• Title, Summary, Keyword: TRC

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Cloning and Expression of a Chitinase Gene from Thermoactinomyces vulgaris KFB-C100

  • Yooh, Ho-Geun;Kim, Hee-Yun;Lim, Young-Hee;Cho, Hong-Yon
    • Journal of Microbiology and Biotechnology
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    • v.8 no.6
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    • pp.560-567
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    • 1998
  • We have found that Thermoactinomyces vulgaris KFB-Cl00 produces a chitinase. The optimum temperature and pH of the enzyme activity were $55^{\circ}C$ and 6.5. The enzyme was stable after heat treatment at $80^{\circ}C$ for 30 min and stable in acidic and basic conditions (PH 6.0~11.0). The thermostable endo-chitinase from Thermoactinomyces vulgaris KFB-C100 was cloned into the plasmid pBR322 by using E. coli DH5$\alpha$ as a host strain. The positive clone carrying a recombinant plasmid (PKCHI23) with a 4.1-kb fragment containing the chitinase gene was found. The recombinant plasmid was analyzed to determine the essential region for chitinase activity and obtained a 2.3-kb fragment, which was sub cloned into pTrc99A using the PstI and SalI sites to construct pTrc99A/pKCHI23-3. The resulting plasmid exerted high chitinase activity upon transformation of E. coli XL1-Blue cells. Chitinase was overproduced 14 times more in the clone cells than in the wild-type cells and the enzyme was purified to homogeneity. The purified enzyme showed the similar properties as the native chitinase from T. vulgaris in terms of molecular weight and substrate specificity. The catalytic action of the cloned enzyme was an endo type, producing chitobiose as a major reaction product.

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Cloning and Expression of Thermostable Chitosanase Gene from Bacillus sp. KFB-C108

  • Yoon, Ho-Geun;Kim, Hee-Yun;Kim, Hye-Kyung;Kim, Kyung-Hyun;Hwang, Han-Joon;Cho, Hong-Yon
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.631-636
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    • 1999
  • The thermostable endo-chitosanase gene from the isolated strain Bacillus sp. KFB-C108 was identified on the basis of a phylogenetic analysis of the 16S rRNA gene sequence, and was cloned into plasmid pUCl8 using E. coli $DH5\alpha$ as the host strain. Positive clones carrying recombinant plasmids (pKCHO I and pKCHO II) containing chitosanase activity were selected using the direct activity staining method. Detailed physical maps showed the two plasmid inserts were identical except that the KCHO II insert (2.6 kb) was 1.8 kb smaller than that of the KCHO I. The recombinant plasmids were analyzed to determine the essential region for chitosanase activity, and a 1.3-kb fragment (KCHO-6) was subcloned into pTrc99A using the EcoRI and BamHI sites to construct pTrc99A/KCHO-6(pTrEB13). The resulting plasmid exerted high chitosanase activity upon transformation of E. coli $DH5{\alpha}cells$, overproducing about 20 times more in the cloned cells than in the wild-type cells. The cloned chitosanase protein exhibited the same molecular weight and catalytic activity similar to those of Bacillus sp. KFB-C108. The cloned enzyme was an endo-type that produced a chitosan tetramer as the major reaction product; however, it produced no monomers or dimers.

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Railway Undercrossing Construction Method for the Shindorim Station Platform Extension (신도림 역사 확장을 위한 철도횡단 건설공법 검토)

  • Yoo, Je-Nam;Koo, Ja-Kap;Lee, Hee-Yung
    • Proceedings of the Korea Concrete Institute Conference
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    • pp.887-890
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    • 2008
  • Shindorim Station is the most important transfer station which is crossed Seoul Metro Line-2 and national rail traffic Kyungbu, Honam and Kyungin Line etc.. There is the lagest transfer passengers and the greatest rail traffic. Therefore to solve the congeted station problem, Shindorim station extension project has been planned. This project has very difficult many problems. One of them is undercrossing the national rail traffic ground without open-cut excavation. There are many undercrossing construction methods in our country. But this project is required the best safety. So the best applicable methods are investigated, which are Front-Jacking, NTR and TRcM. In Design stage Front-Jacking with PRS method which is gound reinforcing is applied.

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Study on searching method of human errors accidents for case study of disaster database (재해 데이터베이스의 사례연구를 위한 휴먼에러 재해 검색방법에 관한 연구)

  • 한우섭
    • Journal of the Society of Disaster Information
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    • v.1 no.1
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    • pp.121-136
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    • 2005
  • Most human-error case of accident database is written by various description and expression because accident database is produced by two or more person. And extracted information by searching of database varies in researcher's judgment criteria and the capability. Furthermore, much time and effort are required to examine manually information related to the human error from each accident case. Accordingly, it is difficult to explore objectively the accidents relevant to the human-error from the accident data base which is accumulated enormously. In this study, to solve these problems, it was developed an searchig method which is not influenced by researcher's judgment criteria and capability. For this, human-error keywords were extracted from a Japanese-English dictionary to examine objectively the accident case related to human-error in data base. This searching method by the human-error keywords can be applicable in most accident databases, although a database will be accumulated in future. Also, using the searching technique of this research, knowledge obtained by searching result can be compared with other research's results by the same method. Although the number of accident case increasese, searching results from database have the objectivity because it is not necessary to modify the based searching method or change the human-error keywords. However, as subject of future investigation, it would be necessary that the extension and investigation on human-error keywords improve and the technique to enhance searching accuracy would be modified.

