• Title, Summary, Keyword: Tissue Accumulation

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Kinetic of Copper Accumulation and Elimination in Rockfish (Sebastes schlegeli) Tissues Exposed to Dietary Copper

  • Kim Seong-Gil;Jee Jung-Hoon;Kim Sang-Gyu;Kang Ju-Chan
    • Fisheries and aquatic sciences
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    • v.6 no.4
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    • pp.213-219
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    • 2003
  • Experiments were carried out to investigate the accumulation and elimination changes in the tissue of juvenile rockfish (Sebastes schlegeli) after sub-chronic dietary Cu (0, 50, 125, 250 and 500 mg/kg) exposure for 60 days and depuration for 30 days. The profile of Cu accumulation in the tissue of rockfish was dependent on the exposure periods and Cu concentration. Liver of rockfish is a more important storage tissue than other tissues, and the order of Cu accumulation in tissues was liver > intestine > kidney > gill > muscle. The accumulation factors were increased with the exposure period in gill, intestine, liver, kidney and muscle. An inverse relationship was observed between the accumulation factor and the exposure concentrations in the gill, kidney and muscle. Cu elimination in tissues of rockfish were decreased with periods for the 30 days of depuration except kidney and muscle. The order of Cu elimination in organs during depuration was intestine > liver > gill.

On the Accumulation of Minerals with the Plant Species in a Reclaimed Land (干拓地 植物의 無機營養素 蓄積에 對하여)

  • Min, Byeong Mee
    • The Korean Journal of Ecology
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    • v.13 no.1
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    • pp.9-18
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    • 1990
  • For reaserching the factors of plants micro-distribution, accumulation of 5 minerals-total-nitrogen, phosphorus, potassium, sodium and calcium-for 19 plant species was investigated in reclaimed land, in western coast of Korea, In the five minerals, sodium contents were quite different among the species. Plant species were divided into 4 groups based on the sodium accumulation and accumulation site in plant tissue: Na accumultion type above-ground part>below-ground part : Triglochin maritimum, Chenopodium virgatum, Atriplex subcordata, Salicornia herbacea, Suaeda japonica, suaeda asparagoides, Limonium tetragonum, Aster tripolium, Artemisia scoparia, Sonchus brachyotus above-ground partbelow-ground part : Zoysia sinica, amagrostis epigeiosa bove-ground part

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Fluvastatin inhibits advanced glycation end products-induced proliferation, migration, and extracellular matrix accumulation in vascular smooth muscle cells by targeting connective tissue growth factor

  • Hwang, Ae-Rang;Nam, Ju-Ock;Kang, Young Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.22 no.2
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    • pp.193-201
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    • 2018
  • Connective tissue growth factor (CTGF) is a novel fibrotic mediator, which is considered to mediate fibrosis through extracellular matrix (ECM) synthesis in diabetic cardiovascular complications. Statins have significant immunomodulatory effects and reduce vascular injury. We therefore examined whether fluvastatin has anti-fibrotic effects in vascular smooth muscle cells (VSMCs) and elucidated its putative transduction signals. We show that advanced glycation end products (AGEs) stimulated CTGF mRNA and protein expression in a time-dependent manner. AGE-induced CTGF expression was mediated via ERK1/2, JNK, and Egr-1 pathways, but not p38; consequently, cell proliferation and migration and ECM accumulation were regulated by CTGF signaling pathway. AGE-stimulated VSMC proliferation, migration, and ECM accumulation were blocked by fluvastatin. However, the inhibitory effect of fluvastatin was restored by administration of CTGF recombinant protein. AGE-induced VSMC proliferation was dependent on cell cycle arrest, thereby increasing G1/G0 phase. Fluvastatin repressed cell cycle regulatory genes cyclin D1 and Cdk4 and augmented cyclin-dependent kinase inhibitors p27 and p21 in AGE-induced VSMCs. Taken together, fluvastatin suppressed AGE-induced VSMC proliferation, migration, and ECM accumulation by targeting CTGF signaling mechanism. These findings might be evidence for CTGF as a potential therapeutic target in diabetic vasculature complication.

Time Course Changes in Hepatic Fat Accumulation in High Fat Diet-fed C57BL/6 Mice: Comparison Analysis to the Markers of Systemic Insulin Resistance (고지방식이 섭취 마우스에서 간 중성지방 축적의 시간에 따른 변화: 인슐린저항성 지표들과의 상관관계 분석)

  • Bae, Eun Ju
    • YAKHAK HOEJI
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    • v.56 no.6
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    • pp.364-365
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    • 2012
  • Liver is the major organ to regulate the systemic glucose homeostasis and insulin resistance. Excess energy intake leads to triglyceride accumulation in adipose tissue first and subsequent accumulation in liver, resulting in obesity and type 2 diabetes. The representative pathological animal model for obesity associated insulin resistance is a high fat diet (HFD) fed mice model. Given the essential role of liver fat accumulation in developing systemic insulin resistance in obesity, I measured the liver triglyceride contents in HFD fed mice as a function of time. As such, in this report, I show the cause and effect relationship with regard to time during a HFD feeding between a variety of factors that are related to systemic insulin resistance including glucose intolerance, plasma insulin level and inflammatory gene expression in liver and adipose tissue.

