• Title, Summary, Keyword: Synthesis

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Chicken Insulin-Like Growth Factor-I Stimulates Protein Synthesis of Chicken Embryo Myoblasts Cultured in Serum-Free Medium

  • Kita, K.;Okumura, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.1
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    • pp.17-20
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    • 2001
  • The effect of chicken IGF-I on protein synthesis of chicken embryo myoblasts cultured in serum-free medium was examined. When myoblasts were expanded to approximate 20-30% of well, the medium was changed to the serum-free medium including 0, 2, 20, 200 or 2000 ng/ml of recombinant chicken IGF-I. The culture medium including 10% fetal calf serum (FCS) was used as positive control. After 1 day of incubation, protein synthesis was measured by the incorporation of [$^3H$]-L-leucine. Thereafter cells were continued to incubate for further 18 hours, and the radioactivity in the protein was measured as an index of protein synthesis. The values for protein synthesis cultured in the serum-free medium without chicken IGF-I or with 2000 ng/ml of chicken IGF-I were the lowest. Protein synthesis was elevated with increasing chicken IGF-I concentration from 0 to 20 ng/ml. The values for protein synthesis in the 20 ng/ml and 200 ng/ml IGF-I groups were about half of that of the FCS group. The present study revealed that the potency of chicken IGF-I at the levels of 20 to 200 ng/ml to stimulate myoblast protein synthesis was about half of that of 10% FCS.

Selection and Analysis of Genomic Sequence-Derived RNA Motifs Binding to C5 Protein

  • Kim, Kwang-sun;Ryoo, Hye-jin;Lee, June-Hyung;Kim, Mee-hyun;Kim, Tae-yeon;Kim, Yool;Han, Kook;Lee, Seol-Hoon;Lee, Young-hoon
    • Bulletin of the Korean Chemical Society
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    • v.27 no.5
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    • pp.699-704
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    • 2006
  • Escherichia coli RNase P is a ribonucleoprotein composed of M1 RNA and C5 protein. Previously, analysis of RNA aptamers selected for C5 protein from a synthetic RNA library showed that C5 protein could bind various RNA molecules as an RNA binding protein. In this study, we searched cellular RNA motifs that could be recognized by C5 protein by a genomic SELEX approach. We found various C5 protein-binding RNA motifs derived from E. coli genomic sequences. Our results suggest that C5 protein interacts with various cellular RNA species in addition to M1 RNA.

Substructure Synthesis Method using Dynamic Reduction (동축소법을 이용한 부분구조합성법)

  • 박석주;박성현;김성우
    • Journal of the Korean Society of Marine Engineering
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    • v.24 no.1
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    • pp.82-87
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    • 2000
  • The component mode synthesis method(CMS) used for vibration analysis has demerit that error becomes larger, as degree of natural frequency grows higher. The reason of error occurrence is that Guyan's static reduction is used and the number of modes taken in each component is deficient. This paper proposes the substructure synthesis method using dynamic reduction to solve the problem from the component mode synthesis method. Computer simulation for the proposed method. FEM and the component mode synthesis method(CMS) on a rectrangular plate has been carried out to prove the avilability of the proposed method.

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Effective Generation of Lead Compounds by High Throughput Organic Synthesis: using Multipurpose Privileged Bezopyrans

  • Gong, Young-Dae;Seo, Jin-soo;Hwang, Jong-Yeon;Park, Ji-Yeon;Yoo, Sung-Eun
    • Proceedings of the PSK Conference
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    • pp.89-89
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    • 2003
  • Numerous lead compounds, based on multipurpose privileged structures, can be generated that address a variety of targets from a gene family of interest, irrespective of therapeutic area. Solid-phase organic synthesis has been emerged as a powerful technique in generating combinatorial libraries of small organic molecules useful for drug discovery. Heterocyclic compounds provide scaffolds on which pharmacophores can be arranged to yield potent and selective drugs and a variety of heterocycles have been synthesized on solid support. (omitted)

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Effect of Polyacetylene Compounds from Panax Ginseng on Macromolecule Synthesis of Lymphoid lukemia L1210 (인삼 포리아세틸렌 화합물이 Lymphoid lukemia L1210의 고분자물질 합성에 미치는 영향)

  • Kim, Young-Sook;Kim, Shin-Il;Hahn, Dug-Ryong
    • YAKHAK HOEJI
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    • v.32 no.2
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    • pp.137-140
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    • 1988
  • To investigate polyacetylene compounds isolated from petroleum ether extract of panax ginseng effect on the macromolecule synthesis, lympoid lukemia L1210 cell was incubated with them at 4, 8, 12,16 hours. Panaxydol, panaxynol and panaxytriol as cytotoxic substances inhibited the synthesis of macromolecule such as DNA, RNA and protein. Panaxydol which had the most potent cytotoxicity among these three compounds showed the strongest inhibitory effect on DNA, RNA and protein synthesis. For DNA and RNA synthesis, panaxynol and panaxytriol decreased the rate of inhibition with the incubation time but panaxydol had a strongest inhibitory effect at 16 hour incubation time. Protein synthesis was markedly inhibited by all these polyacetylene compounds. It was obserbed that there is a relationship between cytotoxicities of polyacetylene compounds and the inhibition of macromolecule synthesis.

