• Title, Summary, Keyword: TRAIL

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TRAIL and Effect of Irradiation on Apoptosis of Cancer Cells (TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과)

  • Lee, Jaeseob;Jang, Seongjoo
    • Journal of the Korean Society of Radiology
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    • v.10 no.6
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    • pp.387-393
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    • 2016
  • Tumor using the efficient concomitant radiotherapy and chemotherapy to remove, prior to surgery and, either reduce the size of the tumor after surgery, or was can be made smaller, Or excised tumor, in a way to be removed, most conventional surgical method is surgical excision surgery therapy. And methods reduce or tumor size, or smaller, chemotherapy can kill tumor is administered selectively anticancer agent which increases the radioactive susceptible to tumor cells, sensitive to susceptibility to radiation are those which make it possible to respond to, either TRAIL methods of various biological cytostatic can deform the protein, by deforming the structure of the protein help to cell death it is known. In this paper, the HCT-116 cells thought to be a cancer cell to analyze the interaction of TRAIL and radiation. Experimental results, single use of TRAIL and radiation, results were compared with the control group, it was found to have no significant effect on each cell proliferation and apoptosis. Conversely treated with TRAIL, when treated in parallel radiation, it was possible to know that the HCT-116 cells significantly apoptosis occurs, The proportion of G1 ratio G0 also was found to have increased. TRAIL conclusion is increased apoptosis radiation defensive cells can know that increased radiosensitivity, also possible to alter the cell cycle, reduce cell proliferation ability stepwise it was possible. TRAIL is increased apoptosis, decreased cell proliferative capacity, it is considered to be possible to use as a radiation sensitizer.

Perceived Benefits and Problems Associated with Urban Trails by South Korean and U. S. Trail Users (도시녹지(都市綠地) 내(內) 트레일 이용(利用)에 있어서의 편익(便益)과 문제점(問題點)에 관한 한·미간(間) 트레일 이용자(利用者) 인식(認識)의 비교(比較) 연구(硏究))

  • Lee, Ju-Hee;Ivy, Mark I.;Moore, Roger L.
    • Journal of Korean Society of Forest Science
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    • v.90 no.5
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    • pp.585-592
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    • 2001
  • Interest in the development of urban trails is growing in South Korea. River front trails have been developed in Seoul, Daegu and other major cities. Such trails, and the corridors in which they are developed, often provide a myriad of benefits to the community, including open space, exercise, and stress release. This trend mirrors development patterns found in the United States and European nations. This study examined differences in trail users perceptions of trail attributes, activity participation, willingness to pay for trail access, and perceptions of trail benefits between trail users in the United States and those in South Korea. Perceptions of trail users at three trail sites in the metropolitan area of Daegu were examined, and then were compared to results reported in several studies conducted in the U. S. While this research was exploratory, it did uncover many interesting differences in perceptions of trail attributes between trail users in the two countries. This information may provide insight into the development of greenway systems in South Korea.

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The Histone Deacetylase Inhibitor Trichostatin A Sensitizes Human Renal Carcinoma Cells to TRAIL-Induced Apoptosis through Down-Regulation of c-FLIPL

  • Han, Min Ho;Park, Cheol;Kwon, Taek Kyu;Kim, Gi-Young;Kim, Wun-Jae;Hong, Sang Hoon;Yoo, Young Hyun;Choi, Yung Hyun
    • Biomolecules & Therapeutics
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    • v.23 no.1
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    • pp.31-38
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    • 2015
  • Histone acetylation plays a critical role in the regulation of transcription by altering the structure of chromatin, and it may influence the resistance of some tumor cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) by regulating the gene expression of components of the TRAIL signaling pathway. In this study, we investigated the effects and molecular mechanisms of trichostatin A (TSA), a histone deacetylase inhibitor, in sensitizing TRAIL-induced apoptosis in Caki human renal carcinoma cells. Our results indicate that nontoxic concentrations of TSA substantially enhance TRAIL-induced apoptosis compared with treatment with either agent alone. Cotreatment with TSA and TRAIL effectively induced cleavage of Bid and loss of mitochondrial membrane potential (MMP), which was associated with the activation of caspases (-3, -8, and -9) and degradation of poly (ADP-ribose) polymerase (PARP), contributing toward the sensitization to TRAIL. Combined treatment with TSA and TRAIL significantly reduced the levels of the cellular Fas-associated death domain (FADD)-like interleukin-$1{\beta}$-converting enzyme (FLICE) inhibitory protein (c-FLIP), whereas those of death receptor (DR) 4, DR5, and FADD remained unchanged. The synergistic effect of TAS and TRAIL was perfectly attenuated in c-$FLIP_L$-overexpressing Caki cells. Taken together, the present study demonstrates that down-regulation of c-FLIP contributes to TSA-facilitated TRAIL-induced apoptosis, amplifying the death receptor, as well as mitochondria-mediated apoptotic signaling pathways.