Effects of Dietary Copper Exposure on Accumulation and Histopathological Change in Liver of Juvenile Rockfish, Sebastes schlegeli

  • Kim Jae-Won;Kim Seong-Gil;Kim Sang-Gyu;Song Seoung-Yeup;Kang Ju-Chan
    • Fisheries and aquatic sciences
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    • v.6 no.2
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    • pp.81-87
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    • 2003
  • Experiments were carried out to investigate the accumulation and the histopathological changes in liver of juvenile rockfish, S. schlegeli, after sub-chronic dietary Cu (0, 50, 125, 250 and 500mg/kg) exposure for 60 days. Cu accumulation in liver was significantly increased with dietary exposure period and concentration for 60 days, and has a linear relation with dietary exposure days. After 60 days of Cu dietary exposure, the Cu concentration in the liver was $75.9\pm12.05,\;126.29\pm22.11\;and\;360.44\pm45.26\;{\mu}g/g$ dry weight and was approximately 11-fold, 18-fold and 51-fold higher than in the control diet group at 125, 250 and 500 mg/kg Cu diet group. The accumulation factors were increased with the dietary exposure period in liver of rockfish. In the primary exposed stage, the effect of hepatic tissue in the rockfish exposed to dietary Cu observed enlargement of hepatocytes nuclei, activity of hepatic cells and the swelling of hepatic cells. While exposed time and concentration were increased, the distinct granulation, irregular shape and necrosis of hepatic cells were observed. It was observed that granule degeneration and necrosis showed a part of cells in hepatic tissue after 60 days at 500 mg/kg.

Effect of Cnidi rhizoma Boiling Extract Solution on Enzyme and Hormone of Plasma, and Liver in the Fatted Rats Induced by High Fat Dietary (천궁의 열수추출액이 고지방식이에 의한 흰쥐의 혈장 중 효소활성과 호르몬 및 간장의 지방축적에 미치는 영향)

  • 성태수;손규목
    • The Korean Journal of Food And Nutrition
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    • v.7 no.2
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    • pp.108-113
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    • 1994
  • This study was carried out to evaluate the effect of Cnidi rhizoma (CR) water extract on fat accumulation In fatted rats induced by the oral high fat administration for six weeks. To accomplish this evaluation, the serum and liver tissue have been examined for enzyme activity, cortisol and insulin level. The change of liver or tissue have been observed by the light microscope. GOT GPT and LDH activities were lower than the control group. Insulin and cortisol were higher than the control group, due to the fat accumulation. The liver of the control group observed by the tight microscope appeared to the fatty liver, but CR group showed some improvement of the fatty liver Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Cnidi rhizoma water extract.

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Real-time Voltammetric Assay of Cadmium Ions in Plant Tissue and Fish Brain Core

  • Ly, Suw-Young
    • Bulletin of the Korean Chemical Society
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    • v.27 no.10
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    • pp.1613-1617
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    • 2006
  • Optimum analytical conditions for cyclic voltammetry (CV) and square wave (SW) stripping voltammetry were determined using mercury-mixed carbon nanotube paste electrode (PE). The results approached the microgram working ranges of SW: 10.0-80.0 $ugL^{-1}$ and CV: 100-700 $ugL^{-1}$ Cd (II); working conditions of 300-Hz frequency, 100 mV amplitude, 1.6 V accumulation potential, 400 sec accumulation time, and 40 mV increment potential. First, analysis was performed through direct assay of cadmium ions deep into the fishs brain core and plant tissue in real time with a preconcentration time of 400 sec. The relative standard deviation of 10.0 $mgL^{-1}$ Cd (II) observed was 0.064 (n = 12) at optimum conditions. The low detection limit (S/N) was set at 0.6 $ugL^{-1}$ ($5.33{\times}10^{-9}$ M). The methods can be used in direct analysis in vivo or in real-time monitoring of plant tissue.

