• Title, Summary, Keyword: Steinernema carpocapsae

Search Result 66, Processing Time 1.59 seconds

Pathogenicity of Entomopathogenic Nematode, Steinernema carpocapsae against Fall Webworm, Hyphanria cunea (Lepidoptera: Arctiidae) (미국흰불나방(Hyphanria cunea)에 대한 곤충병원성선충 Steinernema carpocapsae의 병원성)

  • Park Hyung Soon;Kim Hyeong Hwan;Chung Hun Gwan;Cho Yoon Sin;Jeon Heung Yong;Jang Han Ik;Kim Dong Soo;Choo Ho Yul
    • Asian Journal of Turfgrass Science
    • /
    • v.18 no.4
    • /
    • pp.193-200
    • /
    • 2004
  • Environmentally sound control of fall webworm, Hyphanria cunea (Drury) with entomopathogenic nematode, Sreinernema carpocapsae Pocheon strain was evaluated in the laboratory and pot. Pathogenicity of 5. carpocapsae Pocheon strain was different depending on larval stage, i.e., mortality of the 2nd instar and the $3\~4th$ instar was $100\%$ with >20 infective juveniles (Ijs)/larva in 3 days, but the 5th instar was $34\%$ with (Ijs)/larva in 3 days. Pathogenicity of 5. carpocapsae Pocheon strain was higher with increasing nematode concentration. Mortality of Hyphanria cunea larva by 5. carpocapsae Pocheon strain was not significantly different (more than $70\%$) between nematode concentration on treated trees (Malus alba and Platanus orientalis) and in pot.

Biological Control of Apple Pests with Entomopathogenic Nematodes, Steinernema spp. (Steinernema 속 곤충병원선충을 이용한 사과원 병해충의 생물학적 방제)

  • 유연수;박선호
    • KSBB Journal
    • /
    • v.15 no.1
    • /
    • pp.106-111
    • /
    • 2000
  • Peach fruit month, smaller tea tortrix, and Melotontha incana are major pests of apple and apple trees throughout the country. In this work, we examined efficacies of entomopathogenic nematodes Steinernema carpocapsae and Steinernema glaseri against these apple pests. Steinernema carpocapsae showed 100% mortality after 24hr against peach fruit moth when it was applied on the larva with the concentration of 80 nematodes per larva, but Steinernema glaseri caused 83.3$\pm$5.8% mortality after 24hr at the concentration of 50 nematodes per larva. In the case of smaller tea tortrix, S. carpocapsae and S. glaseri caused 100%, 43.3$\pm$5.8% at the concentration of 50 nematodes per larva after 48 hr, respectively. However, 5~6 instar of Melotontha incana was not killed by treatments with S. carpocapsae and S.glaseri up to concentration of 200~800 nematodes per larva. The motility of nematodes in a soil increased as both inoculation concentration of nematode per larva and temperature increased. The mortality of G. mellonella by S. carpocapsae was 100% up to 10cm in depth and 56.7$\pm$5.8% at 10~15cm in depth when the temperature was $25^{\circ}C$ and 50 nematodes per larva were used.

  • PDF

Noneffective Results of Steinernematid and Hoterorhabditid Nematodes Agains Pill bug, Armadillidium vulgare (Isopoda : Armadillidae) (Steinernematid와 Heterorhabditid 선충의 쥐며느리에 대한 비효용적결과)

  • 추호렬;이동운;허은영;김준범
    • Korean journal of applied entomology
    • /
    • v.35 no.1
    • /
    • pp.91-93
    • /
    • 1996
  • Steinemematid and heterorhabditid nematodes were not effective to control the piU bug, Armadillidium vulgare although these nematodes were able to infect pill bugs. Steinernema carpocapsae Pocheon strain and S. glaseri Dongrae strain were more effective than S. carpocapsae AU strain or Heterorhabditis bacteriophora. Nematode concentration was more important factor than host density to develop infectivity.