Sensitization of TRAIL-resistant SK-Hep1 Human Hepatocellular Carcinoma Cells by Luteolin (SK-Hep1 인체 간암 세포에서 Luteolin에 의한 TRAIL 저항성 감소 효과)

  • Kim, Eun-Young;Kim, An-Keun
    • YAKHAK HOEJI
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    • v.56 no.1
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    • pp.35-41
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    • 2012
  • In this study, we examined the effect of luteolin to enhance TRAIL-induced anticancer effect in SK-Hep1 cells. We found that combined use of TRAIL with luteolin markedly enhanced the cytotoxicity compared to either agent alone by inducing apoptosis. Furthermore, combined treatment of TRAIL with luteolin significantly induced activation of death receptor pathway-related proteins as well as PARP-cleavage and activation of effector caspases. Also, our result indicated that upregulation of DR4 and DR5 by luteolin combination may contribute to enhanced susceptibility of SK-Hep1 cells to TRAIL.

TRAIL Based Therapy: Overview of Mesenchymal Stem Cell Based Delivery and miRNA Controlled Expression of TRAIL

  • Attar, Rukset;Sajjad, Farhana;Qureshi, Muhammad Zahid;Tahir, Fizza;Hussain, Ejaz;Fayyaz, Sundas;Farooqi, Ammad Ahmad
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.16
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    • pp.6495-6497
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    • 2014
  • Rapidly increasing number of outstanding developments in the field of TRAIL mediated signaling have revolutionized our current information about inducing and maximizing TRAIL mediated apoptosis in resistant cancer cells. Data obtained with high-throughput technologies have provided finer resolution of tumor biology and now it is known that a complex structure containing malignant cells strictly coupled with a large variety of surrounding cells constitutes the tumor stroma. Utility of mesenchymal stem cells (MSCs) as cellular vehicles has added new layers of information. There is sufficient experimental evidence substantiating efficient gene deliveries into MSCs by retroviral, lentiviral and adenoviral vectors. Moreover, there is a paradigm shift in molecular oncology and recent high impact research has shown controlled expression of TRAIL in cancer cells on insertion of complementary sequences for frequently downregulated miRNAs. In this review we have attempted to provide an overview of utility of TRAIL engineered MSCs for effective killing of tumor and potential of using miRNA response elements as rheostat like switch to control expression of TRAIL in cancer cells.

TRAIL in Combination with Subtoxic 5-FU Effectively Inhibit Cell Proliferation and Induce Apoptosis in Cholangiocarcinoma Cells

  • Sriraksa, Ruethairat;Limpaiboon, Temduang
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.16
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    • pp.6991-6996
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    • 2015
  • In the past decade, the incidence and mortality rates of cholangiocarcinoma (CCA) have been increasing worldwide. The relatively low responsiveness of CCA to conventional chemotherapy leads to poor overall survival. Recently, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL or Apo2L) has emerged as the most promising anti-cancer therapeutic agent since it is able to selectively induce apoptosis of tumor cells but not normal cells. In this study, we aimed to investigate the therapeutic effect of TRAIL in CCA cell lines (M213, M214 and KKU100) compared with the immortal biliary cell line, MMNK1, either alone or in combination with a subtoxic dose of 5-fluorouracil (5-FU). We found that recombinant human TRAIL (rhTRAIL) was a potential agent which significantly inhibited cell proliferation and mediated caspase activities (caspases 8, 9 and 3/7) and apoptosis of CCA cells. The combined treatment of rhTRAIL and 5-FU effectively enhanced inhibition of CCA cell growth with a smaller effect on MMNK1. Our finding suggests TRAIL to be a novel anti-cancer therapeutic agent and advantage of its combination with a conventional chemotherapeutic drug for effective treatment of CCA.

Use Impacts on Environmental Deteriorations of Trail and Campsite in Tokyusan National Park (덕유산 국립공원 등산로 및 야영장의. 환경훼손에 대한 이용영향)

  • 권태호;오구균;이준우
    • Korean Journal of Environment and Ecology
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    • v.7 no.2
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    • pp.241-251
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    • 1994
  • Use impacts on environmental deteriorations were studied on the four major trails and a campsite of Tokyuksan National Park in 1993. The entire width, bare width, maximum depth and slope of trail as the trail condition were significantly greater on the more heavily used trail. Percentages of rock-exposed, deepening, root-exposed points as the deterioration types of trail which were surveyed at the total of 106 points were higher and trail conditions were significantly different from those of non-deteriorated points. On the Paekryon trail, the damaged area more severe than Class 4 reaches about 910$m^2$/km and the use impact and deterioration on campsites were accelerated. The dominant trees of the the upper and lower layer in trail edge vegetation could be Quercus serrata and Acer pseudo-sieboldianum for Paekryon trail, Q. mongolica and Tripterigium regelii for the other trails. A. pseudo-sieboldianum, Q. serrata, Rhus trichocarpa, Fraxinus sieboldiana, Lespedeza maximowiczii, Symplocos chinensis for Pilosa were classified for tolerant species to use impacts.

