• Title, Summary, Keyword: Streptozotocin

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The Anti-diabetes and Vasoelasticity Effects of Mori Folium and Aurantii Fructus in Streptozotocin Induced Type II Diabetes Mellitus Model (Streptozotocin 유발 당뇨 백서에 상엽 지각(桑葉 枳殼) 혼합물의 항당뇨 및 혈관탄성개선 효과)

  • Park, Jung-Sup;Park, Chong-Hyeong;Jun, Chan-Yong;Choi, You-Kyung;Hwang, Gwi-Seo;Kim, Dong-Woo
    • The Journal of Internal Korean Medicine
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    • v.28 no.3
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    • pp.544-559
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    • 2007
  • Objectives : This study was designed to evaluate the anti-diabetes and vasoelasticity effects of Mori Folium and Aurantii Fructus in streptozotocin-induced type II diabetes mellitus model. Methods : The anti-diabetic effect of Mori Folium and Aurantii Fructus on rats induced with diabetes by streptozotocin was investigated through analyses of changes in body weight, blood glucose, urine volume of rats, viability of human umbilical vein endothelial cells(HUVECs), and elasticity of descending thoracic aorta in rats. The subjects in this study were divided into four groups(n=15): a normal group without any treatment (Con), a normal group with Mori Folium and Aurantii Fructus treatment(Con+P), a diabetes group induced by streptozotocin(STZ), and a Mori Folium and Aurantii Fructus treatment group under diabetes induced by streptozotocin(STZ+P). Rats were administered streptozotocin to induce diabetes. Results : The study showed that Mori Folium and Aurantii Fructus significantly reduced highly increased blood glucose levels(p<0.01) and prevented the diabetic rats from weight loss(p<0.01) and polyurea(p<0.05), Mori Folium and Aurantii Fructus also recovered decreased viability of HUVECs(p<0.01) and damaged elasticity of aorta induced by the streptozotocin (p<0.01). Conclusions: It was concluded from the results that Mori Folium and Aurantii Fructus have a distinct anti-diabetes effect and they also prevent damage of blood vessel induced by diabetes. resulting in prevention of cardiovascular diseases ascribed to diabetes.

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Effects of Liriopis Tuber Herbal-Acupuncture on Diabetes Mellitus Induced by Streptozotocin in Rat (맥문동약침액(麥門冬藥鍼液)이 Streptozotocin으로 유발된 흰쥐의 당뇨병에 미치는 영향)

  • Park, Jae-Young;Park, Hea-Su
    • Journal of Pharmacopuncture
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    • v.3 no.2
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    • pp.99-130
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    • 2000
  • This study was carried out to investigate the effects of Liriopis Tuber Herbal-Acupuncture on Diabetes Mellitus induced by streptozotocin in rat. Body weight, serum insulin, glucose, triglyceride, HDL cholesterol, free cholesterol and total protein were measured in streptozotocin induced diabetic rat injected with Liriopis Tuber Herbal-Acupuncture solution at Pisu(BL20) respectively for 30 days. In order to study the effects of Liriopis Tuber Herbal-Acupuncture at Pisu(BL20) on Diabetes Mellitus induced by Streptozotocin in rat, The groups were divided into five groups; Normal group(non treated group), N.-Saline group(administration of injection with normal saline at BL20 in 30 days after streptozotocin injection), Control group(non treated group after streptozotocin injection), H. Acup. group (administration of Liriopis Tuber Herbal-Acupuncture at BL20 in 30 days after streptozotocin injection), and Acup. group (administration of acupuncture at BL20 in 30 days after streptozotocin injection). The results obtained were summarized as follows; 1. As compared with Control group, there wae no significant tendency to diminish the rate of weight loss in H. - Acup. group. 2. As compared with Control group, there wae signiticant increase of serum insulin level in H. -Acup. group. 3. As compared with Control group, there was decrease of glucose level in H.- Acup. group. 4. As compared with Control group, there was decrease of triglyceride level in H. - Acup. group. 5. As compared with Control group, trere wae increase of HDL cholesterol level in H. - Acup. group. 6. As compared with Control group, there was significant increase of free cholesterol level in H. - Acup. group. 7. As compared with Control group, there was Increase of total protein level in H. - Acup. group. According to above mentioned results, Liriopis Tuber Herbal-Acupuncture was expected to be effective in treatment of Diabetes Mellitus and its complications.