  • PDF

Leaf Spray Control Efficacy of the Entomopathogenic Nematode, Steinernema carpocapsae Weiser, Supplemented with the Selected Antidesiccant, Keltrol-F, on the Beet Armyworm, Spodoptera exigua(Hubner) (선발 내건제 Keltrol-F를 이용한 곤충병원선충(Steinernema carpocapsae Weiser)의 파밤나방(Spodoptera exigua(Hubner))에 대한 엽면살포 방제 효과)

  • 이성섭;김용균;한상찬
    • Korean journal of applied entomology
    • /
    • v.39 no.3
    • /
    • pp.199-205
    • /
    • 2000
  • The field control efficacy of entomopathogenic nematode, Steinernema carpocapsae Weiser, was evaluated on the beet armyworm, Spodoptera exigua (Hiibner). The insect pest has been known to be a defoliator at the aerial part of the crop and difficult to be controlled effectively with most commercial chemical insecticides due to its insecticide resistance. To overcome the susceptibility of the nematodes to desiccation when they were applied by leaf spray in field condition, we screened several commercial antidesiccants (alkyl glucoside, CMC, glycerol, Keltrol-F, Kunipia-G, and Laponite LXG) optimal for survival of the nematodes. Keltrol-F (0.1 %) was selected as a candidate supplement for field application of the nematodes. Leaf spray of the nematodes at 5,000 infective juveniles/ml of distilled water containing 0.1 % Keltrol-F resulted in 87.7% control efficacy on the 3rd instar larvae of Sp. exigua.

  • PDF

Pathogenicity and Multiplication of Entomopathogenic Nematode, Steinernema carpocapsae Weiser, on Been Armyworm, Spodoptera exigua (Hubner) and Tobacco Cutworm, Spodoptera litura(Fabricius) (파밤나방(Spodoptera exigua (Hubner))과 담배거세미나방(Spodoptera litura(Fabricius))에 대한 곤충병원선충(Steinernema carpocapsae Weiser)의 감염력 및 증식력)

  • 한상찬;이성섭;김용균
    • Korean journal of applied entomology
    • /
    • v.38 no.3
    • /
    • pp.255-260
    • /
    • 1999
  • Pathogenicity and multiplication of entomopathogenic nematode, Steinernema carpocapsae Weiser, ere analyzed in two insect hosts, Spodoptera exiga (Hubner) and Sp. litura(Fabricius). The estimated ${LC}^{50}s$(lethal concentration of the infective juveniles to kill 50% of the host insect population) were not different between tow insect species or among their developmental stages on the filter paper assay, thought the actual numbers of the infected nematodes were varied among them. The significant variation, however occurred in nematode multiplication between tow inset hosts. Temperature also gave sosignificatn effect on nematode multiplication rate that it took 6 days after infection at $25^{\circ}C$, but did 12 days at $20^{\circ}C$ to show the maximal nematode population peak($\approx$500,000 infective juveniles(IJ) in a 5th instar larva of Sp. litura and $\approx$-100,000 IJ in a 5th instar larva of Sp. exigua).

  • PDF

Biological Control of Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae) by Steinernematid and Heterorhabditid Entomopathogenic Nematodes (Steinernematid와 Heterorhabditid 곤충병원성 선충을 이용한 담배거세미나방 (Spodoptera litura)의 생물적 방제)

  • Kim, Hyeong-Hwan;Cho, Sung-Rae;Choo, Ho-Yul;Lee, Sang-Myeong;Jeon, Heung-Yong;Lee, Dong-Woon
    • Korean journal of applied entomology
    • /
    • v.47 no.4
    • /
    • pp.447-456
    • /
    • 2008
  • Five effective strains against tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Steinernema carpocapsae (GSN1), Steinernema sp. (GSNUS-10), Steinernema sp. (GSNUS-14), Heterorhabditis bacteriophora Hamyang (HbH), and Heterorhabditis sp. (GSNUH-1) were selected among 14 isolates of Korean entomopathogenic nematode in laboratory tests. $LC_{50}$ values of above five strains against tobacco cutworm were various by different nematode strains and developmental stages of tobacco cutworm. $LC_{50}$ value of S. carpocapsae (GSN1) was the lowest by $4.0{\sim}8.3$ infective juveniles (Ijs) and 2nd instars of tobacco cutworm was most susceptible. Pathogenicity of five effective strains against tobacco cutworm depends on nematode strain, concentration, and application times. The most effective strain was determined as S. carpocapsae (GSN1). Two or three times of applications were effective regardless of nematode strain, or concentration. Efficacy of S. carpocapsae (GSN1), Steinernema (GSNUS-10), Steinernema (GSNUS-14), and Heterorhabditis (GSNUH-1) was variable depending on nematode strain, concentration, application times, and host variety. S. carpocapsae (GSN1) was the most effective and inoculation of 100,000 infective juveniles per m2 (720,000 Ijs/7.2 $m^2=1{\times}10^9$ Ijs/ha) resulted in higher efficacy. Three times of application of nematodes led to higher control efficacy than one or two applications. Efficacy of nematodes was higher on Chinese cabbage than cabbage or kale.