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Use Pattern and Impacts on Environmental Deteriorations on and around Trails in Chuwangsan National Park (주왕산국립공원 등산로의 이용패턴 및 주변환경훼손에 대한 이용영향)

  • 권태호;오구균;이준우
    • Korean Journal of Environment and Ecology
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    • v.8 no.2
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    • pp.167-176
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    • 1995
  • Autumn visitors to Chuwangsan National Park was more than summer's. About 89% of total visitors used main trail but approximately 35% visited to the Third Falls. The entire width, bare width, maximum depth and slope of trail as the trail condition surveyed at the total of 103 points were significantly greater on the more heavily-used trail. Trail conditons of rock-exposed, root-exposed, deepening points as the deterioration types of trail were significantly different from those of non-deteriorated points. According to the topographic position of trail, severe difference of dominant trees in the edge vegetation was found and Lespedeza maximowiczii, Rhododendron yedoense and Fraxinus sieboldana were dominant in shrub layer of trail edge vegetation. The crown coverage, number of species and individuals of shrub layer in edge vegetation were generally higher than those in the other national parks with more heavily-used trail.

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The proteasome inhibition enhances apoptosis by P53 expression and the dissipation of mitochondrial transmembrane potential in TRAIL-resistant lung cancer cells (Proteasome 억제에 의한 P53의 발현과 미토콘드리아 막 전압의 소실로 TRAIL에 저항하는 폐암세포의 사멸 강화)

  • Seol, Jae-Won;Park, Sang-Youel
    • Korean Journal of Veterinary Research
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    • v.49 no.1
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    • pp.1-8
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    • 2009
  • The ubiquitin-proteasome mediated protein degradation pathway plays an important role in regulating both cell proliferation and cell death. Proteasome inhibitors are well known to induce apoptosis in various human cancer cell lines. We investigated the effect of combined treatment with proteasome inhibitor and TRAIL, and a possible mechanism of the enhancing apoptosis by the both treatment, on TRAIL-resistant non-small cell lung cancer. A549 cells were exposed to the N-Acetyl-Leu-Leu-Norleu-al (ALLN) as a proteasome inhibitor and then treated with recombinant TRAIL protein. In A549 cells under proteasome inhibition conditions by pretreatment with ALLN, TRAIL treatment significantly decreased cell viability compared to that ALLN and TRAIL alone treatment. Also, the both treatment induced cell damage through DNA fragmentation and p53 expression. In addition, the combined treatment of both markedly increased caspase-8 activation, especially the exposure for 2 h, and Bax expression and induced the dissipation of mitochondrial transmembrane potential in A549 cells. Taken together, these findings showed that proteasome inhibition by ALLN enhanced TRAIL-induced apoptosis via DNA degradation by activated P53 and mitochondrial transmembrane potential loss by caspase-8 activation and bax expression. Therefore, our results suggest that proteasome inhibitor may be used a very effectively chemotherapeutic agent for the tumor treatment, especially TRAIL-resistant tumor cell.

A Study on Exercise Physiology of Climbing in Step-trail -At Keumsan in Hallyo-Haesang National Park- (한려해상국립공원 계단형 등산로에서 등산의 운동생리학적 연구 - 금산지구를 중심으로 -)

  • 이준우;박범진;최윤호;김재수
    • Korean Journal of Environment and Ecology
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    • v.12 no.4
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    • pp.398-405
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    • 1999
  • This study was carried out to investigate exercise load of visitors and physical condition of stairway in the trail of Keumsan located in Hallyo-Haesang National Park. Total trail length of Keumsan is 2,126m, and the number of step-trail section are 15, and the total length of step-trail section is 1,000.4m. Average gradient of step-trail was 20~40%. But the gradient and length were 45.7% and 268.5m in the section 11, and 58.4% and 188.6m in the section 12. The greatest gradient was 67.8% in the section 13. During the climbing in trail, heart rate were 104~184beats/min.. And in the step-trail section 11, 12, and 13, IHR, ELI, and %HRmax were 166.5%, 89.1%, 92.9%, and 167.4%, 89.6%, 93.2%, and 157.8%, 84.5%, 89.9%, respectively.

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