Antidiabetic Effect of Glechoma longituba (Nakai) Kupr in Diabetic Rats induced by Streptozotocin (연전초(連錢草) 추출물이 Streptozotocin으로 유발시킨 흰쥐의 당뇨에 미치는 영향)

  • Lee, Kyung-Jin;Ham, In-Hye;Bu, Young-Min;Kim, Ho-Cheol;Choi, Ho-Young
    • The Korea Journal of Herbology
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    • v.23 no.3
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    • pp.175-180
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    • 2008
  • Objecitivies : While Glechoma longituba (Nakai) Kupr(GL) has been known as a antiinflammatory, antidiabetic and antihypertension medicine in korea traditional medicine, there has been only a few researches about it in Korea. Thus, we examined antidiabetic effect of GL in diabetic rats induced by streptozotocin Methods : To verify the effects of GL on serum glucose, creatinine, BUN, total cholesterol and triglyceride through streptozotocin-induced diabetic rats, some rats were injected 1 time with 55mg/kg streptozotocin intraperitoneally to induce diabetes, and other rats were injected with buffer I.P. as control group. Results : GL extracts significantly reduced the amount of serum glucose, BUN, creatinine, and triglyceride level in diabetics rats induced by streptozotocin. Conclusions : According to above result, Glechoma longituba was expected to be applied to the treatment of diabetes mellitus.

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Effects of Sophorae Flos on Steptozotocin-induced Diabetic Mellitus in Rats (괴화(槐花) 추출물의 투여(投與)가 Streptozotocin으로 유발된 당뇨쥐에 미치는 영향)

  • Kim, Hyun-Ji;Kim, Kyung-Jun
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.20 no.1
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    • pp.51-65
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    • 2007
  • Objective : The present study was carried out to investigate the preventive effect of Sophorae Flos on streptozotocin - induced Diabetes mellitus. Method : Sophorae Flos was given to rats with oral administration. The experimental animals were divided into 3groups : normal group of rats, control group of Streptozotocin-induced diabetic rats, sample group with Sophorae Flos . The effect of Sophorae Flos on Streptozotocin-induced diabetes was observed by measuring the survival rate of rats, weight of rats, FER, blood glucose, each organ weight of rat, total cholesterol, HDL, GOT, GPT & creatinine. Result : Streptozotocin caused hyperglycemia and hypoinsulinemia by a selectively destroying pancreatic ${\beta}-cell$. Sophorae Flos treatment don't protected them from hyperglycemia and hypoinsulinemia. Organ weight liver, kidney, heart & spleen shows no significant changes. Sophorae Flos significantly don't recoverd the increase of several biochemical parameters such as blood glucose, total cholesterol, HDL, GOT, GPT, antioxidant & creatinine is vice versa. Conclusion : Sophorae Flos extract group did not show significant decrease than Streptozotocin control group.

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Effects of Silkworm Extract on Streptozotocin-induced Diabetic Rats

  • Han, Sang-Mi;Suk, Young-Sik;Baek, Ha-Ju;Park, Hyun-Ro;Han, Myung-Sae
    • International Journal of Industrial Entomology
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    • v.5 no.2
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    • pp.201-204
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    • 2002
  • This study was undertaken to find effects of ethanol extracts from Bombyx mori on the hyperglycemia induced by streptozotocin in rats. Blood glucose level of rats increased depending on experimental days after streptozotocin treatment. But there was no difference in blood glucose level between the rats injected with ethanol extracts of B. mori and normal rats. There was no significant difference in the kidney fibrosis between the rats injected with ethanol extrscts of B. mori and normal rats at all ages examined. The transforming growth factor-$\beta$l ($TGF-{\beta}1$) protein was detected in the glomerulay endothelial cells and tubular epithelial cells of streptozotocin rats at 14 days of age. In the rats injected with ethanol extracts of B. mori to the kidneys, the $TGF-{\beta}1$ protein was detected very faintly. The $TGF-{\beta}1$ mRNA of streptozotocin rats increased at 14 days of age, and this was higher than the rats injected with ethanol extrscts of B. mori. Taken these together, the results suggest that the ethanol extracts of B. mori ameliorate hyperglycemia of the rats induced by streptozotocin.

Effects of Saengjinyanghyutang-gamibang and Rosa rugosa on the Pancreas in the Streptozotocin Treated Mice for Induction of Diabetes (생진양혈탕가미방과 매괴화가 Streptozotocin으로 유발된 고혈당 생쥐의 췌장에 미치는 영향)