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A Context-Aware Engine for Mobile Platforms (모바일 플랫폼 상황이해엔진)

  • Lee Sun A;Lee Keon Myung;Lee Jee-Hyong
    • Journal of Korean Institute of Intelligent Systems
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    • v.15 no.3
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    • pp.300-305
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    • 2005
  • Context-aware intelligent services are essential in ubiquitous computing and intelligent robots environments, which make decisions on which services to start with the consideration of surrounding contexts. In the ubiquitous and intelligent environments, context-aware service engines should be light-weighted due to the resource restrictions on the devices. This paper presents a context-aware service engine which is designed for light-weighted devices. The context-aware service engine has been designed with special attention to improve the execution speed and to minimize the memory requirement.

Control Performance Comparison of Model-referenced and Map-based Control Method for Vehicle Lateral Stability Enhancement (차량 횡방향 안정성 향상을 위한 모델 참조 제어와 맵기반 제어 방법의 제어 성능 비교)

  • Yoon, Moonyoon;Baek, Seunghwan;Choi, Jungkwang;Boo, Kwangsuck;Kim, Heungseob
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.3
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    • pp.253-259
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    • 2014
  • This study proposes a map-based control method to improve a vehicle's lateral stability, and the performance of the proposed method is compared with that of the conventional model-referenced control method. Model-referenced control uses the sliding mode method to determine the compensated yaw moment; in contrast, the proposed map-based control uses the compensated yaw moment map acquired by vehicle stability analysis. The vehicle stability region is calculated by a topological method based on the trajectory reversal method. The performances of model-referenced control and map-based control are compared under various road conditions and driving inputs. Model-referenced control uses a control input to satisfy the linear reference model, and it generates unnecessary tire lateral forces that may lead to worse performance than an uncontrolled vehicle with step steering input on a road with low friction coefficient. The simulation results show that map-based control provides better stability than model-referenced control.

Binary Power Control for Sum Rate Maximization of Full Duplex Transmission in Multicell Networks

  • Vo, Ta-Hoang;Hwang, Won-Joo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • pp.583-585
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    • 2016
  • The recent advances in wireless networks area have led to new techniques, such as small cells or full-duplex (FD) transmission, have also been developed to further increase the network capacity. Particularly, full-duplex communication promises expected throughput gain by doubling the spectrum compared to half-duplex (HD) communication. Because this technique permits one set of frequencies to simultaneously transmit and receive signals. In this paper, we focus on the binary power control for the users and the base stations in full-duplex multiple cellulars wireless networks to obtain optimal sum-rate under the effect interference and noise. We investigate with a scenario in there one carrier is assigned to only one user in each cell and construct a model for this problem. In this work, we apply the binary power control by the its simplification in the implemented algorithm for both uplink and downlink simultaneously to maximize sum data rate of the system. At first, we realize the 2-cells case separately to check the optimal power allocation whether being binary. Then, we carry on with N-cells case in general through properties of binary power control.

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Robust Plan Generation and Dynamic Execution for Intelligent Web Service (지능적인 웹서비스를 위한 강건한 계획 생성과 동적 실행 방법)