Effects of Testosterone on White Adipose and Liver Tissues in Male Castrated C57BL/6J Mice

  • Jeong, Sun-Hyo;Yoon, Mi-Chung
    • Biomedical Science Letters
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    • v.13 no.2
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    • pp.91-97
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    • 2007
  • Obesity is defined as increased mass of adipose tissue, conferring a higher risk of cardiovascular and metabolic disorders such as diabetes, hyperlipidemia, and coronary heart disease. To get a better understanding of the role of a male sex hormone testosterone on obesity, we thus measured the effects of testosterone on white adipose tissue (WAT) mass, adipocyte histology and hepatic lipid accumulation in male castrated (CAST) C57BL/6J mice. Compared to male CAST control mice, testosterone-treated mice had the decreased WAT mass and the increased the number of adipocytes. Especially, histological data showed that the adipocyte size was reduced in a dose-dependent manner and was most effective at dose 150 $\mu$g per mouse for testosterone. In addition, the administration of testosterone resulted in the inhibition of hepatic lipid accumulation compared with control mice. Our results suggest that testosterone regulates adipocytes development and hepatic lipid metabolism, resulting in the prevention of obesity in male CAST mice.

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Pear pomace ethanol extract improves insulin resistance through enhancement of insulin signaling pathway without lipid accumulation

  • You, Mi-Kyoung;Kim, Hwa-Jin;Rhyu, Jin;Kim, Hyeon-A
    • Nutrition Research and Practice
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    • v.11 no.3
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    • pp.198-205
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    • 2017
  • BACKGROUND/OBJECTIVES: The anti-diabetic activity of pear through inhibition of ${\alpha}-glucosidase$ has been demonstrated. However, little has been reported about the effect of pear on insulin signaling pathway in obesity. The aims of this study are to establish pear pomace 50% ethanol extract (PPE)-induced improvement of insulin sensitivity and characterize its action mechanism in 3T3-L1 cells and high-fat diet (HFD)-fed C57BL/6 mice. MATERIALS/METHODS: Lipid accumulation, monocyte chemoattractant protein-1 (MCP-1) secretion and glucose uptake were measure in 3T3-L1 cells. Mice were fed HFD (60% kcal from fat) and orally ingested PPE once daily for 8 weeks and body weight, homeostasis model assessment of insulin resistance (HOMA-IR), and serum lipids were measured. The expression of proteins involved in insulin signaling pathway was evaluated by western blot assay in 3T3-L1 cells and adipose tissue of mice. RESULTS: In 3T3-L1 cells, without affecting cell viability and lipid accumulation, PPE inhibited MCP-1 secretion, improved glucose uptake, and increased protein expression of phosphorylated insulin receptor substrate 1 [p-IRS-1, ($Tyr^{632})$)], p-Akt, and glucose transporter type 4 (GLUT4). Additionally, in HFD-fed mice, PPE reduced body weight, HOMA-IR, and serum lipids including triglyceride and LDL-cholesterol. Furthermore, in adipose tissue, PPE up-regulated GLUT4 expression and expression ratio of p-IRS-1 ($Tyr^{632})/IRS$, whereas, down-regulated p-IRS-1 ($Ser^{307})/IRS$. CONCLUSIONS: Our results collectively show that PPE improves glucose uptake in 3T3-L1 cells and insulin sensitivity in mice fed a HFD through stimulation of the insulin signaling pathway. Furthermore, PPE-induced improvement of insulin sensitivity was not accompanied with lipid accumulation.

Rosehip Extract Inhibits Lipid Accumulation in White Adipose Tissue by Suppressing the Expression of Peroxisome Proliferator-activated Receptor Gamma

  • Nagatomo, Akifumi;Nishida, Norihisa;Matsuura, Yoichi;Shibata, Nobuhito
    • Preventive Nutrition and Food Science
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    • v.18 no.2
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    • pp.85-91
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    • 2013
  • Recent studies have shown that Rosa canina L. and tiliroside, the principal constituent of its seeds, exhibit anti-obesity and anti-diabetic activities via enhancement of fatty acid oxidation in the liver and skeletal muscle. However, the effects of rosehip, the fruit of this plant, extract (RHE), or tiliroside on lipid accumulation in adipocytes have not been analyzed. We investigated the effects of RHE and tiliroside on lipid accumulation and protein expression of key transcription factors in both in vitro and in vivo models. RHE and tiliroside inhibited lipid accumulation in a dose-dependent manner in 3T3-L1 cells. We also analyzed the inhibitory effect of RHE on white adipose tissue (WAT) in high-fat diet (HFD)-induced obesity mice model. Male C57BL/6J mice were fed HFD or HFD supplemented with 1% RHE (HFDRH) for 8 weeks. The HFDRH-fed group gained less body weight and had less visceral fat than the HFD-fed group. Liver weight was significantly lower in the HFDRH-fed group and total hepatic lipid and triglyceride (TG) content was also reduced. A significant reduction in the expression of peroxisome proliferator-activated receptor gamma (PPAR${\gamma}$) was observed in epididymal fat in the HFDRH-fed group, in comparison with controls, through Western blotting. These results suggest that downregulation of PPAR${\gamma}$ expression is involved, at least in part, in the suppressive effect of RHE on lipid accumulation in WAT.