gangwon-do (송이생산지의 생태적 관리를 위한 소나무비오톱 유형화 및 지도 작성: 강원도 양양군 동서고속도로 건설구간을 중심으로)

  • Kim, Jeong-Ho;Choi, Song-Hyeon;Yoon, Yong-Han
    • Journal of Environmental Policy
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    • v.11 no.3
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    • pp.25-47
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    • 2012
  • This research is to suggest an ecological management methods by classification of biotope type and its mapping for Tricholoma matsutake. The target area was construction site of the East Hongchun - Yangyang Highway (Yangyang section) where massive production of Tricholoma matsutakes is in place. Specifically, the 700 m long range of area around the road route, Yangyang section, which is over a total area of $19,79km^2$. The flow of this research was as follows: first studied were the characteristics of the Yangyang Tricholoma matsutakes; second, reviews on previous studies on Tricholoma matsutakes; third, the formulation of standards and processes of biotope type considering Tricholoma matsutake production; and fourth, mapping of biotope considering the production area of Tricholoma matsutake. In 24.77% of the region, slope angle measured $30{\sim}40^{\circ}C$, which is suitable for Tricholoma matsutake production. Also, 17.44% of the region had southward vegetation structures, and as for the Pinus densiflora; 26.00% had average receipts; the tree ages was $38{\pm}8.34$ years; and the average density of canopy layer was $9.55{\pm}4.89/100m^2$. The soil acidity (pH) values of 5.0 to 5.6(47.96%) > 5.6 to 7.0(42.90%) > 4.0 to 5.0(9.14%) were slightly high for Tricholoma matsutake growth; but 57.10% of the area had a soil pH of 4.0-5.6. The organic horizon (A0 layer) was $3.39{\pm}2.14cm$, and the mean depths were 4-6cm(78.03%) > 0-2cm(18.10%) > 2-4cm(3.87%). Based on the results of previous studies and field researches on the presence of vegetation (the pine dominance ratio), the topographic structure (the terrain characteristics and slopes), the receipt, the soil structure (the soil acidity and the organic layers interposed between the depths), and the vegetation density, six clusters based on the identified types of biotopes are produced in this paper. Area of distribution according to the type of biotope was Tricholoma matsutake of the potential-production (II) biotope (32.86%) > Tricholoma matsutake of the inadequate-production biotope (22.17%) > Tricholoma matsutake of the adequate (II)-production biotope (17.79%) > Tricholoma matsutake of the adequate (I)-production biotope (14.86%) > Tricholoma matsutake of the potential-production (I) biotope (9.77%) > Tricholoma matsutake of the produced biotope (2.55%).

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Effect of Carbon sources and Vitamins on Mycelial Growth of Tricholoma matsutake DGUM 26001 (송이균사(Tricholoma matsutake DGUM 26001)의 생육에 미치는 탄소원 및 비타민의 영향)

  • Lee, Chang-Yun;Hong, Oun-Pyo;Jung, Myung-Jun;Han, Yeong-Hwan
    • The Korean Journal of Mycology
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    • v.25 no.3
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    • pp.226-232
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    • 1997
  • The mycelium was isolated from the fruiting body of Tricholoma matsutake collected from Mt. Namsan, Kyongju and it was named as Tricholoma matsutake DGUM 26001. For the mycelial growth of T. matsutake DGUM 26001, the complex media, yeast-malt extract medium and Czapek-Dox medium supplemented with yeast extract, were excellent. The media such as nutrient glucose medium, mushroom complex medium, and Tricholoma matsutake medium (TMM), were effective. However, There was no a mycelial growth in the media used for bacterial cultivation such as colombia medium, brain heart infusion medium, Luria-Bertani medium supplemented with glucose, and brucella medium. When carbohydrate as a carbon and energy source was supplemented in the TMM medium for the mycelial growth, starch as a polysaccharide was best. As a disaccharide, trehalose and maltose were excellent. Sorbitol, xylitol and glucose were excellent carbon sources of monosaccharose. When the mycelia were cultivated for 30 days at $24^{\circ}C$ in the TMM supplemented with 2.0% starch, the dry weight of the mycelia harvested was 8.85 g/L. When organic acid was given as a carbon source, only succinic acid was utilized. As a vitamin source, coconut water and pyridoxine were excellent. After 30 day-cultivation in the TMM medium, the dry weights with coconut water and pyridoxine were 8.65 and 8.32 g/L, respectively.

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Mycelial Growth Properties of Domestically Collected Ectomycorrhizal Tricholoma Mushrooms in Various Culture Conditions (다양한 배양 환경에 따른 국내 수집 외생균근성 Tricholoma속 종의 균사생장 특성)

  • Kang, Jung-A;Ka, Kang-Hyeon;Kim, Jun Young;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.46 no.3
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    • pp.271-280
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    • 2018
  • The ectomycorrhizal basidiomycete Tricholoma is one of mushroom groups that cannot be cultivated artificially. To use this mushroom as applicable resource for food production, it is necessary to obtain information about their mycelial growth properties in various environmental conditions. This study investigated the mycelial growth of four domestic isolates of Tricholoma species (T. bakamatsutake, T. fulvocastaneum, T. matsutake, T. terreum) at different physical and chemical conditions. The optimal physical conditions for their mycelia growth were found to be a temperature range of $20{\sim}25^{\circ}C$ and a pH range of 4.0~7.0 in dark condition. The growth of T. matsutake was retarded at high temperature ($30^{\circ}C$). Tests to determine the chemical factors that affected mycelial growth showed that the four Tricholoma spp. grew 1% saline. T. matsutake grew in up to 2% saline. In the presence of various heavy metals (50 ppm) and pesticides (suppliers' recommended concentration), mycelial growth was inhibited the most by cadmium and emamectin benzoate, respectively. However, all the four Tricholoma spp. grew with $Cu^+$. The growth of T. matsutake was not inhibited by abamectin, acetamiprid, and thiacloprid. Extracellular enzyme activities of amylase and ${\beta}$-glucosidase were detected only in T. bakamatsutake and T. fulvocastaneum. The results of the present study allowed us to determine suitable or harmful environmental conditions for the mycelial cultivation of the Tricholoma spp.