  • Hwang, Gyeong-Sun;Lee, Seung-Hui;Lee, Geon-Myeong
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • pp.320-323
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    • 2007
  • 웹 서비스와 같은 분산된 환경에서, 특정 서비스를 수행하기 위해서는 원격의 컴퓨터나 사이트상에서 다중 에이전트들의 협업을 통해 이루어진다. 이때 서비스는 여러 에이전트들의 복잡한 행위들에 의해 구성된다. 또한 지능적인 서비스를 위해서는 에이전트들의 상태정보, 목적정보, 그리고 계획정보 등을 이용한다. 특히 계획정보는 에이전트들이 일련의 행위들로 구성된다. 하지만 계획수립을 위한, 기존 연구들 대부분은 정적으로 기술된 서비스 명세와 초기상태 정보를 이용하여 특정 목표를 만족시키는 단일 계획 생성 방법을 연구해왔다. 따라서 계획수립이 실행 도중에 기대하지 않은 네트워크 장애나 방해 등으로 서비스 수행을 실패하는 경우, 그 계획은 무효가 되고 다시 계획을 생성 해야만 한다. 그러나 다시 계획을 생성하기 위해서는 많은 시간을 소비하게 될 뿐만 아니라 태스크 중복이 불가피하므로 매우 비효율적이다. 이 논문에서는 강건한 계획수립과 그 계획을 실행하기 위한 효과적인 방법을 제안한다. 즉, 계획수립의 재생성을 피하기 위한 방법으로 단일 계획수립 대신에 실행 가능한 다중 계획들로 표현된 강건한 계획을 생성하는 것이다. 강건한 계획의 행위들이 실행되는 동안, 각 단계마다 실행 가능한 행위를 선택한 후, 그 행위를 실행한다. 그러나 선택된 행위가 실행결과를 낼 수 없을 경우, 대체 가능한 서브 계획 경로를 선택하여 실행한다. 강건한 계획을 표현하기 위해 페트리 넷 기반의 방법을 제안한다. 강건한 계획 생성 방법에서는 이용 가능한 모든 계획들을 입력으로 사용한다. 그 계획수립 방법은 HTN 계획수립기로 잘 알려진 JSHOP2 계획수립기내에 구현하였다. 계획 실행 방법으로는 주어진 강건한 계획에 대하여 행위들이 직접 실행하수 있도록 한다.며 용량에 의존하는 양상을 보였다. $H_2O_2$에 의해 유발(誘發)된 DNA의 손상은 catalase와 deferoxamine에 의해 억제되었지만 DPPD는 억제시키지 못했다. 배기음(排氣飮)은 $H_2O_2$에 의해 유발(誘發)된 ATP의 소실을 회복시켰다. 이러한 실험결과 $H_2O_2$에 의해 유발(誘發)된 세포(細胞)의 손상(損傷)은 지질(脂質)의 과산화(過酸化)와는 다른 독립적인 기전에 의해 일어남을 나타낸다. 결론 : 이러한 결과들로 볼 때 Caco-2 세포(細胞)에서 배기음(排氣飮)이 항산화작용(亢酸化作用)보다는 다른 기전을 통하여 Caco-2 세포안에서 산화제(酸化劑)에 의해 유발(誘發)된 세포(細胞)의 사망(死亡)와 DNA의 손상(損傷)을 방지할 수 있다는 것을 가리킨다. 따라서 본 연구(硏究)는 배기음(排氣飮)이 반응성산소기(反應性酸素基)에 의해 매개된 인체(人體) 위장관질환(胃腸管疾患)의 치료(治療)에 사용할 수 있을 가능성(可能性)이 있음을 제시하고 있다.에 이를 이용하여 유가배양시 기질을 공급하는 공정변수로 사용하였다 [8]. 생물학적인 폐수처리장치인 활성 슬러지법에서 미생물의 활성을 측정하는 방법은 아직 그다지 개발되어있지 않다. 본 연구에서는 슬러지의 주 구성원이 미생물인 점에 착안하여 침전시 슬러지층과 상등액의 온도차를 측정하여 대사열량의 발생량을 측정하고 슬러지의 활성을 측정할 수 있는 방법을 개발하였다.enin과 Rhaponticin의 작용(作用)에 의(依)한 것이며, 이는 한의학(韓醫學) 방제(方劑) 원리(原理)인 군신좌사(君臣佐使) 이론(理論)에서 군약(君藥)이 주증(主症)에 주(主)로 작용(作用)하는 약물(藥物)이라는 것을 밝혀주는

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A Study on the Appropriate Rebate Level of DSM Program (DSM 프로그램의 적정 리베이트 수준에 관한 연구)

  • 이창호;조인승;박종진
    • Proceedings of the Korea Society for Energy Engineering kosee Conference
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    • pp.69-74
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    • 1996
  • 본 연구에서는 DSM 프로그램 개발에 있어 가장 일반적인 수행수단으로 활용되고 있는 리베이트의 적정수준 결정을 위한 방법과 절차를 제시하였다. DSM 기술의 비용효과 분석에 있어서는 전력회사의 회피비용과 기술비용을 각각 편익과 비용지표로 산정하였으며, 이를 토대로 RIM 및 TRC 지표에 의해 상이한 리베이트 수준에서의 시뮬레이션을 반복적으로 시행하였다. 적용 대상 프로그램으로는 현재 국내에서 시행되고 있는 고효율 조명프로그램의 전자식 안정기를 택하였다.

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A Software for Cost-effectiveness Evaluation of DSM Programs (수요관리사업 실적 평가용 전산모형 개발)

  • Park, Jong-Jin;Rhee, Chang-Ho;Kim, Jin-O
    • Proceedings of the KIEE Conference
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    • pp.618-620
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    • 2003
  • This paper presents a software for cost-effectiveness evaluation of DSM programs (Dsm-Lite). The presented software can help the users to analyze the energy consumptions and peak reductions of DSM programs, evaluate the cost effectiveness for TRC and RIM, and identify the benefits and the costs for each Test. The Dsm-Lite software can also provide the effective information on avoided costs, electric rates, and rebates by year etc.

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