Pathogenicity of Entomopathogenic Nematode, Steinernema carpocapsae GSN1 Strain (Rhabditida: Steinernematidae) against Tebenna issikii (Lepidoptera: Choreutidae) (우엉뭉뚝날개나방(Tebenna issikii)에 대한 Steinernema carpocapsae GSN1 계통의 병원성)

  • Kim, Hyeong-Hwan;Han, Gun-Yeong;Choo, Ho-Yul;Lee, Sang-Myeong;Lee, Dong-Woon
    • Korean journal of applied entomology
    • /
    • v.46 no.2
    • /
    • pp.313-318
    • /
    • 2007
  • Entomopathogenic nematode, Steinernema carpocapsae GSN1 strain was evaluated for the environmentally sound control of Tebenna issikii (Lepidoptera: Choreutidae) in the laboratory. The corrected mortality of Tebenna issikii larvae was 100% at the 40 infective juveniles (Ijs)/larva 3 days after treatment with S. carpocapsae GSN1 strain in Petri dish. $LC_{50}$ value of S. carpocapsae GSN1 strain against Tebenna issikii was 5.7 Ijs. The mean penetration numbers of Ijs of S. carpocapsae GSN1 strain at the 5, 10, 20, 40 and 80 Ijs/larva in a Tebenna issikii larva were 1.4, 1.4, 3.2, 5.6 and 11.9 Ijs/larva, respectively. However penetration rate of Ijs of S. carpocapsae GSN1 strain at 5 Ijs/larva was the highest among other nematode concentrations. Progeny of S. carpocapsae GSN1 strain in a Tebenna issikii larva was higher with increasing nematode concentration.

Isolation and Identification of a Symbiotic Bacterium from Steinernema carpocapsae

  • Park, Sun-Ho;Yu, Yeon-Su
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.4 no.1
    • /
    • pp.12-16
    • /
    • 1999
  • Xenorhabdus nematophilus sp., an insect-pathogenic bacterium, was newly isolated from Korean entomopathogenic nematode of Steinernema carpocapsae, which can be used as a useful bioinsecticide. Primary and secondary form variants of Xenorhabdus nematophilus were observed when cultured in vitro. Primary form variants adsorbed bromothymol blue, while secondary form did not. However, many other characters of two variants were very similar. The variants were all rod-shaped and cell size was highly variable ranging from 0.5 by 2.0 ${\mu}$m to 1.0 by 5.0 ${\mu}$m. Both produced highly toxic substances and killed the insect larva within 20∼38 hr, indicating that insect pathogenicity of Xenorhabdus is not directly associated with its phase variation. In addition, cell-free culture supernatant of Xenorhabdus was sufficient to kill the insect larva by injecting it ito insect hemolymph; however, cell-harboring culture broth was more effective for killing the insect. The use of Xenorhabdus nematophilus may provide a potential alternative to Bacillus thuringiensis (Bt) toxins.

  • PDF

Biological Control of the Sciarid Fly, Lycoriella mali (Diptera: Sciaridae) Using Steinernema carpocapsae in a Button Mushroom Cultivation House (양송이버섯 재배사에서 Steinernema carpocapsae를 이용한 긴수염버섯파리 생물적 방제)

  • Choi, Yong-Seok;Seo, Hwa-Young;Whang, In-Su;Lee, Dae-Hong
    • Korean journal of applied entomology
    • /
    • v.57 no.4
    • /
    • pp.355-359
    • /
    • 2018
  • We used the entomopathogenic nematode, Steinernema carpocapsae, for the effective biological control of Lycoriella mali during Agaricus bisporus cultivation. The concentrations of two S. carpocapsae products distributed in Korea were $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ respectively. As a result of investigating the density of A. bisporus larvae in a rice straw medium and soil in an A. bisporus cultivation house, the mean numbers per $30cm^2$ were 0.8 and 22.2, respectively, and A. bisporus larvae were detected mainly in the soil. The control effects after once spraying S. carpocapsae at $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ was higher at the 14th day than at the 7th day. Therefore, we investigated the control effect after twice spraying of $6{\times}10^4$ and $2.5{\times}10^5m^{-2}$ at the 14th day. The control effects after this spraying were 51.9% and 96.8%, respectively, and the control effect of spraying at $2.5{\times}10^5m^{-2}$ was better than at $6{\times}10^4m^{-2}$. The control effect of diflubenzuron WP was lower than 50%.