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Effect of N-Methylmesoporphyrin IX on the Branch Point of the Tetrapyrrole Pathway in Pea (Pisum sativum L.) Chloroplasts

  • Yu, Gyung-Hee
    • BMB Reports
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    • v.28 no.6
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    • pp.523-526
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    • 1995
  • Administering ${\delta}-aminolevulinic$ acid (ALA) to isolated pea (Pisum sativum L.) chloroplasts resulted in an increase of heme synthesis in the heme branch of the tetrapyrrole pathway. At 0.1 mM ALA, in the presence of 1 mM $FeSO_4$ heme synthesis was stimulated up to 7 fold of that in the absence of $FeSO_4$. N-Methylmesoporphyrin IX (NMMP), a powerful inhibitor of ferrochelatase, inhibited heme synthesis by 95% at one micromolar concentration. The addition of A TP to the chloroplasts caused not only heme synthesis, but Mg-protoporphyrin IX synthesis in the chlorophyll branch of the tetrapyrrole pathway. In the presence of NMMP, however, inhibition of Mg-protoporphyrin IX synthesis was not observed whereas heme synthesis was inhibited completely.

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Fast Synthesis based on Ternary Universal Logic Module $U_h$ (3치 범용 논리 모듈 $U_h$에 의한 빠른 논리 합성)

  • 김영건;김종오;김흥수
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.31B no.1
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    • pp.57-63
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    • 1994
  • The logic function synthesis using ULM U$_h$ is constructed based on canonic Reed-Muller expansion coefficient for a given function. This paper proposes the fast synthesis algorithm using ULM U$_h$ for ternary function. By using circuit cost and synthesis method of proposed in this paper, order of control input variable minimum number of ULM U$_h$ can be decided in the synthesis of n-variable ternary function. Accordingly, this method enables to optimum circuit realization for ternary function synthesis using ULM ULM U$_h$ and can be applied to ternary function synthesis using ULM U$_h$. The complexity of search for select the order of all control input variables is (n+2)(n-1)/2.

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Comparison of Component Mode Synthesis and Substructure Synthesis Methods (구분모드합성법과 부분구조합성법의 비교)

  • Kwak, Moon-K.;Na, Sung-Soo;Bae, Byung-Chan
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • pp.1348-1353
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    • 2006
  • This paper is concerned with the comparison of component mode synthesis md substructure synthesis methods. Those two methods were developed for the dynamic analysis of complex structure. However, the difference between two methods in solving the dynamic problem has never been explained. In this paper, a structure consisting of two beams is considered for the application of two methods. The dynamic formulation shows the characteristics and differences of two methods. The procedure for choosing proper substructure modes in each method is explained in detail. In addition, the advantage of the substructure synthesis method is discussed based on the numerical example. Numerical examples show how two methods are applied to the addressed problem.

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Zeolite-Mediated Cation Exchange Enhances the Stability of mRNA during Cell-Free Protein Synthesis

  • Kim, You-Eil;Kim, Dong-Myung;Choi, Cha-Yong
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.3
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    • pp.258-261
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    • 2006
  • The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

Task Planning of Single Robot through LTL Synthesis (LTL Synthesis 를 통한 단일 로봇의 작업 계획)

  • Kwon, Ryoungkwo;Kwon, Gihwon
    • Proceedings of the Korea Information Processing Society Conference
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    • pp.295-298
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    • 2010
  • Linear Temporal Logic synthesis 는 LTL formula 로 표현된 요구 사항으로부터 그것을 만족하는 시스템을 만들어낸다. 이러한 synthesis 과정은 2EXPTIME-complete 이 요구 되지만 GR formula 라는 특수한 형태를 사용함으로써 복잡도를 Polynomial 시간으로 줄일 수 있다. LTL synthesis 는 작업 공간, 로봇이 취하는 센서 정보와 액션의 종류, 상위 수준의 작업 명세를 입력으로 받아 GR formula 형태로 변환하고, 기대되는 작업이 실현 가능하다면 그것을 성취할 수 있는 오토마타를 생성해낸다. Synthesis 알고리즘을 구현한 LTLMoP 라는 도구를 이용하여 LTL synthesis 과정을 보이고 화성 행궁의 미아 찾기 로봇 작업 계획을 구현한다. 마지막으로 시뮬레이션 과정을 통해 기대하는 작업을 성공적으로 성취할 수 있음을 보인다.

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