  • 홍광표;나창수;장경선;김희철;박민희;김정상
    • The Journal of Korean Medicine
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    • v.22 no.4
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    • pp.79-89
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    • 2001
  • Objectives : This study has been carried out to investigate the effects of the Saengjinyanghyutang-gamibang and Rosa rugosa extracts on pancreas of the hyperglycemia mice induced with streptozotocin. Methods : We examined immunohistochemistry for insulin and COX-2, ultrastructural changes of acini by electron microscope, and changes of the blood glucose and BUN levels. Results : The ${\beta}-cells$ on Langerhnan's islet were destructed by administration of streptozotocin, so that few insulin-positive cells were observed in the control group. However, a lot of insulin-positive cells were observed in the experimental groups. These cells had recovered from the damage. As a result of COX-2 immunostain, COX-2 expression were highest in the control group other than the Saengjinyanghyutang-gamibang and Rosa rugosa extracts administered groups. As the electron microscopical observation, the centroacinar cells and acini of pancreas were destructed or damaged by administration of streptozotocin in the control group, but these recovered !Tom the damage in the other experimental groups. The levels of serum glucose were decreased remarkably on the Rosa rugosa and Saengjinyanghyutang-gamibang extracts administered groups compared with control group. Conclusions : These results suggest that administration of the Rosa rugosa and Saengjinyanghyultang-gamibang extracts to the mice reduced the damage induced by streptozotocin.

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Effects of Chungpyesagantang on the Diabetic Rats induced by Streptozotocin (청폐사간탕(淸肺瀉肝湯)이 Streptozotocin으로 유발(誘發)된 흰쥐의 실험적(實驗的) 당뇨(糖尿)에 미치는 영향(影響))

  • Koo Jin-Suk;Kim Jang-Hyeon
    • The Journal of Pediatrics of Korean Medicine
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    • v.11 no.1
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    • pp.227-248
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    • 1997
  • In order to study the effects of Chungpyesagantang(淸肺瀉肝湯) on the diabetic rats induced by streptozotocin, during 15 days rats were administered Chungpyesagantang extract after streptozotocin injection (50mg/kg) (sample group). On 2nd, 9th, and 15th day, I investigated the levels of body weight, serum glucose, serum total cholestrol, serum triglyceride. The last day, I killed rats and investigated hepatic lipid peroxidase. The results were obtained as follows: 1. Body weight of the diabetic rats induced by streptozotocin and Chungpyesagantang, as compared with the control group on the 15th day, increased effectively. (p〈0.05 respectively) 2. Glucose levels in serum of the diabetic rats induced by streptozotoc in and Chungpyesagantang, as compared with the control group on the 9th and 15th day, decreased effectively.(p〈0.05 respectively) 3. Total cholesterol levels in serum of the diabetic rats induced by streptozotocin and Chungpyesagantang, as compared with the control group on the 9th and 15th day, decreased effectively.(p〈0.05 respectively ) 4. Triglyceride levels in serum of the diabetic rats induced by streptozotocin and Chungpyesagantang, as compared with the control group on the 9th and 15th day, decreased effectively.(p〈0.05 respectively) 5. On hepatic lipid peroxidethe of the diabetic rats induced by streptozotocin and Chungpyesagantang, as compared with the control group on the 9th and 15th day, decreased effectively.(p〈0.01 respectively)

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Allomyrina Dichotoma Larva Extracts Protect Streptozotocin-induced Oxidative Cytotoxicity (Allomyrina Dichotoma Larva 추출물이 췌장 ${\beta}$-세포에서 streptozotocin에 의해 유도된 산화적 손상에 대한 보호효과)

  • Kim, Deok-Song;Huh, Jin;You, Guen-Chang;Chae, Soo-Chul;Lee, Oh-Sun;Lee, Hwang-Hee Blaise;Lee, Jong-Bin;Kim, Jong-Sun
    • Environmental Analysis Health and Toxicology
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    • v.22 no.4
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    • pp.349-355
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    • 2007
  • 장수풍뎅이 유충(Allomyrina dichotoma larva, ADL)은 중국의 전통 약재로서, 특히 항산화 효과가 우수하여 항당료 제재로 사용되고 있다. 본 연구에서는 이러한 ADL의 추출물을 이용하여 헴스터 췌장의 ${\beta}$-세포(HIT-T15)에서 Streptozotocin에 의해 유발된 산화적 손상에 대한 보호효과 및 그 작용기전을 조사하였다. ADL추출물은 처리농도 의존적으로 Streptozotocin에 의해 유발된 지질과산화 및 세포 내 자유산 소종의 양을 억제함으로서 ${\beta}$-세포의 산화적 스트레스에 의한 손상을 보호하였다. 또한 DNA laddering 방법을 사용하여 Streptozotocin에 의해 유발된 DNA 손상을 조사한 결과, ADL추출물 처리농도에 비례하여 Streptozotocin에 의해 유발된 DNA 손상이 감소하였다. 이러한 산화적 손상의 억제능 관련 작용 기전을 조사하기 위해 DPPH free radical 소거능을 실시하였다. 그 결과 ADL추출물 자체가 DPPH 자유 레디컬 소거능이 있음을 확인하였으며, 또한 플라스미드를 이용한 Single-strand break 방법을 통한 DNA 손상 보호능을 측정한 결과도 $Fe^{3+}$$H_2O_2$에 의해 유발된 DNA 손상이 ADL추출물 처리농도에 비례하여 감소하였다. 이러한 결과들을 종합하여 볼 때, 장수풍뎅이 유충의 추출물들이 자체의 레디컬 소거능 및 산화적 손상에 의한 DNA손상을 억제함으로써, Streptozotocin에 의해 유발된 산화적 손상을 억제할 수 있을 것이라 사료된다.

Effects of Hwangryungeehwang-tang Aqueous Extracts on Streptozotocin-induced Diabetic Rat (황련지황탕(黃連地黃湯)이 Streptozotocin으로 유발(誘發)된 당뇨병(糖尿病)에 미치는 영향)

  • Yu, Dong-Jo;Yoon, Gyeong-Min;Kang, Seok-Bong
    • The Journal of Internal Korean Medicine
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    • v.32 no.2
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    • pp.288-300
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    • 2011
  • Objectives : The object of this study was to observe the effects of Hwangryungeehwang-tang (HRGHT), which has traditionally been used in Korean medicine for treating diabetes, by administering aqueous extracts to streptozotocin-induced diabetic rats. Methods : Three different dosages of HRGHT extracts were orally administered once a day for 28 days from 3 weeks after streptozotocin treatment (60 mg/kg, single intraperitoneally administered). 6 groups, of 8 rats per group were used in the present study, selected 3 weeks after streptozotocin treatment based on blood glucose levels and body weights. Changes to the body weights, blood glucose levels, serum low density lipoprotein (LDL), high density lipoprotein (HDL), triglyceride and total cholesterol levels were observed, as well as changes on the pancreatic, hepatic and kidney malondialdehyde (MDA) contents for lipid peroxidation and glutathione contents for antioxidant effects. The results were compared with a potent antioxidant silymarin 100 mg/kg, of which the effects on streptozotocin-induced diabetes and related complications are already confirmed. Results : Significant decrease of blood glucose levels, serum LDL, total cholesterol, triglyceride levels, pancreatic, hepatic and renal MDA contents and significant increase of body weights, serum HDL levels, pancreatic, hepatic and renal GSH contents were detected in HRGHT extracts 100 and 200 mg/kg administered groups as compared to the streptozotocin control group. The lowest dosage of HRGHT treated group, 50 mg/kg, did not show any favorable effects as compared with the streptozotocin control. Conclusions : HRGHT has favorable effects on diabetes and related hyperlipidemia. Therefore, more detailed mechanism studies should be conduced in future with efficacy tests of individual herbal compositions of HRGHT and screening of the biological active compounds in the herbs.

Fine Structure of Suxtaglomerular Cells in Streptozotocin-treated Normal and Genetically Diabetic KK Mice (Streptozotocin이 정상 ICR 생쥐 및 유전성 당뇨병을 가진 KK생쥐 사구체곁세포의 미세구조에 미치는 영향)

  • 최월봉;최창도원무호김남주
    • The Korean Journal of Zoology
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    • v.35 no.3
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    • pp.372-382
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    • 1992
  • 당뇨병유발제인 streptozotocin이 생쥐 신장 사구체곁세포의 미세구조에 어떠한 영향을 미치는 지를 알아보고자 일반계통인 ICR생쥐와 유전성 당뇨병계통인 KK생쥐에 streptozotocin을 투여하여 경시적으로 각 동물의 신장 사구체곁세포의 미세구조의 변화를 관찰하였다. Streptozotocin을 투여한 ICR생쥐의 사구체곁세포는 3일째부터 과립형질내세망의 미약한 팽창과 과립내에 대소 공포의 출현 및 용해소체가 간혹 관찰되었다. 그후 시간이 지남에 따라 더욱 심하여 특히 2주 및 4주에서는 과립형질내세망의 팽창, 사립체, 골지장치 및 리보소곤 등이 소수 출현하였는데 비해 대소 용해소체는 많이 관찰되었으며 심한 탈과립으로 인해 세포질내 과립의 면적이 현저히 감소되었다. 그러나 KK생쥐의 실험군에서는 전 실험군에 걸쳐 퇴행성변화가 적었으며 ICR 생쥐 실험군에 비해 그 영향이 훨씬 적었다. 이상의 결과를 종합하여 보면 정상 ICR생쥐에 streptozotocin을 투여하자 되면 ICR생쥐 사구체곁세포에서 과립의 유의한 감소 및 세포내 미세구조의 퇴행성변화가 뚜렷한데 비해 KK생쥐 실험군에서는 ICR생쥐 실험군에 비해 손상을 적게 받았는데 이는 KK생쥐가 갖고 있는 당뇨병에 대한 내성에 의해 영향을 적게 미치는 것이 아닌가 추측된다.

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